TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115184.2.3
(1548 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U20431.1|MSU20431 Medicago sativa polygalacturonase mRNA... 293 4e-093
emb|Y11118.1|MSPOLGAL3 M.sativa PG3 gene 202 2e-078
gb|AY219848.1| Medicago sativa polygalactorunase PG11 precu... 221 3e-074
>gb|U20431.1|MSU20431 Medicago sativa polygalacturonase mRNA, complete cds
Length = 1492
Score = 293 bits (635), Expect(2) = 4e-093
Identities = 113/219 (51%), Positives = 156/219 (71%)
Frame = -3 / +1
Query: 997 DFVTNVQIRGITLLNSKFFHLNIFECKNVLIDKVTVKAPGDSPNTDGIHIGDSSNVTISS 818
+FV N +RG+T +SK FH+ +F CKN+ D T+ APGDSPNTDGIH+G S++V I +
Sbjct: 487 NFVNNSIVRGVTSKDSKNFHVMVFGCKNITFDGFTITAPGDSPNTDGIHMGKSTDVKILN 666
Query: 817 TTIGVGDDCISIGPGSKMIRIHGVKCGPGHGISVGSLGRYKDEKDVEDVQVTGCTIAGTT 638
T IG GDDC+SIG GSK I + GV CGPGHG+SVGSLG++ E++VE + V CT+ T
Sbjct: 667 TNIGTGDDCVSIGDGSKQITVQGVNCGPGHGLSVGSLGKFTTEENVEGITVKNCTLTATD 846
Query: 637 NGLRIKSYEDSKSSLKASKFLYEGITMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVV 458
NG+RIK++ D+ ++ S +E ITM NV P+IIDQ+Y P N C K SK+ ++ +
Sbjct: 847 NGVRIKTWPDAPGTITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCSKKNPSKIKLSKIS 1026
Query: 457 FKNIHGTSNTPEAITLNCANNLPCQGVQLVNVDIKYNGS 341
FKN+ GTS T E + L C++ +PC GV+L NVD+K+NG+
Sbjct: 1027 FKNVKGTSGTAEGVVLICSSAVPCDGVELNNVDLKFNGA 1143
Score = 98.2 bits (208), Expect(3) = 3e-036
Identities = 45/94 (47%), Positives = 51/94 (54%)
Frame = +3 / -3
Query: 726 PWPGPHLTPWMRIILLPGPMEMQSSXXXXXXXXXXXXXESPMWMPSVLGLSPGALTVTLS 905
P PGP TPW I LLP P+E QSS + PMW+PSVLGLSPGA+ V S
Sbjct: 758 P*PGPQFTPWTVICLLPSPIETQSSPVPMLVLRILTSVDFPMWIPSVLGLSPGAVIVKPS 579
Query: 906 ISTFLHSKMLRWKNLLLSSVMPRIWTFVTKSSTK 1007
FLH K + WK LL V PR +TK K
Sbjct: 578 NVIFLHPKTITWKFLLSLEVTPRTMELLTKLKPK 477
Score = 68.4 bits (143), Expect(2) = 4e-093
Identities = 28/64 (43%), Positives = 36/64 (56%)
Frame = -3 / +1
Query: 1342 GDGKSDSTPMILKAWKNACEATGVQKIVIPPGNYLTGGLELKGPCKSSIIIRLDGNLLGT 1163
G SD + AW AC +T KIVIP G Y G+ELKGPCK+ I +++DG +
Sbjct: 124 GKPNSDIGQALTSAWNEACASTTAAKIVIPAGTYQLNGIELKGPCKAPIELQVDGTIQAP 303
Query: 1162 GDLS 1151
D S
Sbjct: 304 ADPS 315
Score = 57.4 bits (119), Expect(3) = 3e-036
Identities = 33/76 (43%), Positives = 36/76 (47%)
Frame = +3 / -3
Query: 345 PLYLMSTLTSCTPWHGRLLAQLSVMASGVLEVPWMFLKTTSLTATLEAPDLTQMLLGQYF 524
PL L STL S TP HG L Q+ S V EVP FL L + L G Y
Sbjct: 1139 PLNLRSTLFSSTPSHGTALLQIRTTPSAVPEVPLTFLNEILLNFIFDGFFLEHWFHG*YS 960
Query: 525 WSMMMG*ETLSMVMPS 572
WSMM G T MV+ S
Sbjct: 959 WSMMTGFLTFVMVISS 912
Score = 37.7 bits (76), Expect(3) = 3e-036
Identities = 18/34 (52%), Positives = 21/34 (61%)
Frame = +3 / -3
Query: 1206 LQGPFSSSPPVR*LPGGMTIFCTPVASHAFFHAL 1307
LQGP SS P +P G+TIF V +HA F AL
Sbjct: 260 LQGPLSSIPFNWYVPAGITIFAAVVDAHASFQAL 159
>emb|Y11118.1|MSPOLGAL3 M.sativa PG3 gene
Length = 2185
Score = 202 bits (435), Expect(4) = 2e-078
Identities = 84/168 (50%), Positives = 110/168 (65%)
Frame = -3 / +1
Query: 997 DFVTNVQIRGITLLNSKFFHLNIFECKNVLIDKVTVKAPGDSPNTDGIHIGDSSNVTISS 818
+F+ + +R IT +SK FH+N+ C N D VT+ AP S NTDGIHIG S++V + +
Sbjct: 1201 NFLKHSIVRDITSKDSKNFHVNVLGCTNFTFDSVTITAPVTSINTDGIHIGRSTDVKVLN 1380
Query: 817 TTIGVGDDCISIGPGSKMIRIHGVKCGPGHGISVGSLGRYKDEKDVEDVQVTGCTIAGTT 638
T I GDDC+S+G GS+ I + V CGPGHGISVGSLG+Y E+ VE V V CTI T
Sbjct: 1381 TNIATGDDCVSLGDGSRQITVQNVNCGPGHGISVGSLGKYPKEEAVEHVLVKNCTITNTD 1560
Query: 637 NGLRIKSYEDSKSSLKASKFLYEGITMDNVSYPIIIDQKYCPNNICVK 494
NG+RIK++ S + + +E I M NV P+IIDQ+YCP N C K
Sbjct: 1561 NGVRIKTWPSSPGTSPITDMHFEDIIMVNVLNPVIIDQEYCPWNQCSK 1704
Score = 68.9 bits (144), Expect(4) = 8e-023
Identities = 36/84 (42%), Positives = 41/84 (48%)
Frame = +3 / -3
Query: 723 MPWPGPHLTPWMRIILLPGPMEMQSSXXXXXXXXXXXXXESPMWMPSVLGLSPGALTVTL 902
+PWPGP T W I LLP P E QSS + PM +PSV L GA+ VTL
Sbjct: 1475 IPWPGPQFTFWTVICLLPSPNETQSSPVAMFVLRTFTSVDLPMCIPSVFMLVTGAVMVTL 1296
Query: 903 SISTFLHSKMLRWKNLLLSSVMPR 974
S + K L K LL VM R
Sbjct: 1295 SNVKLVQPKTLT*KFLLSFEVMSR 1224
Score = 60.6 bits (126), Expect(4) = 2e-078
Identities = 21/48 (43%), Positives = 33/48 (68%)
Frame = -3 / +2
Query: 484 SKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLVNVDIKYNGS 341
SK+ ++ V+FKNI GTS T + + L C+ + PC V+L NV + +NG+
Sbjct: 1808 SKIKISKVIFKNIRGTSKTKDGVVLICSKSFPCDAVELNNVALTFNGA 1951
Score = 54.2 bits (112), Expect(4) = 2e-078
Identities = 19/44 (43%), Positives = 30/44 (68%)
Frame = -3 / +3
Query: 1303 AWKNACEATGVQKIVIPPGNYLTGGLELKGPCKSSIIIRLDGNL 1172
AWK AC +T K++IP G Y +++KGPCK+ I +++DG +
Sbjct: 462 AWKAACGSTTASKVMIPRGVYKMHAVDVKGPCKAPIEVQVDGTI 593
Score = 42.8 bits (87), Expect(4) = 8e-023
Identities = 21/42 (50%), Positives = 25/42 (59%)
Frame = +3 / -2
Query: 345 PLYLMSTLTSCTPWHGRLLAQLSVMASGVLEVPWMFLKTTSL 470
PL + +TL S T HG+LL + S V EVP MFLK T L
Sbjct: 1947 PLNVRATLFSSTASHGKLLLHIRTTPSLVFEVPLMFLKITLL 1822
Score = 40.9 bits (83), Expect(4) = 2e-078
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = -3 / +3
Query: 1135 WIEIENVENLSINGHGTIDGQGALVWSKNQCQHSYNCK 1022
WI+ V+ +++G G DG G W +N C + CK
Sbjct: 636 WIKFGYVDYFTLSGKGVFDGNGETAWRQNDCGKNSTCK 749
Score = 30.4 bits (60), Expect(4) = 8e-023
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +3 / -3
Query: 513 GQYFWSMMMG*ETLSMVMPS 572
GQY WSM+ G TL+M++ S
Sbjct: 1685 GQYSWSMITGFRTLTMIISS 1626
Score = 26.7 bits (52), Expect(4) = 8e-023
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = +3 / -1
Query: 1206 LQGPFSSSPPVR*LPGGMTIFCTPVASHAFFHA 1304
LQGPF+S+ + P G+ V HA F A
Sbjct: 559 LQGPFTSTACILYTPLGIITLLAVVDPHAAFQA 461
>gb|AY219848.1| Medicago sativa polygalactorunase PG11 precursor, gene, complete cds
Length = 2213
Score = 221 bits (477), Expect(3) = 3e-074
Identities = 89/180 (49%), Positives = 120/180 (66%)
Frame = -3 / +1
Query: 994 FVTNVQIRGITLLNSKFFHLNIFECKNVLIDKVTVKAPGDSPNTDGIHIGDSSNVTISST 815
FV N + G+T +SK FH+ + C N D TV APGDSPNTDGIH+G S+++ I +T
Sbjct: 1225 FVNNSMVHGLTSKDSKNFHVMVLGCNNFTFDGFTVTAPGDSPNTDGIHMGRSTDIKILNT 1404
Query: 814 TIGVGDDCISIGPGSKMIRIHGVKCGPGHGISVGSLGRYKDEKDVEDVQVTGCTIAGTTN 635
I GDDCIS+G GS+ I + VKCGPGHGISVGSLG+Y E +VE V V CT+ T N
Sbjct: 1405 NIATGDDCISLGDGSRKITVQNVKCGPGHGISVGSLGKYTTEDNVEGVIVKNCTLTATQN 1584
Query: 634 GLRIKSYEDSKSSLKASKFLYEGITMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVVF 455
G+RIK++ D+ ++ S +E I M+NV P+IIDQ+YCP N C K ++ ++ V+
Sbjct: 1585 GVRIKTWPDAPGTITVSDIHFEDIIMNNVMNPVIIDQEYCPWNQCSKKVITQELISCFVY 1764
Score = 92.7 bits (196), Expect(3) = 5e-027
Identities = 49/129 (37%), Positives = 58/129 (44%)
Frame = +3 / -1
Query: 621 LMRRPFVVPAIVHPVTCXXXXXXXXXXXXXXXXXMPWPGPHLTPWMRIILLPGPMEMQSS 800
L+ PF V V +T +P PGPH T W I LLP P E+QSS
Sbjct: 1598 LILTPF*VAVRVQFLTITPSTLSSVVYFPRLPTLIP*PGPHFTFWTVIFLLPSPNEIQSS 1419
Query: 801 XXXXXXXXXXXXXESPMWMPSVLGLSPGALTVTLSISTFLHSKMLRWKNLLLSSVMPRIW 980
+ PMW+PSVLGLSPGA+TV S LH K + WK LL V P
Sbjct: 1418 PVAMLVLRILISVDLPMWIPSVLGLSPGAVTVKPSNVKLLHPKTITWKFLLSLEVRPWTM 1239
Query: 981 TFVTKSSTK 1007
+T K
Sbjct: 1238 ELLTNIKPK 1212
Score = 64.3 bits (134), Expect(3) = 3e-074
Identities = 25/51 (49%), Positives = 33/51 (64%)
Frame = -3 / +2
Query: 1303 AWKNACEATGVQKIVIPPGNYLTGGLELKGPCKSSIIIRLDGNLLGTGDLS 1151
AWK AC +T KI+IP G Y G+ELKGPCK+ I +++DG + D S
Sbjct: 785 AWKEACLSTTAAKIMIPGGTYRMNGVELKGPCKAPIEVQVDGTIQAPADPS 937
Score = 40.5 bits (82), Expect(3) = 5e-027
Identities = 20/37 (54%), Positives = 22/37 (59%)
Frame = +3 / -2
Query: 345 PLYLMSTLTSCTPWHGRLLAQLSVMASGVLEVPWMFL 455
PL + TL S TP HG L Q+ SGV EVP MFL
Sbjct: 1990 PLNVRPTLFSSTPSHGTPLLQIRTTPSGVPEVP*MFL 1880
Score = 36.8 bits (74), Expect(3) = 3e-074
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = -3 / +2
Query: 1135 WIEIENVENLSINGHGTIDGQGALVWSK 1052
WI+ +E+L+++G G DGQGA V+ K
Sbjct: 959 WIKFLYMEHLTLSGKGVFDGQGATVYKK 1042
Score = 29.9 bits (59), Expect(3) = 5e-027
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +3 / -1
Query: 513 GQYFWSMMMG*ETLSMVMPS 572
GQY WSMM G TL M++ S
Sbjct: 1706 GQYSWSMMTGFMTLFMIISS 1647
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,875,801
Number of Sequences: 7669
Number of extensions: 105724
Number of successful extensions: 1103
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 429143172
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)