TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3071418.2.1
(1432 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF000976.1|MSAF000976 Medicago sativa 7-O-methyltransfer... 179 7e-049
gb|U97125.1|MSU97125 Medicago sativa isoflavone-O-methytran... 179 8e-046
gb|AF000975.1|MSAF000975 Medicago sativa 7-O-methyltransfer... 179 8e-046
emb|AX050436.1| Sequence 1 from Patent WO0071736 179 8e-046
gb|AF023481.1|AF023481 Medicago sativa o-methytransferase (... 97 1e-037
gb|M63853.1|ALFSALMCAT Medicago sativa S-adenosyl-L-methion... 98 2e-021
emb|AX259373.1| Sequence 3 from Patent WO0173090 98 2e-021
>gb|AF000976.1|MSAF000976 Medicago sativa 7-O-methyltransferase (7-IOMT(9)) mRNA, complete cds
Length = 1227
Score = 179 bits (386), Expect(2) = 7e-049
Identities = 74/175 (42%), Positives = 108/175 (61%)
Frame = -1 / +3
Query: 754 LMPIVLRECGETFRGIDSLVDVXXXXXXXXXXXXXAFPHLKCSVLDLPHVVAGAPSDGNV 575
L+ + LR+C F G++S+VDV FP LKC V D P VV N+
Sbjct: 543 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 722
Query: 574 QFVAGNMFESIPPATAVFLKKTLHDWGDDECVKILKNCKQAISPRDAGGKVIILDVVVGY 395
+V G+MF SIP A AV LK LH+W D +C++ILK CK+A++ GKV I+D+V+
Sbjct: 723 TYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVINE 902
Query: 394 KQSNIKHQETQVMFDLYMMAVNGVERDEQEWKKIFTEAGFKDYKILPVIGDVSVI 230
K+ + + +++ D+ M +NG ER+E+EWKK+F EAGF+ YKI P+ G +S+I
Sbjct: 903 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLI 1067
Score = 34.1 bits (68), Expect(2) = 7e-049
Identities = 15/49 (30%), Positives = 25/49 (51%)
Frame = -1 / +3
Query: 1342 MALIMQESSSQDLLQAHDELLHHSLCFAKSLALAVALDLRIPDAIHHHG 1196
MA + ++ +A L H F S++L A+ + IP+ IH+HG
Sbjct: 24 MASSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVGMNIPNIIHNHG 170
>gb|U97125.1|MSU97125 Medicago sativa isoflavone-O-methytransferase mRNA, complete cds
Length = 1231
Score = 179 bits (385), Expect = 8e-046
Identities = 74/175 (42%), Positives = 108/175 (61%)
Frame = -1 / +1
Query: 754 LMPIVLRECGETFRGIDSLVDVXXXXXXXXXXXXXAFPHLKCSVLDLPHVVAGAPSDGNV 575
L+ + LR+C F G++S+VDV FP LKC V D P VV N+
Sbjct: 544 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 723
Query: 574 QFVAGNMFESIPPATAVFLKKTLHDWGDDECVKILKNCKQAISPRDAGGKVIILDVVVGY 395
+V G+MF SIP A AV LK LH+W D +C++ILK CK+A++ GKV I+D+V+
Sbjct: 724 TYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIDK 903
Query: 394 KQSNIKHQETQVMFDLYMMAVNGVERDEQEWKKIFTEAGFKDYKILPVIGDVSVI 230
K+ + + +++ D+ M +NG ER+E+EWKK+F EAGF+ YKI P+ G +S+I
Sbjct: 904 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLI 1068
>gb|AF000975.1|MSAF000975 Medicago sativa 7-O-methyltransferase (7-IOMT(6)) mRNA, complete cds
Length = 1284
Score = 179 bits (385), Expect = 8e-046
Identities = 74/175 (42%), Positives = 108/175 (61%)
Frame = -1 / +3
Query: 754 LMPIVLRECGETFRGIDSLVDVXXXXXXXXXXXXXAFPHLKCSVLDLPHVVAGAPSDGNV 575
L+ + LR+C F G++S+VDV FP LKC V D P VV N+
Sbjct: 549 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 728
Query: 574 QFVAGNMFESIPPATAVFLKKTLHDWGDDECVKILKNCKQAISPRDAGGKVIILDVVVGY 395
+V G+MF SIP A AV LK LH+W D +C++ILK CK+A++ GKV I+D+V+
Sbjct: 729 TYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIDE 908
Query: 394 KQSNIKHQETQVMFDLYMMAVNGVERDEQEWKKIFTEAGFKDYKILPVIGDVSVI 230
K+ + + +++ D+ M +NG ER+E+EWKK+F EAGF+ YKI P+ G +S+I
Sbjct: 909 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLI 1073
>emb|AX050436.1| Sequence 1 from Patent WO0071736
Length = 1231
Score = 179 bits (385), Expect = 8e-046
Identities = 74/175 (42%), Positives = 108/175 (61%)
Frame = -1 / +1
Query: 754 LMPIVLRECGETFRGIDSLVDVXXXXXXXXXXXXXAFPHLKCSVLDLPHVVAGAPSDGNV 575
L+ + LR+C F G++S+VDV FP LKC V D P VV N+
Sbjct: 544 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 723
Query: 574 QFVAGNMFESIPPATAVFLKKTLHDWGDDECVKILKNCKQAISPRDAGGKVIILDVVVGY 395
+V G+MF SIP A AV LK LH+W D +C++ILK CK+A++ GKV I+D+V+
Sbjct: 724 TYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIDK 903
Query: 394 KQSNIKHQETQVMFDLYMMAVNGVERDEQEWKKIFTEAGFKDYKILPVIGDVSVI 230
K+ + + +++ D+ M +NG ER+E+EWKK+F EAGF+ YKI P+ G +S+I
Sbjct: 904 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLI 1068
>gb|AF023481.1|AF023481 Medicago sativa o-methytransferase (iomt2003) mRNA, complete cds
Length = 1194
Score = 96.8 bits (205), Expect(2) = 1e-037
Identities = 37/86 (43%), Positives = 61/86 (70%)
Frame = -1 / +1
Query: 487 ECVKILKNCKQAISPRDAGGKVIILDVVVGYKQSNIKHQETQVMFDLYMMAVNGVERDEQ 308
+C++ILK CK+A++ GKV I+D+V+ K+ + + +++ D+ M +NG ER+E+
Sbjct: 787 DCLRILKKCKEAVTNDGKRGKVTIIDMVIDEKKDENQVTQIKLLMDVNMACLNGKERNEE 966
Query: 307 EWKKIFTEAGFKDYKILPVIGDVSVI 230
EWKK+F EAGF+ YKI P+ G +S+I
Sbjct: 967 EWKKLFIEAGFQHYKISPLTGFLSLI 1044
Score = 75.3 bits (158), Expect(2) = 1e-037
Identities = 34/83 (40%), Positives = 43/83 (51%)
Frame = -1 / +1
Query: 754 LMPIVLRECGETFRGIDSLVDVXXXXXXXXXXXXXAFPHLKCSVLDLPHVVAGAPSDGNV 575
L+ + LR+C F G++S+VDV FP LKC V D P VV N+
Sbjct: 547 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 726
Query: 574 QFVAGNMFESIPPATAVFLKKTL 506
+V G+MF SIP A AV LK L
Sbjct: 727 TYVGGDMFTSIPNADAVLLKDCL 795
>gb|M63853.1|ALFSALMCAT Medicago sativa S-adenosyl-L-methionine: caffeic acid
3-0-methyltransferase mRNA, complete cds
Length = 1317
Score = 98.2 bits (208), Expect = 2e-021
Identities = 41/90 (45%), Positives = 52/90 (57%)
Frame = -1 / +1
Query: 718 FRGIDSLVDVXXXXXXXXXXXXXAFPHLKCSVLDLPHVVAGAPSDGNVQFVAGNMFESIP 539
F G+ SLVDV +P +K DLPHV+ APS V+ V G+MF SIP
Sbjct: 622 FEGLKSLVDVGGGTGAVINTIVSKYPTIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSIP 801
Query: 538 PATAVFLKKTLHDWGDDECVKILKNCKQAI 449
A AVF+K HDW D+ C+K LKNC +A+
Sbjct: 802 KADAVFMKWICHDWSDEHCLKFLKNCYEAL 891
>emb|AX259373.1| Sequence 3 from Patent WO0173090
Length = 1098
Score = 98.2 bits (208), Expect = 2e-021
Identities = 41/90 (45%), Positives = 52/90 (57%)
Frame = -1 / +1
Query: 718 FRGIDSLVDVXXXXXXXXXXXXXAFPHLKCSVLDLPHVVAGAPSDGNVQFVAGNMFESIP 539
F G+ SLVDV +P +K DLPHV+ APS V+ V G+MF SIP
Sbjct: 592 FEGLKSLVDVGGGTGAVINTIVSKYPTIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSIP 771
Query: 538 PATAVFLKKTLHDWGDDECVKILKNCKQAI 449
A AVF+K HDW D+ C+K LKNC +A+
Sbjct: 772 KADAVFMKWICHDWSDEHCLKFLKNCYEAL 861
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 11,405,160
Number of Sequences: 7669
Number of extensions: 147740
Number of successful extensions: 928
Number of sequences better than 1.0e-020: 7
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 393609024
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)