TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3012873.2.2
(1520 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512887.1|CO512887 s13dSG20H0700060_121700 Glandular tr... 79 3e-039
gb|CO512956.1|CO512956 s13dSG86G1100084_121838 Glandular tr... 77 5e-038
emb|X80222.1|MSDFR1 M.sativa mRNA for dihydroflavonol-4-red... 44 3e-023
gb|U28213.1|MSU28213 Medicago sativa vestitone reductase mR... 51 3e-022
>gb|CO512887.1|CO512887 s13dSG20H0700060_121700 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 602
Score = 79.4 bits (167), Expect(3) = 3e-039
Identities = 30/39 (76%), Positives = 35/39 (89%)
Frame = +3 / +1
Query: 294 GQTVCVTGAAGYIASWLVKLLLEKGYTVKGTVRNPDDPK 410
G VCVTGA+GYIASWLV+LLL +GYTVK TVR+P+DPK
Sbjct: 61 GNVVCVTGASGYIASWLVRLLLNRGYTVKATVRDPNDPK 177
Score = 62.9 bits (131), Expect(3) = 3e-039
Identities = 29/51 (56%), Positives = 32/51 (62%)
Frame = +3 / +1
Query: 399 DDPKNAHLKALDGAAERLILCKADLLDYDAICRAVQGCQGVFHTASPVTDD 551
D K HL L+GA ERL L KA+LL+ A VQGC GVFHTASP D
Sbjct: 169 DPKKGDHLVKLEGAKERLQLFKANLLEEGAFDSVVQGCHGVFHTASPFYHD 321
Score = 53.3 bits (110), Expect(3) = 3e-039
Identities = 22/55 (40%), Positives = 31/55 (56%)
Frame = +3 / +1
Query: 627 RRVVFTSSIGAVTMDPKRGPDVVVDESCWSDLEFCEKTRNWYCYGKAVAEHAAWE 791
R V+ +S R PDVVVDE+ ++D + C K+ WY K +AE AAW+
Sbjct: 412 RLVLTSSIAAVAYNGKPRTPDVVVDETWFTDADLCAKSNLWYVVSKTLAEEAAWK 576
>gb|CO512956.1|CO512956 s13dSG86G1100084_121838 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 584
Score = 76.7 bits (161), Expect(3) = 5e-038
Identities = 30/39 (76%), Positives = 34/39 (87%)
Frame = +3 / +1
Query: 294 GQTVCVTGAAGYIASWLVKLLLEKGYTVKGTVRNPDDPK 410
G VCVTGA+GYIASWLV LLL +GYTVK TVR+P+DPK
Sbjct: 61 GNVVCVTGASGYIASWLV*LLLNRGYTVKATVRDPNDPK 177
Score = 61.6 bits (128), Expect(3) = 5e-038
Identities = 29/51 (56%), Positives = 32/51 (62%)
Frame = +3 / +1
Query: 399 DDPKNAHLKALDGAAERLILCKADLLDYDAICRAVQGCQGVFHTASPVTDD 551
D K HL L+GA ERL L KA+LL+ A VQGC GVFHTASP D
Sbjct: 169 DPKKVDHLVKLEGAKERLQLFKANLLEEGAFDSVVQGCHGVFHTASPFYHD 321
Score = 53.3 bits (110), Expect(3) = 5e-038
Identities = 22/55 (40%), Positives = 31/55 (56%)
Frame = +3 / +1
Query: 627 RRVVFTSSIGAVTMDPKRGPDVVVDESCWSDLEFCEKTRNWYCYGKAVAEHAAWE 791
R V+ +S R PDVVVDE+ ++D + C K+ WY K +AE AAW+
Sbjct: 412 RLVLTSSIAAVAYNGKPRTPDVVVDETWFTDADLCAKSNLWYVVSKTLAEEAAWK 576
>emb|X80222.1|MSDFR1 M.sativa mRNA for dihydroflavonol-4-reductase
Length = 650
Score = 43.7 bits (89), Expect(4) = 3e-023
Identities = 19/45 (42%), Positives = 28/45 (62%)
Frame = +3 / +1
Query: 408 KNAHLKALDGAAERLILCKADLLDYDAICRAVQGCQGVFHTASPV 542
K + L L GA +L + KADL + + A++GC GVFH A+P+
Sbjct: 79 KVSPLLELPGAKSKLSIWKADLGEEGSFDEAIKGCTGVFHVATPM 213
Score = 42.3 bits (86), Expect(4) = 3e-023
Identities = 15/47 (31%), Positives = 29/47 (61%)
Frame = +3 / +1
Query: 546 DDPEQMVEPAVRGTEYVINAAAEAGTVRRVVFTSSIGAVTMDPKRGP 686
D +M++P ++G ++ A +A TVRR+++TSS G + + + P
Sbjct: 229 DPENEMIKPTIKGVLDIMKACLKAKTVRRLIYTSSAGTLNVTEDQKP 369
Score = 37.3 bits (75), Expect(4) = 3e-023
Identities = 14/25 (56%), Positives = 19/25 (76%)
Frame = +3 / +1
Query: 336 SWLVKLLLEKGYTVKGTVRNPDDPK 410
SWLV L+E GY V+ TVR+P++ K
Sbjct: 4 SWLVMRLMEPGYMVRATVRDPENLK 78
Score = 35.0 bits (70), Expect(4) = 3e-023
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +3 / +1
Query: 699 DESCWSDLEFCEKTR 743
DESCWSD+EFC + +
Sbjct: 376 DESCWSDVEFCRRVK 420
>gb|U28213.1|MSU28213 Medicago sativa vestitone reductase mRNA, complete cds
Length = 1237
Score = 51.0 bits (105), Expect(4) = 3e-022
Identities = 20/41 (48%), Positives = 28/41 (68%)
Frame = +3 / +2
Query: 420 LKALDGAAERLILCKADLLDYDAICRAVQGCQGVFHTASPV 542
L L GA+E+L ADL + D+ A++GC G+FHTASP+
Sbjct: 197 LTNLPGASEKLHFFNADLSNPDSFAAAIEGCVGIFHTASPI 319
Score = 51.0 bits (105), Expect(4) = 3e-022
Identities = 18/36 (50%), Positives = 25/36 (69%)
Frame = +3 / +2
Query: 303 VCVTGAAGYIASWLVKLLLEKGYTVKGTVRNPDDPK 410
VCVTG G++ SW++K LLE GY+V T+R + K
Sbjct: 74 VCVTGGTGFLGSWIIKSLLENGYSVNTTIRADPERK 181
Score = 40.0 bits (81), Expect(4) = 3e-022
Identities = 16/48 (33%), Positives = 30/48 (62%)
Frame = +3 / +2
Query: 945 VDVRDVADAHLRVFESPRASGRHLCAERVLHREDVVRILAKLFPEYPV 1088
V V DVA AH+ + E+ GR+ C+ ++ E++ ++L+ +PEY +
Sbjct: 737 VHVDDVARAHIYLLENSVPGGRYNCSPFIVPIEEMSQLLSAKYPEYQI 880
Score = 22.1 bits (42), Expect(4) = 3e-022
Identities = 7/17 (41%), Positives = 11/17 (64%)
Frame = +3 / +2
Query: 693 VVDESCWSDLEFCEKTR 743
V+DES WSD++ +
Sbjct: 476 VLDESDWSDVDLLRSVK 526
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,377,743
Number of Sequences: 7669
Number of extensions: 95479
Number of successful extensions: 833
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 420942984
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)