TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115079.2.1
(636 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|X68106.1|MSCHSI M.sativa msCHSI mRNA for chalcone synthase 85 1e-023
gb|U01019.1|U01019 Medicago sativa clone MsCHS4-2 chalcone ... 85 1e-023
emb|X68107.1|MSCHSII M.sativa msCHSII mRNA for chalcone syn... 85 1e-023
gb|U01021.1|U01021 Medicago sativa clone MsCHS12-1 chalcone... 84 2e-023
gb|L02903.1|ALFCHS4A Medicago sativa chalcone synthase (CHS... 84 2e-023
gb|L02902.1|ALFCHS2A Medicago sativa chalcone synthase (CHS... 83 3e-022
gb|L02904.1|ALFCHS8A Medicago sativa chalcone synthase (CHS... 84 1e-021
gb|DQ122840.1| Medicago sativa clone K9 chalcone synthase m... 84 3e-021
gb|U01018.1|U01018 Medicago sativa clone MsCHS4-1 chalcone ... 83 5e-021
gb|L02905.1|ALFCHS9A Medicago sativa chalcone synthase (CHS... 83 5e-021
gb|L02901.1|ALFCHS1A Medicago sativa chalcone synthase (CHS... 83 5e-021
gb|U01020.1|U01020 Medicago sativa clone MsCHS6-4 chalcone ... 83 5e-021
>emb|X68106.1|MSCHSI M.sativa msCHSI mRNA for chalcone synthase
Length = 1361
Score = 85.4 bits (180), Expect(2) = 1e-023
Identities = 31/61 (50%), Positives = 46/61 (75%)
Frame = -2 / +2
Query: 578 VGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKALMNYGNVSSNTIFYVLEYL 399
+GI D+N IFW HPGGPAIL+++E L L+PEK+K +R+ L YGN+SS + ++L+ +
Sbjct: 938 LGISDYNSIFWIAHPGGPAILDQVEQKLALKPEKMKATREVLSEYGNMSSACVLFILDEM 1117
Query: 398 R 396
R
Sbjct: 1118 R 1120
Score = 38.2 bits (77), Expect(2) = 1e-023
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = -2 / +2
Query: 398 RDELKKGAISEEWGLILAFGPGITFEGLLVRGV 300
+D LK EWG++ FGPG+T E +++R V
Sbjct: 1133 QDGLKTTGEGLEWGVLFGFGPGLTIETVVLRSV 1231
>gb|U01019.1|U01019 Medicago sativa clone MsCHS4-2 chalcone synthase mRNA, partial cds
Length = 1268
Score = 85.4 bits (180), Expect(2) = 1e-023
Identities = 31/61 (50%), Positives = 46/61 (75%)
Frame = -2 / +1
Query: 578 VGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKALMNYGNVSSNTIFYVLEYL 399
+GI D+N IFW HPGGPAIL+++E L L+PEK+K +R+ L YGN+SS + ++L+ +
Sbjct: 820 LGISDYNSIFWIAHPGGPAILDQVEQKLALKPEKMKATREVLSEYGNMSSACVLFILDEM 999
Query: 398 R 396
R
Sbjct: 1000 R 1002
Score = 38.2 bits (77), Expect(2) = 1e-023
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = -2 / +1
Query: 398 RDELKKGAISEEWGLILAFGPGITFEGLLVRGV 300
+D LK EWG++ FGPG+T E +++R V
Sbjct: 1015 QDGLKTTGEGLEWGVLFGFGPGLTIETVVLRSV 1113
>emb|X68107.1|MSCHSII M.sativa msCHSII mRNA for chalcone synthase
Length = 932
Score = 85.4 bits (180), Expect(2) = 1e-023
Identities = 31/61 (50%), Positives = 46/61 (75%)
Frame = -2 / +2
Query: 578 VGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKALMNYGNVSSNTIFYVLEYL 399
+GI D+N IFW HPGGPAIL+++E L L+PEK+K +R+ L YGN+SS + ++L+ +
Sbjct: 497 LGISDYNSIFWIAHPGGPAILDQVEQKLALKPEKMKATREVLSEYGNMSSACVLFILDEM 676
Query: 398 R 396
R
Sbjct: 677 R 679
Score = 38.2 bits (77), Expect(2) = 1e-023
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = -2 / +2
Query: 398 RDELKKGAISEEWGLILAFGPGITFEGLLVRGV 300
+D LK EWG++ FGPG+T E +++R V
Sbjct: 692 QDGLKTTGEGLEWGVLFGFGPGLTIETVVLRSV 790
>gb|U01021.1|U01021 Medicago sativa clone MsCHS12-1 chalcone synthase mRNA, complete cds
Length = 1380
Score = 84.5 bits (178), Expect(2) = 2e-023
Identities = 32/80 (40%), Positives = 51/80 (63%)
Frame = -2 / +1
Query: 635 LPEKIESRIEGFCRTLMNQVGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKA 456
+P + I+ +GI D+N IFW HPGGPAIL+++E L L+PEK++ +R+
Sbjct: 877 VPGIVSKNIDKALVEAFQPLGISDYNSIFWIAHPGGPAILDQVEQKLALKPEKMRATREV 1056
Query: 455 LMNYGNVSSNTIFYVLEYLR 396
L YGN+SS + ++L+ +R
Sbjct: 1057 LSEYGNMSSACVLFILDEMR 1116
Score = 38.2 bits (77), Expect(2) = 2e-023
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = -2 / +1
Query: 398 RDELKKGAISEEWGLILAFGPGITFEGLLVRGV 300
+D LK EWG++ FGPG+T E +++R V
Sbjct: 1129 QDGLKTTGEGLEWGVLFGFGPGLTIETVVLRSV 1227
>gb|L02903.1|ALFCHS4A Medicago sativa chalcone synthase (CHS4) mRNA, complete cds
Length = 1281
Score = 84.5 bits (178), Expect(2) = 2e-023
Identities = 32/80 (40%), Positives = 51/80 (63%)
Frame = -2 / +3
Query: 635 LPEKIESRIEGFCRTLMNQVGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKA 456
+P + I+ +GI D+N IFW HPGGPAIL+++E L L+PEK++ +R+
Sbjct: 795 VPGIVSKNIDKALVEAFQPLGISDYNSIFWIAHPGGPAILDQVEQKLALKPEKMRATREV 974
Query: 455 LMNYGNVSSNTIFYVLEYLR 396
L YGN+SS + ++L+ +R
Sbjct: 975 LSEYGNMSSACVLFILDEMR 1034
Score = 38.2 bits (77), Expect(2) = 2e-023
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = -2 / +3
Query: 398 RDELKKGAISEEWGLILAFGPGITFEGLLVRGV 300
+D LK EWG++ FGPG+T E +++R V
Sbjct: 1047 QDGLKTTGEGLEWGVLFGFGPGLTIETVVLRSV 1145
>gb|L02902.1|ALFCHS2A Medicago sativa chalcone synthase (CHS2) mRNA, complete cds
Length = 1254
Score = 83.1 bits (175), Expect(2) = 3e-022
Identities = 30/61 (49%), Positives = 45/61 (73%)
Frame = -2 / +2
Query: 578 VGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKALMNYGNVSSNTIFYVLEYL 399
+GI D+N IFW HPGGPAIL+++E L L+PEK+ +R+ L YGN+SS + ++L+ +
Sbjct: 926 LGISDYNSIFWIAHPGGPAILDQVEQKLALKPEKMNATREVLSEYGNMSSACVLFILDEM 1105
Query: 398 R 396
R
Sbjct: 1106 R 1108
Score = 35.9 bits (72), Expect(2) = 3e-022
Identities = 11/22 (50%), Positives = 17/22 (77%)
Frame = -2 / +2
Query: 365 EWGLILAFGPGITFEGLLVRGV 300
EWG++ FGPG+T E +++R V
Sbjct: 1154 EWGVLFGFGPGLTIETVVLRSV 1219
>gb|L02904.1|ALFCHS8A Medicago sativa chalcone synthase (CHS8) mRNA, complete cds
Length = 1347
Score = 83.5 bits (176), Expect(2) = 1e-021
Identities = 31/61 (50%), Positives = 45/61 (73%)
Frame = -2 / +1
Query: 578 VGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKALMNYGNVSSNTIFYVLEYL 399
+GI D+N IFW HPGGPAIL+++E L L+PEK+K +R+ L YGN+SS + +L+ +
Sbjct: 910 LGISDYNSIFWIAHPGGPAILDQVEQKLALKPEKMKATREVLSEYGNMSSACVLVILDEM 1089
Query: 398 R 396
R
Sbjct: 1090 R 1092
Score = 33.6 bits (67), Expect(2) = 1e-021
Identities = 13/33 (39%), Positives = 21/33 (63%)
Frame = -2 / +1
Query: 398 RDELKKGAISEEWGLILAFGPGITFEGLLVRGV 300
+D LK E+G++ FGPG+T E +++R V
Sbjct: 1105 QDGLKTTGEGLEFGVLFGFGPGLTIETVVLRSV 1203
>gb|DQ122840.1| Medicago sativa clone K9 chalcone synthase mRNA, partial cds
Length = 660
Score = 84.0 bits (177), Expect(2) = 3e-021
Identities = 33/88 (37%), Positives = 53/88 (60%)
Frame = -2 / +2
Query: 635 LPEKIESRIEGFCRTLMNQVGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKA 456
+P + I+ + I D+N IFW HPGGPAIL+++E L L+PEK+K +R+
Sbjct: 335 VPGIVSKNIDKALVEAFQPLNISDYNSIFWIAHPGGPAILDQVEQKLGLKPEKMKATREV 514
Query: 455 LMNYGNVSSNTIFYVLEYLRDELKKGAI 372
L YGN+SS + ++L+ +R + K +
Sbjct: 515 LSEYGNMSSACVLFILDEMRKKSTKDGL 598
Score = 31.8 bits (63), Expect(2) = 3e-021
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -2 / +2
Query: 398 RDELKKGAISEEWGLILAFGPGIT 327
+D LK EWG++ FGPG+T
Sbjct: 587 KDGLKTTGEGLEWGVLFGFGPGLT 658
>gb|U01018.1|U01018 Medicago sativa clone MsCHS4-1 chalcone synthase mRNA, complete cds
Length = 1423
Score = 82.6 bits (174), Expect(2) = 5e-021
Identities = 32/80 (40%), Positives = 50/80 (62%)
Frame = -2 / +1
Query: 635 LPEKIESRIEGFCRTLMNQVGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKA 456
+P + I+ + I D+N IFW HPGGPAIL+++E L L+PEK+K +R+
Sbjct: 916 VPGIVSKNIDKALIEAFQPLNISDYNSIFWIAHPGGPAILDQVEEKLGLKPEKMKATREV 1095
Query: 455 LMNYGNVSSNTIFYVLEYLR 396
L YGN+SS + ++L+ +R
Sbjct: 1096 LSEYGNMSSACVLFILDEMR 1155
Score = 32.2 bits (64), Expect(2) = 5e-021
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = -2 / +1
Query: 365 EWGLILAFGPGITFEGLLVRGV 300
+WG++ FGPG+T E +++ V
Sbjct: 1201 DWGVLFGFGPGLTIETVVLHSV 1266
>gb|L02905.1|ALFCHS9A Medicago sativa chalcone synthase (CHS9) mRNA, complete cds
Length = 1405
Score = 82.6 bits (174), Expect(2) = 5e-021
Identities = 32/80 (40%), Positives = 50/80 (62%)
Frame = -2 / +2
Query: 635 LPEKIESRIEGFCRTLMNQVGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKA 456
+P + I+ + I D+N IFW HPGGPAIL+++E L L+PEK+K +R+
Sbjct: 926 VPGIVSKNIDKALVEAFQPLNISDYNSIFWIAHPGGPAILDQVEQKLGLKPEKMKATREV 1105
Query: 455 LMNYGNVSSNTIFYVLEYLR 396
L YGN+SS + ++L+ +R
Sbjct: 1106 LSEYGNMSSACVLFILDEMR 1165
Score = 32.2 bits (64), Expect(2) = 5e-021
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = -2 / +2
Query: 365 EWGLILAFGPGITFEGLLVRGV 300
+WG++ FGPG+T E +++ V
Sbjct: 1211 DWGVLFGFGPGLTIETVVLHSV 1276
>gb|L02901.1|ALFCHS1A Medicago sativa chalcone synthase (CHS1) mRNA, complete cds
Length = 1386
Score = 82.6 bits (174), Expect(2) = 5e-021
Identities = 32/80 (40%), Positives = 50/80 (62%)
Frame = -2 / +2
Query: 635 LPEKIESRIEGFCRTLMNQVGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKA 456
+P + I+ + I D+N IFW HPGGPAIL+++E L L+PEK+K +R+
Sbjct: 896 VPGIVSKNIDKALIEAFQPLNISDYNSIFWIAHPGGPAILDQVEEKLGLKPEKMKATREV 1075
Query: 455 LMNYGNVSSNTIFYVLEYLR 396
L YGN+SS + ++L+ +R
Sbjct: 1076 LSEYGNMSSACVLFILDEMR 1135
Score = 32.2 bits (64), Expect(2) = 5e-021
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = -2 / +2
Query: 365 EWGLILAFGPGITFEGLLVRGV 300
+WG++ FGPG+T E +++ V
Sbjct: 1181 DWGVLFGFGPGLTIETVVLHSV 1246
>gb|U01020.1|U01020 Medicago sativa clone MsCHS6-4 chalcone synthase mRNA, partial cds
Length = 1009
Score = 82.6 bits (174), Expect(2) = 5e-021
Identities = 32/80 (40%), Positives = 50/80 (62%)
Frame = -2 / +1
Query: 635 LPEKIESRIEGFCRTLMNQVGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKA 456
+P + I+ + I D+N IFW HPGGPAIL+++E L L+PEK+K +R+
Sbjct: 508 VPGIVSKNIDKALVEAFQPLNISDYNSIFWIAHPGGPAILDQVEQKLGLKPEKMKATREV 687
Query: 455 LMNYGNVSSNTIFYVLEYLR 396
L YGN+SS + ++L+ +R
Sbjct: 688 LSEYGNMSSACVLFILDEMR 747
Score = 32.2 bits (64), Expect(2) = 5e-021
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = -2 / +1
Query: 365 EWGLILAFGPGITFEGLLVRGV 300
+WG++ FGPG+T E +++ V
Sbjct: 793 DWGVLFGFGPGLTIETVVLHSV 858
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,196,935
Number of Sequences: 7669
Number of extensions: 87230
Number of successful extensions: 1315
Number of sequences better than 1.0e-020: 12
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 156573430
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)