TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2623104.2.3
(1540 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U20431.1|MSU20431 Medicago sativa polygalacturonase mRNA... 281 1e-097
gb|AY219848.1| Medicago sativa polygalactorunase PG11 precu... 215 3e-088
emb|Y11118.1|MSPOLGAL3 M.sativa PG3 gene 182 2e-076
>gb|U20431.1|MSU20431 Medicago sativa polygalacturonase mRNA, complete cds
Length = 1492
Score = 281 bits (607), Expect(2) = 1e-097
Identities = 108/218 (49%), Positives = 153/218 (70%)
Frame = +2 / +1
Query: 530 DFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGIHMGDSSGVTITN 709
+FVNN V G+T +SK FH+ ++ CK++ +TAPGDSPNTDGIHMG S+ V I N
Sbjct: 487 NFVNNSIVRGVTSKDSKNFHVMVFGCKNITFDGFTITAPGDSPNTDGIHMGKSTDVKILN 666
Query: 710 TVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTA 889
T IG GDDC+SIG G+ ++ + GV CGPGHG+S+GSLG++ E++V I VK+CTL T
Sbjct: 667 TNIGTGDDCVSIGDGSKQITVQGVNCGPGHGLSVGSLGKFTTEENVEGITVKNCTLTATD 846
Query: 890 NGVRIKAYEDAASVLTASKIHYENIKMEDSGYPIIIDMKYCPNKLCTANGASKVTVKDVT 1069
NGVRIK + DA +T S IH+E+I M + P+IID +Y P C+ SK+ + ++
Sbjct: 847 NGVRIKTWPDAPGTITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCSKKNPSKIKLSKIS 1026
Query: 1070 FKNITGTSSTPEAVNLLCSAKIPCTGVTMDDVNIKYSG 1183
FKN+ GTS T E V L+CS+ +PC GV +++V++K++G
Sbjct: 1027 FKNVKGTSGTAEGVVLICSSAVPCDGVELNNVDLKFNG 1140
Score = 160 bits (345), Expect = 3e-040
Identities = 90/206 (43%), Positives = 107/206 (51%)
Frame = -2 / -3
Query: 1149 TPVHGILAEQSRLTASGVEEVPVMFLKVTSLTVTLEAPLAVHNLLGQYFMSMMMG*PESS 970
TP HG Q R T S V EVP+ FL L + H G Y SMM G
Sbjct: 1106 TPSHGTALLQIRTTPSAVPEVPLTFLNEILLNFIFDGFFLEHWFHG*YSWSMMTGFLTFV 927
Query: 969 ILIFS*WILEAVSTEAASSYALMRTPLAVFLSVQSLTLISVTSFSSLYRPRLPMLMPWPG 790
++I S I E V AS L+ TPL+V + VQ LT+I T S + PRLP L P PG
Sbjct: 926 MVISSKCISETVIVPGASGQVLILTPLSVAVRVQFLTVIPSTFSSVVNFPRLPTLRP*PG 747
Query: 789 PQVTPVMFTLEVPGPMEMQSSPTPMTVLVMVTPDESPMWMPSVLGLSPGAVTLTSLISMS 610
PQ TP +P P+E QSSP PM VL ++T + PMW+PSVLGLSPGAV + +
Sbjct: 746 PQFTPWTVICLLPSPIETQSSPVPMLVLRILTSVDFPMWIPSVLGLSPGAVIVKPSNVIF 567
Query: 609 LHLYMFMWKNLELSSVIPDTSPLFTK 532
LH WK L V P T L TK
Sbjct: 566 LHPKTITWKFLLSLEVTPRTMELLTK 489
Score = 91.8 bits (194), Expect(2) = 1e-097
Identities = 39/119 (32%), Positives = 61/119 (51%)
Frame = +2 / +1
Query: 179 GNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGPLNFTGPCKGDVTIQVNGNLLAT 358
G +D +A+ AW AC T I+IP G + + + GPCK + +QV+G + A
Sbjct: 124 GKPNSDIGQALTSAWNEACASTTAAKIVIPAGTYQLNGIELKGPCKAPIELQVDGTIQAP 303
Query: 359 TDLSQYKDHGNWIEILRVDNLVITGKGKLDGQGPAVWSKNSCVKKYDCKILPNSLVMDF 535
D S K W + L +D+L ++GKG DGQG V+ K + + K + + M+F
Sbjct: 304 ADPSVIKGTEQWFKFLYMDHLTLSGKGVFDGQGATVYKKAAPASAWSGKNSNSKVFMNF 480
>gb|AY219848.1| Medicago sativa polygalactorunase PG11 precursor, gene, complete cds
Length = 2213
Score = 215 bits (465), Expect(3) = 3e-088
Identities = 87/166 (52%), Positives = 111/166 (66%)
Frame = +2 / +1
Query: 533 FVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGIHMGDSSGVTITNT 712
FVNN V G+T +SK FH+ + C + VTAPGDSPNTDGIHMG S+ + I NT
Sbjct: 1225 FVNNSMVHGLTSKDSKNFHVMVLGCNNFTFDGFTVTAPGDSPNTDGIHMGRSTDIKILNT 1404
Query: 713 VIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTAN 892
I GDDCIS+G G+ K+ + V CGPGHGIS+GSLG+Y E +V + VK+CTL T N
Sbjct: 1405 NIATGDDCISLGDGSRKITVQNVKCGPGHGISVGSLGKYTTEDNVEGVIVKNCTLTATQN 1584
Query: 893 GVRIKAYEDAASVLTASKIHYENIKMEDSGYPIIIDMKYCPNKLCT 1030
GVRIK + DA +T S IH+E+I M + P+IID +YCP C+
Sbjct: 1585 GVRIKTWPDAPGTITVSDIHFEDIIMNNVMNPVIIDQEYCPWNQCS 1722
Score = 124 bits (265), Expect(2) = 7e-032
Identities = 71/164 (43%), Positives = 85/164 (51%)
Frame = -2 / -1
Query: 1026 HNLLGQYFMSMMMG*PESSILIFS*WILEAVSTEAASSYALMRTPLAVFLSVQSLTLISV 847
H GQY SMM G ++I S I E V AS L+ TP V + VQ LT+
Sbjct: 1718 HWFHGQYSWSMMTGFMTLFMIISSKCISETVIVPGASGQVLILTPF*VAVRVQFLTITPS 1539
Query: 846 TSFSSLYRPRLPMLMPWPGPQVTPVMFTLEVPGPMEMQSSPTPMTVLVMVTPDESPMWMP 667
T S +Y PRLP L+P PGP T +P P E+QSSP M VL ++ + PMW+P
Sbjct: 1538 TLSSVVYFPRLPTLIP*PGPHFTFWTVIFLLPSPNEIQSSPVAMLVLRILISVDLPMWIP 1359
Query: 666 SVLGLSPGAVTLTSLISMSLHLYMFMWKNLELSSVIPDTSPLFT 535
SVLGLSPGAVT+ LH WK L V P T L T
Sbjct: 1358 SVLGLSPGAVTVKPSNVKLLHPKTITWKFLLSLEVRPWTMELLT 1227
Score = 90.4 bits (191), Expect(3) = 3e-088
Identities = 36/91 (39%), Positives = 52/91 (57%)
Frame = +2 / +2
Query: 203 KAVQEAWASACGGTGKQTILIPKGDFLVGPLNFTGPCKGDVTIQVNGNLLATTDLSQYKD 382
KA+ AW AC T I+IP G + + + GPCK + +QV+G + A D SQ K
Sbjct: 770 KAIGNAWKEACLSTTAAKIMIPGGTYRMNGVELKGPCKAPIEVQVDGTIQAPADPSQMKA 949
Query: 383 HGNWIEILRVDNLVITGKGKLDGQGPAVWSK 475
WI+ L +++L ++GKG DGQG V+ K
Sbjct: 950 ADQWIKFLYMEHLTLSGKGVFDGQGATVYKK 1042
Score = 63.4 bits (132), Expect(3) = 3e-088
Identities = 22/47 (46%), Positives = 35/47 (74%)
Frame = +2 / +3
Query: 1043 SKVTVKDVTFKNITGTSSTPEAVNLLCSAKIPCTGVTMDDVNIKYSG 1183
SK+ + ++FKNI GTS TPE V L+CS+ +PC GV +++V + ++G
Sbjct: 1851 SKIKLSKISFKNIQGTSGTPEGVVLICSSGVPCDGVELNNVGLTFNG 1991
Score = 33.6 bits (67), Expect(2) = 7e-032
Identities = 16/26 (61%), Positives = 16/26 (61%)
Frame = -2 / -2
Query: 1149 TPVHGILAEQSRLTASGVEEVPVMFL 1072
TP HG Q R T SGV EVP MFL
Sbjct: 1957 TPSHGTPLLQIRTTPSGVPEVP*MFL 1880
>emb|Y11118.1|MSPOLGAL3 M.sativa PG3 gene
Length = 2185
Score = 182 bits (392), Expect(3) = 2e-076
Identities = 73/167 (43%), Positives = 106/167 (63%)
Frame = +2 / +1
Query: 530 DFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGIHMGDSSGVTITN 709
+F+ + V IT +SK FH+N+ C + V +TAP S NTDGIH+G S+ V + N
Sbjct: 1201 NFLKHSIVRDITSKDSKNFHVNVLGCTNFTFDSVTITAPVTSINTDGIHIGRSTDVKVLN 1380
Query: 710 TVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTA 889
T I GDDC+S+G G+ ++ + V CGPGHGIS+GSLG+Y E+ V + VK+CT+ T
Sbjct: 1381 TNIATGDDCVSLGDGSRQITVQNVNCGPGHGISVGSLGKYPKEEAVEHVLVKNCTITNTD 1560
Query: 890 NGVRIKAYEDAASVLTASKIHYENIKMEDSGYPIIIDMKYCPNKLCT 1030
NGVRIK + + + +H+E+I M + P+IID +YCP C+
Sbjct: 1561 NGVRIKTWPSSPGTSPITDMHFEDIIMVNVLNPVIIDQEYCPWNQCS 1701
Score = 97.7 bits (207), Expect(3) = 5e-027
Identities = 55/133 (41%), Positives = 69/133 (51%)
Frame = -2 / -3
Query: 1026 HNLLGQYFMSMMMG*PESSILIFS*WILEAVSTEAASSYALMRTPLAVFLSVQSLTLISV 847
H GQY SM+ G +++I S I L+ TPL+VF+ VQ LT
Sbjct: 1697 H*FQGQYSWSMITGFRTLTMIISSKCISVIGDVPGELGQVLILTPLSVFVMVQFLTRTCS 1518
Query: 846 TSFSSLYRPRLPMLMPWPGPQVTPVMFTLEVPGPMEMQSSPTPMTVLVMVTPDESPMWMP 667
T+ S Y P+LP L+PWPGPQ T +P P E QSSP M VL T + PM +P
Sbjct: 1517 TASSFGYFPKLPTLIPWPGPQFTFWTVICLLPSPNETQSSPVAMFVLRTFTSVDLPMCIP 1338
Query: 666 SVLGLSPGAVTLT 628
SV L GAV +T
Sbjct: 1337 SVFMLVTGAVMVT 1299
Score = 93.6 bits (198), Expect(3) = 2e-076
Identities = 35/100 (35%), Positives = 53/100 (53%)
Frame = +2 / +3
Query: 206 AVQEAWASACGGTGKQTILIPKGDFLVGPLNFTGPCKGDVTIQVNGNLLATTDLSQYKDH 385
A AW +ACG T ++IP+G + + ++ GPCK + +QV+G + A + + D
Sbjct: 450 AFTNAWKAACGSTTASKVMIPRGVYKMHAVDVKGPCKAPIEVQVDGTIQAPQNPDELNDA 629
Query: 386 GNWIEILRVDNLVITGKGKLDGQGPAVWSKNSCVKKYDCK 505
WI+ VD ++GKG DG G W +N C K CK
Sbjct: 630 YQWIKFGYVDYFTLSGKGVFDGNGETAWRQNDCGKNSTCK 749
Score = 53.8 bits (111), Expect(3) = 2e-076
Identities = 20/47 (42%), Positives = 30/47 (63%)
Frame = +2 / +2
Query: 1043 SKVTVKDVTFKNITGTSSTPEAVNLLCSAKIPCTGVTMDDVNIKYSG 1183
SK+ + V FKNI GTS T + V L+CS PC V +++V + ++G
Sbjct: 1808 SKIKISKVIFKNIRGTSKTKDGVVLICSKSFPCDAVELNNVALTFNG 1948
Score = 33.6 bits (67), Expect(3) = 5e-027
Identities = 17/31 (54%), Positives = 19/31 (61%)
Frame = -2 / -2
Query: 1149 TPVHGILAEQSRLTASGVEEVPVMFLKVTSL 1057
T HG L R T S V EVP+MFLK+T L
Sbjct: 1914 TASHGKLLLHIRTTPSLVFEVPLMFLKITLL 1822
Score = 31.8 bits (63), Expect(3) = 5e-027
Identities = 15/42 (35%), Positives = 19/42 (45%)
Frame = -2 / -1
Query: 534 KSITSELGRILQSYFLTQEFLLHTAGPCPSSFPLPVMTRLST 409
K + + R+L F Q F L P PS PLP + ST
Sbjct: 778 KLFSFTMERLLHVEFFPQSFCLQAVSPLPSKTPLPDKVK*ST 653
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 10,964,137
Number of Sequences: 7669
Number of extensions: 147889
Number of successful extensions: 1012
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 426409776
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)