TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3184927.2.1
(1157 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512887.1|CO512887 s13dSG20H0700060_121700 Glandular tr... 130 7e-041
gb|CO512956.1|CO512956 s13dSG86G1100084_121838 Glandular tr... 128 2e-040
gb|U28213.1|MSU28213 Medicago sativa vestitone reductase mR... 58 2e-032
emb|X80222.1|MSDFR1 M.sativa mRNA for dihydroflavonol-4-red... 100 4e-027
gb|CO515075.1|CO515075 s13dSG97E0800067_397655 Glandular tr... 100 3e-022
>gb|CO512887.1|CO512887 s13dSG20H0700060_121700 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 602
Score = 130 bits (279), Expect(2) = 7e-041
Identities = 53/80 (66%), Positives = 63/80 (78%)
Frame = -1 / +1
Query: 1130 VCVTGASGFVASWLIKRLLESGYHVVGTVRDPGNHQKTAHLWKLPGAKERLQIVRADLLE 951
VCVTGASG++ASWL++ LL GY V TVRDP + +K HL KL GAKERLQ+ +A+LLE
Sbjct: 70 VCVTGASGYIASWLVRLLLNRGYTVKATVRDPNDPKKGDHLVKLEGAKERLQLFKANLLE 249
Query: 950 EGSFDSAVMACEGVFHTASP 891
EG+FDS V C GVFHTASP
Sbjct: 250 EGAFDSVVQGCHGVFHTASP 309
Score = 79.0 bits (166), Expect(3) = 1e-026
Identities = 42/80 (52%), Positives = 47/80 (58%)
Frame = +1 / -1
Query: 892 GDAVWNTPSQAITALSKLPSSNRSARTICSLSLAPGNFQRWAVFW*FPGSLTVPTT*YPD 1071
GDAV TP Q T SK PSS+R A C+LSLAP + +W+ F GS TV T P
Sbjct: 308 GDAV*KTP*QP*TTESKAPSSSRFALNSCNLSLAPSSLTKWSPFLGSFGSRTVALTV*PR 129
Query: 1072 SRSRLISQEATKPEAPVTHT 1131
RS S EA PEAPVTHT
Sbjct: 128 LRSNRTSHEAM*PEAPVTHT 69
Score = 51.9 bits (107), Expect(2) = 7e-041
Identities = 22/31 (70%), Positives = 26/31 (83%)
Frame = -1 / +1
Query: 845 PAVNGTLNVLRSCKKNPFLKRVVLTSSSSAV 753
PA+ GTLNVL SC K+P LKR+VLTSS +AV
Sbjct: 352 PALKGTLNVLNSCAKSPSLKRLVLTSSIAAV 444
Score = 37.7 bits (76), Expect(3) = 1e-026
Identities = 17/40 (42%), Positives = 22/40 (55%)
Frame = +1 / -1
Query: 595 SMPFSLANSHAAFSANTLARAYHRCIFSQSGTELHVVSSS 714
SM F+L N HAA SAN YHR + ++ VSS+
Sbjct: 599 SMLFTLTNFHAASSANVFETTYHRFDLAHKSASVNQVSST 480
Score = 35.4 bits (71), Expect(3) = 1e-026
Identities = 16/35 (45%), Positives = 20/35 (57%)
Frame = +1 / -1
Query: 745 LILTADDEDVRTTLFRNGFFLHDLSTFRVPFTAGT 849
L TA EDV+T +G H+L T RVP AG+
Sbjct: 452 LYATAAIEDVKTRRLSDGDLAHELRTLRVPLRAGS 348
>gb|CO512956.1|CO512956 s13dSG86G1100084_121838 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 584
Score = 128 bits (275), Expect(2) = 2e-040
Identities = 53/80 (66%), Positives = 62/80 (77%)
Frame = -1 / +1
Query: 1130 VCVTGASGFVASWLIKRLLESGYHVVGTVRDPGNHQKTAHLWKLPGAKERLQIVRADLLE 951
VCVTGASG++ASWL+ LL GY V TVRDP + +K HL KL GAKERLQ+ +A+LLE
Sbjct: 70 VCVTGASGYIASWLV*LLLNRGYTVKATVRDPNDPKKVDHLVKLEGAKERLQLFKANLLE 249
Query: 950 EGSFDSAVMACEGVFHTASP 891
EG+FDS V C GVFHTASP
Sbjct: 250 EGAFDSVVQGCHGVFHTASP 309
Score = 79.9 bits (168), Expect(3) = 5e-025
Identities = 42/80 (52%), Positives = 47/80 (58%)
Frame = +1 / -1
Query: 892 GDAVWNTPSQAITALSKLPSSNRSARTICSLSLAPGNFQRWAVFW*FPGSLTVPTT*YPD 1071
GDAV TP Q T SK PSS+R A C+LSLAP + +W+ F GS TV T P
Sbjct: 308 GDAV*KTP*QP*TTESKAPSSSRFALNSCNLSLAPSSLTKWSTFLGSFGSRTVALTV*PR 129
Query: 1072 SRSRLISQEATKPEAPVTHT 1131
RS S EA PEAPVTHT
Sbjct: 128 LRSNQTSHEAM*PEAPVTHT 69
Score = 51.9 bits (107), Expect(2) = 2e-040
Identities = 22/31 (70%), Positives = 26/31 (83%)
Frame = -1 / +1
Query: 845 PAVNGTLNVLRSCKKNPFLKRVVLTSSSSAV 753
PA+ GTLNVL SC K+P LKR+VLTSS +AV
Sbjct: 352 PALKGTLNVLNSCAKSPSLKRLVLTSSIAAV 444
Score = 35.4 bits (71), Expect(3) = 5e-025
Identities = 16/35 (45%), Positives = 20/35 (57%)
Frame = +1 / -1
Query: 745 LILTADDEDVRTTLFRNGFFLHDLSTFRVPFTAGT 849
L TA EDV+T +G H+L T RVP AG+
Sbjct: 452 LYATAAIEDVKTRRLSDGDLAHELRTLRVPLRAGS 348
Score = 31.8 bits (63), Expect(3) = 5e-025
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = +1 / -1
Query: 610 LANSHAAFSANTLARAYHRCIFSQSGTELHVVSSS 714
L N HAA SAN YHR + ++ VSS+
Sbjct: 584 LTNFHAASSANVFETTYHRFDLAHKSASVNQVSST 480
>gb|U28213.1|MSU28213 Medicago sativa vestitone reductase mRNA, complete cds
Length = 1237
Score = 57.9 bits (120), Expect(4) = 2e-032
Identities = 23/51 (45%), Positives = 30/51 (58%)
Frame = -1 / +2
Query: 1040 DPGNHQKTAHLWKLPGAKERLQIVRADLLEEGSFDSAVMACEGVFHTASPV 888
DP + + L LPGA E+L ADL SF +A+ C G+FHTASP+
Sbjct: 167 DPERKRDVSFLTNLPGASEKLHFFNADLSNPDSFAAAIEGCVGIFHTASPI 319
Score = 57.0 bits (118), Expect(4) = 2e-032
Identities = 21/33 (63%), Positives = 27/33 (81%)
Frame = -1 / +2
Query: 1139 KGKVCVTGASGFVASWLIKRLLESGYHVVGTVR 1041
KG+VCVTG +GF+ SW+IK LLE+GY V T+R
Sbjct: 65 KGRVCVTGGTGFLGSWIIKSLLENGYSVNTTIR 163
Score = 49.2 bits (101), Expect(4) = 2e-032
Identities = 20/52 (38%), Positives = 30/52 (57%)
Frame = -1 / +2
Query: 473 RMGYVHIDDVASSHILVYEAPQAAGRYLCSSVVLDNDELVSSLAKRYPIFPI 318
R VH+DDVA +HI + E GRY CS ++ +E+ L+ +YP + I
Sbjct: 725 RFHMVHVDDVARAHIYLLENSVPGGRYNCSPFIVPIEEMSQLLSAKYPEYQI 880
Score = 33.6 bits (67), Expect(4) = 2e-032
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = -1 / +2
Query: 839 VNGTLNVLRSCKKNPFLKRVVLTSSSSAV 753
V+G L +L++C + +KR + TSS SAV
Sbjct: 365 VDGALGILKACVNSKTVKRFIYTSSGSAV 451
>emb|X80222.1|MSDFR1 M.sativa mRNA for dihydroflavonol-4-reductase
Length = 650
Score = 100 bits (212), Expect(2) = 4e-027
Identities = 41/70 (58%), Positives = 51/70 (72%)
Frame = -1 / +1
Query: 1097 SWLIKRLLESGYHVVGTVRDPGNHQKTAHLWKLPGAKERLQIVRADLLEEGSFDSAVMAC 918
SWL+ RL+E GY V TVRDP N +K + L +LPGAK +L I +ADL EEGSFD A+ C
Sbjct: 4 SWLVMRLMEPGYMVRATVRDPENLKKVSPLLELPGAKSKLSIWKADLGEEGSFDEAIKGC 183
Query: 917 EGVFHTASPV 888
GVFH A+P+
Sbjct: 184 TGVFHVATPM 213
Score = 39.1 bits (79), Expect(2) = 4e-027
Identities = 16/51 (31%), Positives = 28/51 (54%)
Frame = -1 / +1
Query: 599 IDLVTVLPSFVIGPSLSHELCVTASDVLGLFQGDTARFSSYGRMGYVHIDD 447
+D++T++P V+GP L + + L G+ A +S + YVH+DD
Sbjct: 496 MDVITIIPPLVVGPFLIPTMPPSLITALSPITGNEAHYSIIKQGQYVHLDD 648
>gb|CO515075.1|CO515075 s13dSG97E0800067_397655 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 346
Score = 100 bits (214), Expect = 3e-022
Identities = 41/65 (63%), Positives = 49/65 (75%)
Frame = -1 / +1
Query: 1130 VCVTGASGFVASWLIKRLLESGYHVVGTVRDPGNHQKTAHLWKLPGAKERLQIVRADLLE 951
VCVTGASGF+ SWL+ RL+E GY V TVRDP N +K HL +LPGA +L + +ADL E
Sbjct: 151 VCVTGASGFIGSWLVMRLIEHGYTVRATVRDPDNIKKVKHLLELPGANSKLSLWKADLGE 330
Query: 950 EGSFD 936
EGSFD
Sbjct: 331 EGSFD 345
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,284,549
Number of Sequences: 7669
Number of extensions: 120109
Number of successful extensions: 1218
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 314229942
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)