TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2619548.2.1
(691 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514103.1|CO514103 s13dSG75C1200086_156868 Glandular tr... 282 2e-077
gb|DQ122831.1| Medicago sativa clone C3 ribosomal protein L... 281 5e-077
gb|CO513365.1|CO513365 s13dSG38B0700057_129894 Glandular tr... 273 1e-074
gb|AW698393.1|AW698393 G240 glandular-haired subtracted cDN... 230 1e-061
gb|CO513652.1|CO513652 s13dSG17C0900066_140086 Glandular tr... 209 4e-055
gb|CO515624.1|CO515624 s13dSG47G0800068_418307 Glandular tr... 137 1e-033
>gb|CO514103.1|CO514103 s13dSG75C1200086_156868 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 505
Score = 282 bits (611), Expect = 2e-077
Identities = 118/135 (87%), Positives = 126/135 (93%)
Frame = +2 / +2
Query: 62 MAVPLLTQKIVKKRVKQFKRPHLDRYKCLKPSWRRPKGIDSRVRRKFKGCTLMPNIGYGS 241
MAVPLLT+KI+KKRVK+F RP DR C+K SWRRPKGIDSRVRRKFKG TLMPNIGYGS
Sbjct: 50 MAVPLLTKKIIKKRVKRFIRPQSDRKICVKQSWRRPKGIDSRVRRKFKGVTLMPNIGYGS 229
Query: 242 DKKTRHYLPNKFKKFVVHNVSELELLLMHNRTYCAEIAHNVSTRKRKEIVERAAQLDIVV 421
DKKTRHYLPN FKKFVVHNV +LELL+MHNRTYCAEIAHNVSTRKRKEIVERAAQLD+VV
Sbjct: 230 DKKTRHYLPNGFKKFVVHNVQDLELLMMHNRTYCAEIAHNVSTRKRKEIVERAAQLDVVV 409
Query: 422 TNKLARLRSQEDE*N 466
TNKLARLRSQEDE*+
Sbjct: 410 TNKLARLRSQEDE*S 454
Score = 121 bits (258), Expect(2) = 2e-031
Identities = 58/127 (45%), Positives = 77/127 (60%)
Frame = -1 / -1
Query: 505 IFNTSRAQSKLLNISLVLLAAEPGELVGDHDVQLRSTLHNLLALAGGHVVGDLSAVSPVV 326
I + S+ L SLV L A+PGELVG++++QL T ++LL+ G H+V DLS V PVV
Sbjct: 493 INSKSKNNLTLQKRSLVFLTAQPGELVGNNNIQLSCTFNDLLSFPG*HIVCDLSTVCPVV 314
Query: 325 HQQQLQLGDIVNNKLFEXXXXXXXXXXXGAISDVGHQGASLELTPDAGVDTLGPAPAWLK 146
H Q+ Q+ DIV+NKL E AIS+V HQ S ELTP+ G++TL P PA L
Sbjct: 313 HHQKFQILDIVHNKLLETIRQIVAGLLV*AISNVWHQSNSFELTPNTGINTLWPTPALLD 134
Query: 145 ALVAVKM 125
V +
Sbjct: 133 TYFPVTL 113
Score = 29.0 bits (57), Expect(2) = 2e-031
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = -2 / -2
Query: 123 GLLNCLTLFFTIFCVSSGTA 64
GL+N LT F IF V+ GTA
Sbjct: 111 GLMNLLTRFLIIFLVNRGTA 52
>gb|DQ122831.1| Medicago sativa clone C3 ribosomal protein L32 mRNA, complete cds
Length = 558
Score = 281 bits (609), Expect = 5e-077
Identities = 119/135 (88%), Positives = 125/135 (92%)
Frame = +2 / +1
Query: 59 KMAVPLLTQKIVKKRVKQFKRPHLDRYKCLKPSWRRPKGIDSRVRRKFKGCTLMPNIGYG 238
KMAVPLLT+KIVKKRVK+F RP R C+K SWRRPKGIDSRVRRKFKG TLMPNIGYG
Sbjct: 58 KMAVPLLTKKIVKKRVKRFIRPQSGRKICVKQSWRRPKGIDSRVRRKFKGVTLMPNIGYG 237
Query: 239 SDKKTRHYLPNKFKKFVVHNVSELELLLMHNRTYCAEIAHNVSTRKRKEIVERAAQLDIV 418
SDKKTRHYLPN FKKFVVHNV +LELL+MHNRTYCAEIAHNVSTRKRKEIVERAAQLD+V
Sbjct: 238 SDKKTRHYLPNGFKKFVVHNVQDLELLMMHNRTYCAEIAHNVSTRKRKEIVERAAQLDVV 417
Query: 419 VTNKLARLRSQEDE* 463
VTNKLARLRSQEDE*
Sbjct: 418 VTNKLARLRSQEDE* 462
Score = 110 bits (235), Expect(2) = 2e-031
Identities = 52/123 (42%), Positives = 78/123 (63%)
Frame = -1 / -1
Query: 517 NLSNIFNTSRAQSKLLNISLVLLAAEPGELVGDHDVQLRSTLHNLLALAGGHVVGDLSAV 338
++ I S+ L SLV L A+PG+LVGD+++QL T ++LL+ +G H+V +LS V
Sbjct: 516 HVHTITTKSKNDLTLQKQSLVFLTAQPGQLVGDNNIQLCCTFNDLLSFSG*HIVCNLSTV 337
Query: 337 SPVVHQQQLQLGDIVNNKLFEXXXXXXXXXXXGAISDVGHQGASLELTPDAGVDTLGPAP 158
PVVH Q+LQ+ DIV+++L E A+S+V HQ S E +P++ ++TL PAP
Sbjct: 336 CPVVHHQKLQVLDIVHDELLESIRQIVAGLLV*AVSNVRHQSNSFEFSPNS*INTLWPAP 157
Query: 157 AWL 149
A L
Sbjct: 156 ALL 148
Score = 59.7 bits (124), Expect(2) = 1e-021
Identities = 26/32 (81%), Positives = 27/32 (84%)
Frame = -2 / -2
Query: 267 GR*CLVFLSEPYPMLGIKVHPLNLRLTRESIP 172
G+* VFLSEPYPMLGIKV PLN LTRESIP
Sbjct: 266 GK*WRVFLSEPYPMLGIKVTPLNFLLTRESIP 171
Score = 57.4 bits (119), Expect(2) = 1e-021
Identities = 27/34 (79%), Positives = 28/34 (82%)
Frame = -2 / -2
Query: 423 VTTMSSCAARSTISLRLRVDTLWAISAQ*VLLCI 322
VTT S+CAARSTIS RVDTL AISAQ VLLCI
Sbjct: 422 VTTTSNCAARSTISFLFRVDTLCAISAQYVLLCI 321
Score = 39.6 bits (80), Expect(2) = 2e-031
Identities = 17/25 (68%), Positives = 20/25 (80%)
Frame = -2 / -2
Query: 123 GLLNCLTLFFTIFCVSSGTAIFANL 49
GL+N LT FFTIF VS+GTAI + L
Sbjct: 122 GLMNLLTRFFTIFLVSNGTAILSLL 48
>gb|CO513365.1|CO513365 s13dSG38B0700057_129894 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 489
Score = 273 bits (591), Expect = 1e-074
Identities = 116/135 (85%), Positives = 122/135 (90%)
Frame = +2 / +1
Query: 59 KMAVPLLTQKIVKKRVKQFKRPHLDRYKCLKPSWRRPKGIDSRVRRKFKGCTLMPNIGYG 238
KM PL T+KIVKKRVK+ RP DR C+K SWRRPKGIDSRVRRKFKG TLMPNIGYG
Sbjct: 52 KMTGPLPTKKIVKKRVKRVIRPQSDRKICVKQSWRRPKGIDSRVRRKFKGVTLMPNIGYG 231
Query: 239 SDKKTRHYLPNKFKKFVVHNVSELELLLMHNRTYCAEIAHNVSTRKRKEIVERAAQLDIV 418
SDKKTRHYLPN FKKFVVHNV +LELL+MHNRTYCAEIAHNVSTRKRKEIVERAAQLD+V
Sbjct: 232 SDKKTRHYLPNGFKKFVVHNVQDLELLMMHNRTYCAEIAHNVSTRKRKEIVERAAQLDVV 411
Query: 419 VTNKLARLRSQEDE* 463
VTNKLARLRSQEDE*
Sbjct: 412 VTNKLARLRSQEDE* 456
Score = 108 bits (230), Expect = 9e-025
Identities = 49/113 (43%), Positives = 73/113 (64%)
Frame = -1 / -1
Query: 463 SLVLLAAEPGELVGDHDVQLRSTLHNLLALAGGHVVGDLSAVSPVVHQQQLQLGDIVNNK 284
SLV L A+PG+LVGD+++QL T ++LL+ +G H+V +LS V PVVH Q+ Q+ DIV++K
Sbjct: 456 SLVFLTAQPGQLVGDNNIQLCRTFNDLLSFSG*HIVCNLSTVCPVVHHQKFQVLDIVHDK 277
Query: 283 LFEXXXXXXXXXXXGAISDVGHQGASLELTPDAGVDTLGPAPAWLKALVAVKM 125
L E A+S+V HQ S + +P++ ++TL PAPA L V +
Sbjct: 276 LLESIRQIVAGLLV*AVSNVRHQSNSFKFSPNS*INTLWPAPALLHTYFPVTL 118
>gb|AW698393.1|AW698393 G240 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 405
Score = 230 bits (497), Expect = 1e-061
Identities = 94/115 (81%), Positives = 102/115 (88%)
Frame = +2 / +1
Query: 77 LTQKIVKKRVKQFKRPHLDRYKCLKPSWRRPKGIDSRVRRKFKGCTLMPNIGYGSDKKTR 256
LT+KI+K RVK+F RP DR C+K SWRRPKGIDS VRRKFKG TLMPNIGYGSDKKTR
Sbjct: 61 LTKKIIKNRVKRFIRPQSDRKICVKQSWRRPKGIDSHVRRKFKGVTLMPNIGYGSDKKTR 240
Query: 257 HYLPNKFKKFVVHNVSELELLLMHNRTYCAEIAHNVSTRKRKEIVERAAQLDIVV 421
HYLPN FKKFVVHNV +LELL+MHNRTYCAEIAHNVSTR+RKEIVERAA L +VV
Sbjct: 241 HYLPNGFKKFVVHNVQDLELLMMHNRTYCAEIAHNVSTRRRKEIVERAAHLGVVV 405
>gb|CO513652.1|CO513652 s13dSG17C0900066_140086 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 351
Score = 209 bits (450), Expect = 4e-055
Identities = 84/99 (84%), Positives = 90/99 (90%)
Frame = +2 / +1
Query: 62 MAVPLLTQKIVKKRVKQFKRPHLDRYKCLKPSWRRPKGIDSRVRRKFKGCTLMPNIGYGS 241
MAVPLLT+KI+KKRVK+F RP DR C+K SWRRPKGIDSRVRRKFKG TLMPNIGYGS
Sbjct: 55 MAVPLLTKKIIKKRVKRFIRPQSDRKICVKQSWRRPKGIDSRVRRKFKGVTLMPNIGYGS 234
Query: 242 DKKTRHYLPNKFKKFVVHNVSELELLLMHNRTYCAEIAH 358
DKKTRHYLPN FKKFVVHNV +LELL+MHNRTYCAEIAH
Sbjct: 235 DKKTRHYLPNGFKKFVVHNVQDLELLMMHNRTYCAEIAH 351
>gb|CO515624.1|CO515624 s13dSG47G0800068_418307 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 349
Score = 137 bits (294), Expect = 1e-033
Identities = 59/64 (92%), Positives = 62/64 (96%)
Frame = +2 / +2
Query: 278 KKFVVHNVSELELLLMHNRTYCAEIAHNVSTRKRKEIVERAAQLDIVVTNKLARLRSQED 457
KKFVVHNV +LELL+MHNRTYCAEIAHNVSTRKRKEIVERAAQLD+VVTNKLARLRSQED
Sbjct: 5 KKFVVHNVQDLELLMMHNRTYCAEIAHNVSTRKRKEIVERAAQLDVVVTNKLARLRSQED 184
Query: 458 E*NV 469
E* V
Sbjct: 185 E*LV 196
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,170,172
Number of Sequences: 7669
Number of extensions: 59471
Number of successful extensions: 932
Number of sequences better than 1.0e-020: 6
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 173249890
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)