TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3184970.2.1
(1043 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U83592.1|MSU83592 Medicago sativa class I chitinase mRNA... 140 8e-087
gb|U83591.1|MSU83591 Medicago sativa class I chitinase mRNA... 140 8e-087
gb|AY804254.1| Medicago sativa chitinase (Chi) mRNA, comple... 138 3e-073
>gb|U83592.1|MSU83592 Medicago sativa class I chitinase mRNA, complete cds
Length = 1267
Score = 140 bits (301), Expect(3) = 8e-087
Identities = 51/88 (57%), Positives = 63/88 (71%)
Frame = -1 / +2
Query: 989 VASIITEDLFERMLKHRNEPDCKARGFYTYDAFITAADAFRGFGTTGSTEVQKRELAAFL 810
+ S+I+ D F MLKHR++ C+ +G YTYDAFI+AA AF F G T +KRE+AAFL
Sbjct: 248 LGSLISRDTFNNMLKHRDDSGCQGKGLYTYDAFISAAKAFPNFANNGDTATKKREIAAFL 427
Query: 809 GQTGHETTGGWPNAPDGAFTWGYCYKEE 726
GQT HETTGGW APDG + WGYC+ E
Sbjct: 428 GQTSHETTGGWATAPDGPYAWGYCFVRE 511
Score = 138 bits (295), Expect(3) = 8e-087
Identities = 53/84 (63%), Positives = 65/84 (77%)
Frame = -1 / +2
Query: 629 LSYNYNYGPAGEDATIAQDLLSNPELVASDAVISFKTAIWFWMTAQPPKPSCHDVATEQW 450
+ ++NY I DLL+NP+LVA+D VISFKTA+WFWMT Q PKPSCHDV T +W
Sbjct: 590 IQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPKPSCHDVITGRW 769
Query: 449 TPSAADKAAGRLPGYGVITNIING 378
+PS+AD+AAGRL GYG +TNIING
Sbjct: 770 SPSSADRAAGRLSGYGTVTNIING 841
Score = 76.2 bits (160), Expect(3) = 8e-087
Identities = 27/48 (56%), Positives = 36/48 (75%)
Frame = -1 / +2
Query: 389 IINGIECGKGYNEKVANRTFFYTSYCDILGISYGDNLDCYNQRPFNSA 246
I G+ECG+G + +V +R FY YCDILG+ YG NLDC++QRPF S+
Sbjct: 833 INGGLECGRGQDGRVQDRIGFYKRYCDILGVGYGANLDCFSQRPFGSS 976
Score = 73.5 bits (154), Expect(4) = 4e-036
Identities = 41/77 (53%), Positives = 44/77 (57%)
Frame = +1 / -2
Query: 739 Q*PQVKAPSGAFGQPPVVSCPVCPKNAASSRFWTSVLPVVPKPRNASAAVMKASYV*KPR 918
Q PQ PSGA PPVVSC V PK AA S F +V P++ K NA AA MKAS V P
Sbjct: 498 QYPQAYGPSGAVAHPPVVSCDV*PKKAAISLFLVAVSPLLAKLGNALAAEMKAS*VYNPF 319
Query: 919 ALQSGSLRCFSILSKRS 969
S RC SIL K S
Sbjct: 318 P*HPLSSRCLSILLKVS 268
Score = 67.0 bits (140), Expect(4) = 4e-036
Identities = 29/45 (64%), Positives = 33/45 (73%)
Frame = +1 / -2
Query: 442 EGVHCSVATSWHDGLGGCAVIQNQMAVLKEMTASDATSSGLLSRS 576
EG+H V TSW DGLG VIQN AVLK++T S AT SGLLS+S
Sbjct: 777 EGLHLPVITSWQDGLGDWGVIQNHRAVLKDITGSVATRSGLLSKS 643
Score = 35.0 bits (70), Expect(4) = 4e-036
Identities = 15/27 (55%), Positives = 16/27 (59%)
Frame = +1 / -2
Query: 598 PAGP*L*L*ESWMGPRPKYFLPATHGH 678
P P*L* E WMGP P Y P HG+
Sbjct: 627 PHCP*L*FQEIWMGPLP*YCFPEAHGN 547
Score = 26.3 bits (51), Expect(4) = 4e-036
Identities = 15/31 (48%), Positives = 19/31 (61%)
Frame = +1 / -2
Query: 256 KGLWL*QSKLSP*LMPRMSQ*LV*KKVRLAT 348
KGL QS+L+P P MSQ L+* +R T
Sbjct: 966 KGL*EKQSRLAPYPTPSMSQYLL*NPIRSCT 874
>gb|U83591.1|MSU83591 Medicago sativa class I chitinase mRNA, complete cds
Length = 1259
Score = 140 bits (301), Expect(3) = 8e-087
Identities = 51/88 (57%), Positives = 63/88 (71%)
Frame = -1 / +1
Query: 989 VASIITEDLFERMLKHRNEPDCKARGFYTYDAFITAADAFRGFGTTGSTEVQKRELAAFL 810
+ S+I+ D F MLKHR++ C+ +G YTYDAFI+AA AF F G T +KRE+AAFL
Sbjct: 247 LGSLISRDTFNNMLKHRDDSGCQGKGLYTYDAFISAAKAFPNFANNGDTATKKREIAAFL 426
Query: 809 GQTGHETTGGWPNAPDGAFTWGYCYKEE 726
GQT HETTGGW APDG + WGYC+ E
Sbjct: 427 GQTSHETTGGWATAPDGPYAWGYCFVRE 510
Score = 138 bits (295), Expect(3) = 8e-087
Identities = 53/84 (63%), Positives = 65/84 (77%)
Frame = -1 / +1
Query: 629 LSYNYNYGPAGEDATIAQDLLSNPELVASDAVISFKTAIWFWMTAQPPKPSCHDVATEQW 450
+ ++NY I DLL+NP+LVA+D VISFKTA+WFWMT Q PKPSCHDV T +W
Sbjct: 589 IQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPKPSCHDVITGRW 768
Query: 449 TPSAADKAAGRLPGYGVITNIING 378
+PS+AD+AAGRL GYG +TNIING
Sbjct: 769 SPSSADRAAGRLSGYGTVTNIING 840
Score = 76.2 bits (160), Expect(3) = 8e-087
Identities = 27/48 (56%), Positives = 36/48 (75%)
Frame = -1 / +1
Query: 389 IINGIECGKGYNEKVANRTFFYTSYCDILGISYGDNLDCYNQRPFNSA 246
I G+ECG+G + +V +R FY YCDILG+ YG NLDC++QRPF S+
Sbjct: 832 INGGLECGRGQDGRVQDRIGFYKRYCDILGVGYGANLDCFSQRPFGSS 975
Score = 73.5 bits (154), Expect(4) = 4e-036
Identities = 41/77 (53%), Positives = 44/77 (57%)
Frame = +1 / -1
Query: 739 Q*PQVKAPSGAFGQPPVVSCPVCPKNAASSRFWTSVLPVVPKPRNASAAVMKASYV*KPR 918
Q PQ PSGA PPVVSC V PK AA S F +V P++ K NA AA MKAS V P
Sbjct: 497 QYPQAYGPSGAVAHPPVVSCDV*PKKAAISLFLVAVSPLLAKLGNALAAEMKAS*VYNPF 318
Query: 919 ALQSGSLRCFSILSKRS 969
S RC SIL K S
Sbjct: 317 P*HPLSSRCLSILLKVS 267
Score = 67.0 bits (140), Expect(4) = 4e-036
Identities = 29/45 (64%), Positives = 33/45 (73%)
Frame = +1 / -1
Query: 442 EGVHCSVATSWHDGLGGCAVIQNQMAVLKEMTASDATSSGLLSRS 576
EG+H V TSW DGLG VIQN AVLK++T S AT SGLLS+S
Sbjct: 776 EGLHLPVITSWQDGLGDWGVIQNHRAVLKDITGSVATRSGLLSKS 642
Score = 35.0 bits (70), Expect(4) = 4e-036
Identities = 15/27 (55%), Positives = 16/27 (59%)
Frame = +1 / -1
Query: 598 PAGP*L*L*ESWMGPRPKYFLPATHGH 678
P P*L* E WMGP P Y P HG+
Sbjct: 626 PHCP*L*FQEIWMGPLP*YCFPEAHGN 546
Score = 26.3 bits (51), Expect(4) = 4e-036
Identities = 15/31 (48%), Positives = 19/31 (61%)
Frame = +1 / -1
Query: 256 KGLWL*QSKLSP*LMPRMSQ*LV*KKVRLAT 348
KGL QS+L+P P MSQ L+* +R T
Sbjct: 965 KGL*EKQSRLAPYPTPSMSQYLL*NPIRSCT 873
>gb|AY804254.1| Medicago sativa chitinase (Chi) mRNA, complete cds
Length = 987
Score = 138 bits (295), Expect(4) = 3e-073
Identities = 53/84 (63%), Positives = 65/84 (77%)
Frame = -1 / +1
Query: 629 LSYNYNYGPAGEDATIAQDLLSNPELVASDAVISFKTAIWFWMTAQPPKPSCHDVATEQW 450
+ ++NY I DLL+NP+LVA+D VISFKTA+WFWMT Q PKPSCHDV T +W
Sbjct: 559 IQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPKPSCHDVITGRW 738
Query: 449 TPSAADKAAGRLPGYGVITNIING 378
+PS+AD+AAGRL GYG +TNIING
Sbjct: 739 SPSSADRAAGRLSGYGTVTNIING 810
Score = 79.9 bits (168), Expect(4) = 3e-073
Identities = 31/61 (50%), Positives = 40/61 (65%)
Frame = -1 / +1
Query: 989 VASIITEDLFERMLKHRNEPDCKARGFYTYDAFITAADAFRGFGTTGSTEVQKRELAAFL 810
+ S+I+ D F MLKHR++ C+ +G YTYDAFI+AA AF F G T +KRE FL
Sbjct: 214 LGSLISRDTFNNMLKHRDDSGCQGKGLYTYDAFISAAKAFPNFANNGDTATKKREDCCFL 393
Query: 809 G 807
G
Sbjct: 394 G 396
Score = 76.2 bits (160), Expect(4) = 3e-073
Identities = 27/48 (56%), Positives = 36/48 (75%)
Frame = -1 / +1
Query: 389 IINGIECGKGYNEKVANRTFFYTSYCDILGISYGDNLDCYNQRPFNSA 246
I G+ECG+G + +V +R FY YCDILG+ YG NLDC++QRPF S+
Sbjct: 802 INGGLECGRGQDGRVQDRIGFYKRYCDILGVGYGANLDCFSQRPFGSS 945
Score = 67.0 bits (140), Expect(4) = 1e-024
Identities = 29/45 (64%), Positives = 33/45 (73%)
Frame = +1 / -2
Query: 442 EGVHCSVATSWHDGLGGCAVIQNQMAVLKEMTASDATSSGLLSRS 576
EG+H V TSW DGLG VIQN AVLK++T S AT SGLLS+S
Sbjct: 746 EGLHLPVITSWQDGLGDWGVIQNHRAVLKDITGSVATRSGLLSKS 612
Score = 40.5 bits (82), Expect(4) = 1e-024
Identities = 25/54 (46%), Positives = 29/54 (53%)
Frame = +1 / -2
Query: 808 PKNAASSRFWTSVLPVVPKPRNASAAVMKASYV*KPRALQSGSLRCFSILSKRS 969
P+ SS F +V P++ K NA AA MKAS V P S RC SIL K S
Sbjct: 395 PRKQQSSLFLVAVSPLLAKLGNALAAEMKAS*VYNPFP*HPLSSRCLSILLKVS 234
Score = 35.0 bits (70), Expect(4) = 1e-024
Identities = 15/27 (55%), Positives = 16/27 (59%)
Frame = +1 / -2
Query: 598 PAGP*L*L*ESWMGPRPKYFLPATHGH 678
P P*L* E WMGP P Y P HG+
Sbjct: 596 PHCP*L*FQEIWMGPLP*YCFPEAHGN 516
Score = 33.6 bits (67), Expect(4) = 3e-073
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = -1 / +1
Query: 782 GWPNAPDGAFTWGYCYKEE 726
G APDG + WGYC+ E
Sbjct: 424 GGATAPDGPYAWGYCFVRE 480
Score = 26.3 bits (51), Expect(4) = 1e-024
Identities = 15/31 (48%), Positives = 19/31 (61%)
Frame = +1 / -2
Query: 256 KGLWL*QSKLSP*LMPRMSQ*LV*KKVRLAT 348
KGL QS+L+P P MSQ L+* +R T
Sbjct: 935 KGL*EKQSRLAPYPTPSMSQYLL*NPIRSCT 843
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,709,732
Number of Sequences: 7669
Number of extensions: 65373
Number of successful extensions: 604
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 279315504
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)