TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3183567.2.1
(681 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514897.1|CO514897 s13dSG56F1200103_328454 Glandular tr... 220 4e-060
gb|CO515476.1|CO515476 s13dSG52A0400033_418011 Glandular tr... 219 7e-060
gb|CO516293.1|CO516293 s13dSG69F0900077_445914 Glandular tr... 219 9e-060
gb|CO512077.1|CO512077 s13dSG18F0100011_108474 Glandular tr... 213 2e-059
>gb|CO514897.1|CO514897 s13dSG56F1200103_328454 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 510
Score = 220 bits (475), Expect(2) = 4e-060
Identities = 83/108 (76%), Positives = 96/108 (88%)
Frame = -2 / +1
Query: 674 AGGGIKADLLXXPEYLEQNATLAFMAAMWRWMTPIKKSQPSAHDAFVGNWKPTKNDTLSK 495
AG +K +LL PEY+EQNATLAF AA+W+WMTPIKKSQPSAHDAFVGNWKPTKNDT+ K
Sbjct: 121 AGEALKVNLLDHPEYIEQNATLAFQAAIWKWMTPIKKSQPSAHDAFVGNWKPTKNDTMEK 300
Query: 494 RLPGFGATMNILYGESICGKGYVDAMNVIISHYQYYLDLMGVGREHSG 351
R+PGFGATMNILYGE +CG+G VD+MN I+SHY YYLDL+GVGRE +G
Sbjct: 301 RVPGFGATMNILYGEGVCGQGDVDSMNTIVSHYLYYLDLLGVGREQAG 444
Score = 147 bits (316), Expect = 1e-036
Identities = 70/108 (64%), Positives = 74/108 (68%)
Frame = +1 / -2
Query: 352 PECSRPTPMRSR*YW*CEIITFMASTYPLPQIDSP*SMFMVAPNPGRRLLSVSFLVGFQL 531
P CSRPTP RSR*Y * E + F+ ST P PQ SP* MF+VAP PG R VSFLVGFQL
Sbjct: 443 PACSRPTPRRSR*YR*WETMVFIESTSPCPQTPSP*RMFIVAPKPGTRFSIVSFLVGFQL 264
Query: 532 PTKASCADGWLFLIGVIHRHIAAMNASVAFCSRYSGXXSRSALIPPPA 675
PTKAS A+G FLIGVIH IAA A VAFCS YSG SR PA
Sbjct: 263 PTKAS*AEGCDFLIGVIHFQIAAWKARVAFCSMYSGWSSRFTFRASPA 120
Score = 25.8 bits (50), Expect(2) = 4e-060
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = -2 / +1
Query: 338 CAEQAPFNPSS 306
CAEQ PFNP++
Sbjct: 463 CAEQRPFNPNT 495
>gb|CO515476.1|CO515476 s13dSG52A0400033_418011 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 494
Score = 219 bits (473), Expect(2) = 7e-060
Identities = 83/108 (76%), Positives = 96/108 (88%)
Frame = -2 / +3
Query: 674 AGGGIKADLLXXPEYLEQNATLAFMAAMWRWMTPIKKSQPSAHDAFVGNWKPTKNDTLSK 495
AG +K +LL PEY+EQNATLAF AA+W+WMTPIKKSQPSAHDAFVGNWKPTKNDT+ K
Sbjct: 12 AGEALKVNLLDHPEYIEQNATLAFQAAIWKWMTPIKKSQPSAHDAFVGNWKPTKNDTMEK 191
Query: 494 RLPGFGATMNILYGESICGKGYVDAMNVIISHYQYYLDLMGVGREHSG 351
R+PGFGATMNILYGE +CG+G VD+MN I+SHY YYLDL+GVGRE +G
Sbjct: 192 RVPGFGATMNILYGEGVCGQGDVDSMNSIVSHYLYYLDLLGVGREQAG 335
Score = 148 bits (318), Expect = 6e-037
Identities = 71/108 (65%), Positives = 74/108 (68%)
Frame = +1 / -2
Query: 352 PECSRPTPMRSR*YW*CEIITFMASTYPLPQIDSP*SMFMVAPNPGRRLLSVSFLVGFQL 531
P CSRPTP RSR*Y * E I F+ ST P PQ SP* MF+VAP PG R VSFLVGFQL
Sbjct: 334 PACSRPTPRRSR*YK*WETILFIESTSPCPQTPSP*RMFIVAPKPGTRFSIVSFLVGFQL 155
Query: 532 PTKASCADGWLFLIGVIHRHIAAMNASVAFCSRYSGXXSRSALIPPPA 675
PTKAS A+G FLIGVIH IAA A VAFCS YSG SR PA
Sbjct: 154 PTKAS*AEGCDFLIGVIHFQIAAWKARVAFCSMYSGWSSRFTFRASPA 11
Score = 25.8 bits (50), Expect(2) = 7e-060
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = -2 / +3
Query: 338 CAEQAPFNPSS 306
CAEQ PFNP++
Sbjct: 354 CAEQRPFNPNT 386
>gb|CO516293.1|CO516293 s13dSG69F0900077_445914 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 520
Score = 219 bits (472), Expect(2) = 9e-060
Identities = 83/108 (76%), Positives = 96/108 (88%)
Frame = -2 / +3
Query: 674 AGGGIKADLLXXPEYLEQNATLAFMAAMWRWMTPIKKSQPSAHDAFVGNWKPTKNDTLSK 495
AG +K +LL PEY+EQNATLAF AA+W+WMTPIKKSQPSAHDAFVGNWKPTKNDT+ K
Sbjct: 24 AGEALKVNLLDHPEYIEQNATLAFQAAIWKWMTPIKKSQPSAHDAFVGNWKPTKNDTMEK 203
Query: 494 RLPGFGATMNILYGESICGKGYVDAMNVIISHYQYYLDLMGVGREHSG 351
R+PGFGATMNILYGE +CG+G VD+MN I+SHY YYLDL+GVGRE +G
Sbjct: 204 RVPGFGATMNILYGEGVCGQGDVDSMNNIVSHYLYYLDLLGVGREQAG 347
Score = 148 bits (318), Expect = 6e-037
Identities = 71/108 (65%), Positives = 74/108 (68%)
Frame = +1 / -1
Query: 352 PECSRPTPMRSR*YW*CEIITFMASTYPLPQIDSP*SMFMVAPNPGRRLLSVSFLVGFQL 531
P CSRPTP RSR*Y * E I F+ ST P PQ SP* MF+VAP PG R VSFLVGFQL
Sbjct: 346 PACSRPTPRRSR*YK*WETILFIESTSPCPQTPSP*RMFIVAPKPGTRFSIVSFLVGFQL 167
Query: 532 PTKASCADGWLFLIGVIHRHIAAMNASVAFCSRYSGXXSRSALIPPPA 675
PTKAS A+G FLIGVIH IAA A VAFCS YSG SR PA
Sbjct: 166 PTKAS*AEGCDFLIGVIHFQIAAWKARVAFCSMYSGWSSRFTFRASPA 23
Score = 25.8 bits (50), Expect(2) = 9e-060
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = -2 / +3
Query: 338 CAEQAPFNPSS 306
CAEQ PFNP++
Sbjct: 366 CAEQRPFNPNT 398
>gb|CO512077.1|CO512077 s13dSG18F0100011_108474 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 582
Score = 213 bits (459), Expect(2) = 2e-059
Identities = 80/100 (80%), Positives = 91/100 (91%)
Frame = -2 / +1
Query: 650 LLXXPEYLEQNATLAFMAAMWRWMTPIKKSQPSAHDAFVGNWKPTKNDTLSKRLPGFGAT 471
LL PEY+EQNATLAF AA+W+WMTPIKKSQPSAHDAFVGNWKPTKNDT+ KR+PGFGAT
Sbjct: 4 LLDHPEYIEQNATLAFQAAIWKWMTPIKKSQPSAHDAFVGNWKPTKNDTMEKRVPGFGAT 183
Query: 470 MNILYGESICGKGYVDAMNVIISHYQYYLDLMGVGREHSG 351
MNILYGE +CG+G VD+MN I+SHY YYLDL+GVGRE +G
Sbjct: 184 MNILYGEGVCGQGDVDSMNTIVSHYLYYLDLLGVGREQAG 303
Score = 143 bits (307), Expect = 2e-035
Identities = 67/99 (67%), Positives = 71/99 (71%)
Frame = +1 / -2
Query: 352 PECSRPTPMRSR*YW*CEIITFMASTYPLPQIDSP*SMFMVAPNPGRRLLSVSFLVGFQL 531
P CSRPTP RSR*Y * E + F+ ST P PQ SP* MF+VAP PG R VSFLVGFQL
Sbjct: 302 PACSRPTPRRSR*YR*WETMVFIESTSPCPQTPSP*RMFIVAPKPGTRFSIVSFLVGFQL 123
Query: 532 PTKASCADGWLFLIGVIHRHIAAMNASVAFCSRYSGXXS 648
PTKAS A+G FLIGVIH IAA A VAFCS YSG S
Sbjct: 122 PTKAS*AEGCDFLIGVIHFQIAAWKARVAFCSMYSGWSS 6
Score = 30.4 bits (60), Expect(2) = 2e-059
Identities = 11/33 (33%), Positives = 16/33 (48%)
Frame = -2 / +1
Query: 338 CAEQAPFNPSSPTXXXXXXXXXXXXGLIAPLPL 240
CAEQ PFNP++ ++ P+PL
Sbjct: 322 CAEQRPFNPNTKLAAS*IRSCLPAYDIVVPIPL 420
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,398,511
Number of Sequences: 7669
Number of extensions: 66773
Number of successful extensions: 1041
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 170470480
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)