TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115184.2.1
(642 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U20431.1|MSU20431 Medicago sativa polygalacturonase mRNA... 160 2e-040
emb|Y11118.1|MSPOLGAL3 M.sativa PG3 gene 100 2e-033
gb|AY219848.1| Medicago sativa polygalactorunase PG11 precu... 105 5e-024
>gb|U20431.1|MSU20431 Medicago sativa polygalacturonase mRNA, complete cds
Length = 1492
Score = 160 bits (343), Expect = 2e-040
Identities = 60/129 (46%), Positives = 90/129 (69%)
Frame = +3 / +1
Query: 3 GHGISVGSLGRYKDEKDVEDVKVTGCTLAGTTNGLRIKSYEDSKSSLKATKFLYQDVTMD 182
GHG+SVGSLG++ E++VE + V CTL T NG+RIK++ D+ ++ + ++D+TM
Sbjct: 751 GHGLSVGSLGKFTTEENVEGITVKNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDITMT 930
Query: 183 NVSYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQ 362
NV P+IIDQ+Y P N C K SK+ ++ + FKN+ GTS T E + L C++ +PC GV+
Sbjct: 931 NVKNPVIIDQEYYPWNQCSKKNPSKIKLSKISFKNVKGTSGTAEGVVLICSSAVPCDGVE 1110
Query: 363 LINVDIKYN 389
L NVD+K+N
Sbjct: 1111 LNNVDLKFN 1137
>emb|Y11118.1|MSPOLGAL3 M.sativa PG3 gene
Length = 2185
Score = 99.6 bits (211), Expect(2) = 2e-033
Identities = 41/80 (51%), Positives = 53/80 (66%)
Frame = +3 / +1
Query: 3 GHGISVGSLGRYKDEKDVEDVKVTGCTLAGTTNGLRIKSYEDSKSSLKATKFLYQDVTMD 182
GHGISVGSLG+Y E+ VE V V CT+ T NG+RIK++ S + T ++D+ M
Sbjct: 1465 GHGISVGSLGKYPKEEAVEHVLVKNCTITNTDNGVRIKTWPSSPGTSPITDMHFEDIIMV 1644
Query: 183 NVSYPIIIDQKYCPNNICVK 242
NV P+IIDQ+YCP N C K
Sbjct: 1645 NVLNPVIIDQEYCPWNQCSK 1704
Score = 57.4 bits (119), Expect(2) = 2e-033
Identities = 20/46 (43%), Positives = 31/46 (67%)
Frame = +3 / +2
Query: 252 SKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLINVDIKYN 389
SK+ ++ V+FKNI GTS T + + L C+ + PC V+L NV + +N
Sbjct: 1808 SKIKISKVIFKNIRGTSKTKDGVVLICSKSFPCDAVELNNVALTFN 1945
>gb|AY219848.1| Medicago sativa polygalactorunase PG11 precursor, gene, complete cds
Length = 2213
Score = 105 bits (224), Expect = 5e-024
Identities = 42/93 (45%), Positives = 62/93 (66%)
Frame = +3 / +1
Query: 3 GHGISVGSLGRYKDEKDVEDVKVTGCTLAGTTNGLRIKSYEDSKSSLKATKFLYQDVTMD 182
GHGISVGSLG+Y E +VE V V CTL T NG+RIK++ D+ ++ + ++D+ M+
Sbjct: 1486 GHGISVGSLGKYTTEDNVEGVIVKNCTLTATQNGVRIKTWPDAPGTITVSDIHFEDIIMN 1665
Query: 183 NVSYPIIIDQKYCPNNICVKSGASKVAVNDVVF 281
NV P+IIDQ+YCP N C K ++ ++ V+
Sbjct: 1666 NVMNPVIIDQEYCPWNQCSKKVITQELISCFVY 1764
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 3,375,723
Number of Sequences: 7669
Number of extensions: 29860
Number of successful extensions: 629
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 158426370
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)