TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3042337.2.1
(945 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase ... 66 1e-037
gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase ... 66 1e-036
gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 67 6e-035
gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 65 3e-034
gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible c... 97 2e-029
gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular tr... 123 4e-029
gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular tr... 53 2e-025
gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase ... 63 9e-023
>gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase 3 (ifs3) mRNA, partial cds
Length = 1501
Score = 65.7 bits (137), Expect(4) = 1e-037
Identities = 29/83 (34%), Positives = 45/83 (54%)
Frame = -2 / +3
Query: 914 TDTMIMALCSSMFAGGSXXXXXXXEWAMSLLLSHPDVLEKAQAEIDASAGHSRLLGADDL 735
T I L F+ G+ EWA++ L+++P VLEKA+ E+ + G RL+ D
Sbjct: 828 TKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEVYSVVGKDRLVDEVDT 1007
Query: 734 PRLGYLHCIVSETLRLYPVVPTL 666
L Y+ IV ET R++P +P +
Sbjct: 1008 QNLPYIRAIVKETFRMHPPLPVV 1076
Score = 63.4 bits (132), Expect(4) = 1e-037
Identities = 22/55 (40%), Positives = 35/55 (63%)
Frame = -2 / +3
Query: 668 LVPHESTADCTVGGHRVPSGTMLLVNVYAIHRDPAIWADPAAFRPERFEDGRADG 504
+V + T +C + G+ +P G ++L NV+ + RDP W P+ FRPERF + A+G
Sbjct: 1071 VVKRKCTEECEINGYVIPEGALILFNVWQVGRDPKYWDRPSEFRPERFLETGAEG 1235
Score = 51.5 bits (106), Expect(4) = 1e-037
Identities = 21/46 (45%), Positives = 27/46 (58%)
Frame = -2 / +3
Query: 521 DGRADGLFVMPFGMGRRKCPGEALALRTLGLVLGTLIQCFDWDTVG 384
D R ++PFG GRR CPG LA + +L +LIQCFD +G
Sbjct: 1251 DLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQVLG 1388
Score = 26.7 bits (52), Expect(4) = 1e-037
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -2 / +3
Query: 386 GGAEVDMAEGGGITLPRAVPLEAI 315
G A+V M E G+T+PRA L +
Sbjct: 1413 GDAKVSMEERAGLTVPRAHSLVCV 1484
>gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase 2 (ifs2) mRNA, partial cds
Length = 1501
Score = 65.7 bits (137), Expect(4) = 1e-036
Identities = 29/83 (34%), Positives = 45/83 (54%)
Frame = -2 / +3
Query: 914 TDTMIMALCSSMFAGGSXXXXXXXEWAMSLLLSHPDVLEKAQAEIDASAGHSRLLGADDL 735
T I L F+ G+ EWA++ L+++P VLEKA+ E+ + G RL+ D
Sbjct: 828 TKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEVYSVVGKDRLVDEVDT 1007
Query: 734 PRLGYLHCIVSETLRLYPVVPTL 666
L Y+ IV ET R++P +P +
Sbjct: 1008 QNLPYIRAIVKETFRMHPPLPVV 1076
Score = 59.7 bits (124), Expect(4) = 1e-036
Identities = 21/55 (38%), Positives = 34/55 (61%)
Frame = -2 / +3
Query: 668 LVPHESTADCTVGGHRVPSGTMLLVNVYAIHRDPAIWADPAAFRPERFEDGRADG 504
+V + T +C + G+ +P G ++L NV+ + RD W P+ FRPERF + A+G
Sbjct: 1071 VVKRKCTEECEINGYVIPEGALILFNVWQVGRDSKYWDRPSEFRPERFLETGAEG 1235
Score = 51.5 bits (106), Expect(4) = 1e-036
Identities = 21/46 (45%), Positives = 27/46 (58%)
Frame = -2 / +3
Query: 521 DGRADGLFVMPFGMGRRKCPGEALALRTLGLVLGTLIQCFDWDTVG 384
D R ++PFG GRR CPG LA + +L +LIQCFD +G
Sbjct: 1251 DLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQVLG 1388
Score = 26.7 bits (52), Expect(4) = 1e-036
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -2 / +3
Query: 386 GGAEVDMAEGGGITLPRAVPLEAI 315
G A+V M E G+T+PRA L +
Sbjct: 1413 GDAKVSMEERAGLTVPRAHSLVCV 1484
>gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-K10) mRNA, complete cds
Length = 1885
Score = 67.0 bits (140), Expect(4) = 6e-035
Identities = 31/84 (36%), Positives = 45/84 (53%)
Frame = -2 / +2
Query: 914 TDTMIMALCSSMFAGGSXXXXXXXEWAMSLLLSHPDVLEKAQAEIDASAGHSRLLGADDL 735
T I A+ + GG+ EWAM+ L+ P+ L+K Q E+ G SR + D
Sbjct: 983 TKDNIKAIIMDVMFGGTETVASAIEWAMAELMKSPEDLKKVQQELAEVVGLSRQVEEPDF 1162
Query: 734 PRLGYLHCIVSETLRLYPVVPTLV 663
+L YL C + ETLRL+P +P L+
Sbjct: 1163 EKLTYLKCALKETLRLHPPIPLLL 1234
Score = 53.3 bits (110), Expect(4) = 6e-035
Identities = 18/48 (37%), Positives = 30/48 (62%)
Frame = -2 / +2
Query: 668 LVPHESTADCTVGGHRVPSGTMLLVNVYAIHRDPAIWADPAAFRPERF 525
L+ HE+ + TV G+ +P +++N +AI RD W +P +F+P RF
Sbjct: 1226 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWEEPESFKPSRF 1369
Score = 51.9 bits (107), Expect(4) = 6e-035
Identities = 21/57 (36%), Positives = 27/57 (47%)
Frame = -2 / +2
Query: 551 PAAFRPERFEDGRADGLFVMPFGMGRRKCPGEALALRTLGLVLGTLIQCFDWDTVGG 381
P+ F D + +PFG GRR CPG L L L L + L+ CF W+ G
Sbjct: 1358 PSRFLKPGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDG 1528
Score = 25.4 bits (49), Expect(4) = 6e-035
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -2 / +2
Query: 395 DTVGGAEVDMAEGGGITLPRAVPLEAI 315
D + +E+DM++ G+T PRA L AI
Sbjct: 1523 DGMKPSEMDMSDVFGLTAPRASRLIAI 1603
>gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-K1) mRNA, complete cds
Length = 1904
Score = 65.2 bits (136), Expect(4) = 3e-034
Identities = 30/84 (35%), Positives = 44/84 (52%)
Frame = -2 / +1
Query: 914 TDTMIMALCSSMFAGGSXXXXXXXEWAMSLLLSHPDVLEKAQAEIDASAGHSRLLGADDL 735
T I A+ + GG+ EW M+ L+ P+ L+K Q E+ G SR + D
Sbjct: 997 TKDNIKAIIMDVMFGGTETVASAIEWVMAELMKSPEDLKKVQQELAEVVGLSRQVEEPDF 1176
Query: 734 PRLGYLHCIVSETLRLYPVVPTLV 663
+L YL C + ETLRL+P +P L+
Sbjct: 1177 EKLTYLKCALKETLRLHPPIPLLL 1248
Score = 52.8 bits (109), Expect(4) = 3e-034
Identities = 18/48 (37%), Positives = 30/48 (62%)
Frame = -2 / +1
Query: 668 LVPHESTADCTVGGHRVPSGTMLLVNVYAIHRDPAIWADPAAFRPERF 525
L+ HE+ + TV G+ +P +++N +AI RD W +P +F+P RF
Sbjct: 1240 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRF 1383
Score = 51.9 bits (107), Expect(4) = 3e-034
Identities = 21/57 (36%), Positives = 27/57 (47%)
Frame = -2 / +1
Query: 551 PAAFRPERFEDGRADGLFVMPFGMGRRKCPGEALALRTLGLVLGTLIQCFDWDTVGG 381
P+ F D + +PFG GRR CPG L L L L + L+ CF W+ G
Sbjct: 1372 PSRFLKPGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDG 1542
Score = 25.4 bits (49), Expect(4) = 3e-034
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -2 / +1
Query: 395 DTVGGAEVDMAEGGGITLPRAVPLEAI 315
D + +E+DM++ G+T PRA L AI
Sbjct: 1537 DGMKPSEMDMSDVFGLTAPRASRLIAI 1617
>gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible cinnamic acid 4-hydroxylase mRNA,
complete cds
Length = 1744
Score = 96.8 bits (205), Expect(2) = 2e-029
Identities = 39/105 (37%), Positives = 60/105 (57%)
Frame = -2 / +3
Query: 839 WAMSLLLSHPDVLEKAQAEIDASAGHSRLLGADDLPRLGYLHCIVSETLRLYPVVPTLVP 660
W ++ L++H D+ K + E+D G + DL +L YL ++ ETLRL +P LVP
Sbjct: 981 WGIAELVNHQDIQNKVREEMDRVLGPGHQVTEPDLHKLPYLQAVIKETLRLRMAIPLLVP 1160
Query: 659 HESTADCTVGGHRVPSGTMLLVNVYAIHRDPAIWADPAAFRPERF 525
H + D + G +P+ + +LVN + + +PA W P FRPERF
Sbjct: 1161 HMNLHDPKLNGFDIPAESKILVNAWWLPNNPAHWKKPEEFRPERF 1295
Score = 46.9 bits (96), Expect(2) = 2e-029
Identities = 17/29 (58%), Positives = 22/29 (75%)
Frame = -2 / +3
Query: 494 MPFGMGRRKCPGEALALRTLGLVLGTLIQ 408
+PFG+GRR CPG LAL LG+ +G L+Q
Sbjct: 1344 LPFGVGRRSCPGIILALPILGITIGRLVQ 1430
>gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 506
Score = 123 bits (263), Expect = 4e-029
Identities = 49/125 (39%), Positives = 74/125 (59%)
Frame = -2 / +2
Query: 881 MFAGGSXXXXXXXEWAMSLLLSHPDVLEKAQAEIDASAGHSRLLGADDLPRLGYLHCIVS 702
MF GG+ WAMS ++ +P V+EKAQ E+ + +L +L YL ++
Sbjct: 92 MFVGGTESSSEVVLWAMSEMVKNPMVMEKAQVEVRRVFDKKGYVDETELHQLIYLKSVIK 271
Query: 701 ETLRLYPVVPTLVPHESTADCTVGGHRVPSGTMLLVNVYAIHRDPAIWADPAAFRPERFE 522
ET+RL+P +P L+P ES C + G+ +P+ T ++VN +AI RDP W D +F+PERF
Sbjct: 272 ETMRLHPSLPLLIPRESIKPCQINGYDIPAKTRVIVNAWAIGRDPRYWVDAESFKPERFL 451
Query: 521 DGRAD 507
+ R D
Sbjct: 452 NSRFD 466
>gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 52.8 bits (109), Expect(4) = 2e-025
Identities = 18/48 (37%), Positives = 30/48 (62%)
Frame = -2 / +2
Query: 668 LVPHESTADCTVGGHRVPSGTMLLVNVYAIHRDPAIWADPAAFRPERF 525
L+ HE+ + TV G+ +P +++N +AI RD W +P +F+P RF
Sbjct: 86 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRF 229
Score = 51.0 bits (105), Expect(4) = 2e-025
Identities = 21/57 (36%), Positives = 27/57 (47%)
Frame = -2 / +2
Query: 551 PAAFRPERFEDGRADGLFVMPFGMGRRKCPGEALALRTLGLVLGTLIQCFDWDTVGG 381
P+ F D + +PFG GRR CPG L L L L + L+ CF W+ G
Sbjct: 218 PSRFLKPGEPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDG 388
Score = 35.9 bits (72), Expect(4) = 2e-025
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = -2 / +2
Query: 740 DLPRLGYLHCIVSETLRLYPVVPTLV 663
D +L YL C + ETLRL+P +P L+
Sbjct: 17 DFEKLTYLKCALKETLRLHPPIPLLL 94
Score = 25.4 bits (49), Expect(4) = 2e-025
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -2 / +2
Query: 395 DTVGGAEVDMAEGGGITLPRAVPLEAI 315
D + +E+DM++ G+T PRA L AI
Sbjct: 383 DGMKPSEMDMSDVFGLTAPRASRLIAI 463
>gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase 1 (ifs1) mRNA, partial cds
Length = 1501
Score = 62.9 bits (131), Expect(3) = 9e-023
Identities = 22/55 (40%), Positives = 35/55 (63%)
Frame = -2 / +3
Query: 668 LVPHESTADCTVGGHRVPSGTMLLVNVYAIHRDPAIWADPAAFRPERFEDGRADG 504
+V + T +C + G+ +P G ++L NV+ + RDP W P+ FRPERF + A+G
Sbjct: 1071 VVKRKCTEECEINGYVIPEGALVLFNVWQVGRDPKYWDRPSEFRPERFLETGAEG 1235
Score = 51.5 bits (106), Expect(3) = 9e-023
Identities = 21/46 (45%), Positives = 27/46 (58%)
Frame = -2 / +3
Query: 521 DGRADGLFVMPFGMGRRKCPGEALALRTLGLVLGTLIQCFDWDTVG 384
D R ++PFG GRR CPG LA + +L +LIQCFD +G
Sbjct: 1251 DLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQVLG 1388
Score = 24.4 bits (47), Expect(3) = 9e-023
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = -2 / +3
Query: 380 AEVDMAEGGGITLPRAVPLEAI 315
A+V M E G+T+PRA L +
Sbjct: 1419 AKVSMEERAGLTVPRAHSLVCV 1484
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,760,282
Number of Sequences: 7669
Number of extensions: 75016
Number of successful extensions: 728
Number of sequences better than 1.0e-020: 8
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 248995071
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)