TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2850725.2.1
(847 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF000975.1|MSAF000975 Medicago sativa 7-O-methyltransfer... 113 2e-032
gb|U97125.1|MSU97125 Medicago sativa isoflavone-O-methytran... 113 2e-032
emb|AX050436.1| Sequence 1 from Patent WO0071736 113 2e-032
gb|AF000976.1|MSAF000976 Medicago sativa 7-O-methyltransfer... 111 9e-032
gb|AF023481.1|AF023481 Medicago sativa o-methytransferase (... 63 1e-023
gb|M63853.1|ALFSALMCAT Medicago sativa S-adenosyl-L-methion... 89 3e-021
emb|AX259373.1| Sequence 3 from Patent WO0173090 89 3e-021
gb|L10211.1|ALFIOM Medicago sativa isoliquiritigenin 2'-O-m... 88 1e-020
>gb|AF000975.1|MSAF000975 Medicago sativa 7-O-methyltransferase (7-IOMT(6)) mRNA, complete
cds
Length = 1284
Score = 113 bits (241), Expect(2) = 2e-032
Identities = 42/95 (44%), Positives = 62/95 (65%)
Frame = -1 / +3
Query: 832 GDGTTGNSLAKAFPLIKITVLDLPQEIRKIPADGVVNYVGGDMFKSIPRAQMVLLKMVLH 653
G GTT + + FP +K V D PQ + + + YVGGDMF SIP A VLLK +LH
Sbjct: 621 GTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNLTYVGGDMFTSIPNADAVLLKYILH 800
Query: 652 HWSDEDCVKILANCRKAIPSREEGGKVVIADIILD 548
+W+D+DC++IL C++A+ + + GKV I D+++D
Sbjct: 801 NWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVID 905
Score = 40.5 bits (82), Expect(2) = 2e-032
Identities = 16/43 (37%), Positives = 25/43 (58%)
Frame = -1 / +3
Query: 493 MLMKGGRQRDVNDWRDLIQKAGFSDYKLLKKFGARGVLEIYP* 365
M G++R+ +W+ L +AGF YK+ G ++EIYP*
Sbjct: 960 MACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1088
>gb|U97125.1|MSU97125 Medicago sativa isoflavone-O-methytransferase mRNA, complete cds
Length = 1231
Score = 113 bits (241), Expect(2) = 2e-032
Identities = 42/95 (44%), Positives = 62/95 (65%)
Frame = -1 / +1
Query: 832 GDGTTGNSLAKAFPLIKITVLDLPQEIRKIPADGVVNYVGGDMFKSIPRAQMVLLKMVLH 653
G GTT + + FP +K V D PQ + + + YVGGDMF SIP A VLLK +LH
Sbjct: 616 GTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNLTYVGGDMFTSIPNADAVLLKYILH 795
Query: 652 HWSDEDCVKILANCRKAIPSREEGGKVVIADIILD 548
+W+D+DC++IL C++A+ + + GKV I D+++D
Sbjct: 796 NWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVID 900
Score = 40.5 bits (82), Expect(2) = 2e-032
Identities = 16/43 (37%), Positives = 25/43 (58%)
Frame = -1 / +1
Query: 493 MLMKGGRQRDVNDWRDLIQKAGFSDYKLLKKFGARGVLEIYP* 365
M G++R+ +W+ L +AGF YK+ G ++EIYP*
Sbjct: 955 MACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1083
>emb|AX050436.1| Sequence 1 from Patent WO0071736
Length = 1231
Score = 113 bits (241), Expect(2) = 2e-032
Identities = 42/95 (44%), Positives = 62/95 (65%)
Frame = -1 / +1
Query: 832 GDGTTGNSLAKAFPLIKITVLDLPQEIRKIPADGVVNYVGGDMFKSIPRAQMVLLKMVLH 653
G GTT + + FP +K V D PQ + + + YVGGDMF SIP A VLLK +LH
Sbjct: 616 GTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNLTYVGGDMFTSIPNADAVLLKYILH 795
Query: 652 HWSDEDCVKILANCRKAIPSREEGGKVVIADIILD 548
+W+D+DC++IL C++A+ + + GKV I D+++D
Sbjct: 796 NWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVID 900
Score = 40.5 bits (82), Expect(2) = 2e-032
Identities = 16/43 (37%), Positives = 25/43 (58%)
Frame = -1 / +1
Query: 493 MLMKGGRQRDVNDWRDLIQKAGFSDYKLLKKFGARGVLEIYP* 365
M G++R+ +W+ L +AGF YK+ G ++EIYP*
Sbjct: 955 MACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1083
>gb|AF000976.1|MSAF000976 Medicago sativa 7-O-methyltransferase (7-IOMT(9)) mRNA, complete
cds
Length = 1227
Score = 111 bits (236), Expect(2) = 9e-032
Identities = 41/95 (43%), Positives = 62/95 (65%)
Frame = -1 / +3
Query: 832 GDGTTGNSLAKAFPLIKITVLDLPQEIRKIPADGVVNYVGGDMFKSIPRAQMVLLKMVLH 653
G GTT + + FP +K V D PQ + + + YVGGDMF SIP A VLLK +LH
Sbjct: 615 GTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNLTYVGGDMFTSIPNADAVLLKYILH 794
Query: 652 HWSDEDCVKILANCRKAIPSREEGGKVVIADIILD 548
+W+D+DC++IL C++A+ + + GKV I D++++
Sbjct: 795 NWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIN 899
Score = 40.5 bits (82), Expect(2) = 9e-032
Identities = 16/43 (37%), Positives = 25/43 (58%)
Frame = -1 / +3
Query: 493 MLMKGGRQRDVNDWRDLIQKAGFSDYKLLKKFGARGVLEIYP* 365
M G++R+ +W+ L +AGF YK+ G ++EIYP*
Sbjct: 954 MACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1082
>gb|AF023481.1|AF023481 Medicago sativa o-methytransferase (iomt2003) mRNA, complete cds
Length = 1194
Score = 63.4 bits (132), Expect(3) = 1e-023
Identities = 26/56 (46%), Positives = 32/56 (57%)
Frame = -1 / +1
Query: 832 GDGTTGNSLAKAFPLIKITVLDLPQEIRKIPADGVVNYVGGDMFKSIPRAQMVLLK 665
G GTT + + FP +K V D PQ + + + YVGGDMF SIP A VLLK
Sbjct: 619 GTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNLTYVGGDMFTSIPNADAVLLK 786
Score = 40.5 bits (82), Expect(3) = 1e-023
Identities = 16/43 (37%), Positives = 25/43 (58%)
Frame = -1 / +1
Query: 493 MLMKGGRQRDVNDWRDLIQKAGFSDYKLLKKFGARGVLEIYP* 365
M G++R+ +W+ L +AGF YK+ G ++EIYP*
Sbjct: 931 MACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1059
Score = 37.7 bits (76), Expect(3) = 1e-023
Identities = 12/31 (38%), Positives = 23/31 (74%)
Frame = -1 / +1
Query: 640 EDCVKILANCRKAIPSREEGGKVVIADIILD 548
+DC++IL C++A+ + + GKV I D+++D
Sbjct: 784 KDCLRILKKCKEAVTNDGKRGKVTIIDMVID 876
>gb|M63853.1|ALFSALMCAT Medicago sativa S-adenosyl-L-methionine: caffeic acid
3-0-methyltransferase mRNA, complete cds
Length = 1317
Score = 89.0 bits (188), Expect(2) = 3e-021
Identities = 36/79 (45%), Positives = 47/79 (59%)
Frame = -1 / +1
Query: 832 GDGTTGNSLAKAFPLIKITVLDLPQEIRKIPADGVVNYVGGDMFKSIPRAQMVLLKMVLH 653
G G N++ +P IK DLP I P+ V +VGGDMF SIP+A V +K + H
Sbjct: 658 GTGAVINTIVSKYPTIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSIPKADAVFMKWICH 837
Query: 652 HWSDEDCVKILANCRKAIP 596
WSDE C+K L NC +A+P
Sbjct: 838 DWSDEHCLKFLKNCYEALP 894
Score = 27.2 bits (53), Expect(2) = 3e-021
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = -1 / +1
Query: 481 GGRQRDVNDWRDLIQKAGFSDYKL 410
GG++R ++ DL + AGF +K+
Sbjct: 1006 GGKERTQKEFEDLAKGAGFQGFKV 1077
>emb|AX259373.1| Sequence 3 from Patent WO0173090
Length = 1098
Score = 89.0 bits (188), Expect(2) = 3e-021
Identities = 36/79 (45%), Positives = 47/79 (59%)
Frame = -1 / +1
Query: 832 GDGTTGNSLAKAFPLIKITVLDLPQEIRKIPADGVVNYVGGDMFKSIPRAQMVLLKMVLH 653
G G N++ +P IK DLP I P+ V +VGGDMF SIP+A V +K + H
Sbjct: 628 GTGAVINTIVSKYPTIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSIPKADAVFMKWICH 807
Query: 652 HWSDEDCVKILANCRKAIP 596
WSDE C+K L NC +A+P
Sbjct: 808 DWSDEHCLKFLKNCYEALP 864
Score = 27.2 bits (53), Expect(2) = 3e-021
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = -1 / +1
Query: 481 GGRQRDVNDWRDLIQKAGFSDYKL 410
GG++R ++ DL + AGF +K+
Sbjct: 976 GGKERTQKEFEDLAKGAGFQGFKV 1047
>gb|L10211.1|ALFIOM Medicago sativa isoliquiritigenin 2'-O-methyltransferase mRNA,
complete cds
Length = 1300
Score = 87.7 bits (185), Expect(2) = 1e-020
Identities = 34/78 (43%), Positives = 47/78 (60%)
Frame = -1 / +3
Query: 832 GDGTTGNSLAKAFPLIKITVLDLPQEIRKIPADGVVNYVGGDMFKSIPRAQMVLLKMVLH 653
G G + +PLIK DLPQ I P + +VGGDMF S+P+ ++LK V H
Sbjct: 684 GSGRNLELIISKYPLIKGINFDLPQVIENAPPLSGIEHVGGDMFASVPQGDAMILKAVCH 863
Query: 652 HWSDEDCVKILANCRKAI 599
+WSDE C++ L+NC KA+
Sbjct: 864 NWSDEKCIEFLSNCHKAL 917
Score = 26.7 bits (52), Expect(2) = 1e-020
Identities = 10/33 (30%), Positives = 19/33 (57%)
Frame = -1 / +3
Query: 508 MDVCMMLMKGGRQRDVNDWRDLIQKAGFSDYKL 410
+D M + GGR+R + L + +GFS +++
Sbjct: 1002 LDNLMFITVGGRERTEKQYEKLSKLSGFSKFQV 1100
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,002,608
Number of Sequences: 7669
Number of extensions: 112420
Number of successful extensions: 1050
Number of sequences better than 1.0e-020: 8
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 221426330
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)