TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3203838.2.1
(534 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF083333.1|AF083333 Medicago sativa cinnamyl-alcohol deh... 132 4e-032
gb|L46856.1|ALFCAD1A Medicago sativa cinnamyl-alcohol dehyd... 132 4e-032
gb|AF083332.1|AF083332 Medicago sativa cinnamyl-alcohol deh... 103 2e-023
emb|Z19573.1|MSCIALDHA M.sativa encoding cinnamyl alcohol d... 103 2e-023
gb|L46857.1|ALFCAD1B Medicago sativa cinnamyl-alcohol dehyd... 103 2e-023
>gb|AF083333.1|AF083333 Medicago sativa cinnamyl-alcohol dehydrogenase (MsaCad1) mRNA,
complete cds
Length = 1390
Score = 132 bits (282), Expect = 4e-032
Identities = 52/95 (54%), Positives = 71/95 (74%)
Frame = -2 / +3
Query: 503 LALLKPQGKMILLGLPEKPLQISAFSLVTGGKTLAGSCMGSIRDTQEMMDFAAKHGLTAD 324
L LLK GK++ +GLP KPL++S F LV G K + GS +G +++TQEM+DF KH +TAD
Sbjct: 891 LGLLKLNGKLVTVGLPSKPLELSVFPLVAGRKLIGGSNIGGMKETQEMLDFCGKHNITAD 1070
Query: 323 IELIGTEEVNEAMERLAKGEVRYRFVIDIGNTLNA 219
IELI E+N AMERL K +V+YRFVID+ N+ ++
Sbjct: 1071 IELIKMHEINTAMERLHKADVKYRFVIDVANSFSS 1175
>gb|L46856.1|ALFCAD1A Medicago sativa cinnamyl-alcohol dehydrogenase (cad1) mRNA, complete
cds
Length = 1390
Score = 132 bits (282), Expect = 4e-032
Identities = 52/95 (54%), Positives = 71/95 (74%)
Frame = -2 / +3
Query: 503 LALLKPQGKMILLGLPEKPLQISAFSLVTGGKTLAGSCMGSIRDTQEMMDFAAKHGLTAD 324
L LLK GK++ +GLP KPL++S F LV G K + GS +G +++TQEM+DF KH +TAD
Sbjct: 891 LGLLKLNGKLVTVGLPSKPLELSVFPLVAGRKLIGGSNIGGMKETQEMLDFCGKHNITAD 1070
Query: 323 IELIGTEEVNEAMERLAKGEVRYRFVIDIGNTLNA 219
IELI E+N AMERL K +V+YRFVID+ N+ ++
Sbjct: 1071 IELIKMHEINTAMERLHKADVKYRFVIDVANSFSS 1175
>gb|AF083332.1|AF083332 Medicago sativa cinnamyl-alcohol dehydrogenase (MsaCad2) mRNA,
complete cds
Length = 1324
Score = 103 bits (220), Expect = 2e-023
Identities = 41/90 (45%), Positives = 66/90 (73%)
Frame = -2 / +3
Query: 506 YLALLKPQGKMILLGLPEKPLQISAFSLVTGGKTLAGSCMGSIRDTQEMMDFAAKHGLTA 327
YL+LLK GK+IL+G+ PLQ ++ G K++ GS +GS+++T+EM++F + GLT+
Sbjct: 840 YLSLLKIDGKLILMGVINTPLQFVTPMVMLGRKSITGSFVGSVKETEEMLEFWKEKGLTS 1019
Query: 326 DIELIGTEEVNEAMERLAKGEVRYRFVIDI 237
IE++ + +N+A ERL K +VRYRFV+D+
Sbjct: 1020 MIEIVTMDYINKAFERLEKNDVRYRFVVDV 1109
>emb|Z19573.1|MSCIALDHA M.sativa encoding cinnamyl alcohol dehydrogenase
Length = 1321
Score = 103 bits (220), Expect = 2e-023
Identities = 41/90 (45%), Positives = 66/90 (73%)
Frame = -2 / +3
Query: 506 YLALLKPQGKMILLGLPEKPLQISAFSLVTGGKTLAGSCMGSIRDTQEMMDFAAKHGLTA 327
YL+LLK GK+IL+G+ PLQ ++ G K++ GS +GS+++T+EM++F + GLT+
Sbjct: 864 YLSLLKIDGKLILMGVINTPLQFVTPMVMLGRKSITGSFVGSVKETEEMLEFWKEKGLTS 1043
Query: 326 DIELIGTEEVNEAMERLAKGEVRYRFVIDI 237
IE++ + +N+A ERL K +VRYRFV+D+
Sbjct: 1044 MIEIVTMDYINKAFERLEKNDVRYRFVVDV 1133
>gb|L46857.1|ALFCAD1B Medicago sativa cinnamyl-alcohol dehydrogenase (cad1) mRNA, partial
cds
Length = 1201
Score = 103 bits (220), Expect = 2e-023
Identities = 41/90 (45%), Positives = 66/90 (73%)
Frame = -2 / +3
Query: 506 YLALLKPQGKMILLGLPEKPLQISAFSLVTGGKTLAGSCMGSIRDTQEMMDFAAKHGLTA 327
YL+LLK GK+IL+G+ PLQ ++ G K++ GS +GS+++T+EM++F + GLT+
Sbjct: 717 YLSLLKIDGKLILMGVINTPLQFVTPMVMLGRKSITGSFVGSVKETEEMLEFWKEKGLTS 896
Query: 326 DIELIGTEEVNEAMERLAKGEVRYRFVIDI 237
IE++ + +N+A ERL K +VRYRFV+D+
Sbjct: 897 MIEIVTMDYINKAFERLEKNDVRYRFVVDV 986
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 3,948,961
Number of Sequences: 7669
Number of extensions: 44492
Number of successful extensions: 1396
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 41
effective length of database: 934,139
effective search space used: 127042904
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)