TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3067375.2.1
(1829 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF322116.2|AF322116 Medicago sativa sucrose-phosphate sy... 245 e-127
gb|AY468362.2| Medicago sativa sucrose-phosphate synthase g... 100 e-108
>gb|AF322116.2|AF322116 Medicago sativa sucrose-phosphate synthase mRNA, complete cds
Length = 3579
Score = 245 bits (530), Expect(4) = e-127
Identities = 102/195 (52%), Positives = 136/195 (69%)
Frame = -3 / +2
Query: 1203 FALDHNYRSHIEYRWGGEGLRKYLVKWASSVVERRGRTEKQVIFEDSEHSSTYCLAFRVV 1024
F D + SHIEYRWGGEGLRK LV+WA+S +++G + +Q++ + S+ YC AF+V
Sbjct: 2720 FVGDLYFHSHIEYRWGGEGLRKTLVRWAASTTDKKGESNEQIVSPVEQLSTDYCYAFKVR 2899
Query: 1023 NPNHLPPLKELQKLMRIQSLRCHALYNHGATRLSVIPMHASRSQALRYLSIRWGIELPNA 844
P PPLKEL+KLMRIQ+LRCH +Y TRL+VIP+ ASRSQALRYL +RWG EL
Sbjct: 2900 KPGMAPPLKELRKLMRIQALRCHPIYCQNGTRLNVIPVLASRSQALRYLYVRWGFELSKM 3079
Query: 843 VVIVGETGDSDYEELFGGLHKTVIVKGGFNTPANRIHTVRRYPLQDVVALDSSNIIGIEG 664
VV VGE GD+DYE L GGLHK+VI+KG + +++H R YPL DV+ +DS NI+
Sbjct: 3080 VVFVGECGDTDYEGLVGGLHKSVILKGVGSRAISQLHNNRNYPLSDVMPMDSPNIVEATE 3259
Query: 663 FSTGDIRSAMQQQLG 619
S+ A+ +++G
Sbjct: 3260 GSSSADIQALLEKVG 3304
Score = 106 bits (227), Expect(4) = 6e-060
Identities = 57/114 (50%), Positives = 64/114 (56%)
Frame = +2 / -1
Query: 833 TMTTAFGNSMPQRIDKYLRA*DRDACIGITDSLVAPWLYRA*QRSDWILINFCSSFKGGK 1012
T TT F +S P R +YLRA +RDA GIT SLV Y QR WILIN SSF+GG
Sbjct: 3090 TNTTIFDSSKPHRTYRYLRAWERDARTGITFSLVPF*QYIGWQRRAWILINLRSSFRGGA 2911
Query: 1013 *FGLTTLNARQYVEECSESSKMTCFSVLPLLSTTEEAHLTKYFLKPSPPQRYSI 1174
G TL A Q E + C + P LS E A TK FL PSPPQRYS+
Sbjct: 2910 IPGFRTLKA*Q*SVESCSTGLTICSLLSPFLSVVEAAQRTKVFLNPSPPQRYSM 2749
Score = 104 bits (221), Expect(4) = e-127
Identities = 42/65 (64%), Positives = 53/65 (81%)
Frame = -2 / +2
Query: 1825 FCKCCYFEQFGVTLIEAAMHGLPVIATKNGAPVEIYQVLENGLLVDPHDQHAIADALYKM 1646
F E FG+TLIEAA +GLP++ATKNG PV+I++VL+NGLLVDPHDQ +IADAL K+
Sbjct: 1853 FVNPAIIEPFGLTLIEAAAYGLPMVATKNGGPVDIHRVLDNGLLVDPHDQKSIADALLKL 2032
Query: 1645 LSEKQ 1631
+S KQ
Sbjct: 2033 VSNKQ 2047
Score = 100 bits (213), Expect(4) = e-127
Identities = 40/87 (45%), Positives = 59/87 (67%)
Frame = -3 / +2
Query: 1488 PVKCRKHISIIAVDSVKKEDLIQIIRNSVEATRTGTMSGSTGFVLSTSLTIAELQSVIVR 1309
P++ R + +IAVD L+++I+ EA GS GF+LSTS+TI+E+QS ++
Sbjct: 2450 PLRSRNRLFVIAVDCDTTSGLLEMIKVIFEAAGEERADGSVGFILSTSMTISEIQSFLIS 2629
Query: 1308 TGMLPTDFDAFICNSGSDIYYPLQSSD 1228
G+ P DFDA+ICNSGSD+YYP +S+
Sbjct: 2630 GGLSPNDFDAYICNSGSDLYYPSLNSE 2710
Score = 76.2 bits (160), Expect(4) = e-127
Identities = 27/42 (64%), Positives = 33/42 (78%)
Frame = -3 / +2
Query: 1623 SRCRENGLKNIHQFSWPEHCKNYLSRISSLGPRHPAFACKED 1498
++CR NGLKNIH FSWPEHCK YLS+I++ PRHP + ED
Sbjct: 2054 AKCRLNGLKNIHLFSWPEHCKTYLSKIATCKPRHPQWQRSED 2179
Score = 72.5 bits (152), Expect(4) = 6e-060
Identities = 36/50 (72%), Positives = 36/50 (72%)
Frame = +3 / -1
Query: 1656 RASAIAC*SWGSTRRPFSRT**ISTGAPFFVAITGKPCMAASIKVTPNCS 1805
RASAI *SWGST RP S T *ISTG PF VA GKP AASIKV PN S
Sbjct: 2022 RASAIDF*SWGSTSRPLSSTR*ISTGPPFLVATIGKP*AAASIKVRPNGS 1873
Score = 62.0 bits (129), Expect(4) = 6e-060
Identities = 34/78 (43%), Positives = 43/78 (55%)
Frame = +2 / -1
Query: 1256 SLPLLHIKASKSVGSMPVRTITDCSSAIVSEVDSTKPVEPDIVPVRVASTEFLII*IKSS 1435
SLPLL I ASKS+G P DC S IV EVDS P +P + AS LII +
Sbjct: 2682 SLPLLQI*ASKSLGLKPPEIRNDCISDIVIEVDSMNPTDPSALSSPAASNITLIISRRPE 2503
Query: 1436 FLTESTAMIDICFLHFTG 1489
+++STA+ + FL G
Sbjct: 2502 VVSQSTAITNSRFLLLNG 2449
Score = 57.4 bits (119), Expect(4) = 6e-060
Identities = 25/40 (62%), Positives = 29/40 (72%)
Frame = +2 / -1
Query: 1499 SSLHAKAGCLGPRLDILDK*FLQCSGQENWCIFFNPFSRH 1618
SSL GCLG ++ ILD+* LQCSG EN +FFNPFS H
Sbjct: 2178 SSLRCH*GCLGLQVAILDR*VLQCSGHENK*MFFNPFSLH 2059
>gb|AY468362.2| Medicago sativa sucrose-phosphate synthase gene, promoter region and
complete cds
Length = 8933
Score = 100 bits (213), Expect(7) = e-108
Identities = 40/87 (45%), Positives = 59/87 (67%)
Frame = -3 / +3
Query: 1488 PVKCRKHISIIAVDSVKKEDLIQIIRNSVEATRTGTMSGSTGFVLSTSLTIAELQSVIVR 1309
P++ R + +IAVD L+++I+ EA GS GF+LSTS+TI+E+QS ++
Sbjct: 7413 PLRSRNRLFVIAVDCDTTSGLLEMIKVIFEAAGEERADGSVGFILSTSMTISEIQSFLIS 7592
Query: 1308 TGMLPTDFDAFICNSGSDIYYPLQSSD 1228
G+ P DFDA+ICNSGSD+YYP +S+
Sbjct: 7593 GGLSPNDFDAYICNSGSDLYYPSLNSE 7673
Score = 99.6 bits (211), Expect(7) = e-108
Identities = 42/91 (46%), Positives = 59/91 (64%)
Frame = -3 / +2
Query: 891 ALRYLSIRWGIELPNAVVIVGETGDSDYEELFGGLHKTVIVKGGFNTPANRIHTVRRYPL 712
+ RYL +RWG EL VV VGE GD+DYE L GGLHK+VI+KG + +++H R YPL
Sbjct: 8423 SFRYLYVRWGFELSKMVVFVGECGDTDYEGLVGGLHKSVILKGVGSRAISQLHNNRNYPL 8602
Query: 711 QDVVALDSSNIIGIEGFSTGDIRSAMQQQLG 619
DV+ +DS NI+ S+ A+ +++G
Sbjct: 8603 SDVMPMDSPNIVEATEGSSSADIQALLEKVG 8695
Score = 81.7 bits (172), Expect(7) = e-108
Identities = 30/62 (48%), Positives = 43/62 (69%)
Frame = -3 / +3
Query: 1203 FALDHNYRSHIEYRWGGEGLRKYLVKWASSVVERRGRTEKQVIFEDSEHSSTYCLAFRVV 1024
F D + SHIEYRWGGEGLRK LV+WA+S +++G + +Q++ + S+ YC AF+V
Sbjct: 7683 FVGDLYFHSHIEYRWGGEGLRKTLVRWAASTTDKKGESNEQIVSPVEQLSTDYCYAFKVR 7862
Query: 1023 NP 1018
P
Sbjct: 7863 KP 7868
Score = 77.6 bits (163), Expect(7) = e-108
Identities = 32/43 (74%), Positives = 37/43 (86%)
Frame = -3 / +1
Query: 1008 PPLKELQKLMRIQSLRCHALYNHGATRLSVIPMHASRSQALRY 880
PPLKEL+KLMRIQ+LRCH +Y TRL+VIP+ ASRSQALRY
Sbjct: 8026 PPLKELRKLMRIQALRCHPIYCQNGTRLNVIPVLASRSQALRY 8154
Score = 76.2 bits (160), Expect(7) = e-108
Identities = 27/42 (64%), Positives = 33/42 (78%)
Frame = -3 / +3
Query: 1623 SRCRENGLKNIHQFSWPEHCKNYLSRISSLGPRHPAFACKED 1498
++CR NGLKNIH FSWPEHCK YLS+I++ PRHP + ED
Sbjct: 7017 AKCRLNGLKNIHLFSWPEHCKTYLSKIATCKPRHPQWQRSED 7142
Score = 62.0 bits (129), Expect(7) = 2e-045
Identities = 34/78 (43%), Positives = 43/78 (55%)
Frame = +2 / -2
Query: 1256 SLPLLHIKASKSVGSMPVRTITDCSSAIVSEVDSTKPVEPDIVPVRVASTEFLII*IKSS 1435
SLPLL I ASKS+G P DC S IV EVDS P +P + AS LII +
Sbjct: 7645 SLPLLQI*ASKSLGLKPPEIRNDCISDIVIEVDSMNPTDPSALSSPAASNITLIISRRPE 7466
Query: 1436 FLTESTAMIDICFLHFTG 1489
+++STA+ + FL G
Sbjct: 7465 VVSQSTAITNSRFLLLNG 7412
Score = 57.4 bits (119), Expect(7) = 2e-045
Identities = 25/40 (62%), Positives = 29/40 (72%)
Frame = +2 / -2
Query: 1499 SSLHAKAGCLGPRLDILDK*FLQCSGQENWCIFFNPFSRH 1618
SSL GCLG ++ ILD+* LQCSG EN +FFNPFS H
Sbjct: 7141 SSLRCH*GCLGLQVAILDR*VLQCSGHENK*MFFNPFSLH 7022
Score = 55.6 bits (115), Expect(7) = e-108
Identities = 22/31 (70%), Positives = 27/31 (87%)
Frame = -2 / +3
Query: 1723 IYQVLENGLLVDPHDQHAIADALYKMLSEKQ 1631
+ QVL+NGLLVDPHDQ +IADAL K++S KQ
Sbjct: 6918 LVQVLDNGLLVDPHDQKSIADALLKLVSNKQ 7010
Score = 50.1 bits (103), Expect(7) = 2e-045
Identities = 26/43 (60%), Positives = 28/43 (65%)
Frame = +2 / -1
Query: 881 YLRA*DRDACIGITDSLVAPWLYRA*QRSDWILINFCSSFKGG 1009
YLRA +RDA GIT SLV Y QR WILIN SSF+GG
Sbjct: 8153 YLRAWERDARTGITFSLVPF*QYIGWQRRAWILINLRSSFRGG 8025
Score = 49.6 bits (102), Expect(7) = 2e-045
Identities = 24/52 (46%), Positives = 27/52 (51%)
Frame = +2 / -2
Query: 1019 GLTTLNARQYVEECSESSKMTCFSVLPLLSTTEEAHLTKYFLKPSPPQRYSI 1174
G TL A Q E + C + P LS E A TK FL PSPPQRYS+
Sbjct: 7867 GFRTLKA*Q*SVESCSTGLTICSLLSPFLSVVEAAQRTKVFLNPSPPQRYSM 7712
Score = 40.5 bits (82), Expect(7) = e-108
Identities = 14/23 (60%), Positives = 21/23 (91%)
Frame = -2 / +3
Query: 1780 EAAMHGLPVIATKNGAPVEIYQV 1712
+AA +GLP++ATKNG PV+I++V
Sbjct: 6774 QAAAYGLPMVATKNGGPVDIHRV 6842
Score = 34.1 bits (68), Expect(7) = 2e-045
Identities = 19/66 (28%), Positives = 32/66 (48%)
Frame = +1 / -2
Query: 676 NDI*AIKSNNIL*RVPSDRMDPIRRSVESTLNDDCLVETSKQFLVIRITSFTNNDHSIWQ 855
+D+ A+ +NI R+ S M+ +L V + Q VI +T+F+N H + Q
Sbjct: 8638 DDVRAVHWHNIRERIVSVIMELTDCPASDSLQYHTFV*AANQSFVIGVTTFSNKHHHL*Q 8459
Query: 856 LYAPAN 873
P+N
Sbjct: 8458 FKTPSN 8441
Score = 33.6 bits (67), Expect(7) = 2e-045
Identities = 15/20 (75%), Positives = 15/20 (75%)
Frame = +3 / -2
Query: 1656 RASAIAC*SWGSTRRPFSRT 1715
RASAI *SWGST RP S T
Sbjct: 6985 RASAIDF*SWGSTSRPLSST 6926
Score = 30.9 bits (61), Expect(7) = 2e-045
Identities = 15/24 (62%), Positives = 16/24 (66%)
Frame = +3 / -2
Query: 1707 SRT**ISTGAPFFVAITGKPCMAA 1778
+ T *ISTG PF VA GKP AA
Sbjct: 6847 NHTR*ISTGPPFLVATIGKP*AAA 6776
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 17,554,530
Number of Sequences: 7669
Number of extensions: 275005
Number of successful extensions: 1512
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 514789580
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)