TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2643622.2.1
(1067 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 97 9e-049
gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 99 9e-049
gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase ... 111 3e-044
gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase ... 111 4e-044
gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase ... 111 3e-043
gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible c... 132 2e-041
gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular tr... 140 2e-034
gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular tr... 86 1e-033
gb|CO513758.1|CO513758 s13dSG15G1200088_140298 Glandular tr... 106 4e-024
>gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-K1) mRNA, complete cds
Length = 1904
Score = 96.8 bits (205), Expect(3) = 9e-049
Identities = 39/95 (41%), Positives = 60/95 (63%)
Frame = -3 / +1
Query: 858 LRDKYDLSDDTVIGLLWDMITAGTDTTVISV*WAMAELLRNPRVQEKLQEELDRVVGRDR 679
L + L+ D + ++ D++ GT+T ++ W MAEL+++P +K+Q+EL VVG R
Sbjct: 976 LHNSIKLTKDNIKAIIMDVMFGGTETVASAIEWVMAELMKSPEDLKKVQQELAEVVGLSR 1155
Query: 678 VLSETDFPNLPYLQAVVKESLRLHPPTPLMLPHRA 574
+ E DF L YL+ +KE+LRLHPP PL+L A
Sbjct: 1156 QVEEPDFEKLTYLKCALKETLRLHPPIPLLLHETA 1260
Score = 86.3 bits (182), Expect(3) = 9e-049
Identities = 34/77 (44%), Positives = 48/77 (62%)
Frame = -3 / +1
Query: 432 DIKGADFRVLPFGAGRRVCPGAQLGINLVASMIGHMLHHFTWTLPSGTCPEDVSMMESPG 253
D KG++F +PFG+GRR CPG QLG+ + + H+LH FTW LP G P ++ M + G
Sbjct: 1402 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSDVFG 1581
Query: 252 LVTFMATPLQAVATPRL 202
L A+ L A+ T R+
Sbjct: 1582 LTAPRASRLIAIPTKRV 1632
Score = 44.1 bits (90), Expect(3) = 9e-049
Identities = 17/53 (32%), Positives = 23/53 (43%)
Frame = -3 / +1
Query: 591 MLPHRASASVKIAGYDIPKGXXXXXXXXXXARDPEVWDSPLEFRPERFLRENI 433
+L H + + GY IPK RD WD P F+P RFL+ +
Sbjct: 1240 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRFLKPGV 1398
>gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-K10) mRNA, complete cds
Length = 1885
Score = 98.7 bits (209), Expect(3) = 9e-049
Identities = 40/95 (42%), Positives = 61/95 (64%)
Frame = -3 / +2
Query: 858 LRDKYDLSDDTVIGLLWDMITAGTDTTVISV*WAMAELLRNPRVQEKLQEELDRVVGRDR 679
L + L+ D + ++ D++ GT+T ++ WAMAEL+++P +K+Q+EL VVG R
Sbjct: 962 LHNSIKLTKDNIKAIIMDVMFGGTETVASAIEWAMAELMKSPEDLKKVQQELAEVVGLSR 1141
Query: 678 VLSETDFPNLPYLQAVVKESLRLHPPTPLMLPHRA 574
+ E DF L YL+ +KE+LRLHPP PL+L A
Sbjct: 1142 QVEEPDFEKLTYLKCALKETLRLHPPIPLLLHETA 1246
Score = 86.3 bits (182), Expect(3) = 9e-049
Identities = 34/77 (44%), Positives = 48/77 (62%)
Frame = -3 / +2
Query: 432 DIKGADFRVLPFGAGRRVCPGAQLGINLVASMIGHMLHHFTWTLPSGTCPEDVSMMESPG 253
D KG++F +PFG+GRR CPG QLG+ + + H+LH FTW LP G P ++ M + G
Sbjct: 1388 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSDVFG 1567
Query: 252 LVTFMATPLQAVATPRL 202
L A+ L A+ T R+
Sbjct: 1568 LTAPRASRLIAIPTKRV 1618
Score = 42.3 bits (86), Expect(3) = 9e-049
Identities = 16/53 (30%), Positives = 23/53 (43%)
Frame = -3 / +2
Query: 591 MLPHRASASVKIAGYDIPKGXXXXXXXXXXARDPEVWDSPLEFRPERFLRENI 433
+L H + + GY IPK RD W+ P F+P RFL+ +
Sbjct: 1226 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWEEPESFKPSRFLKPGV 1384
>gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase 3 (ifs3) mRNA, partial cds
Length = 1501
Score = 111 bits (237), Expect(3) = 3e-044
Identities = 41/84 (48%), Positives = 64/84 (76%)
Frame = -3 / +3
Query: 840 LSDDTVIGLLWDMITAGTDTTVISV*WAMAELLRNPRVQEKLQEELDRVVGRDRVLSETD 661
++ D + GL+ D +AGTD+T ++ WA+AEL+ NP+V EK +EE+ VVG+DR++ E D
Sbjct: 825 ITKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEVYSVVGKDRLVDEVD 1004
Query: 660 FPNLPYLQAVVKESLRLHPPTPLM 589
NLPY++A+VKE+ R+HPP P++
Sbjct: 1005 TQNLPYIRAIVKETFRMHPPLPVV 1076
Score = 50.6 bits (104), Expect(3) = 3e-044
Identities = 20/49 (40%), Positives = 27/49 (55%)
Frame = -3 / +3
Query: 591 MLPHRASASVKIAGYDIPKGXXXXXXXXXXARDPEVWDSPLEFRPERFL 445
++ + + +I GY IP+G RDP+ WD P EFRPERFL
Sbjct: 1071 VVKRKCTEECEINGYVIPEGALILFNVWQVGRDPKYWDRPSEFRPERFL 1217
Score = 50.1 bits (103), Expect(3) = 3e-044
Identities = 16/41 (39%), Positives = 30/41 (73%)
Frame = -3 / +3
Query: 435 IDIKGADFRVLPFGAGRRVCPGAQLGINLVASMIGHMLHHF 313
+D++G F++LPFG+GRR+CPG L + +A+++ ++ F
Sbjct: 1248 LDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCF 1370
>gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase 1 (ifs1) mRNA, partial cds
Length = 1501
Score = 111 bits (236), Expect(3) = 4e-044
Identities = 40/84 (47%), Positives = 65/84 (77%)
Frame = -3 / +3
Query: 840 LSDDTVIGLLWDMITAGTDTTVISV*WAMAELLRNPRVQEKLQEELDRVVGRDRVLSETD 661
++ + + GL+ D+ +AGTD+T ++ WA+AEL+ NPRV +K +EE+ VVG+DR++ E D
Sbjct: 825 ITKEQIKGLVVDLFSAGTDSTAVATEWALAELINNPRVLQKAREEVYSVVGKDRLVDEVD 1004
Query: 660 FPNLPYLQAVVKESLRLHPPTPLM 589
NLPY++A+VKE+ R+HPP P++
Sbjct: 1005 TQNLPYIRAIVKETFRMHPPLPVV 1076
Score = 50.6 bits (104), Expect(3) = 4e-044
Identities = 20/49 (40%), Positives = 27/49 (55%)
Frame = -3 / +3
Query: 591 MLPHRASASVKIAGYDIPKGXXXXXXXXXXARDPEVWDSPLEFRPERFL 445
++ + + +I GY IP+G RDP+ WD P EFRPERFL
Sbjct: 1071 VVKRKCTEECEINGYVIPEGALVLFNVWQVGRDPKYWDRPSEFRPERFL 1217
Score = 50.1 bits (103), Expect(3) = 4e-044
Identities = 16/41 (39%), Positives = 30/41 (73%)
Frame = -3 / +3
Query: 435 IDIKGADFRVLPFGAGRRVCPGAQLGINLVASMIGHMLHHF 313
+D++G F++LPFG+GRR+CPG L + +A+++ ++ F
Sbjct: 1248 LDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCF 1370
>gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase 2 (ifs2) mRNA, partial cds
Length = 1501
Score = 111 bits (237), Expect(3) = 3e-043
Identities = 41/84 (48%), Positives = 64/84 (76%)
Frame = -3 / +3
Query: 840 LSDDTVIGLLWDMITAGTDTTVISV*WAMAELLRNPRVQEKLQEELDRVVGRDRVLSETD 661
++ D + GL+ D +AGTD+T ++ WA+AEL+ NP+V EK +EE+ VVG+DR++ E D
Sbjct: 825 ITKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEVYSVVGKDRLVDEVD 1004
Query: 660 FPNLPYLQAVVKESLRLHPPTPLM 589
NLPY++A+VKE+ R+HPP P++
Sbjct: 1005 TQNLPYIRAIVKETFRMHPPLPVV 1076
Score = 50.1 bits (103), Expect(3) = 3e-043
Identities = 16/41 (39%), Positives = 30/41 (73%)
Frame = -3 / +3
Query: 435 IDIKGADFRVLPFGAGRRVCPGAQLGINLVASMIGHMLHHF 313
+D++G F++LPFG+GRR+CPG L + +A+++ ++ F
Sbjct: 1248 LDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCF 1370
Score = 46.9 bits (96), Expect(3) = 3e-043
Identities = 19/49 (38%), Positives = 26/49 (53%)
Frame = -3 / +3
Query: 591 MLPHRASASVKIAGYDIPKGXXXXXXXXXXARDPEVWDSPLEFRPERFL 445
++ + + +I GY IP+G RD + WD P EFRPERFL
Sbjct: 1071 VVKRKCTEECEINGYVIPEGALILFNVWQVGRDSKYWDRPSEFRPERFL 1217
>gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible cinnamic acid 4-hydroxylase mRNA,
complete cds
Length = 1744
Score = 132 bits (283), Expect(2) = 2e-041
Identities = 56/151 (37%), Positives = 89/151 (58%)
Frame = -3 / +3
Query: 876 VDALFTLRDKYDLSDDTVIGLLWDMITAGTDTTVISV*WAMAELLRNPRVQEKLQEELDR 697
+D + + K +++DD V+ ++ ++ A +TT+ S+ W +AEL+ + +Q K++EE+DR
Sbjct: 867 IDHILDAQKKGEINDDNVLYIVENINVAAIETTLWSIEWGIAELVNHQDIQNKVREEMDR 1046
Query: 696 VVGRDRVLSETDFPNLPYLQAVVKESLRLHPPTPLMLPHRASASVKIAGYDIPKGXXXXX 517
V+G ++E D LPYLQAV+KE+LRL PL++PH K+ G+DIP
Sbjct: 1047 VLGPGHQVTEPDLHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDPKLNGFDIPAESKILV 1226
Query: 516 XXXXXARDPEVWDSPLEFRPERFLRENIDIK 424
+P W P EFRPERFL E ++
Sbjct: 1227 NAWWLPNNPAHWKKPEEFRPERFLEEESHVE 1319
Score = 51.5 bits (106), Expect(2) = 2e-041
Identities = 21/56 (37%), Positives = 30/56 (53%)
Frame = -3 / +3
Query: 438 NIDIKGADFRVLPFGAGRRVCPGAQLGINLVASMIGHMLHHFTWTLPSGTCPEDVS 271
+++ G DFR LPFG GRR CPG L + ++ IG ++ + P G D S
Sbjct: 1311 HVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNVELLPPPGQSKIDTS 1478
>gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 506
Score = 140 bits (301), Expect = 2e-034
Identities = 58/141 (41%), Positives = 81/141 (57%)
Frame = -3 / +2
Query: 813 LWDMITAGTDTTVISV*WAMAELLRNPRVQEKLQEELDRVVGRDRVLSETDFPNLPYLQA 634
L DM GT+++ V WAM+E+++NP V EK Q E+ RV + + ET+ L YL++
Sbjct: 83 LQDMFVGGTESSSEVVLWAMSEMVKNPMVMEKAQVEVRRVFDKKGYVDETELHQLIYLKS 262
Query: 633 VVKESLRLHPPTPLMLPHRASASVKIAGYDIPKGXXXXXXXXXXARDPEVWDSPLEFRPE 454
V+KE++RLHP PL++P + +I GYDIP RDP W F+PE
Sbjct: 263 VIKETMRLHPSLPLLIPRESIKPCQINGYDIPAKTRVIVNAWAIGRDPRYWVDAESFKPE 442
Query: 453 RFLRENIDIKGADFRVLPFGA 391
RFL D KG DF +PFG+
Sbjct: 443 RFLNSRFDFKGTDFEYIPFGS 505
>gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 86.3 bits (182), Expect(3) = 1e-033
Identities = 34/77 (44%), Positives = 48/77 (62%)
Frame = -3 / +2
Query: 432 DIKGADFRVLPFGAGRRVCPGAQLGINLVASMIGHMLHHFTWTLPSGTCPEDVSMMESPG 253
D KG++F +PFG+GRR CPG QLG+ + + H+LH FTW LP G P ++ M + G
Sbjct: 248 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSDVFG 427
Query: 252 LVTFMATPLQAVATPRL 202
L A+ L A+ T R+
Sbjct: 428 LTAPRASRLIAIPTKRV 478
Score = 46.4 bits (95), Expect(3) = 1e-033
Identities = 18/34 (52%), Positives = 23/34 (67%)
Frame = -3 / +2
Query: 675 LSETDFPNLPYLQAVVKESLRLHPPTPLMLPHRA 574
+ E DF L YL+ +KE+LRLHPP PL+L A
Sbjct: 5 VEEPDFEKLTYLKCALKETLRLHPPIPLLLHETA 106
Score = 43.2 bits (88), Expect(3) = 1e-033
Identities = 17/50 (34%), Positives = 22/50 (44%)
Frame = -3 / +2
Query: 591 MLPHRASASVKIAGYDIPKGXXXXXXXXXXARDPEVWDSPLEFRPERFLR 442
+L H + + GY IPK RD WD P F+P RFL+
Sbjct: 86 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRFLK 235
>gb|CO513758.1|CO513758 s13dSG15G1200088_140298 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 473
Score = 106 bits (227), Expect = 4e-024
Identities = 42/95 (44%), Positives = 58/95 (61%)
Frame = -3 / +1
Query: 498 RDPEVWDSPLEFRPERFLRENIDIKGADFRVLPFGAGRRVCPGAQLGINLVASMIGHMLH 319
RDP +WD+P F PERF+ +I+ KG +F ++PFGAGRR+CPG L + M+ +LH
Sbjct: 10 RDPAIWDNPNPFVPERFMECDINYKGNNFELIPFGAGRRICPGLPLAHRTMHLMVASLLH 189
Query: 318 HFTWTLPSGTCPEDVSMMESPGLVTFMATPLQAVA 214
+F W L G PE ++M E GL PL+ A
Sbjct: 190 NFEWNLADGLIPEHLNMDEQFGLTLKRVQPLRVEA 294
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,871,512
Number of Sequences: 7669
Number of extensions: 64676
Number of successful extensions: 693
Number of sequences better than 1.0e-020: 9
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 286665912
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)