TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 2945733.2.1
         (885 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AF172172.1|AF172172  Medicago sativa non-symbiotic hemogl...   218   4e-067

>gb|AF172172.1|AF172172 Medicago sativa non-symbiotic hemoglobin (MHB1) mRNA, complete cds
          Length = 483

 Score =  218 bits (471), Expect(2) = 4e-067
 Identities = 90/122 (73%), Positives = 108/122 (88%)
 Frame = +1 / +1

                                                                       
Query: 208 FGEEQEALVLKSWAVMKKDAANLGLRFFLKVFEIAPSAKQMFSFLRDSDVPLEKNPKLKT 387
           F EEQEALV+KSW  MKK++A LGL+ FLK+FEIAPSA+++FSFL+DS VPLE+N KLK 
Sbjct: 25  FTEEQEALVVKSWNAMKKNSAELGLKLFLKIFEIAPSAQKLFSFLKDSKVPLEQNTKLKP 204

                                                                       
Query: 388 HAMSVFVMTCEAAAQLRKAGKVTVRETTLKRLGATHLRYGVADGHFEVTGFALLETIKEA 567
           HAMSVF+MTCE+A QLRK+GKVTVRE++LK+LGA H +YGV D HFEVT FALLETIKEA
Sbjct: 205 HAMSVFLMTCESAVQLRKSGKVTVRESSLKKLGANHFKYGVVDEHFEVTKFALLETIKEA 384

             
Query: 568 LP 573
           +P
Sbjct: 385 VP 390




 Score =  108 bits (231), Expect(2) = 9e-028
 Identities = 55/100 (55%), Positives = 61/100 (61%)
 Frame = -2 / -2

                                                                       
Query: 539 NPVTSKCPSATPYLKCVAPSLLSVVSLTVTFPALRSCAAASQVMTKTDMACVLSLGFFSS 360
           N VTSKC S TPYLK +AP+   + SLTVT P  RS  A S VM KTDMA  LSL F S 
Sbjct: 356 NFVTSKCSSTTPYLKWLAPNFFKLDSLTVTLPDFRS*TADSHVMRKTDMA*GLSLVFCSK 177

                                                   
Query: 359 GTSESRRNENICFADGAISKTLRKKRRPRLAASFFMTAHD 240
           GT ES +NE    ADGAIS   +K   P  A  FF+  HD
Sbjct: 176 GTFESFKNEKSF*ADGAISNIFKKSFSPNSAEFFFIAFHD 57




 Score = 51.5 bits (106), Expect(2) = 4e-067
 Identities = 21/31 (67%), Positives = 23/31 (74%)
 Frame = +1 / +1

                                          
Query: 577 DMWSLEMKKAWAEAYSQLVAAIKREMKPDA* 669
           +MWS  MK AW EAY QLV AIK EMKP +*
Sbjct: 391 EMWSPAMKNAWGEAYDQLVNAIKSEMKPSS* 483




 Score = 29.5 bits (58), Expect(2) = 9e-028
 Identities = 15/27 (55%), Positives = 19/27 (70%)
 Frame = -2 / -2

                                      
Query: 659 GFISRLMAATSWL*ASAQAFFISRLHM 579
           GFIS L+A T+  *AS  AFFI+  H+
Sbjct: 473 GFISDLIALTN*S*ASPHAFFIAGDHI 393



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 5,173,698
Number of Sequences: 7669
Number of extensions: 58786
Number of successful extensions: 697
Number of sequences better than 1.0e-020: 1
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 233470440
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)