TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2943819.2.1
(1121 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512415.1|CO512415 s13dSG80F0600047_114344 Glandular tr... 216 4e-057
gb|CO511707.1|CO511707 s13dSG01B0800073_103314 Glandular tr... 191 1e-049
gb|CO515771.1|CO515771 s13dSG77F1000091_419691 Glandular tr... 173 4e-044
gb|CO514833.1|CO514833 s13dSG59G1100088_328326 Glandular tr... 104 5e-027
gb|CO513208.1|CO513208 s13dSG24B1200093_129580 Glandular tr... 103 9e-026
>gb|CO512415.1|CO512415 s13dSG80F0600047_114344 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 565
Score = 216 bits (466), Expect = 4e-057
Identities = 82/115 (71%), Positives = 95/115 (82%)
Frame = -2 / +2
Query: 1120 GRSPCCEKAHTNRGAWTKEEDERLVAYIRAHGEGCWRSLPKAAGLLRCGKSCRLRWINYL 941
GR+PCCEK RG WT EE E L YI+A+GEG WRSLPK AGLLRCGKSCRLRWINYL
Sbjct: 167 GRTPCCEKVGLKRGRWTTEEGEILSKYIQANGEGSWRSLPKNAGLLRCGKSCRLRWINYL 346
Query: 940 RPDLKRGNFTADEDDLIVKLHSLLGNKWSLIAARLPGRTDNEIKNYWNTHVRRKL 776
R DLKRGN +A E+D IVKLH+ GN+WS+IA+ LPGRTDNEIKN+WN+H+ RK+
Sbjct: 347 RTDLKRGNISAQEEDTIVKLHTTYGNRWSMIASHLPGRTDNEIKNHWNSHLSRKI 511
Score = 103 bits (220), Expect = 4e-023
Identities = 56/108 (51%), Positives = 61/108 (56%)
Frame = +3 / -2
Query: 798 FQ*FLISLSVRPGSRAAMSDHLFPRRLCSLTMRSSSSAVKLPRFRSGRR*LIHRSRQLLP 977
FQ FLISLSV PG A+ DHL P + S TM SSS A P RS R*LIH S QL P
Sbjct: 489 FQWFLISLSVLPGKWLAIMDHLLP*VVWSFTMVSSS*ADMFPLLRSVLR*LIHLSLQLFP 310
Query: 978 HRSRPAALGSDRQHPSPCARM*ATXXXXXXLVHAPLLVCAFSQQGDRP 1121
H + PA G+D Q PSP A M +VH PL FSQQG P
Sbjct: 309 HLNNPAFFGNDLQEPSPLA*MYFDKISPSSVVHLPLFSPTFSQQGVLP 166
>gb|CO511707.1|CO511707 s13dSG01B0800073_103314 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 570
Score = 191 bits (412), Expect = 1e-049
Identities = 70/115 (60%), Positives = 86/115 (74%)
Frame = -2 / +1
Query: 1120 GRSPCCEKAHTNRGAWTKEEDERLVAYIRAHGEGCWRSLPKAAGLLRCGKSCRLRWINYL 941
GR PCC+K +G WT EED LV YI+ HG G WR++P GL RC KSCRLRW NYL
Sbjct: 16 GRPPCCDKEGVKKGPWTPEEDIILVTYIQEHGPGNWRAVPTKTGLSRCSKSCRLRWTNYL 195
Query: 940 RPDLKRGNFTADEDDLIVKLHSLLGNKWSLIAARLPGRTDNEIKNYWNTHVRRKL 776
RP +KRGNFT E+ +I+ L LLGN+W+ IA+ LP RTDN+IKNYWNTH+++KL
Sbjct: 196 RPGIKRGNFTEQEEKMIIHLQDLLGNRWAAIASYLPQRTDNDIKNYWNTHLKKKL 360
>gb|CO515771.1|CO515771 s13dSG77F1000091_419691 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 413
Score = 173 bits (372), Expect = 4e-044
Identities = 65/104 (62%), Positives = 84/104 (80%)
Frame = -2 / +3
Query: 1084 RGAWTKEEDERLVAYIRAHGEGCWRSLPKAAGLLRCGKSCRLRWINYLRPDLKRGNFTAD 905
+GAWT EED L A I +GEG W +PK AGL RC KSCRLRW+NYL P +KR +F+ D
Sbjct: 99 KGAWTYEEDNLLKACINKYGEGKWHLVPKRAGLNRCRKSCRLRWVNYLNPAIKRESFSHD 278
Query: 904 EDDLIVKLHSLLGNKWSLIAARLPGRTDNEIKNYWNTHVRRKLL 773
E D+I++LH LLGN+WSLIAARLPGRT N++KNYW+T++R+K++
Sbjct: 279 EIDMILRLHKLLGNRWSLIAARLPGRTANDVKNYWHTNLRKKVI 410
>gb|CO514833.1|CO514833 s13dSG59G1100088_328326 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 480
Score = 104 bits (221), Expect(2) = 5e-027
Identities = 41/82 (50%), Positives = 53/82 (64%)
Frame = -2 / +3
Query: 982 RCGKSCRLRWINYLRPDLKRGNFTADEDDLIVKLHSLLGNKWSLIAARLPGRTDNEIKNY 803
R GKSCRLRW N L P ++ FT +EDD I++ H+ GNKW+ IA L GRTDN IKN+
Sbjct: 153 RSGKSCRLRWCNQLSPQVEHRAFTPEEDDTIIRAHARFGNKWATIARLLSGRTDNAIKNH 332
Query: 802 WNTHVRRKLLGRGIDPVTHRPI 737
WN+ ++RK ID P+
Sbjct: 333 WNSTLKRKCSSIMIDDSESPPV 398
Score = 31.8 bits (63), Expect(2) = 5e-027
Identities = 11/30 (36%), Positives = 16/30 (53%)
Frame = -2 / +3
Query: 1084 RGAWTKEEDERLVAYIRAHGEGCWRSLPKA 995
+G W+ EEDE L + HG W + K+
Sbjct: 54 KGPWSPEEDEALQKLVEKHGPRNWSIISKS 143
>gb|CO513208.1|CO513208 s13dSG24B1200093_129580 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 598
Score = 103 bits (220), Expect(2) = 9e-026
Identities = 41/82 (50%), Positives = 53/82 (64%)
Frame = -2 / +3
Query: 982 RCGKSCRLRWINYLRPDLKRGNFTADEDDLIVKLHSLLGNKWSLIAARLPGRTDNEIKNY 803
R GKSCRLRW N L P ++ FT +EDD I++ H+ GNKW+ IA L GRTDN IKN+
Sbjct: 255 RSGKSCRLRWCNQLSPQVEHRAFTPEEDDTIIRAHARFGNKWATIARLLSGRTDNAIKNH 434
Query: 802 WNTHVRRKLLGRGIDPVTHRPI 737
WN+ ++RK ID P+
Sbjct: 435 WNSTLKRKCSSIMIDDSESPPL 500
Score = 28.1 bits (55), Expect(2) = 9e-026
Identities = 10/30 (33%), Positives = 15/30 (50%)
Frame = -2 / +3
Query: 1084 RGAWTKEEDERLVAYIRAHGEGCWRSLPKA 995
+G W+ EEDE L + H W + K+
Sbjct: 156 KGPWSPEEDEALQKLVEKHRPRNWSIISKS 245
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,291,590
Number of Sequences: 7669
Number of extensions: 69107
Number of successful extensions: 789
Number of sequences better than 1.0e-020: 6
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 303204330
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)