TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115190.2.2
(1172 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U41472.1|MSU41472 Medicago sativa pectate lyase homolog ... 212 e-116
>gb|U41472.1|MSU41472 Medicago sativa pectate lyase homolog gene, complete cds
Length = 3824
Score = 212 bits (457), Expect(4) = e-116
Identities = 83/128 (64%), Positives = 104/128 (81%)
Frame = +1 / +2
Query: 1 IFARSMIIQLSQELLMSSDKTIDGRGAQVHIANGAGITVQLAQNVIIHNLHVHDVKHTMG 180
IF RSM+I L+QEL++SSDKTIDGRGA V I +GAGIT+Q NVIIH L + ++K G
Sbjct: 2345 IFQRSMVITLTQELMVSSDKTIDGRGANVQIRDGAGITMQFVNNVIIHGLRIKNIKARNG 2524
Query: 181 GLMRDSPTHIGSRTRADGDGISLFSATNVWIDHISMSNCEDGLIDVVQSSTGITISNCHF 360
GL+RDS HIG RTR+DGD IS+F ++N+WIDHIS+S+CEDGL+DV+Q ST +TISNCH
Sbjct: 2525 GLIRDSFDHIGVRTRSDGDAISVFGSSNIWIDHISLSDCEDGLVDVIQGSTAVTISNCHM 2704
Query: 361 TNHNDVML 384
T HNDV +
Sbjct: 2705 TKHNDVSI 2728
Score = 176 bits (379), Expect(4) = e-116
Identities = 66/83 (79%), Positives = 76/83 (91%)
Frame = +1 / +3
Query: 376 VMLFGASDSWPQDQIMQITVAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGGD 555
VMLFGASD++ D+IMQITVAFNHFG+GL+QRMPRCRWGFFHV+NNDYTHW+MYAIGG
Sbjct: 2862 VMLFGASDTYQDDKIMQITVAFNHFGQGLIQRMPRCRWGFFHVLNNDYTHWIMYAIGGSS 3041
Query: 556 APTIISQGNRYIAPPNIAAKVIT 624
APTI+SQGNR+IAP N AAK +T
Sbjct: 3042 APTILSQGNRFIAPHNNAAKTVT 3110
Score = 105 bits (223), Expect(3) = 6e-052
Identities = 53/104 (50%), Positives = 62/104 (59%)
Frame = -1 / -3
Query: 380 MTSLWLVKWQLEMVIPVELCTTSMRPSSQLDMEMWSIQTLVAEKREMPSPSALVREPMCV 201
+TSL LV WQ EMV VE TS RPSSQ D E+ SIQ K E+ SPS LV P+
Sbjct: 2724 LTSLCLVMWQFEMVTAVEP*MTSTRPSSQSDKEI*SIQIFDEPKTEIASPSLLVLTPI*S 2545
Query: 200 GESRMRPPMVCLTSCTCRLWMMTFCASCTVIPAPLAMCTCAPRP 69
ES ++PP + L R MMT +C VIPAP +CT APRP
Sbjct: 2544 KESLIKPPFLALMFLMRRP*MMTLFTNCMVIPAPSLICTLAPRP 2413
Score = 105 bits (223), Expect(3) = 6e-052
Identities = 48/83 (57%), Positives = 54/83 (65%)
Frame = -1 / -2
Query: 623 VITLAAMFGGAM*RLPWLMMVGASPPPMAYMSQCV*SLLTTWKKPQRHLGIRCTRPLPKW 444
V AA+ GAM R P L +VGA PP+AYM QCV*SL TWK P HLGI P PKW
Sbjct: 3109 VTVFAALL*GAMKRFP*LRIVGAELPPIAYMIQCV*SLFKTWKNPHLHLGIL*INPCPKW 2930
Query: 443 LKATVICMIWSCGQESLAPKSMT 375
L ATVIC+I S SLAP ++T
Sbjct: 2929 LNATVICIILSS*YVSLAPNNIT 2861
Score = 52.4 bits (108), Expect(4) = e-116
Identities = 23/55 (41%), Positives = 27/55 (49%)
Frame = +1 / +3
Query: 601 NIAAKVITKHYAEEGVWKNWVWHTEDDLFMNGAIFNPSGGAPKQVDTNEWVKPKP 765
N A V + YA E VW W W +E D FMNGA F SG K + + KP
Sbjct: 3090 NAAKTVTHRDYAPESVWSKWQWRSEGDHFMNGATFIQSGPPIKNLPFKKGFLMKP 3254
Score = 46.0 bits (94), Expect(4) = e-116
Identities = 16/29 (55%), Positives = 22/29 (75%)
Frame = +1 / +3
Query: 751 VKPKPGTYVTRLTRFSGTLSCCTGKPC*R 837
+KP+ G+ RLTRFSG L+C G+PC*+
Sbjct: 3246 MKPRHGSQANRLTRFSGALNCVVGRPC*K 3332
Score = 37.7 bits (76), Expect(3) = 6e-052
Identities = 17/25 (68%), Positives = 18/25 (72%)
Frame = -1 / -2
Query: 830 HGLPVQQDSVPEKRVSLVT*VPGLG 756
HGLP Q S PEKRVSL+ * P LG
Sbjct: 3325 HGLPTTQLSAPEKRVSLLA*DPCLG 3251
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,171,797
Number of Sequences: 7669
Number of extensions: 137315
Number of successful extensions: 1207
Number of sequences better than 1.0e-020: 1
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 318823947
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)