TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2943843.2.1
(2639 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|AX776636.1| Sequence 5 from Patent WO02102841 113 1e-062
emb|AJ418368.1|MSA418368 Medicago sativa mRNA for nodulatio... 113 1e-062
emb|AJ418377.1|MSA418377 Medicago sativa mRNA for nodulatio... 111 1e-060
emb|AX776635.1| Sequence 4 from Patent WO02102841 111 1e-060
gb|CO511940.1|CO511940 s13dSG05G1200100_103780 Glandular tr... 133 1e-051
gb|CO512543.1|CO512543 s13dSG08D0400030_121012 Glandular tr... 79 1e-034
gb|AY563141.1| Medicago sativa calcium/calmodulin-regulated... 69 4e-028
gb|CO512977.1|CO512977 s13dSG09B0200013_121880 Glandular tr... 66 7e-027
>emb|AX776636.1| Sequence 5 from Patent WO02102841
Length = 3339
Score = 113 bits (242), Expect(6) = 1e-062
Identities = 50/117 (42%), Positives = 70/117 (59%)
Frame = -1 / +3
Query: 1160 QSSDDFAELVSNISKLHHPNLNELVGYCMEHGQHLLVYDFHRNGSLHDLLHLSDEYSKPL 981
Q + +F ++ +S + H NL L+GYC E+ Q +LVY F NGSL D L+ K L
Sbjct: 2139 QGTXEFDNELNLLSAIQHENLVPLLGYCNEYDQQILVYPFMSNGSLLDRLYGEASKRKIL 2318
Query: 980 SWNSRIKIALGSARALEYLHEICSPSIIHKNFKSSNILLDSEFNPHLSDAGLASFIP 810
W +R+ IALG+AR L YLH S+IH++ KSSNILLD ++D G + + P
Sbjct: 2319 DWPTRLSIALGAARGLAYLHTFPGRSVIHRDVKSSNILLDQSMCAKVADFGFSKYAP 2489
Score = 74.8 bits (157), Expect(6) = 1e-062
Identities = 31/57 (54%), Positives = 40/57 (70%)
Frame = -1 / +3
Query: 782 SAGYTAPEVDMTGQYTFKSDVYSFGVVMLELLTGRRPFDSSRPRSEQSLVRWATPQL 612
+AGY PE T Q + KSDV+SFGVV+LE+++GR P + RPR E SLV WA P +
Sbjct: 2529 TAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEWSLVEWAKPYI 2699
Score = 46.4 bits (95), Expect(6) = 1e-062
Identities = 18/46 (39%), Positives = 30/46 (65%)
Frame = -1 / +3
Query: 596 LDRMVDPALKGLYPAKSLSRFADVLALCVQPEPEFRPPMSEVVQAL 459
+D +VDP +KG Y A++L R +V C++P +RP M ++V+ L
Sbjct: 2712 VDEIVDPGIKGGYHAEALWRVVEVALQCLEPYSTYRPCMVDIVREL 2849
Score = 40.9 bits (83), Expect(6) = 1e-062
Identities = 15/31 (48%), Positives = 22/31 (70%)
Frame = -1 / +3
Query: 2132 LNLAGNQFGGNLPYSISTMPNLKYLNLNHNQ 2040
L+L+ N G +PY ++ M NL+ LNL+HNQ
Sbjct: 1476 LDLSSNNLKGAIPYFVTKMTNLQILNLSHNQ 1568
Score = 39.6 bits (80), Expect(6) = 1e-062
Identities = 15/32 (46%), Positives = 21/32 (65%)
Frame = -1 / +3
Query: 1268 FDNLVGEGTFGRVYRAQFNDGKVLAIKKLDST 1173
+ L+GEG FG VYR +DG+ +A+K ST
Sbjct: 2037 YKTLIGEGGFGSVYRGTLDDGQEVAVKVRSST 2132
Score = 36.8 bits (74), Expect(6) = 1e-062
Identities = 16/35 (45%), Positives = 20/35 (57%)
Frame = -1 / +3
Query: 1997 LSELDLSFNSLTGDLPQSFTGLSSLKRMYLQNNQF 1893
L LDLS+N L G LP+S L LK +Y N +
Sbjct: 1608 LISLDLSYNDLDGRLPESIISLPHLKSLYFGCNPY 1712
>emb|AJ418368.1|MSA418368 Medicago sativa mRNA for nodulation receptor kinase (nork gene), wild
type allele DMI2
Length = 3339
Score = 113 bits (242), Expect(6) = 1e-062
Identities = 50/117 (42%), Positives = 70/117 (59%)
Frame = -1 / +3
Query: 1160 QSSDDFAELVSNISKLHHPNLNELVGYCMEHGQHLLVYDFHRNGSLHDLLHLSDEYSKPL 981
Q + +F ++ +S + H NL L+GYC E+ Q +LVY F NGSL D L+ K L
Sbjct: 2139 QGTXEFDNELNLLSAIQHENLVPLLGYCNEYDQQILVYPFMSNGSLLDRLYGEASKRKIL 2318
Query: 980 SWNSRIKIALGSARALEYLHEICSPSIIHKNFKSSNILLDSEFNPHLSDAGLASFIP 810
W +R+ IALG+AR L YLH S+IH++ KSSNILLD ++D G + + P
Sbjct: 2319 DWPTRLSIALGAARGLAYLHTFPGRSVIHRDVKSSNILLDQSMCAKVADFGFSKYAP 2489
Score = 74.8 bits (157), Expect(6) = 1e-062
Identities = 31/57 (54%), Positives = 40/57 (70%)
Frame = -1 / +3
Query: 782 SAGYTAPEVDMTGQYTFKSDVYSFGVVMLELLTGRRPFDSSRPRSEQSLVRWATPQL 612
+AGY PE T Q + KSDV+SFGVV+LE+++GR P + RPR E SLV WA P +
Sbjct: 2529 TAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEWSLVEWAKPYI 2699
Score = 46.4 bits (95), Expect(6) = 1e-062
Identities = 18/46 (39%), Positives = 30/46 (65%)
Frame = -1 / +3
Query: 596 LDRMVDPALKGLYPAKSLSRFADVLALCVQPEPEFRPPMSEVVQAL 459
+D +VDP +KG Y A++L R +V C++P +RP M ++V+ L
Sbjct: 2712 VDEIVDPGIKGGYHAEALWRVVEVALQCLEPYSTYRPCMVDIVREL 2849
Score = 40.9 bits (83), Expect(6) = 1e-062
Identities = 15/31 (48%), Positives = 22/31 (70%)
Frame = -1 / +3
Query: 2132 LNLAGNQFGGNLPYSISTMPNLKYLNLNHNQ 2040
L+L+ N G +PY ++ M NL+ LNL+HNQ
Sbjct: 1476 LDLSSNNLKGAIPYFVTKMTNLQILNLSHNQ 1568
Score = 39.6 bits (80), Expect(6) = 1e-062
Identities = 15/32 (46%), Positives = 21/32 (65%)
Frame = -1 / +3
Query: 1268 FDNLVGEGTFGRVYRAQFNDGKVLAIKKLDST 1173
+ L+GEG FG VYR +DG+ +A+K ST
Sbjct: 2037 YKTLIGEGGFGSVYRGTLDDGQEVAVKVRSST 2132
Score = 36.8 bits (74), Expect(6) = 1e-062
Identities = 16/35 (45%), Positives = 20/35 (57%)
Frame = -1 / +3
Query: 1997 LSELDLSFNSLTGDLPQSFTGLSSLKRMYLQNNQF 1893
L LDLS+N L G LP+S L LK +Y N +
Sbjct: 1608 LISLDLSYNDLDGRLPESIISLPHLKSLYFGCNPY 1712
>emb|AJ418377.1|MSA418377 Medicago sativa mRNA for nodulation receptor kinase (nork gene),
mutant allele nn1
Length = 3337
Score = 111 bits (236), Expect(6) = 1e-060
Identities = 49/117 (41%), Positives = 69/117 (58%)
Frame = -1 / +1
Query: 1160 QSSDDFAELVSNISKLHHPNLNELVGYCMEHGQHLLVYDFHRNGSLHDLLHLSDEYSKPL 981
Q + +F ++ +S + H NL L+GYC E+ Q +LVY F NGSL D L+ K L
Sbjct: 2137 QGTKEFDNELNLLSAIQHENLVPLLGYCNEYDQQILVYPFMSNGSLLDRLYGEASKRKIL 2316
Query: 980 SWNSRIKIALGSARALEYLHEICSPSIIHKNFKSSNILLDSEFNPHLSDAGLASFIP 810
W +R+ IALG+ R L LH PS+IH++ KSSNILLD ++D G + + P
Sbjct: 2317 DWPTRLSIALGAPRGLA*LHTFPGPSVIHRDLKSSNILLDQSMCAKVADFGFSKYAP 2487
Score = 74.8 bits (157), Expect(6) = 1e-060
Identities = 31/57 (54%), Positives = 40/57 (70%)
Frame = -1 / +1
Query: 782 SAGYTAPEVDMTGQYTFKSDVYSFGVVMLELLTGRRPFDSSRPRSEQSLVRWATPQL 612
+AGY PE T Q + KSDV+SFGVV+LE+++GR P + RPR E SLV WA P +
Sbjct: 2527 TAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEWSLVEWAKPYI 2697
Score = 46.4 bits (95), Expect(6) = 1e-060
Identities = 18/46 (39%), Positives = 30/46 (65%)
Frame = -1 / +1
Query: 596 LDRMVDPALKGLYPAKSLSRFADVLALCVQPEPEFRPPMSEVVQAL 459
+D +VDP +KG Y A++L R +V C++P +RP M ++V+ L
Sbjct: 2710 VDEIVDPGIKGGYHAEALWRVVEVALQCLEPYSTYRPCMVDIVREL 2847
Score = 40.9 bits (83), Expect(6) = 1e-060
Identities = 15/31 (48%), Positives = 22/31 (70%)
Frame = -1 / +1
Query: 2132 LNLAGNQFGGNLPYSISTMPNLKYLNLNHNQ 2040
L+L+ N G +PY ++ M NL+ LNL+HNQ
Sbjct: 1474 LDLSSNNLKGAIPYFVTKMTNLQILNLSHNQ 1566
Score = 36.8 bits (74), Expect(6) = 1e-060
Identities = 16/35 (45%), Positives = 20/35 (57%)
Frame = -1 / +1
Query: 1997 LSELDLSFNSLTGDLPQSFTGLSSLKRMYLQNNQF 1893
L LDLS+N L G LP+S L LK +Y N +
Sbjct: 1606 LISLDLSYNDLDGRLPESIISLPHLKSLYFGCNPY 1710
Score = 35.4 bits (71), Expect(6) = 1e-060
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -1 / +1
Query: 1268 FDNLVGEGTFGRVYRAQFNDGKVLAIKKLDST 1173
+ L+ EG FG VYR +DG+ +A+K ST
Sbjct: 2035 YKTLICEGGFGSVYRGTLDDGQEVAVKVRSST 2130
>emb|AX776635.1| Sequence 4 from Patent WO02102841
Length = 3378
Score = 111 bits (236), Expect(6) = 1e-060
Identities = 49/117 (41%), Positives = 69/117 (58%)
Frame = -1 / +1
Query: 1160 QSSDDFAELVSNISKLHHPNLNELVGYCMEHGQHLLVYDFHRNGSLHDLLHLSDEYSKPL 981
Q + +F ++ +S + H NL L+GYC E+ Q +LVY F NGSL D L+ K L
Sbjct: 2158 QGTKEFDNELNLLSAIQHENLVPLLGYCNEYDQQILVYPFMSNGSLLDRLYGEASKRKIL 2337
Query: 980 SWNSRIKIALGSARALEYLHEICSPSIIHKNFKSSNILLDSEFNPHLSDAGLASFIP 810
W +R+ IALG+ R L LH PS+IH++ KSSNILLD ++D G + + P
Sbjct: 2338 DWPTRLSIALGAPRGLA*LHTFPGPSVIHRDLKSSNILLDQSMCAKVADFGFSKYAP 2508
Score = 74.8 bits (157), Expect(6) = 1e-060
Identities = 31/57 (54%), Positives = 40/57 (70%)
Frame = -1 / +1
Query: 782 SAGYTAPEVDMTGQYTFKSDVYSFGVVMLELLTGRRPFDSSRPRSEQSLVRWATPQL 612
+AGY PE T Q + KSDV+SFGVV+LE+++GR P + RPR E SLV WA P +
Sbjct: 2548 TAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEWSLVEWAKPYI 2718
Score = 46.4 bits (95), Expect(6) = 1e-060
Identities = 18/46 (39%), Positives = 30/46 (65%)
Frame = -1 / +1
Query: 596 LDRMVDPALKGLYPAKSLSRFADVLALCVQPEPEFRPPMSEVVQAL 459
+D +VDP +KG Y A++L R +V C++P +RP M ++V+ L
Sbjct: 2731 VDEIVDPGIKGGYHAEALWRVVEVALQCLEPYSTYRPCMVDIVREL 2868
Score = 40.9 bits (83), Expect(6) = 1e-060
Identities = 15/31 (48%), Positives = 22/31 (70%)
Frame = -1 / +1
Query: 2132 LNLAGNQFGGNLPYSISTMPNLKYLNLNHNQ 2040
L+L+ N G +PY ++ M NL+ LNL+HNQ
Sbjct: 1495 LDLSSNNLKGAIPYFVTKMTNLQILNLSHNQ 1587
Score = 36.8 bits (74), Expect(6) = 1e-060
Identities = 16/35 (45%), Positives = 20/35 (57%)
Frame = -1 / +1
Query: 1997 LSELDLSFNSLTGDLPQSFTGLSSLKRMYLQNNQF 1893
L LDLS+N L G LP+S L LK +Y N +
Sbjct: 1627 LISLDLSYNDLDGRLPESIISLPHLKSLYFGCNPY 1731
Score = 35.4 bits (71), Expect(6) = 1e-060
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -1 / +1
Query: 1268 FDNLVGEGTFGRVYRAQFNDGKVLAIKKLDST 1173
+ L+ EG FG VYR +DG+ +A+K ST
Sbjct: 2056 YKTLICEGGFGSVYRGTLDDGQEVAVKVRSST 2151
>gb|CO511940.1|CO511940 s13dSG05G1200100_103780 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 595
Score = 133 bits (284), Expect(2) = 1e-051
Identities = 53/106 (50%), Positives = 69/106 (65%)
Frame = -1 / +1
Query: 776 GYTAPEVDMTGQYTFKSDVYSFGVVMLELLTGRRPFDSSRPRSEQSLVRWATPQLHDIDA 597
GY AP+ MTGQ TFKSD+YS GV +LEL+TGR+ D RP EQ+LV WA P D
Sbjct: 271 GYCAPDYAMTGQLTFKSDIYSIGVALLELITGRKAIDHRRPAKEQNLVAWARPLFRDRRR 450
Query: 596 LDRMVDPALKGLYPAKSLSRFADVLALCVQPEPEFRPPMSEVVQAL 459
M+DP L+G YP + L + + A+CVQ +P RP +++VV AL
Sbjct: 451 FSEMIDPLLEGQYPVRGLYQALAIAAMCVQEQPNMRPVIADVVTAL 588
Score = 86.8 bits (183), Expect(2) = 1e-051
Identities = 37/74 (50%), Positives = 49/74 (66%)
Frame = -1 / +1
Query: 1031 GSLHDLLHLSDEYSKPLSWNSRIKIALGSARALEYLHEICSPSIIHKNFKSSNILLDSEF 852
GSL + LH KPL WN+R+KIA G+AR LEYLH+ P +I+++ K SNILL ++
Sbjct: 4 GSLENHLHDLSPGQKPLDWNTRMKIAAGAARGLEYLHDKMKPPVIYRDLKCSNILLGEDY 183
Query: 851 NPHLSDAGLASFIP 810
+ LSD GLA P
Sbjct: 184 HSKLSDFGLAKVGP 225
>gb|CO512543.1|CO512543 s13dSG08D0400030_121012 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 611
Score = 78.5 bits (165), Expect(4) = 1e-034
Identities = 32/61 (52%), Positives = 44/61 (72%)
Frame = -1 / +1
Query: 983 LSWNSRIKIALGSARALEYLHEICSPSIIHKNFKSSNILLDSEFNPHLSDAGLASFIPGA 804
+SWN RIK+AL +AR +EYLH+ P IIH++ K+SNILLDS++ +SD GL+ P
Sbjct: 283 MSWNGRIKVALDAARGIEYLHKYAQPPIIHRDIKTSNILLDSKWVAKVSDFGLSLMGPED 462
Query: 803 E 801
E
Sbjct: 463 E 465
Score = 51.0 bits (105), Expect(4) = 1e-034
Identities = 19/46 (41%), Positives = 31/46 (67%)
Frame = -1 / +1
Query: 1145 FAELVSNISKLHHPNLNELVGYCMEHGQHLLVYDFHRNGSLHDLLH 1008
F + ++S+LHH NL +L+G+ + +LVY++ NGSL+D LH
Sbjct: 124 FVNELESLSRLHHKNLVKLLGFYEDKNGRILVYEYMNNGSLNDHLH 261
Score = 43.2 bits (88), Expect(4) = 1e-034
Identities = 20/37 (54%), Positives = 23/37 (62%)
Frame = -1 / +1
Query: 791 AEQSAGYTAPEVDMTGQYTFKSDVYSFGVVMLELLTG 681
A + GY PE T KSDVYSFGV +LELL+G
Sbjct: 487 AAGTVGYMDPEYYRLQYLTSKSDVYSFGVXLLELLSG 597
Score = 25.4 bits (49), Expect(4) = 1e-034
Identities = 9/17 (52%), Positives = 13/17 (76%)
Frame = -1 / +1
Query: 1229 YRAQFNDGKVLAIKKLD 1179
YRA+ DGK +AIK+ +
Sbjct: 4 YRAKLEDGKEVAIKRAE 54
>gb|AY563141.1| Medicago sativa calcium/calmodulin-regulated receptor-like kinase
mRNA, complete cds
Length = 1798
Score = 69.3 bits (145), Expect(3) = 4e-028
Identities = 30/66 (45%), Positives = 42/66 (63%)
Frame = -1 / +1
Query: 782 SAGYTAPEVDMTGQYTFKSDVYSFGVVMLELLTGRRPFDSSRPRSEQSLVRWATPQLHDI 603
+AGY PE T Q T KSDVYSFGV+++E++TGR P + + E+ +RWA L +
Sbjct: 1057 TAGYMDPEYLRTYQLTEKSDVYSFGVLLVEMMTGRHPVEPKKKIDERVTIRWAMKMLKNG 1236
Query: 602 DALDRM 585
DA+ M
Sbjct: 1237 DAVFAM 1254
Score = 51.9 bits (107), Expect(3) = 4e-028
Identities = 18/42 (42%), Positives = 29/42 (69%)
Frame = -1 / +1
Query: 1310 YSVADLQMATDSFSFDNLVGEGTFGRVYRAQFNDGKVLAIKK 1185
++ ++ +T FS DN +GEG FG VYR + NDG ++A+K+
Sbjct: 526 FTFEEIYKSTAKFSSDNQIGEGGFGTVYRGKLNDGTIVAVKR 651
Score = 47.3 bits (97), Expect(3) = 4e-028
Identities = 22/63 (34%), Positives = 31/63 (49%)
Frame = -1 / +1
Query: 1010 HLSDEYSKPLSWNSRIKIALGSARALEYLHEICSPSIIHKNFKSSNILLDSEFNPHLSDA 831
HL L R+ IA+ A A+ YLH IIH++ K+SNIL+ ++D
Sbjct: 817 HLDGIRGDGLEIGERLDIAIDIAHAITYLHMYTDNPIIHRDIKASNILISENLRAKVADF 996
Query: 830 GLA 822
G A
Sbjct: 997 GFA 1005
>gb|CO512977.1|CO512977 s13dSG09B0200013_121880 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 582
Score = 65.7 bits (137), Expect(3) = 7e-027
Identities = 25/54 (46%), Positives = 35/54 (64%)
Frame = -1 / +1
Query: 986 PLSWNSRIKIALGSARALEYLHEICSPSIIHKNFKSSNILLDSEFNPHLSDAGL 825
PL+W+ R+KI G+A+ L YLHE +H + K SNILL + P++SD GL
Sbjct: 172 PLTWSDRLKIMKGTAKGLVYLHEFSPKKYVHGDLKPSNILLGHDMTPYISDFGL 333
Score = 45.1 bits (92), Expect(3) = 7e-027
Identities = 18/36 (50%), Positives = 26/36 (72%)
Frame = -1 / +1
Query: 779 AGYTAPEVDMTGQYTFKSDVYSFGVVMLELLTGRRP 672
+GY APE + + K DVYS+GV++LE++TGR P
Sbjct: 454 SGYQAPEALKVVKPSQKWDVYSYGVILLEMITGRLP 561
Score = 43.7 bits (89), Expect(3) = 7e-027
Identities = 19/47 (40%), Positives = 25/47 (53%)
Frame = -1 / +1
Query: 1148 DFAELVSNISKLHHPNLNELVGYCMEHGQHLLVYDFHRNGSLHDLLH 1008
+F V I KL HPN+ L Y + LL+YD+ NGSL +H
Sbjct: 4 EFQTEVEAIGKLRHPNIATLRAYYWSVDEKLLIYDYIPNGSLASAIH 144
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 24,212,048
Number of Sequences: 7669
Number of extensions: 353045
Number of successful extensions: 2338
Number of sequences better than 1.0e-020: 11
length of database: 1,248,568
effective HSP length: 45
effective length of database: 903,463
effective search space used: 753488142
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)