TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3089980.2.1
(540 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF201458.1|AF201458 Medicago sativa isoflavone reductase... 122 3e-029
emb|X58078.1|MSISREDMR M.sativa mRNA for isoflavone reductase 91 4e-024
>gb|AF201458.1|AF201458 Medicago sativa isoflavone reductase-like NAD(P)H-dependent
oxidoreductase (IRL1) mRNA, complete cds
Length = 1220
Score = 122 bits (262), Expect = 3e-029
Identities = 52/131 (39%), Positives = 77/131 (58%)
Frame = -2 / +2
Query: 539 RATVFGDDATRAIFVHEADMAAVTVRAVDDPRALDKTLYLRPPANTCSLAHLVRLWEDKT 360
+ ++GD +A+F E D+ T+RAVD P +K LY++PP + S LV LWE K+
Sbjct: 551 KVVIYGDGNPKAVFNKEDDIGTFTIRAVDAPGPWNKILYIKPPKESYSSNELVALWEKKS 730
Query: 359 GRALDKYYMPDEELVNRIRDSPLPLNFQLAMVHATVVAGVCDQTVDAEAGGVEATELYPD 180
G+ K +P++ L+ I+++P+P+ L + H V G V + GVEA ELYPD
Sbjct: 731 GKPAQKPSLPEDNLLKDIQEAPIPITVVLPIHHGVFVKGDHTNFVIEPSFGVEAYELYPD 910
Query: 179 VNYVTVHDYLD 147
V Y TV +YLD
Sbjct: 911 VKYTTVEEYLD 943
>emb|X58078.1|MSISREDMR M.sativa mRNA for isoflavone reductase
Length = 1181
Score = 91.3 bits (193), Expect(2) = 4e-024
Identities = 34/106 (32%), Positives = 62/106 (58%)
Frame = -2 / +1
Query: 539 RATVFGDDATRAIFVHEADMAAVTVRAVDDPRALDKTLYLRPPANTCSLAHLVRLWEDKT 360
+ + GD + +V EAD+ T+RA +DP L+K +++R P N + ++ LWE K
Sbjct: 607 KVVILGDGNVKGAYVTEADVGTFTIRAANDPNTLNKAVHIRLPKNYLTQNEVIALWEKKI 786
Query: 359 GRALDKYYMPDEELVNRIRDSPLPLNFQLAMVHATVVAGVCDQTVD 222
G+ L+K Y+ +E+++ I++S P N+ LA+ H+ + G +D
Sbjct: 787 GKTLEKTYVSEEQVLKDIQESSFPHNYLLALYHSQQIKGDAVYEID 924
Score = 33.6 bits (67), Expect(2) = 4e-024
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = -2 / +1
Query: 215 AGGVEATELYPDVNYVTVHDYLD 147
A +EA+E YPDV Y T +YL+
Sbjct: 928 AKDIEASEAYPDVTYTTADEYLN 996
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 2,333,809
Number of Sequences: 7669
Number of extensions: 20898
Number of successful extensions: 768
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 41
effective length of database: 934,139
effective search space used: 128911182
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)