TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3390529.2.1
(407 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO515980.1|CO515980 s13dSG61E0700055_445288 Glandular tr... 134 5e-033
gb|M91079.1|ALFMSCHI1 Medicago sativa (cultivar Iroquois) c... 134 5e-033
gb|M91080.1|ALFMSCHI2 Medicago sativa (cultivar Iroquois) c... 133 1e-032
gb|CO516149.1|CO516149 s13dSG67A0200005_445626 Glandular tr... 113 1e-026
>gb|CO515980.1|CO515980 s13dSG61E0700055_445288 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 437
Score = 134 bits (288), Expect = 5e-033
Identities = 57/98 (58%), Positives = 71/98 (72%)
Frame = +1 / +1
Query: 112 PGSAGSHFLGGAGVRGLEIGGNFIKFTAIGVYLEDAAVPALAKKWGGKTADELASDAAFF 291
P + S+FLGGAG RGL I GNFIKFTAIGVYLED AV +LA KW GK+++EL F+
Sbjct: 121 PVTGKSYFLGGAGERGLTIEGNFIKFTAIGVYLEDIAVASLAAKWKGKSSEELLETLDFY 300
Query: 292 RDVVTGDFEKFTRVTMILPLTGEQYAEKVTENCVAFWK 405
RD+++G FEK R + I L+G +Y+ KV ENCVA K
Sbjct: 301 RDIISGPFEKLIRGSKIRELSGPEYSRKVMENCVAHLK 414
>gb|M91079.1|ALFMSCHI1 Medicago sativa (cultivar Iroquois) chalcone isomerase (MsCHI-1)
mRNA, complete cds
Length = 950
Score = 134 bits (288), Expect = 5e-033
Identities = 57/98 (58%), Positives = 71/98 (72%)
Frame = +1 / +2
Query: 112 PGSAGSHFLGGAGVRGLEIGGNFIKFTAIGVYLEDAAVPALAKKWGGKTADELASDAAFF 291
P + S+FLGGAG RGL I GNFIKFTAIGVYLED AV +LA KW GK+++EL F+
Sbjct: 104 PVTGKSYFLGGAGERGLTIEGNFIKFTAIGVYLEDIAVASLAAKWKGKSSEELLETLDFY 283
Query: 292 RDVVTGDFEKFTRVTMILPLTGEQYAEKVTENCVAFWK 405
RD+++G FEK R + I L+G +Y+ KV ENCVA K
Sbjct: 284 RDIISGPFEKLIRGSKIRELSGPEYSRKVMENCVAHLK 397
>gb|M91080.1|ALFMSCHI2 Medicago sativa (cultivar Iroquois) chalcone isomerase (MsCHI-2)
mRNA, 3'end
Length = 836
Score = 133 bits (285), Expect = 1e-032
Identities = 56/93 (60%), Positives = 69/93 (74%)
Frame = +1 / +2
Query: 127 SHFLGGAGVRGLEIGGNFIKFTAIGVYLEDAAVPALAKKWGGKTADELASDAAFFRDVVT 306
S+FLGGAG RGL I GNFIKFTAIGVYLED AV +LA KW GK+++EL F+RD+++
Sbjct: 5 SYFLGGAGERGLTIEGNFIKFTAIGVYLEDIAVASLAAKWKGKSSEELLETLDFYRDIIS 184
Query: 307 GDFEKFTRVTMILPLTGEQYAEKVTENCVAFWK 405
G FEK R + I L+G +Y+ KV ENCVA K
Sbjct: 185 GPFEKLIRGSKIRELSGPEYSRKVMENCVAHLK 283
>gb|CO516149.1|CO516149 s13dSG67A0200005_445626 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 367
Score = 113 bits (241), Expect = 1e-026
Identities = 48/82 (58%), Positives = 60/82 (73%)
Frame = +1 / +1
Query: 112 PGSAGSHFLGGAGVRGLEIGGNFIKFTAIGVYLEDAAVPALAKKWGGKTADELASDAAFF 291
P + S+FLGGAG RGL I GNFIKFTAIGVYLED AV +LA KW GK+++EL F+
Sbjct: 118 PVTGKSYFLGGAGERGLTIEGNFIKFTAIGVYLEDIAVASLAAKWKGKSSEELLETLDFY 297
Query: 292 RDVVTGDFEKFTRVTMILPLTG 357
RD+++G FEK R + I L+G
Sbjct: 298 RDIISGPFEKLIRGSKIRELSG 363
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,649,669
Number of Sequences: 7669
Number of extensions: 11921
Number of successful extensions: 1117
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 40
effective length of database: 941,808
effective search space used: 89471760
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)