TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3478999.2.1
(825 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AW698442.1|AW698442 G296 glandular-haired subtracted cDN... 246 2e-066
gb|AW698617.1|AW698617 g513 glandular-haired subtracted cDN... 234 9e-063
gb|CO514742.1|CO514742 s13dSG55F0200027_328144 Glandular tr... 176 3e-045
gb|CO512153.1|CO512153 s13dSG34E0900067_108626 Glandular tr... 173 3e-044
gb|CO514801.1|CO514801 s13dSG59D0300030_328262 Glandular tr... 173 3e-044
gb|CO511830.1|CO511830 s13dSG02E0400035_103560 Glandular tr... 166 2e-042
gb|CO515876.1|CO515876 s13dSG91B0900075_421291 Glandular tr... 165 6e-042
gb|CO512082.1|CO512082 s13dSG18F0600047_108484 Glandular tr... 160 1e-040
gb|CO514307.1|CO514307 s13dSG76H0900076_157276 Glandular tr... 160 1e-040
gb|CO512439.1|CO512439 s13dSG80H0800064_114392 Glandular tr... 144 1e-035
gb|L06967.1|ALFUBIQUIT Medicago sativa ubiquitin carrier pr... 144 1e-035
gb|CO516470.1|CO516470 s13dSG28C0900068_446268 Glandular tr... 142 7e-035
gb|CO512021.1|CO512021 s13dSG07H0700064_103942 Glandular tr... 137 2e-033
gb|CO512178.1|CO512178 s13dSG34H1100092_108676 Glandular tr... 134 1e-032
gb|CO514418.1|CO514418 s13dSG36D0500044_327496 Glandular tr... 132 5e-032
gb|AW698741.1|AW698741 r315 non-glandular-haired subtracted... 107 1e-030
gb|CO516360.1|CO516360 s13dSG99F0200015_446048 Glandular tr... 122 6e-029
gb|CO517244.1|CO517244 s13dSG32E0500039_486084 Glandular tr... 100 2e-022
>gb|AW698442.1|AW698442 G296 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 345
Score = 246 bits (532), Expect = 2e-066
Identities = 101/105 (96%), Positives = 103/105 (98%)
Frame = +1 / -2
Query: 55 MANSNLPRRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPAQSPYEGGVFKLELFL 234
MANSNLPRRIIKETQR LSEPAPGISASPSE+NMRYFNVMILGP QSPYEGGVFKL+LFL
Sbjct: 317 MANSNLPRRIIKETQRXLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLDLFL 138
Query: 235 PEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRT 369
PEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRT
Sbjct: 137 PEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRT 3
Score = 196 bits (422), Expect = 4e-051
Identities = 88/106 (83%), Positives = 94/106 (88%)
Frame = -2 / +1
Query: 371 TVRI*RAGLHLSLRMSRHILPSLSMFGW*ILVRNLTFGAAIGYSSGKNSSSLKTPPS*GD 192
TVRI*RAGLHLS R+SRHILPSLSM G I V+NLTFGAAIGYSSGKN SSLKTPPS*GD
Sbjct: 1 TVRI*RAGLHLSFRISRHILPSLSMLG*YIFVKNLTFGAAIGYSSGKNRSSLKTPPS*GD 180
Query: 191 CAGPRIMTLKYRMFSSDGDALIPGAGSLRSRCVSLMIRRGRLLLAM 54
GPRI+TLKYR+ SS+GDALIPGAGSL RCVSLMI RGRLLLA+
Sbjct: 181 *VGPRIITLKYRILSSEGDALIPGAGSLSXRCVSLMILRGRLLLAI 318
Score = 128 bits (274), Expect = 9e-031
Identities = 53/102 (51%), Positives = 68/102 (66%)
Frame = -1 / +3
Query: 360 LKSGTPFVLKNVEAYPTKLVNVRMVNFGEKPNFRGSHWIFLRQE*LKLENSTFIG*LCGA 181
LK GTP + +N++ YP KLVNVRM+ F ++P+ SHWIF RQ+*+ LENS FI L A
Sbjct: 12 LKGGTPLIFQNIKTYPAKLVNVRMIYFCQEPDLWSSHWIFFRQK*V*LENSPFIRRLSWA 191
Query: 180 KDHDIEVPHVLFXXXXXXXXRWFAEEPLRLLDDSAREVAVGH 55
KDH IE+PH++F RW E L LDDS+R+V V H
Sbjct: 192 KDHHIEIPHIVFRRGCTNSRRWLTEXTLCFLDDSSRKVTVSH 317
Score = 111 bits (236), Expect = 2e-025
Identities = 54/105 (51%), Positives = 70/105 (66%)
Frame = +3 / -1
Query: 54 HGQQQPPAPNHQGDAAAPQRTSAGD*RVAVGREHAVLQCHDPWPRTVTL*RWSFQA*AIL 233
+G Q P + NHQG+ A Q SA +* + GR++AV QC DPWP +V+L*R SFQA I
Sbjct: 318 NG*Q*PSSKNHQGNTAXAQ*ASAWN*CIPFGRQYAVFQCDDPWPNSVSL*RGSFQARPIF 139
Query: 234 A*GISNGCPES*VSHQNLPSEH*QAW*DMPRHS*GQMESRSSDTY 368
A* ISNGC + V +N+ S H*QAW DM +S +ESR SD++
Sbjct: 138 A*RISNGCSKGQVLDKNISS*H*QAWQDMS*YSER*VESRPSDSH 4
>gb|AW698617.1|AW698617 g513 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 344
Score = 234 bits (506), Expect = 9e-063
Identities = 97/100 (97%), Positives = 97/100 (97%)
Frame = +1 / -1
Query: 70 LPRRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPAQSPYEGGVFKLELFLPEEYP 249
LPRRIIKETQRLLSEPAPGI ASPSE NMRYFNVMILGP QSPYEGGVFKLELFLPEEYP
Sbjct: 302 LPRRIIKETQRLLSEPAPGIRASPSEVNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYP 123
Query: 250 MAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRT 369
MAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRT
Sbjct: 122 MAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRT 3
Score = 186 bits (401), Expect = 3e-048
Identities = 83/101 (82%), Positives = 89/101 (88%)
Frame = -2 / +1
Query: 371 TVRI*RAGLHLSLRMSRHILPSLSMFGW*ILVRNLTFGAAIGYSSGKNSSSLKTPPS*GD 192
TVRI*RAGLHLS R+SRHILPSLSM G I V+NLTFGAAIGYSSGKNSSSLKTPPS*GD
Sbjct: 1 TVRI*RAGLHLSFRISRHILPSLSMLG*YIFVKNLTFGAAIGYSSGKNSSSLKTPPS*GD 180
Query: 191 CAGPRIMTLKYRMFSSDGDALIPGAGSLRSRCVSLMIRRGR 69
GPRI+TLKYR+ +S+GDA IPGAGSL RCVSLMI RGR
Sbjct: 181 *VGPRIITLKYRILTSEGDARIPGAGSLSKRCVSLMILRGR 303
Score = 116 bits (248), Expect = 4e-027
Identities = 50/98 (51%), Positives = 63/98 (64%)
Frame = -1 / +3
Query: 360 LKSGTPFVLKNVEAYPTKLVNVRMVNFGEKPNFRGSHWIFLRQE*LKLENSTFIG*LCGA 181
LK TP + +N++ YP KLVNVRM+ F ++ N SHWIF RQ+* LENS FI L A
Sbjct: 12 LKGRTPLIFQNIKTYPAKLVNVRMIYFCQESNLWSSHWIFFRQK*F*LENSPFIRRLSWA 191
Query: 180 KDHDIEVPHVLFXXXXXXXXRWFAEEPLRLLDDSAREV 67
KDH IE+PH+ F RW E+ L LDDS+R+V
Sbjct: 192 KDHHIEIPHIDFRRGCTNSRRWLTEQTLCFLDDSSRKV 305
Score = 101 bits (216), Expect = 9e-023
Identities = 49/100 (49%), Positives = 65/100 (65%)
Frame = +3 / -3
Query: 69 PPAPNHQGDAAAPQRTSAGD*RVAVGREHAVLQCHDPWPRTVTL*RWSFQA*AILA*GIS 248
P + NHQG+ A Q SA + + G ++AV QC DPWP +V+L*R SFQA I A* IS
Sbjct: 303 PSSKNHQGNTAFAQ*ASAWNSCIPFGSQYAVFQCDDPWPNSVSL*RGSFQARTIFA*RIS 124
Query: 249 NGCPES*VSHQNLPSEH*QAW*DMPRHS*GQMESRSSDTY 368
NGC + *+ +N+ S H*QAW DM +S +ES SD++
Sbjct: 123 NGCSKG*ILDKNISS*H*QAWQDMS*YSER*VESCPSDSH 4
>gb|CO514742.1|CO514742 s13dSG55F0200027_328144 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 526
Score = 176 bits (379), Expect = 3e-045
Identities = 72/145 (49%), Positives = 97/145 (66%)
Frame = +1 / +2
Query: 76 RRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPAQSPYEGGVFKLELFLPEEYPMA 255
+RI+KE + L +P SA P E+M ++ I+GPA SPY GGVF + + P +YP
Sbjct: 83 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPADSPYAGGVFLVSIHFPPDYPFK 262
Query: 256 APKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNI 435
PKV F TK++HPNI+ G ICLDILK++WSPAL I VLLSI +L++ PNPDDPL I
Sbjct: 263 PPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLVTDPNPDDPLVPEI 442
Query: 436 AKHWKSNEAEAFETAKEWTRLYASG 510
A +K++ + TA+ WT+ YA G
Sbjct: 443 AHMYKTDRTKYEATARSWTQKYAMG 517
Score = 105 bits (224), Expect = 7e-024
Identities = 63/153 (41%), Positives = 79/153 (51%)
Frame = -2 / -2
Query: 509 PLAYRRVHSFAVSKASASFDFQCFAMLSERGSSGFGALRSA*MLSRTVRI*RAGLHLSLR 330
P+AY V AV+ A+ GSSG G++ + + T+ + AGLH SLR
Sbjct: 516 PMAYFCVQLRAVASYLVLSVLYICAISGTSGSSGLGSVTNEQIDKSTLEMVSAGLHCSLR 337
Query: 329 MSRHILPSLSMFGW*ILVRNLTFGAAIGYSSGKNSSSLKTPPS*GDCAGPRIMTLKYRMF 150
MSR ILP L MFGW LV N TFG G S GK + TPP+*G AGP I+ + M
Sbjct: 336 MSRQILPLLLMFGWNTLVLNATFGGLNG*SGGK*IETKNTPPA*GLSAGPMIVACQ*NMS 157
Query: 149 SSDGDALIPGAGSLRSRCVSLMIRRGRLLLAME 51
S+ G AL GS SL IR +L + E
Sbjct: 156 SATGPALHDVGGSFCKSLSSLRIRFDAMLKSKE 58
>gb|CO512153.1|CO512153 s13dSG34E0900067_108626 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 582
Score = 173 bits (372), Expect = 3e-044
Identities = 71/143 (49%), Positives = 98/143 (68%)
Frame = +1 / +1
Query: 76 RRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPAQSPYEGGVFKLELFLPEEYPMA 255
+RI KE + L +P SA P E++ ++ I+GPA SPY GGVF + + P +YP
Sbjct: 94 KRITKELKDLQKDPPVSCSAGPVGEDLFHWQATIMGPADSPYAGGVFLVTIHFPPDYPSK 273
Query: 256 APKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNI 435
PKV F TK++HPNI+ G ICLDILK++WSPAL + VLLSI +LL+ PNPDDPL +I
Sbjct: 274 PPKVSFRTKVFHPNINSNGSICLDILKEQWSPALTVSKVLLSICSLLTDPNPDDPLVPDI 453
Query: 436 AKHWKSNEAEAFETAKEWTRLYA 504
A +K+++A+ TA+ WT+ YA
Sbjct: 454 AHMYKTDKAKYETTARSWTQKYA 522
Score = 99.6 bits (211), Expect = 5e-022
Identities = 60/143 (41%), Positives = 73/143 (51%)
Frame = -2 / -2
Query: 506 LAYRRVHSFAVSKASASFDFQCFAMLSERGSSGFGALRSA*MLSRTVRI*RAGLHLSLRM 327
+AY V AV A +A+ +GSSGFG++ + SRT RAGLH S RM
Sbjct: 524 IAYF*VQDRAVVSYLALSVLYMWAISGTKGSSGFGSVSREQIDSRTFETVRAGLHCSFRM 345
Query: 326 SRHILPSLSMFGW*ILVRNLTFGAAIGYSSGKNSSSLKTPPS*GDCAGPRIMTLKYRMFS 147
SR ILP L M GW VR TFG G S GK + TPP+*G AGP I+ + S
Sbjct: 344 SRQILPLLLMLGWNTFVRKETFGGLDG*SGGK*MVTRNTPPA*GLSAGPMIVACQ*NRSS 165
Query: 146 SDGDALIPGAGSLRSRCVSLMIR 78
G AL GS SL++R
Sbjct: 164 PTGPALQDTGGSFCKSFNSLVMR 96
>gb|CO514801.1|CO514801 s13dSG59D0300030_328262 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 586
Score = 173 bits (372), Expect = 3e-044
Identities = 72/145 (49%), Positives = 96/145 (66%)
Frame = +1 / +1
Query: 76 RRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPAQSPYEGGVFKLELFLPEEYPMA 255
+RI+KE + L +P SA P E+M ++ I+GPA SPY GGVF + + P +YP
Sbjct: 94 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPADSPYTGGVFLVSIHFPPDYPFK 273
Query: 256 APKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNI 435
PK F TK++HPNI+ G ICLDILK++WSPAL I LLSI +LL+ PNPDDPL I
Sbjct: 274 PPKGAFRTKVFHPNINSNGSICLDILKEQWSPALTISKGLLSICSLLTDPNPDDPLVPEI 453
Query: 436 AKHWKSNEAEAFETAKEWTRLYASG 510
A +K++ A+ TA+ WT+ YA G
Sbjct: 454 AHMYKTDRAKYEATARSWTQKYAMG 528
Score = 100 bits (213), Expect = 2e-022
Identities = 58/147 (39%), Positives = 74/147 (50%)
Frame = -2 / -3
Query: 518 YNAPLAYRRVHSFAVSKASASFDFQCFAMLSERGSSGFGALRSA*MLSRTVRI*RAGLHL 339
++ P+AY V AV+ A A+ GSSG G++ + + R + + AGLH
Sbjct: 536 HDHPMAYFCVQLRAVASYLALSVLYMCAISGTSGSSGLGSVNNEQIDKRPLEMVNAGLHC 357
Query: 338 SLRMSRHILPSLSMFGW*ILVRNLTFGAAIGYSSGKNSSSLKTPPS*GDCAGPRIMTLKY 159
S RMSRHILP L MFGW LV FG G S GK + TPP G AGP I+ ++
Sbjct: 356 SFRMSRHILPLLLMFGWNTLVLKAPFGGLKG*SGGK*IETRNTPPVYGLSAGPMIVACQW 177
Query: 158 RMFSSDGDALIPGAGSLRSRCVSLMIR 78
M S+ G AL GS S IR
Sbjct: 176 NMSSATGPALHEVGGSFCKSLSSFKIR 96
>gb|CO511830.1|CO511830 s13dSG02E0400035_103560 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 519
Score = 166 bits (358), Expect = 2e-042
Identities = 69/148 (46%), Positives = 94/148 (63%)
Frame = +1 / +1
Query: 49 GSMANSNLPRRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPAQSPYEGGVFKLEL 228
G+ +RI KE + L +P SA P ++M ++ I+GP SP+ GGVF +++
Sbjct: 76 GTSVTEMASKRINKELKDLQKDPPASCSAGPVADDMFHWQATIMGPGDSPFAGGVFLVQI 255
Query: 229 FLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPN 408
P +YP PKV F TK++HPNI+ G ICLDILK++WSPA I VLLSI +LL+ PN
Sbjct: 256 HFPPDYPFKPPKVSFRTKVFHPNINSNGSICLDILKEQWSPAFTISKVLLSICSLLTDPN 435
Query: 409 PDDPLSDNIAKHWKSNEAEAFETAKEWT 492
PDDPL IA +KS +A+ TA+ WT
Sbjct: 436 PDDPLVPEIAHMYKSXQAKYGATARSWT 519
Score = 98.2 bits (208), Expect = 1e-021
Identities = 57/148 (38%), Positives = 76/148 (51%)
Frame = -2 / -2
Query: 491 VHSFAVSKASASFDFQCFAMLSERGSSGFGALRSA*MLSRTVRI*RAGLHLSLRMSRHIL 312
VH AV+ A A+ RGSSG G++ M S T+ I +AGLH S RMSR +L
Sbjct: 518 VHDRAVAPYLAXSLLYI*AISGTRGSSGLGSVNREQMDSSTLDIVKAGLHCSFRMSRQML 339
Query: 311 PSLSMFGW*ILVRNLTFGAAIGYSSGKNSSSLKTPPS*GDCAGPRIMTLKYRMFSSDGDA 132
P L + GW VRN T G G S GK + TPP+ G GP I+ ++ + S+ G A
Sbjct: 338 PLLLILGWKTFVRNETLGGLKG*SGGK*ICTRNTPPANGLSPGPIIVACQWNISSATGPA 159
Query: 131 LIPGAGSLRSRCVSLMIRRGRLLLAMEP 48
L GS SL+IR + + + P
Sbjct: 158 LQEAGGSFCKSFNSLLIRFEAISVTLVP 75
>gb|CO515876.1|CO515876 s13dSG91B0900075_421291 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 518
Score = 165 bits (355), Expect = 6e-042
Identities = 70/145 (48%), Positives = 95/145 (65%)
Frame = +1 / +3
Query: 58 ANSNLPRRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPAQSPYEGGVFKLELFLP 237
+NS +RI+KE + L +P SA P E+M ++ I+GP SPY GGVF + + P
Sbjct: 84 SNSMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFP 263
Query: 238 EEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDD 417
+YP PKV F TK++HPNI+ G ICLDILK++WSPAL I VLLSI +LL+ PNPDD
Sbjct: 264 PDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDD 443
Query: 418 PLSDNIAKHWKSNEAEAFETAKEWT 492
PL IA +K++ ++ TA+ T
Sbjct: 444 PLVPEIAHMYKTDRSKYETTARSRT 518
Score = 95.5 bits (202), Expect = 8e-021
Identities = 52/120 (43%), Positives = 68/120 (56%)
Frame = -2 / -2
Query: 437 AMLSERGSSGFGALRSA*MLSRTVRI*RAGLHLSLRMSRHILPSLSMFGW*ILVRNLTFG 258
A+ +GSSGFG++ M + T+ I +AGLH S ++SR +LP L +FGW LV N T G
Sbjct: 463 AISGTKGSSGFGSVNREQMDNSTLDIVKAGLHCSFKISRQMLPLLLIFGWKTLVLNATLG 284
Query: 257 AAIGYSSGKNSSSLKTPPS*GDCAGPRIMTLKYRMFSSDGDALIPGAGSLRSRCVSLMIR 78
G S GK + KTPP+*G GP I+ + M S+ G AL GS SL IR
Sbjct: 283 GLNG*SGGK*MVTRKTPPA*GLSGGPIIVACQ*NMSSATGPALQEVGGSF*RSLSSLRIR 104
>gb|CO512082.1|CO512082 s13dSG18F0600047_108484 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 587
Score = 160 bits (345), Expect = 1e-040
Identities = 66/127 (51%), Positives = 87/127 (68%)
Frame = +1 / +3
Query: 76 RRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPAQSPYEGGVFKLELFLPEEYPMA 255
+RI+KE + L +P SA P E+M ++ I+GPA SPY GGVF + + P +YP
Sbjct: 207 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPADSPYAGGVFLVSIHFPPDYPFK 386
Query: 256 APKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNI 435
PKV F TK++HPNI+ G ICLDILK++WSPAL I VLLSI +LL+ PNPDDPL I
Sbjct: 387 PPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEI 566
Query: 436 AKHWKSN 456
A +K++
Sbjct: 567 AHMYKTD 587
Score = 102 bits (218), Expect = 5e-023
Identities = 57/129 (44%), Positives = 71/129 (55%)
Frame = -2 / -2
Query: 437 AMLSERGSSGFGALRSA*MLSRTVRI*RAGLHLSLRMSRHILPSLSMFGW*ILVRNLTFG 258
A+ GSSG G++ + + T+ + AGLH SLRMSR ILP L MFGW LV N TFG
Sbjct: 568 AISGTSGSSGLGSVNNEQIDKSTLEMVSAGLHCSLRMSRQILPLLLMFGWNTLVLNATFG 389
Query: 257 AAIGYSSGKNSSSLKTPPS*GDCAGPRIMTLKYRMFSSDGDALIPGAGSLRSRCVSLMIR 78
G S GK + TPP+*G AGP I+ + M S+ G AL GS SL IR
Sbjct: 388 GLNG*SGGK*IETKNTPPA*GLSAGPMIVACQ*NMSSATGPALHDVGGSFCKSLSSLRIR 209
Query: 77 RGRLLLAME 51
+L + E
Sbjct: 208 FDAMLKSKE 182
>gb|CO514307.1|CO514307 s13dSG76H0900076_157276 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 490
Score = 160 bits (345), Expect = 1e-040
Identities = 66/127 (51%), Positives = 87/127 (68%)
Frame = +1 / +1
Query: 76 RRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPAQSPYEGGVFKLELFLPEEYPMA 255
+RI+KE + L +P SA P E+M ++ I+GPA SPY GGVF + + P +YP
Sbjct: 109 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPADSPYAGGVFLVSIHFPPDYPFK 288
Query: 256 APKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNI 435
PKV F TK++HPNI+ G ICLDILK++WSPAL I VLLSI +LL+ PNPDDPL I
Sbjct: 289 PPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEI 468
Query: 436 AKHWKSN 456
A +K++
Sbjct: 469 AHMYKTD 489
Score = 101 bits (215), Expect = 1e-022
Identities = 56/125 (44%), Positives = 69/125 (55%)
Frame = -2 / -3
Query: 437 AMLSERGSSGFGALRSA*MLSRTVRI*RAGLHLSLRMSRHILPSLSMFGW*ILVRNLTFG 258
A+ GSSG G++ + + T+ + AGLH SLRMSR ILP L MFGW LV N TFG
Sbjct: 470 AISGTSGSSGLGSVNNEQIDKSTLEMVSAGLHCSLRMSRQILPLLLMFGWNTLVLNATFG 291
Query: 257 AAIGYSSGKNSSSLKTPPS*GDCAGPRIMTLKYRMFSSDGDALIPGAGSLRSRCVSLMIR 78
G S GK + TPP+*G AGP I+ + M S+ G AL GS SL IR
Sbjct: 290 GLNG*SGGK*IETKNTPPA*GLSAGPMIVACQ*NMSSATGPALHDVGGSFCKSLSSLRIR 111
Query: 77 RGRLL 63
+L
Sbjct: 110 FDAML 96
>gb|CO512439.1|CO512439 s13dSG80H0800064_114392 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 567
Score = 144 bits (310), Expect = 1e-035
Identities = 55/137 (40%), Positives = 88/137 (64%)
Frame = +1 / +3
Query: 76 RRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPAQSPYEGGVFKLELFLPEEYPMA 255
+R++++ +RL +P GIS +P + N+ +N +I GP +P++GG FKL L E+YP
Sbjct: 93 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNK 272
Query: 256 APKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNI 435
P VRF+++++HPNI G ICLDIL+++WSP + +L SIQ+LL PNP+ P +
Sbjct: 273 PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEA 452
Query: 436 AKHWKSNEAEAFETAKE 486
A+ + N+ E +E
Sbjct: 453 ARMFSENKREYNRRVRE 503
>gb|L06967.1|ALFUBIQUIT Medicago sativa ubiquitin carrier protein mRNA, complete cds
Length = 910
Score = 144 bits (310), Expect = 1e-035
Identities = 55/137 (40%), Positives = 88/137 (64%)
Frame = +1 / +2
Query: 76 RRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPAQSPYEGGVFKLELFLPEEYPMA 255
+R++++ +RL +P GIS +P + N+ +N +I GP +P++GG FKL L E+YP
Sbjct: 143 KRLMRDFKRLQHDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNK 322
Query: 256 APKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSDNI 435
P VRF+++++HPNI G ICLDIL+++WSP + +L SIQ+LL PNP+ P +
Sbjct: 323 PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEA 502
Query: 436 AKHWKSNEAEAFETAKE 486
A+ + N+ E +E
Sbjct: 503 ARMFSENKREYNRRVRE 553
>gb|CO516470.1|CO516470 s13dSG28C0900068_446268 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 418
Score = 142 bits (304), Expect = 7e-035
Identities = 58/111 (52%), Positives = 76/111 (68%)
Frame = +1 / +3
Query: 76 RRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPAQSPYEGGVFKLELFLPEEYPMA 255
+RI+KE + L +P SA P E+M ++ I+GPA SPY GGVF + + P +YP
Sbjct: 84 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPADSPYAGGVFLVSIHFPPDYPFK 263
Query: 256 APKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPN 408
PKV F TK++HPNI+ G ICLDILK++WSPAL I VLLSI +LL+ PN
Sbjct: 264 PPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPN 416
Score = 95.9 bits (203), Expect = 6e-021
Identities = 53/120 (44%), Positives = 66/120 (55%)
Frame = -2 / -1
Query: 410 GFGALRSA*MLSRTVRI*RAGLHLSLRMSRHILPSLSMFGW*ILVRNLTFGAAIGYSSGK 231
G G++ + + T+ + AGLH SLRMSR ILP L MFGW LV N TFG G S GK
Sbjct: 418 GLGSVNNEQIDKSTLEMVSAGLHCSLRMSRQILPLLLMFGWNTLVLNATFGGLNG*SGGK 239
Query: 230 NSSSLKTPPS*GDCAGPRIMTLKYRMFSSDGDALIPGAGSLRSRCVSLMIRRGRLLLAME 51
+ TPP+*G AGP I+ + M S+ G AL GS SL IR +L + E
Sbjct: 238 *IETKNTPPA*GLSAGPMIVACQ*NMSSATGPALHDVGGSFCKSLSSLRIRFDAMLKSKE 59
>gb|CO512021.1|CO512021 s13dSG07H0700064_103942 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 584
Score = 137 bits (294), Expect = 2e-033
Identities = 51/115 (44%), Positives = 79/115 (68%)
Frame = +1 / +2
Query: 76 RRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPAQSPYEGGVFKLELFLPEEYPMA 255
+R++++ +RL +P GIS +P + N+ +N +I GP +P++GG FKL L E+YP
Sbjct: 230 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNK 409
Query: 256 APKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDP 420
P VRF+++++HPNI G ICLDIL+++WSP + +L SIQ+LL PNP+ P
Sbjct: 410 PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSP 574
>gb|CO512178.1|CO512178 s13dSG34H1100092_108676 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 585
Score = 134 bits (287), Expect = 1e-032
Identities = 50/115 (43%), Positives = 78/115 (67%)
Frame = +1 / +2
Query: 76 RRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPAQSPYEGGVFKLELFLPEEYPMA 255
+R++++ +RL +P GIS +P + N+ +N +I GP +P++GG FKL L E+YP
Sbjct: 230 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNK 409
Query: 256 APKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDP 420
P VRF+++++HPNI G ICLDIL+++WSP + +L SIQ+LL P P+ P
Sbjct: 410 PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPXPNSP 574
>gb|CO514418.1|CO514418 s13dSG36D0500044_327496 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 589
Score = 132 bits (283), Expect = 5e-032
Identities = 50/115 (43%), Positives = 77/115 (66%)
Frame = +1 / +2
Query: 76 RRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPAQSPYEGGVFKLELFLPEEYPMA 255
+R++++ +RL +P GIS +P + N+ +N +I GP +P+ GG FKL L E+YP
Sbjct: 230 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWNGGTFKLTLQFTEDYPNK 409
Query: 256 APKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDP 420
P VRF+++++HPNI G ICLDIL+++WSP + +L SIQ+LL PN + P
Sbjct: 410 PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNXNSP 574
>gb|AW698741.1|AW698741 r315 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 335
Score = 107 bits (229), Expect(2) = 1e-030
Identities = 41/76 (53%), Positives = 53/76 (69%)
Frame = +1 / -3
Query: 130 SASPSEENMRYFNVMILGPAQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL 309
SA P E+M ++ I+GPA SPY GGVF + + P +YP PKV TK++HPNI+
Sbjct: 315 SAGPVAEDMFHWQATIMGPADSPYAGGVFLVSIHFPPDYPFKPPKVAXRTKVFHPNINSN 136
Query: 310 GRICLDILKDKWSPAL 357
G ICLDILK++WSPAL
Sbjct: 135 GSICLDILKEQWSPAL 88
Score = 40.0 bits (81), Expect(2) = 1e-030
Identities = 18/30 (60%), Positives = 20/30 (66%)
Frame = +1 / -2
Query: 349 PALQIRTVLLSIQALLSAPNPDDPLSDNIA 438
P I VLLSI +LL+ PNPDDPL IA
Sbjct: 97 PCTDISKVLLSICSLLTDPNPDDPLVPEIA 8
>gb|CO516360.1|CO516360 s13dSG99F0200015_446048 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 396
Score = 122 bits (261), Expect = 6e-029
Identities = 45/105 (42%), Positives = 72/105 (68%)
Frame = +1 / +3
Query: 76 RRIIKETQRLLSEPAPGISASPSEENMRYFNVMILGPAQSPYEGGVFKLELFLPEEYPMA 255
+R++++ +RL +P GIS +P + N+ +N +I GP +P++GG FKL L E+YP
Sbjct: 81 KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNK 260
Query: 256 APKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQA 390
P VRF+++++HPNI G ICLDIL+++WSP + +L SIQ+
Sbjct: 261 PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQS 395
>gb|CO517244.1|CO517244 s13dSG32E0500039_486084 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 556
Score = 100 bits (214), Expect = 2e-022
Identities = 41/94 (43%), Positives = 58/94 (61%)
Frame = +1 / -3
Query: 205 GGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSI 384
GG FKL L E+YP P VRF+++++HPNI G ICLDIL++ WSP + +L SI
Sbjct: 554 GGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQN*WSPIYDVAAILTSI 375
Query: 385 QALLSAPNPDDPLSDNIAKHWKSNEAEAFETAKE 486
Q+LL PNP+ P + A+ + N+ E +E
Sbjct: 374 QSLLCDPNPNSPANSEAARMFSENKREYNRRVRE 273
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,406,138
Number of Sequences: 7669
Number of extensions: 109045
Number of successful extensions: 1137
Number of sequences better than 1.0e-020: 23
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 214941040
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)