TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3173057.2.1
(1264 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO515609.1|CO515609 s13dSG47E0500039_418277 Glandular tr... 239 2e-081
gb|CO511782.1|CO511782 s13dSG02A0400033_103464 Glandular tr... 159 3e-070
gb|CO514893.1|CO514893 s13dSG56F0300031_328446 Glandular tr... 138 2e-049
gb|CO515300.1|CO515300 s13dSG48C1200098_417659 Glandular tr... 109 1e-043
gb|CO512362.1|CO512362 s13dSG80A0800053_114238 Glandular tr... 100 1e-031
emb|AJ245868.1|MSA245868 Medicago sativa mRNA for cysteine ... 60 2e-029
>gb|CO515609.1|CO515609 s13dSG47E0500039_418277 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 591
Score = 239 bits (517), Expect(3) = 2e-081
Identities = 90/124 (72%), Positives = 108/124 (87%)
Frame = -2 / +1
Query: 984 NGGIDTEDDYPYRAVDGKCDMNRKNARVVSIDGFEDVPENDEKSLQKAVAHQPVSVAIEA 805
NGGID+E+DYPY+A+DG+CD NRKNA+VV+ID +EDVP DE +LQKAVA+QP++VA+E
Sbjct: 7 NGGIDSEEDYPYKAIDGRCDQNRKNAKVVTIDDYEDVPAYDELALQKAVANQPIAVAVEG 186
Query: 804 GGREFQLYKSGVFSGSCTTNLDHGVVAVGYGAENGKDYWIVRNSWGPKWGEAGYIRMERN 625
GGREFQLY+ GVF+G C T LDHGV AVGYG ENGKDYWIVRNSWG WGE GYIR+ERN
Sbjct: 187 GGREFQLYEYGVFTGRCGTALDHGVAAVGYGTENGKDYWIVRNSWGGSWGEQGYIRLERN 366
Query: 624 VNAS 613
+ +S
Sbjct: 367 LASS 378
Score = 77.1 bits (162), Expect(4) = 6e-026
Identities = 36/73 (49%), Positives = 40/73 (54%)
Frame = +2 / -2
Query: 737 WSRFVVQLPLKTPDL*SWNSRPPASMATLTGW*ATAXXXXXXXXXXXXXXXXMLTTLAFF 916
WS V LP+ TP +WNS PP S AT GW ATA ++TTLAFF
Sbjct: 254 WSSAVPHLPVNTPYSYNWNSLPPPSTATAIGWFATAFCKANSS*AGTSS*SSIVTTLAFF 75
Query: 917 LFMSHFPSTAL*G 955
LF SH PS AL*G
Sbjct: 74 LFWSHLPSIAL*G 36
Score = 64.3 bits (134), Expect(3) = 2e-081
Identities = 19/36 (52%), Positives = 25/36 (69%)
Frame = -2 / +1
Query: 507 NVCDENFSCSAGSTCCCAFGFRNVCLVWGCCPVEGA 400
+VCD +SC+ GSTCCC + + C WGCCP+E A
Sbjct: 484 SVCDSYYSCAEGSTCCCIYEYGRSCFEWGCCPLESA 591
Score = 38.2 bits (77), Expect(4) = 6e-026
Identities = 14/20 (70%), Positives = 15/20 (75%)
Frame = +2 / -2
Query: 557 FAPFFVG*DAIIAIPHFPVL 616
F PF +G*D IAIPHFP L
Sbjct: 437 FCPFLIG*DGSIAIPHFPAL 378
Score = 33.1 bits (66), Expect(3) = 2e-081
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = -2 / +1
Query: 621 NASTGKCGIAMMASYPTKKGAN 556
++ GKCGIA+ SYP K G N
Sbjct: 373 SSRAGKCGIAIEPSYPIKNGQN 438
Score = 27.6 bits (54), Expect(4) = 6e-026
Identities = 11/17 (64%), Positives = 11/17 (64%)
Frame = +2 / -2
Query: 641 M*PASPHFGPHELRTIQ 691
M*P SP PHE TIQ
Sbjct: 350 M*PCSPQLPPHEFLTIQ 300
Score = 24.4 bits (47), Expect(4) = 6e-026
Identities = 15/35 (42%), Positives = 17/35 (48%)
Frame = +2 / -2
Query: 401 APSTGQQPQTKQTFLKPNAQQQVLPAEHEKFSSQT 505
A S GQ P +KQ QQQV P+ SQT
Sbjct: 590 ADSRGQHPHSKQDLPYS*IQQQVEPSAQL**LSQT 486
>gb|CO511782.1|CO511782 s13dSG02A0400033_103464 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 536
Score = 159 bits (342), Expect(3) = 3e-070
Identities = 61/83 (73%), Positives = 71/83 (85%)
Frame = -2 / +2
Query: 861 EKSLQKAVAHQPVSVAIEAGGREFQLYKSGVFSGSCTTNLDHGVVAVGYGAENGKDYWIV 682
E +LQKAVA+QP++VA+E GGREFQLY+ GVF+G C T LDHGV AVGYG ENGKDYWIV
Sbjct: 5 ELALQKAVANQPIAVAVEGGGREFQLYEYGVFTGRCGTALDHGVAAVGYGTENGKDYWIV 184
Query: 681 RNSWGPKWGEAGYIRMERNVNAS 613
RNSWG WGE GYIR+ERN+ +S
Sbjct: 185 RNSWGGSWGEQGYIRLERNLASS 253
Score = 107 bits (228), Expect(3) = 3e-070
Identities = 34/58 (58%), Positives = 41/58 (70%)
Frame = -2 / +2
Query: 507 NVCDENFSCSAGSTCCCAFGFRNVCLVWGCCPVEGATCCKDHASCCPPGYPVCNVRAG 334
+VCD +SC+ GSTCCC + + C WGCCP+E ATCC D SCCP YPVC+ RAG
Sbjct: 359 SVCDSYYSCAEGSTCCCIYEYGGSCFEWGCCPLESATCCDDRYSCCPHEYPVCDTRAG 532
Score = 47.8 bits (98), Expect(4) = 2e-021
Identities = 20/36 (55%), Positives = 22/36 (61%)
Frame = +2 / -3
Query: 737 WSRFVVQLPLKTPDL*SWNSRPPASMATLTGW*ATA 844
WS V LP+ TP +WNS PP S AT GW ATA
Sbjct: 129 WSSAVPHLPVNTPYSYNWNSLPPPSTATAIGWFATA 22
Score = 38.2 bits (77), Expect(4) = 2e-021
Identities = 14/20 (70%), Positives = 15/20 (75%)
Frame = +2 / -3
Query: 557 FAPFFVG*DAIIAIPHFPVL 616
F PF +G*D IAIPHFP L
Sbjct: 312 FCPFLIG*DGSIAIPHFPAL 253
Score = 38.2 bits (77), Expect(4) = 2e-021
Identities = 20/34 (58%), Positives = 21/34 (61%)
Frame = +2 / -3
Query: 335 PALTLQTG*PGGQQLA*SLQQVAPSTGQQPQTKQ 436
PA QTG GQQL S QQVA S GQ P +KQ
Sbjct: 531 PARVSQTGYS*GQQL*RSSQQVADSRGQHPHSKQ 430
Score = 33.1 bits (66), Expect(3) = 3e-070
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = -2 / +2
Query: 621 NASTGKCGIAMMASYPTKKGAN 556
++ GKCGIA+ SYP K G N
Sbjct: 248 SSRAGKCGIAIEPSYPIKNGQN 313
Score = 27.6 bits (54), Expect(4) = 2e-021
Identities = 11/17 (64%), Positives = 11/17 (64%)
Frame = +2 / -3
Query: 641 M*PASPHFGPHELRTIQ 691
M*P SP PHE TIQ
Sbjct: 225 M*PCSPQLPPHEFLTIQ 175
>gb|CO514893.1|CO514893 s13dSG56F0300031_328446 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 620
Score = 138 bits (295), Expect(3) = 2e-049
Identities = 49/88 (55%), Positives = 60/88 (68%)
Frame = -2 / +1
Query: 507 NVCDENFSCSAGSTCCCAFGFRNVCLVWGCCPVEGATCCKDHASCCPPGYPVCNVRAGTC 328
+VCD +SC+ GSTCCC + + C WGCCP+E ATCC DH SCCP YPVC+ RAG C
Sbjct: 190 SVCDSYYSCAEGSTCCCIYEYGRSCFEWGCCPLESATCCDDHYSCCPHEYPVCDTRAGLC 369
Query: 327 SVSKNSPLSVKALKRTLAKLSTA*GPRS 244
KN+PL VK+ KRT AK A G ++
Sbjct: 370 LKGKNNPLGVKSFKRTPAKPHWAFGGKN 453
Score = 58.3 bits (121), Expect(3) = 2e-049
Identities = 20/27 (74%), Positives = 23/27 (85%)
Frame = -2 / +1
Query: 693 YWIVRNSWGPKWGEAGYIRMERNVNAS 613
YWIVRNSWG WGE GYIR+ERN+ +S
Sbjct: 4 YWIVRNSWGGSWGEQGYIRLERNLASS 84
Score = 33.1 bits (66), Expect(3) = 2e-049
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = -2 / +1
Query: 621 NASTGKCGIAMMASYPTKKGAN 556
++ GKCGIA+ SYP K G N
Sbjct: 79 SSRAGKCGIAIEPSYPIKNGQN 144
>gb|CO515300.1|CO515300 s13dSG48C1200098_417659 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 588
Score = 109 bits (233), Expect(2) = 1e-043
Identities = 39/60 (65%), Positives = 54/60 (90%)
Frame = -2 / +2
Query: 1227 EELPESVDWREKGAVAPVKNQGQCGSCWAFSAVSSVESVNQIVTGEMVTLSEQELVECST 1048
++LPESVDWR++GAV VK+Q CGSCWAFSA+++VE +N+IVTG++++LSEQELV+C T
Sbjct: 290 DKLPESVDWRKEGAVVGVKDQASCGSCWAFSAIAAVEGINKIVTGDLISLSEQELVDCDT 469
Score = 82.2 bits (173), Expect(2) = 1e-043
Identities = 28/37 (75%), Positives = 34/37 (91%)
Frame = -2 / +2
Query: 1038 NSGCNGGLMDAAFDFIIKNGGIDTEDDYPYRAVDGKC 928
N GCNGGLMD AF+FII NGGID+E+DYPY+A+DG+C
Sbjct: 476 NEGCNGGLMDYAFEFIISNGGIDSEEDYPYKAIDGRC 586
>gb|CO512362.1|CO512362 s13dSG80A0800053_114238 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 576
Score = 100 bits (214), Expect(2) = 1e-031
Identities = 36/56 (64%), Positives = 45/56 (80%)
Frame = -2 / +1
Query: 1221 LPESVDWREKGAVAPVKNQGQCGSCWAFSAVSSVESVNQIVTGEMVTLSEQELVEC 1054
LPE DWREKGAV PVK+QG CGSCWAFS ++E N + TG++V+LSEQ+LV+C
Sbjct: 277 LPEDFDWREKGAVTPVKDQGSCGSCWAFSTTGALEGANYLATGKLVSLSEQQLVDC 444
Score = 51.0 bits (105), Expect(2) = 1e-031
Identities = 17/30 (56%), Positives = 26/30 (86%)
Frame = -2 / +1
Query: 1038 NSGCNGGLMDAAFDFIIKNGGIDTEDDYPY 949
+SGCNGGLM+ AF++I+++GG+ +E DY Y
Sbjct: 481 DSGCNGGLMNNAFEYILQSGGVVSEKDYAY 570
>emb|AJ245868.1|MSA245868 Medicago sativa mRNA for cysteine protease, partial (cyp15a gene)
Length = 890
Score = 59.7 bits (124), Expect(4) = 2e-029
Identities = 20/41 (48%), Positives = 31/41 (75%)
Frame = -2 / +1
Query: 1038 NSGCNGGLMDAAFDFIIKNGGIDTEDDYPYRAVDGKCDMNR 916
+SGCNGGLM+ AF++I+++GG+ +E DY Y DG C ++
Sbjct: 142 DSGCNGGLMNNAFEYILQSGGVVSEKDYAYTGRDGSCKFDK 264
Score = 54.7 bits (113), Expect(4) = 2e-029
Identities = 19/35 (54%), Positives = 27/35 (77%)
Frame = -2 / +1
Query: 1158 CGSCWAFSAVSSVESVNQIVTGEMVTLSEQELVEC 1054
CGS WAFS ++E N + TG++V+LSEQ+LV+C
Sbjct: 1 CGSGWAFSTTGALEGANYLATGKLVSLSEQQLVDC 105
Score = 47.3 bits (97), Expect(4) = 2e-029
Identities = 15/27 (55%), Positives = 19/27 (70%)
Frame = -2 / +1
Query: 699 KDYWIVRNSWGPKWGEAGYIRMERNVN 619
K YWI++NSWG WGE GY ++ R N
Sbjct: 499 KPYWIIKNSWGQNWGEEGYYKICRGRN 579
Score = 24.9 bits (48), Expect(4) = 2e-029
Identities = 8/12 (66%), Positives = 11/12 (91%)
Frame = -2 / +1
Query: 744 LDHGVVAVGYGA 709
LDHGV+ VG+G+
Sbjct: 433 LDHGVLLVGFGS 468
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,795,254
Number of Sequences: 7669
Number of extensions: 114731
Number of successful extensions: 1011
Number of sequences better than 1.0e-020: 7
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 346387977
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)