TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2949673.2.1
(636 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF083333.1|AF083333 Medicago sativa cinnamyl-alcohol deh... 181 7e-047
gb|L46856.1|ALFCAD1A Medicago sativa cinnamyl-alcohol dehyd... 181 7e-047
gb|AF083332.1|AF083332 Medicago sativa cinnamyl-alcohol deh... 78 4e-033
emb|Z19573.1|MSCIALDHA M.sativa encoding cinnamyl alcohol d... 78 4e-033
gb|L46857.1|ALFCAD1B Medicago sativa cinnamyl-alcohol dehyd... 78 4e-033
>gb|AF083333.1|AF083333 Medicago sativa cinnamyl-alcohol dehydrogenase (MsaCad1) mRNA,
complete cds
Length = 1390
Score = 181 bits (390), Expect = 7e-047
Identities = 77/145 (53%), Positives = 103/145 (71%)
Frame = -2 / +3
Query: 635 VISTSLGKRDEALGRLGADAFLVSRDPEQMRAAAGTLDGVIDTVSADHPVVPLLDLLKPM 456
VISTS K EA+ +LGAD+FLVS+DPE+M+AA GT+D +IDT+SA H ++PLL LLK
Sbjct: 732 VISTSPNKETEAIDKLGADSFLVSKDPEKMKAAMGTMDYIIDTISAAHSLMPLLGLLKLN 911
Query: 455 GQMVVVGLPTKPLQVPAFSLXXXXXXXXXXXXXXXGECQAMLDFAGEHGITADVEVVGMD 276
G++V VGLP+KPL++ F L E Q MLDF G+H ITAD+E++ M
Sbjct: 912 GKLVTVGLPSKPLELSVFPLVAGRKLIGGSNIGGMKETQEMLDFCGKHNITADIELIKMH 1091
Query: 275 YVNTAIQRLERNDVRYRFVVDVAGS 201
+NTA++RL + DV+YRFV+DVA S
Sbjct: 1092 EINTAMERLHKADVKYRFVIDVANS 1166
>gb|L46856.1|ALFCAD1A Medicago sativa cinnamyl-alcohol dehydrogenase (cad1) mRNA, complete
cds
Length = 1390
Score = 181 bits (390), Expect = 7e-047
Identities = 77/145 (53%), Positives = 103/145 (71%)
Frame = -2 / +3
Query: 635 VISTSLGKRDEALGRLGADAFLVSRDPEQMRAAAGTLDGVIDTVSADHPVVPLLDLLKPM 456
VISTS K EA+ +LGAD+FLVS+DPE+M+AA GT+D +IDT+SA H ++PLL LLK
Sbjct: 732 VISTSPNKETEAIDKLGADSFLVSKDPEKMKAAMGTMDYIIDTISAAHSLMPLLGLLKLN 911
Query: 455 GQMVVVGLPTKPLQVPAFSLXXXXXXXXXXXXXXXGECQAMLDFAGEHGITADVEVVGMD 276
G++V VGLP+KPL++ F L E Q MLDF G+H ITAD+E++ M
Sbjct: 912 GKLVTVGLPSKPLELSVFPLVAGRKLIGGSNIGGMKETQEMLDFCGKHNITADIELIKMH 1091
Query: 275 YVNTAIQRLERNDVRYRFVVDVAGS 201
+NTA++RL + DV+YRFV+DVA S
Sbjct: 1092 EINTAMERLHKADVKYRFVIDVANS 1166
>gb|AF083332.1|AF083332 Medicago sativa cinnamyl-alcohol dehydrogenase (MsaCad2) mRNA,
complete cds
Length = 1324
Score = 78.0 bits (164), Expect(2) = 4e-033
Identities = 31/50 (62%), Positives = 40/50 (80%)
Frame = -2 / +3
Query: 347 ECQAMLDFAGEHGITADVEVVGMDYVNTAIQRLERNDVRYRFVVDVAGSK 198
E + ML+F E G+T+ +E+V MDY+N A +RLE+NDVRYRFVVDV GSK
Sbjct: 972 ETEEMLEFWKEKGLTSMIEIVTMDYINKAFERLEKNDVRYRFVVDVKGSK 1121
Score = 77.6 bits (163), Expect(2) = 4e-033
Identities = 37/74 (50%), Positives = 50/74 (67%)
Frame = -2 / +3
Query: 635 VISTSLGKRDEALGRLGADAFLVSRDPEQMRAAAGTLDGVIDTVSADHPVVPLLDLLKPM 456
VIS+S K+ EAL LGAD +LVS D M+ AA +LD +IDTV HP+ P L LLK
Sbjct: 684 VISSSDKKKKEALEDLGADNYLVSSDTVGMQEAADSLDYIIDTVPVGHPLEPYLSLLKID 863
Query: 455 GQMVVVGLPTKPLQ 414
G+++++G+ PLQ
Sbjct: 864 GKLILMGVINTPLQ 905
>emb|Z19573.1|MSCIALDHA M.sativa encoding cinnamyl alcohol dehydrogenase
Length = 1321
Score = 78.0 bits (164), Expect(2) = 4e-033
Identities = 31/50 (62%), Positives = 40/50 (80%)
Frame = -2 / +3
Query: 347 ECQAMLDFAGEHGITADVEVVGMDYVNTAIQRLERNDVRYRFVVDVAGSK 198
E + ML+F E G+T+ +E+V MDY+N A +RLE+NDVRYRFVVDV GSK
Sbjct: 996 ETEEMLEFWKEKGLTSMIEIVTMDYINKAFERLEKNDVRYRFVVDVKGSK 1145
Score = 77.6 bits (163), Expect(2) = 4e-033
Identities = 37/74 (50%), Positives = 50/74 (67%)
Frame = -2 / +3
Query: 635 VISTSLGKRDEALGRLGADAFLVSRDPEQMRAAAGTLDGVIDTVSADHPVVPLLDLLKPM 456
VIS+S K+ EAL LGAD +LVS D M+ AA +LD +IDTV HP+ P L LLK
Sbjct: 708 VISSSDKKKKEALEDLGADNYLVSSDTVGMQEAADSLDYIIDTVPVGHPLEPYLSLLKID 887
Query: 455 GQMVVVGLPTKPLQ 414
G+++++G+ PLQ
Sbjct: 888 GKLILMGVINTPLQ 929
>gb|L46857.1|ALFCAD1B Medicago sativa cinnamyl-alcohol dehydrogenase (cad1) mRNA, partial
cds
Length = 1201
Score = 78.0 bits (164), Expect(2) = 4e-033
Identities = 31/50 (62%), Positives = 40/50 (80%)
Frame = -2 / +3
Query: 347 ECQAMLDFAGEHGITADVEVVGMDYVNTAIQRLERNDVRYRFVVDVAGSK 198
E + ML+F E G+T+ +E+V MDY+N A +RLE+NDVRYRFVVDV GSK
Sbjct: 849 ETEEMLEFWKEKGLTSMIEIVTMDYINKAFERLEKNDVRYRFVVDVKGSK 998
Score = 77.6 bits (163), Expect(2) = 4e-033
Identities = 37/74 (50%), Positives = 50/74 (67%)
Frame = -2 / +3
Query: 635 VISTSLGKRDEALGRLGADAFLVSRDPEQMRAAAGTLDGVIDTVSADHPVVPLLDLLKPM 456
VIS+S K+ EAL LGAD +LVS D M+ AA +LD +IDTV HP+ P L LLK
Sbjct: 561 VISSSDKKKKEALEDLGADNYLVSSDTVGMQEAADSLDYIIDTVPVGHPLEPYLSLLKID 740
Query: 455 GQMVVVGLPTKPLQ 414
G+++++G+ PLQ
Sbjct: 741 GKLILMGVINTPLQ 782
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,170,396
Number of Sequences: 7669
Number of extensions: 49563
Number of successful extensions: 887
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 156573430
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)