TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3191640.2.1
(923 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY219848.1| Medicago sativa polygalactorunase PG11 precu... 192 4e-062
emb|Y11118.1|MSPOLGAL3 M.sativa PG3 gene 181 2e-055
gb|U20431.1|MSU20431 Medicago sativa polygalacturonase mRNA... 197 2e-051
>gb|AY219848.1| Medicago sativa polygalactorunase PG11 precursor, gene, complete cds
Length = 2213
Score = 192 bits (413), Expect(2) = 4e-062
Identities = 76/165 (46%), Positives = 105/165 (63%)
Frame = +1 / +1
Query: 10 NGTISGIKLLNSKFFHINIDTSKDITVKDVNITAPADVENTDGVHVGGSSNVRITNSTIG 189
N + G+ +SK FH+ + + T +TAP D NTDG+H+G S++++I N+ I
Sbjct: 1234 NSMVHGLTSKDSKNFHVMVLGCNNFTFDGFTVTAPGDSPNTDGIHMGRSTDIKILNTNIA 1413
Query: 190 TGDDCVSIGPGSDGVMVNNITCGPGQGISVGCLGRYKDEKDVSDVTVRDCVLRNTTNGVR 369
TGDDC+S+G GS + V N+ CGPG GISVG LG+Y E +V V V++C L T NGVR
Sbjct: 1414 TGDDCISLGDGSRKITVQNVKCGPGHGISVGSLGKYTTEDNVEGVIVKNCTLTATQNGVR 1593
Query: 370 IKSYEDAESVLTASHLTFENIRMEEVANPIIIDQYFCPQKVCPGK 504
IK++ DA +T S + FE+I M V NP+IIDQ +CP C K
Sbjct: 1594 IKTWPDAPGTITVSDIHFEDIIMNNVMNPVIIDQEYCPWNQCSKK 1728
Score = 100 bits (214), Expect(2) = 1e-024
Identities = 55/148 (37%), Positives = 67/148 (45%)
Frame = -1 / -1
Query: 488 FWGQKYWSMMMGLATSSILMFSKVRWDAVSTDSASSYDLMRTPLVVFLSTQXXXXXXXXX 309
F GQ WSMM G T +++ SK + V AS L+ TP V + Q
Sbjct: 1712 FHGQYSWSMMTGFMTLFMIISSKCISETVIVPGASGQVLILTPF*VAVRVQFLTITPSTL 1533
Query: 308 XXXXXRPRQPTLMPCPGPQVMLFTMTPSLPGPMETQSSPVPMVELVMRTLEDPPTWTPSV 129
PR PTL+P PGP +T+ LP P E QSSPV M+ L + D P W PSV
Sbjct: 1532 SSVVYFPRLPTLIP*PGPHFTFWTVIFLLPSPNEIQSSPVAMLVLRILISVDLPMWIPSV 1353
Query: 128 FSTSAGAVMFTSFTVMSLEVSMLMWKNL 45
S GAV V L + WK L
Sbjct: 1352 LGLSPGAVTVKPSNVKLLHPKTITWKFL 1269
Score = 61.1 bits (127), Expect(2) = 4e-062
Identities = 23/50 (46%), Positives = 32/50 (64%)
Frame = +1 / +3
Query: 511 NSSHVSIKDVTFRNISGTSSTPEAVSLLCSETQPCSGVSLIDVNVEYAGK 660
N S + + ++F+NI GTS TPE V L+CS PC GV L +V + + GK
Sbjct: 1845 NPSKIKLSKISFKNIQGTSGTPEGVVLICSSGVPCDGVELNNVGLTFNGK 1994
Score = 30.9 bits (61), Expect(2) = 1e-024
Identities = 15/29 (51%), Positives = 16/29 (55%)
Frame = -1 / -2
Query: 638 TSMSETPLHGCVSEQSRLTASGVDDVPEM 552
T S TP HG Q R T SGV +VP M
Sbjct: 1972 TLFSSTPSHGTPLLQIRTTPSGVPEVP*M 1886
>emb|Y11118.1|MSPOLGAL3 M.sativa PG3 gene
Length = 2185
Score = 181 bits (390), Expect(2) = 2e-055
Identities = 72/152 (47%), Positives = 99/152 (65%)
Frame = +1 / +1
Query: 40 NSKFFHINIDTSKDITVKDVNITAPADVENTDGVHVGGSSNVRITNSTIGTGDDCVSIGP 219
+SK FH+N+ + T V ITAP NTDG+H+G S++V++ N+ I TGDDCVS+G
Sbjct: 1243 DSKNFHVNVLGCTNFTFDSVTITAPVTSINTDGIHIGRSTDVKVLNTNIATGDDCVSLGD 1422
Query: 220 GSDGVMVNNITCGPGQGISVGCLGRYKDEKDVSDVTVRDCVLRNTTNGVRIKSYEDAESV 399
GS + V N+ CGPG GISVG LG+Y E+ V V V++C + NT NGVRIK++ +
Sbjct: 1423 GSRQITVQNVNCGPGHGISVGSLGKYPKEEAVEHVLVKNCTITNTDNGVRIKTWPSSPGT 1602
Query: 400 LTASHLTFENIRMEEVANPIIIDQYFCPQKVC 495
+ + FE+I M V NP+IIDQ +CP C
Sbjct: 1603 SPITDMHFEDIIMVNVLNPVIIDQEYCPWNQC 1698
Score = 49.2 bits (101), Expect(2) = 2e-055
Identities = 20/47 (42%), Positives = 29/47 (61%)
Frame = +1 / +2
Query: 517 SHVSIKDVTFRNISGTSSTPEAVSLLCSETQPCSGVSLIDVNVEYAG 657
S + I V F+NI GTS T + V L+CS++ PC V L +V + + G
Sbjct: 1808 SKIKISKVIFKNIRGTSKTKDGVVLICSKSFPCDAVELNNVALTFNG 1948
>gb|U20431.1|MSU20431 Medicago sativa polygalacturonase mRNA, complete cds
Length = 1492
Score = 197 bits (424), Expect = 2e-051
Identities = 80/173 (46%), Positives = 111/173 (64%)
Frame = +1 / +1
Query: 10 NGTISGIKLLNSKFFHINIDTSKDITVKDVNITAPADVENTDGVHVGGSSNVRITNSTIG 189
N + G+ +SK FH+ + K+IT ITAP D NTDG+H+G S++V+I N+ IG
Sbjct: 499 NSIVRGVTSKDSKNFHVMVFGCKNITFDGFTITAPGDSPNTDGIHMGKSTDVKILNTNIG 678
Query: 190 TGDDCVSIGPGSDGVMVNNITCGPGQGISVGCLGRYKDEKDVSDVTVRDCVLRNTTNGVR 369
TGDDCVSIG GS + V + CGPG G+SVG LG++ E++V +TV++C L T NGVR
Sbjct: 679 TGDDCVSIGDGSKQITVQGVNCGPGHGLSVGSLGKFTTEENVEGITVKNCTLTATDNGVR 858
Query: 370 IKSYEDAESVLTASHLTFENIRMEEVANPIIIDQYFCPQKVCPGKRSNSSHVS 528
IK++ DA +T S + FE+I M V NP+IIDQ + P C K + +S
Sbjct: 859 IKTWPDAPGTITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCSKKNPSKIKLS 1017
Score = 113 bits (241), Expect(2) = 7e-029
Identities = 61/162 (37%), Positives = 79/162 (48%)
Frame = -1 / -3
Query: 488 FWGQKYWSMMMGLATSSILMFSKVRWDAVSTDSASSYDLMRTPLVVFLSTQXXXXXXXXX 309
F G WSMM G T +++ SK + V AS L+ TPL V + Q
Sbjct: 977 FHG*YSWSMMTGFLTFVMVISSKCISETVIVPGASGQVLILTPLSVAVRVQFLTVIPSTF 798
Query: 308 XXXXXRPRQPTLMPCPGPQVMLFTMTPSLPGPMETQSSPVPMVELVMRTLEDPPTWTPSV 129
PR PTL P PGPQ +T+ LP P+ETQSSPVPM+ L + T D P W PSV
Sbjct: 797 SSVVNFPRLPTLRP*PGPQFTPWTVICLLPSPIETQSSPVPMLVLRILTSVDFPMWIPSV 618
Query: 128 FSTSAGAVMFTSFTVMSLEVSMLMWKNLELSSLIPEIVPLVS 3
S GAV+ V+ L + WK L + P + L++
Sbjct: 617 LGLSPGAVIVKPSNVIFLHPKTITWKFLLSLEVTPRTMELLT 492
Score = 28.6 bits (56), Expect(2) = 7e-029
Identities = 13/27 (48%), Positives = 14/27 (51%)
Frame = -1 / -3
Query: 638 TSMSETPLHGCVSEQSRLTASGVDDVP 558
T S TP HG Q R T S V +VP
Sbjct: 1121 TLFSSTPSHGTALLQIRTTPSAVPEVP 1041
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,499,248
Number of Sequences: 7669
Number of extensions: 60634
Number of successful extensions: 682
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 242563464
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)