TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2763110.2.1
(541 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 88 7e-031
gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular tr... 88 7e-031
gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 88 2e-030
gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular tr... 111 7e-026
gb|CO513758.1|CO513758 s13dSG15G1200088_140298 Glandular tr... 106 2e-024
gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase ... 68 5e-023
gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase ... 67 5e-023
gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase ... 64 6e-022
>gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-K1) mRNA, complete cds
Length = 1904
Score = 87.7 bits (185), Expect(2) = 7e-031
Identities = 33/77 (42%), Positives = 45/77 (58%)
Frame = +2 / +1
Query: 200 DFRGSSFELLPFGSGRRSCPAIAMGXXXXXXXXXXXXHCFDWQLPEGMVEEDIDMEETGQ 379
DF+GS+FE +PFGSGRRSCP + +G HCF W+LP+GM ++DM +
Sbjct: 1402 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSDVFG 1581
Query: 380 LAFRKMVPLCLVPIKRV 430
L + L +P KRV
Sbjct: 1582 LTAPRASRLIAIPTKRV 1632
Score = 60.2 bits (125), Expect(2) = 7e-031
Identities = 21/52 (40%), Positives = 28/52 (53%)
Frame = +2 / +1
Query: 29 PGTLLIPRETMRSCVIGGYDVLPGTRIFVNVWAMGRDPSIWDRPEEFSPERF 184
P L+ ET + GY + R+ +N WA+GRD + WD PE F P RF
Sbjct: 1228 PPIPLLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRF 1383
>gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 87.7 bits (185), Expect(2) = 7e-031
Identities = 33/77 (42%), Positives = 45/77 (58%)
Frame = +2 / +2
Query: 200 DFRGSSFELLPFGSGRRSCPAIAMGXXXXXXXXXXXXHCFDWQLPEGMVEEDIDMEETGQ 379
DF+GS+FE +PFGSGRRSCP + +G HCF W+LP+GM ++DM +
Sbjct: 248 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSDVFG 427
Query: 380 LAFRKMVPLCLVPIKRV 430
L + L +P KRV
Sbjct: 428 LTAPRASRLIAIPTKRV 478
Score = 60.2 bits (125), Expect(2) = 7e-031
Identities = 21/52 (40%), Positives = 28/52 (53%)
Frame = +2 / +2
Query: 29 PGTLLIPRETMRSCVIGGYDVLPGTRIFVNVWAMGRDPSIWDRPEEFSPERF 184
P L+ ET + GY + R+ +N WA+GRD + WD PE F P RF
Sbjct: 74 PPIPLLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRF 229
>gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-K10) mRNA, complete cds
Length = 1885
Score = 87.7 bits (185), Expect(2) = 2e-030
Identities = 33/77 (42%), Positives = 45/77 (58%)
Frame = +2 / +2
Query: 200 DFRGSSFELLPFGSGRRSCPAIAMGXXXXXXXXXXXXHCFDWQLPEGMVEEDIDMEETGQ 379
DF+GS+FE +PFGSGRRSCP + +G HCF W+LP+GM ++DM +
Sbjct: 1388 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSDVFG 1567
Query: 380 LAFRKMVPLCLVPIKRV 430
L + L +P KRV
Sbjct: 1568 LTAPRASRLIAIPTKRV 1618
Score = 58.3 bits (121), Expect(2) = 2e-030
Identities = 20/52 (38%), Positives = 28/52 (53%)
Frame = +2 / +2
Query: 29 PGTLLIPRETMRSCVIGGYDVLPGTRIFVNVWAMGRDPSIWDRPEEFSPERF 184
P L+ ET + GY + R+ +N WA+GRD + W+ PE F P RF
Sbjct: 1214 PPIPLLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWEEPESFKPSRF 1369
>gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 506
Score = 111 bits (237), Expect = 7e-026
Identities = 42/79 (53%), Positives = 53/79 (67%)
Frame = +2 / +2
Query: 5 QENFRIHPPGTLLIPRETMRSCVIGGYDVLPGTRIFVNVWAMGRDPSIWDRPEEFSPERF 184
+E R+HP LLIPRE+++ C I GYD+ TR+ VN WA+GRDP W E F PERF
Sbjct: 269 KETMRLHPSLPLLIPRESIKPCQINGYDIPAKTRVIVNAWAIGRDPRYWVDAESFKPERF 448
Query: 185 EGSHVDFRGSSFELLPFGS 241
S DF+G+ FE +PFGS
Sbjct: 449 LNSRFDFKGTDFEYIPFGS 505
>gb|CO513758.1|CO513758 s13dSG15G1200088_140298 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 473
Score = 106 bits (226), Expect = 2e-024
Identities = 37/93 (39%), Positives = 58/93 (62%)
Frame = +2 / +1
Query: 128 MGRDPSIWDRPEEFSPERFEGSHVDFRGSSFELLPFGSGRRSCPAIAMGXXXXXXXXXXX 307
MGRDP+IWD P F PERF ++++G++FEL+PFG+GRR CP + +
Sbjct: 4 MGRDPAIWDNPNPFVPERFMECDINYKGNNFELIPFGAGRRICPGLPLAHRTMHLMVASL 183
Query: 308 XHCFDWQLPEGMVEEDIDMEETGQLAFRKMVPL 406
H F+W L +G++ E ++M+E L +++ PL
Sbjct: 184 LHNFEWNLADGLIPEHLNMDEQFGLTLKRVQPL 282
>gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase 3 (ifs3) mRNA, partial cds
Length = 1501
Score = 67.5 bits (141), Expect(2) = 5e-023
Identities = 25/52 (48%), Positives = 30/52 (57%)
Frame = +2 / +3
Query: 29 PGTLLIPRETMRSCVIGGYDVLPGTRIFVNVWAMGRDPSIWDRPEEFSPERF 184
P ++ R+ C I GY + G I NVW +GRDP WDRP EF PERF
Sbjct: 1059 PPLPVVKRKCTEECEINGYVIPEGALILFNVWQVGRDPKYWDRPSEFRPERF 1214
Score = 53.8 bits (111), Expect(2) = 5e-023
Identities = 20/49 (40%), Positives = 25/49 (51%)
Frame = +2 / +3
Query: 185 EGSHVDFRGSSFELLPFGSGRRSCPAIAMGXXXXXXXXXXXXHCFDWQL 331
E +D RG F+LLPFGSGRR CP + + CFD Q+
Sbjct: 1236 EARPLDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQV 1382
>gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase 1 (ifs1) mRNA, partial cds
Length = 1501
Score = 67.0 bits (140), Expect(2) = 5e-023
Identities = 24/52 (46%), Positives = 30/52 (57%)
Frame = +2 / +3
Query: 29 PGTLLIPRETMRSCVIGGYDVLPGTRIFVNVWAMGRDPSIWDRPEEFSPERF 184
P ++ R+ C I GY + G + NVW +GRDP WDRP EF PERF
Sbjct: 1059 PPLPVVKRKCTEECEINGYVIPEGALVLFNVWQVGRDPKYWDRPSEFRPERF 1214
Score = 54.2 bits (112), Expect(2) = 5e-023
Identities = 20/49 (40%), Positives = 25/49 (51%)
Frame = +2 / +3
Query: 185 EGSHVDFRGSSFELLPFGSGRRSCPAIAMGXXXXXXXXXXXXHCFDWQL 331
E +D RG F+LLPFGSGRR CP + + CFD Q+
Sbjct: 1236 EAGPLDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQV 1382
>gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase 2 (ifs2) mRNA, partial cds
Length = 1501
Score = 63.8 bits (133), Expect(2) = 6e-022
Identities = 24/52 (46%), Positives = 29/52 (55%)
Frame = +2 / +3
Query: 29 PGTLLIPRETMRSCVIGGYDVLPGTRIFVNVWAMGRDPSIWDRPEEFSPERF 184
P ++ R+ C I GY + G I NVW +GRD WDRP EF PERF
Sbjct: 1059 PPLPVVKRKCTEECEINGYVIPEGALILFNVWQVGRDSKYWDRPSEFRPERF 1214
Score = 53.8 bits (111), Expect(2) = 6e-022
Identities = 20/49 (40%), Positives = 25/49 (51%)
Frame = +2 / +3
Query: 185 EGSHVDFRGSSFELLPFGSGRRSCPAIAMGXXXXXXXXXXXXHCFDWQL 331
E +D RG F+LLPFGSGRR CP + + CFD Q+
Sbjct: 1236 EARPLDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQV 1382
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,000,546
Number of Sequences: 7669
Number of extensions: 75128
Number of successful extensions: 2053
Number of sequences better than 1.0e-020: 8
length of database: 1,248,568
effective HSP length: 41
effective length of database: 934,139
effective search space used: 128911182
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)