TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3198766.2.1
(1273 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U41472.1|MSU41472 Medicago sativa pectate lyase homolog ... 215 e-135
gb|CO513899.1|CO513899 s13dSG73H0300028_156460 Glandular tr... 154 3e-038
>gb|U41472.1|MSU41472 Medicago sativa pectate lyase homolog gene, complete cds
Length = 3824
Score = 215 bits (465), Expect(3) = e-135
Identities = 85/128 (66%), Positives = 98/128 (76%)
Frame = +2 / +3
Query: 626 VMLFSSSDKHPEDQIMQITVAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSK 805
VMLF +SD + +D+IMQITVAFNHFG+GL+QRMPRCRWGFFHV+NNDYTHW+MYAIGGS
Sbjct: 2862 VMLFGASDTYQDDKIMQITVAFNHFGQGLIQRMPRCRWGFFHVLNNDYTHWIMYAIGGSS 3041
Query: 806 APTIISQGNRYIAPPNLAAKQVTKQHDTPESVWKNWVWHSENDLFMEGAYFTVTGGQINR 985
APTI+SQGNR+IAP N AAK VT + PESVW W W SE D FM GA F +G I
Sbjct: 3042 APTILSQGNRFIAPHNNAAKTVTHRDYAPESVWSKWQWRSEGDHFMNGATFIQSGPPIKN 3221
Query: 986 QFNKKDLI 1009
KK +
Sbjct: 3222 LPFKKGFL 3245
Score = 182 bits (391), Expect(3) = e-135
Identities = 69/118 (58%), Positives = 86/118 (72%)
Frame = +2 / +2
Query: 14 RVDCRATNPIDRCWRCRQDWATDRKRLARCAKGFGRNTTGGLAGKFYXXXXXXXXXXXNP 193
R C ATNPID CWRCR+DWA +R+ LA+CAKGFGR TTGGL G+ Y NP
Sbjct: 2114 RARCMATNPIDSCWRCRKDWAENRQLLAKCAKGFGRRTTGGLGGRIYVVTDPSDNDMVNP 2293
Query: 194 RPGTLRWGVIQIEPLWITFAKTMIITLKEELIIRGDKTIDGRGVQVRITNGAQLTLQF 367
RPGTLR+G +Q PLWI F ++M+ITL +EL++ DKTIDGRG V+I +GA +T+QF
Sbjct: 2294 RPGTLRFGAVQRGPLWIIFQRSMVITLTQELMVSSDKTIDGRGANVQIRDGAGITMQF 2467
Score = 134 bits (287), Expect(5) = 3e-067
Identities = 69/125 (55%), Positives = 77/125 (61%)
Frame = -2 / -2
Query: 999 FLLNCLLIWPPVTVK*APSMKRSFSECHTQFFHTDSGVSCCLVTCFAARLGGAM*RLPWL 820
FL LI P + AP MK S S+ H F TDSG VT FAA L GAM R P L
Sbjct: 3235 FLNGRFLIGGPD*INVAPFMK*SPSDLHCHFDQTDSGA*SLCVTVFAALL*GAMKRFP*L 3056
Query: 819 MMVGALLPPMAYMSQCV*SLLTTWKKPQRHLGILCTRPLPKWLKATVICMIWSSGCLSLE 640
+VGA LPP+AYM QCV*SL TWK P HLGIL P PKWL ATVIC+I SS +SL
Sbjct: 3055 RIVGAELPPIAYMIQCV*SLFKTWKNPHLHLGIL*INPCPKWLNATVICIILSS*YVSLA 2876
Query: 639 LKSMT 625
++T
Sbjct: 2875 PNNIT 2861
Score = 127 bits (273), Expect(3) = e-135
Identities = 47/71 (66%), Positives = 60/71 (84%)
Frame = +2 / +2
Query: 422 KDGGYVRDSPDHFGWRTVSDGDGITVFGSTNVWLDHLSLSNCQDGLIDVIAKSTGVTISN 601
++GG +RDS DH G RT SDGD I+VFGS+N+W+DH+SLS+C+DGL+DVI ST VTISN
Sbjct: 2516 RNGGLIRDSFDHIGVRTRSDGDAISVFGSSNIWIDHISLSDCEDGLVDVIQGSTAVTISN 2695
Query: 602 CHLTNHNDVML 634
CH+T HNDV +
Sbjct: 2696 CHMTKHNDVSI 2728
Score = 73.9 bits (155), Expect(5) = 3e-067
Identities = 37/75 (49%), Positives = 41/75 (54%)
Frame = -2 / -3
Query: 249 KVIHSGSIWMTPHRSVPGRGFXXXXXXXXXXX*NLPASPPVVLRPNPLAHRARRFRSVAQ 70
K+I SG + P+R VPG GF * LP SPPVVL PNP AH A R AQ
Sbjct: 2349 KIIQSGPL*TAPNRRVPGLGFTISLSEGSVTT*ILPPSPPVVLLPNPFAHLANS*RFSAQ 2170
Query: 69 SCRHRQHRSIGFVAL 25
S H Q SIG VA+
Sbjct: 2169 SFLHLQQLSIGLVAI 2125
Score = 63.4 bits (132), Expect(5) = 3e-067
Identities = 35/68 (51%), Positives = 40/68 (58%)
Frame = -2 / -3
Query: 630 MTSLWLVRWQLEMVTPVDLAMTSMRPSWQLERERWSSHTLVEPKTVMPSPSETVRQPKWS 451
+TSL LV WQ EMVT V+ MTS RPS Q ++E S EPKT + SPS V P S
Sbjct: 2724 LTSLCLVMWQFEMVTAVEP*MTSTRPSSQSDKEI*SIQIFDEPKTEIASPSLLVLTPI*S 2545
Query: 450 GESRT*PP 427
ES PP
Sbjct: 2544 KESLIKPP 2521
Score = 36.3 bits (73), Expect(5) = 3e-067
Identities = 18/34 (52%), Positives = 21/34 (61%)
Frame = -2 / -2
Query: 1089 *QGLPGVQAREPA*RVSLVT*DPFLGLMRSFLLN 988
* GLP Q P RVSL+ *DP LG +R+ LN
Sbjct: 3328 *HGLPTTQLSAPEKRVSLLA*DPCLGFIRNPFLN 3227
Score = 34.5 bits (69), Expect(5) = 3e-067
Identities = 15/24 (62%), Positives = 17/24 (70%)
Frame = -2 / -3
Query: 369 TNCNVSCAPLVMRTCTPRPSMVLS 298
TNC V AP ++ T PRPSMVLS
Sbjct: 2469 TNCMVIPAPSLICTLAPRPSMVLS 2398
>gb|CO513899.1|CO513899 s13dSG73H0300028_156460 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 584
Score = 154 bits (330), Expect = 3e-038
Identities = 62/119 (52%), Positives = 76/119 (63%)
Frame = +2 / +3
Query: 11 GRVDCRATNPIDRCWRCRQDWATDRKRLARCAKGFGRNTTGGLAGKFYXXXXXXXXXXXN 190
G + C + NPID CWRC +W +R+RLA CA GFG+N GG GK Y
Sbjct: 186 GYLSCGSGNPIDDCWRCDPNWEKNRQRLADCAIGFGKNAIGGKNGKIYVVTDSGDDDPVT 365
Query: 191 PRPGTLRWGVIQIEPLWITFAKTMIITLKEELIIRGDKTIDGRGVQVRITNGAQLTLQF 367
P+PGTLR+ VIQ EPLWI FA+ M+I LKEELI+ KTIDGRG V I G +T+Q+
Sbjct: 366 PKPGTLRFAVIQDEPLWIIFARDMVIQLKEELIMNSFKTIDGRGASVHIAGGPCITIQY 542
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,944,001
Number of Sequences: 7669
Number of extensions: 88969
Number of successful extensions: 612
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 349144380
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)