TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3067450.2.1
(724 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF000976.1|MSAF000976 Medicago sativa 7-O-methyltransfer... 164 1e-041
gb|U97125.1|MSU97125 Medicago sativa isoflavone-O-methytran... 164 1e-041
gb|AF000975.1|MSAF000975 Medicago sativa 7-O-methyltransfer... 164 1e-041
emb|AX050436.1| Sequence 1 from Patent WO0071736 164 1e-041
gb|AF023481.1|AF023481 Medicago sativa o-methytransferase (... 88 4e-032
gb|M63853.1|ALFSALMCAT Medicago sativa S-adenosyl-L-methion... 100 1e-022
emb|AX259373.1| Sequence 3 from Patent WO0173090 100 1e-022
>gb|AF000976.1|MSAF000976 Medicago sativa 7-O-methyltransferase (7-IOMT(9)) mRNA, complete cds
Length = 1227
Score = 164 bits (353), Expect = 1e-041
Identities = 68/183 (37%), Positives = 106/183 (57%)
Frame = -2 / +3
Query: 720 LMDIAVRECGDVFQGIRSLVDVXXXXXXXXXXXXXXXXAIQCTVLDLAHVVATAPACAGL 541
L+++A+R+C VF G+ S+VDV ++C V D VV L
Sbjct: 543 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 722
Query: 540 SFVAGDMFEAIPPANAVFLKWIMHDWGDSECVTILKNCKKAIPPRDAGGKVIIVDTVVGA 361
++V GDMF +IP A+AV LK+I+H+W D +C+ ILK CK+A+ GKV I+D V+
Sbjct: 723 TYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVINE 902
Query: 360 GPPNLKNKETQVMSDLFFMIVNGLERDEQEWRNIIFEAGFSDYIIIPVLGVRSIIELYP* 181
+ + +++ D+ +NG ER+E+EW+ + EAGF Y I P+ G S+IE+YP*
Sbjct: 903 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1082
Query: 180 NII 172
++
Sbjct: 1083 TLL 1091
>gb|U97125.1|MSU97125 Medicago sativa isoflavone-O-methytransferase mRNA, complete cds
Length = 1231
Score = 164 bits (352), Expect = 1e-041
Identities = 68/183 (37%), Positives = 106/183 (57%)
Frame = -2 / +1
Query: 720 LMDIAVRECGDVFQGIRSLVDVXXXXXXXXXXXXXXXXAIQCTVLDLAHVVATAPACAGL 541
L+++A+R+C VF G+ S+VDV ++C V D VV L
Sbjct: 544 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 723
Query: 540 SFVAGDMFEAIPPANAVFLKWIMHDWGDSECVTILKNCKKAIPPRDAGGKVIIVDTVVGA 361
++V GDMF +IP A+AV LK+I+H+W D +C+ ILK CK+A+ GKV I+D V+
Sbjct: 724 TYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIDK 903
Query: 360 GPPNLKNKETQVMSDLFFMIVNGLERDEQEWRNIIFEAGFSDYIIIPVLGVRSIIELYP* 181
+ + +++ D+ +NG ER+E+EW+ + EAGF Y I P+ G S+IE+YP*
Sbjct: 904 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1083
Query: 180 NII 172
++
Sbjct: 1084 TLL 1092
>gb|AF000975.1|MSAF000975 Medicago sativa 7-O-methyltransferase (7-IOMT(6)) mRNA, complete cds
Length = 1284
Score = 164 bits (352), Expect = 1e-041
Identities = 68/183 (37%), Positives = 106/183 (57%)
Frame = -2 / +3
Query: 720 LMDIAVRECGDVFQGIRSLVDVXXXXXXXXXXXXXXXXAIQCTVLDLAHVVATAPACAGL 541
L+++A+R+C VF G+ S+VDV ++C V D VV L
Sbjct: 549 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 728
Query: 540 SFVAGDMFEAIPPANAVFLKWIMHDWGDSECVTILKNCKKAIPPRDAGGKVIIVDTVVGA 361
++V GDMF +IP A+AV LK+I+H+W D +C+ ILK CK+A+ GKV I+D V+
Sbjct: 729 TYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIDE 908
Query: 360 GPPNLKNKETQVMSDLFFMIVNGLERDEQEWRNIIFEAGFSDYIIIPVLGVRSIIELYP* 181
+ + +++ D+ +NG ER+E+EW+ + EAGF Y I P+ G S+IE+YP*
Sbjct: 909 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1088
Query: 180 NII 172
++
Sbjct: 1089 TLL 1097
>emb|AX050436.1| Sequence 1 from Patent WO0071736
Length = 1231
Score = 164 bits (352), Expect = 1e-041
Identities = 68/183 (37%), Positives = 106/183 (57%)
Frame = -2 / +1
Query: 720 LMDIAVRECGDVFQGIRSLVDVXXXXXXXXXXXXXXXXAIQCTVLDLAHVVATAPACAGL 541
L+++A+R+C VF G+ S+VDV ++C V D VV L
Sbjct: 544 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 723
Query: 540 SFVAGDMFEAIPPANAVFLKWIMHDWGDSECVTILKNCKKAIPPRDAGGKVIIVDTVVGA 361
++V GDMF +IP A+AV LK+I+H+W D +C+ ILK CK+A+ GKV I+D V+
Sbjct: 724 TYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIDK 903
Query: 360 GPPNLKNKETQVMSDLFFMIVNGLERDEQEWRNIIFEAGFSDYIIIPVLGVRSIIELYP* 181
+ + +++ D+ +NG ER+E+EW+ + EAGF Y I P+ G S+IE+YP*
Sbjct: 904 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1083
Query: 180 NII 172
++
Sbjct: 1084 TLL 1092
>gb|AF023481.1|AF023481 Medicago sativa o-methytransferase (iomt2003) mRNA, complete cds
Length = 1194
Score = 87.7 bits (185), Expect(2) = 4e-032
Identities = 35/94 (37%), Positives = 57/94 (60%)
Frame = -2 / +1
Query: 453 ECVTILKNCKKAIPPRDAGGKVIIVDTVVGAGPPNLKNKETQVMSDLFFMIVNGLERDEQ 274
+C+ ILK CK+A+ GKV I+D V+ + + +++ D+ +NG ER+E+
Sbjct: 787 DCLRILKKCKEAVTNDGKRGKVTIIDMVIDEKKDENQVTQIKLLMDVNMACLNGKERNEE 966
Query: 273 EWRNIIFEAGFSDYIIIPVLGVRSIIELYP*NII 172
EW+ + EAGF Y I P+ G S+IE+YP* ++
Sbjct: 967 EWKKLFIEAGFQHYKISPLTGFLSLIEIYP*TLL 1068
Score = 64.8 bits (135), Expect(2) = 4e-032
Identities = 29/80 (36%), Positives = 42/80 (52%)
Frame = -2 / +1
Query: 720 LMDIAVRECGDVFQGIRSLVDVXXXXXXXXXXXXXXXXAIQCTVLDLAHVVATAPACAGL 541
L+++A+R+C VF G+ S+VDV ++C V D VV L
Sbjct: 547 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 726
Query: 540 SFVAGDMFEAIPPANAVFLK 481
++V GDMF +IP A+AV LK
Sbjct: 727 TYVGGDMFTSIPNADAVLLK 786
>gb|M63853.1|ALFSALMCAT Medicago sativa S-adenosyl-L-methionine: caffeic acid
3-0-methyltransferase mRNA, complete cds
Length = 1317
Score = 100 bits (214), Expect = 1e-022
Identities = 40/91 (43%), Positives = 53/91 (58%)
Frame = -2 / +1
Query: 684 FQGIRSLVDVXXXXXXXXXXXXXXXXAIQCTVLDLAHVVATAPACAGLSFVAGDMFEAIP 505
F+G++SLVDV I+ DL HV+ AP+ G+ V GDMF +IP
Sbjct: 622 FEGLKSLVDVGGGTGAVINTIVSKYPTIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSIP 801
Query: 504 PANAVFLKWIMHDWGDSECVTILKNCKKAIP 412
A+AVF+KWI HDW D C+ LKNC +A+P
Sbjct: 802 KADAVFMKWICHDWSDEHCLKFLKNCYEALP 894
>emb|AX259373.1| Sequence 3 from Patent WO0173090
Length = 1098
Score = 100 bits (214), Expect = 1e-022
Identities = 40/91 (43%), Positives = 53/91 (58%)
Frame = -2 / +1
Query: 684 FQGIRSLVDVXXXXXXXXXXXXXXXXAIQCTVLDLAHVVATAPACAGLSFVAGDMFEAIP 505
F+G++SLVDV I+ DL HV+ AP+ G+ V GDMF +IP
Sbjct: 592 FEGLKSLVDVGGGTGAVINTIVSKYPTIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSIP 771
Query: 504 PANAVFLKWIMHDWGDSECVTILKNCKKAIP 412
A+AVF+KWI HDW D C+ LKNC +A+P
Sbjct: 772 KADAVFMKWICHDWSDEHCLKFLKNCYEALP 864
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,634,701
Number of Sequences: 7669
Number of extensions: 97413
Number of successful extensions: 1237
Number of sequences better than 1.0e-020: 7
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 183441060
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)