TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2591297.2.1
(786 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO516727.1|CO516727 s13dSG74F0500044_446782 Glandular tr... 222 9e-066
gb|CO516904.1|CO516904 s13dSG58C0700052_468354 Glandular tr... 80 2e-030
>gb|CO516727.1|CO516727 s13dSG74F0500044_446782 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 561
Score = 222 bits (479), Expect(3) = 9e-066
Identities = 80/103 (77%), Positives = 91/103 (88%)
Frame = -2 / +1
Query: 704 LGVRYPFSQPMKLYSSLWNADDWATRGGREKTDWSNAPFVASYRGFHVDGCEASAEARFC 525
LGV+YPFSQPMK+Y+SLWNADDWATRGG EKTDWS APF+ASY+GFH+DGCEAS A+FC
Sbjct: 184 LGVKYPFSQPMKIYNSLWNADDWATRGGLEKTDWSKAPFIASYKGFHIDGCEASVNAKFC 363
Query: 524 ATQGARWWDQPEFRDLDAAQYRRLADVRRRYTIYNYCTDRDRY 396
TQG RWWDQPEF DLD AQ++RL VR++YTIYNYC DR RY
Sbjct: 364 DTQGKRWWDQPEFHDLDVAQWQRLRWVRQKYTIYNYCNDRKRY 492
Score = 106 bits (227), Expect(2) = 7e-027
Identities = 50/72 (69%), Positives = 52/72 (72%)
Frame = +1 / -2
Query: 487 NSGWSHQRAPWVAQKRASAEASQPSTWKPR*EATKGALDQSVFSRPPRVAQSSAFHRLE* 666
NSG SH P V+Q A EASQPS P *EA KGALDQSVFS PP VAQSSAFH+L
Sbjct: 401 NSG*SHHLFPCVSQNFAFTEASQPSI*NPL*EAMKGALDQSVFSNPPLVAQSSAFHKLLY 222
Query: 667 SFIGWLNGYRTP 702
FIGWLNGY TP
Sbjct: 221 IFIGWLNGYFTP 186
Score = 35.9 bits (72), Expect(3) = 9e-066
Identities = 13/15 (86%), Positives = 14/15 (93%)
Frame = -2 / +1
Query: 761 IAFFVDDVPIRVFKN 717
I FFVDD+PIRVFKN
Sbjct: 130 IVFFVDDIPIRVFKN 174
Score = 28.1 bits (55), Expect(2) = 7e-027
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = +1 / -2
Query: 712 LVFLNTRIGTSSTKNAI 762
L+FLNT IG SSTK I
Sbjct: 179 LMFLNTLIGISSTKKTI 129
Score = 25.8 bits (50), Expect(3) = 9e-066
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -2 / +1
Query: 386 VPPECARDRDV* 351
VP EC RDRD+*
Sbjct: 499 VPQECTRDRDI* 534
>gb|CO516904.1|CO516904 s13dSG58C0700052_468354 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 478
Score = 80.3 bits (169), Expect(3) = 2e-030
Identities = 28/50 (56%), Positives = 37/50 (74%)
Frame = -2 / +1
Query: 701 GVRYPFSQPMKLYSSLWNADDWATRGGREKTDWSNAPFVASYRGFHVDGC 552
G+ YP Q M ++S+LW AD+WATRGG EK +WS APF A Y+ F ++GC
Sbjct: 151 GIPYPKIQAMGVFSTLWEADNWATRGGLEKINWSKAPFYAYYKDFDIEGC 300
Score = 52.4 bits (108), Expect(3) = 2e-030
Identities = 18/44 (40%), Positives = 26/44 (59%)
Frame = -2 / +1
Query: 527 CATQGARWWDQPEFRDLDAAQYRRLADVRRRYTIYNYCTDRDRY 396
CAT WW++ E++ L A + RR VR + Y+YC D+ RY
Sbjct: 322 CATNPKNWWERVEYQALSAIEARRYRWVRMNHVTYDYCQDKSRY 453
Score = 31.8 bits (63), Expect(3) = 2e-030
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = -2 / +1
Query: 761 IAFFVDDVPIRVFKNTSA 708
I F+VD+ PIRV+KN A
Sbjct: 94 IVFYVDEAPIRVYKNNEA 147
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,212,200
Number of Sequences: 7669
Number of extensions: 69651
Number of successful extensions: 1003
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 202896930
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)