TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2591258.2.1
(819 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|CQ760964.1| Sequence 9 from Patent WO2004002216 151 1e-055
gb|U20736.1|MSU20736 Medicago sativa S-adenosyl-L-methionin... 151 1e-055
emb|AX259371.1| Sequence 1 from Patent WO0173090 151 1e-055
emb|CQ760958.1| Sequence 3 from Patent WO2004002216 151 1e-055
>emb|CQ760964.1| Sequence 9 from Patent WO2004002216
Length = 1906
Score = 151 bits (325), Expect(4) = 1e-055
Identities = 59/88 (67%), Positives = 72/88 (81%)
Frame = -3 / +1
Query: 565 QIGSPVIEKAGVAHKIDFRVGLALPVLDQMVSEEGNKGKFDFAFVDADKVNFLNYHERLL 386
++G PVI+KAGV HKIDFR G ALPVLD+M+ +E N G +DF FVDADK N+LNYH+RL+
Sbjct: 1327 ELGLPVIKKAGVDHKIDFREGPALPVLDEMIKDEKNHGSYDFIFVDADKDNYLNYHKRLI 1506
Query: 385 QLLRVGGLIAYDNTLWGGSVAAPHDEPL 302
L++VGG+I YDNTLW GSV AP D PL
Sbjct: 1507 DLVKVGGVIGYDNTLWNGSVVAPPDAPL 1590
Score = 85.8 bits (181), Expect(2) = 9e-021
Identities = 45/78 (57%), Positives = 49/78 (62%)
Frame = +3 / -3
Query: 327 TDPPHSVLS*AMRPPTLXXXXXXXW*LRKFTLSASTNAKSNLPLFPSSETIWSSTGSASP 506
TDP H VLS + PPTL W*LR +LSAST KS LP F SS I S TG A P
Sbjct: 1565 TDPFHKVLSYPITPPTLTRSINLLW*LR*LSLSASTKMKS*LP*FFSSLIISSRTGRAGP 1386
Query: 507 TRKSILCATPAFSITGDP 560
+ KSIL +TPAF ITG P
Sbjct: 1385 SLKSIL*STPAFLITGRP 1332
Score = 40.0 bits (81), Expect(4) = 1e-055
Identities = 13/22 (59%), Positives = 21/22 (95%)
Frame = -3 / +1
Query: 631 TALALPDDGKIVAIDVTRESYD 566
TALA+P+DGKI+A+D+ +E+Y+
Sbjct: 1264 TALAIPEDGKILAMDINKENYE 1329
Score = 39.1 bits (79), Expect(4) = 1e-055
Identities = 18/47 (38%), Positives = 23/47 (48%)
Frame = -2 / +1
Query: 776 IRHEHRTHPMAGMAASPDXXXXXXXXXXXLGAKNAIEVGVFTGYSLL 636
+R HP M S D + AKN +E+GV+TGYSLL
Sbjct: 1120 LREVTAKHPWNIMTTSADEGQFLSMLLKLINAKNTMEIGVYTGYSLL 1260
Score = 36.8 bits (74), Expect(4) = 1e-055
Identities = 9/21 (42%), Positives = 16/21 (76%)
Frame = -3 / +1
Query: 241 DPRVHVCQVAIADGLXLCRRV 179
DPR+ +C + + DG+ +CRR+
Sbjct: 1651 DPRIEICMLPVGDGITICRRI 1713
Score = 28.6 bits (56), Expect(2) = 9e-021
Identities = 15/28 (53%), Positives = 18/28 (64%)
Frame = +3 / -3
Query: 567 S*LSRVTSMATILPSSGRARAVNEQRVP 650
S* S + S+A I PSSG ARAV + P
Sbjct: 1328 S*FSLLMSIAKIFPSSGIARAVARRE*P 1245
>gb|U20736.1|MSU20736 Medicago sativa S-adenosyl-L-methionine:trans-caffeoyl-CoA
3-O-methyltransferase (CCOMT) mRNA, complete cds
Length = 966
Score = 151 bits (325), Expect(4) = 1e-055
Identities = 59/88 (67%), Positives = 72/88 (81%)
Frame = -3 / +3
Query: 565 QIGSPVIEKAGVAHKIDFRVGLALPVLDQMVSEEGNKGKFDFAFVDADKVNFLNYHERLL 386
++G PVI+KAGV HKIDFR G ALPVLD+M+ +E N G +DF FVDADK N+LNYH+RL+
Sbjct: 387 ELGLPVIKKAGVDHKIDFREGPALPVLDEMIKDEKNHGSYDFIFVDADKDNYLNYHKRLI 566
Query: 385 QLLRVGGLIAYDNTLWGGSVAAPHDEPL 302
L++VGG+I YDNTLW GSV AP D PL
Sbjct: 567 DLVKVGGVIGYDNTLWNGSVVAPPDAPL 650
Score = 85.8 bits (181), Expect(2) = 9e-021
Identities = 45/78 (57%), Positives = 49/78 (62%)
Frame = +3 / -3
Query: 327 TDPPHSVLS*AMRPPTLXXXXXXXW*LRKFTLSASTNAKSNLPLFPSSETIWSSTGSASP 506
TDP H VLS + PPTL W*LR +LSAST KS LP F SS I S TG A P
Sbjct: 625 TDPFHKVLSYPITPPTLTRSINLLW*LR*LSLSASTKMKS*LP*FFSSLIISSRTGRAGP 446
Query: 507 TRKSILCATPAFSITGDP 560
+ KSIL +TPAF ITG P
Sbjct: 445 SLKSIL*STPAFLITGRP 392
Score = 40.0 bits (81), Expect(4) = 1e-055
Identities = 13/22 (59%), Positives = 21/22 (95%)
Frame = -3 / +3
Query: 631 TALALPDDGKIVAIDVTRESYD 566
TALA+P+DGKI+A+D+ +E+Y+
Sbjct: 324 TALAIPEDGKILAMDINKENYE 389
Score = 39.1 bits (79), Expect(4) = 1e-055
Identities = 18/47 (38%), Positives = 23/47 (48%)
Frame = -2 / +3
Query: 776 IRHEHRTHPMAGMAASPDXXXXXXXXXXXLGAKNAIEVGVFTGYSLL 636
+R HP M S D + AKN +E+GV+TGYSLL
Sbjct: 180 LREVTAKHPWNIMTTSADEGQFLSMLLKLINAKNTMEIGVYTGYSLL 320
Score = 36.8 bits (74), Expect(4) = 1e-055
Identities = 9/21 (42%), Positives = 16/21 (76%)
Frame = -3 / +3
Query: 241 DPRVHVCQVAIADGLXLCRRV 179
DPR+ +C + + DG+ +CRR+
Sbjct: 711 DPRIEICMLPVGDGITICRRI 773
Score = 28.6 bits (56), Expect(2) = 9e-021
Identities = 15/28 (53%), Positives = 18/28 (64%)
Frame = +3 / -3
Query: 567 S*LSRVTSMATILPSSGRARAVNEQRVP 650
S* S + S+A I PSSG ARAV + P
Sbjct: 388 S*FSLLMSIAKIFPSSGIARAVARRE*P 305
>emb|AX259371.1| Sequence 1 from Patent WO0173090
Length = 744
Score = 151 bits (325), Expect(4) = 1e-055
Identities = 59/88 (67%), Positives = 72/88 (81%)
Frame = -3 / +1
Query: 565 QIGSPVIEKAGVAHKIDFRVGLALPVLDQMVSEEGNKGKFDFAFVDADKVNFLNYHERLL 386
++G PVI+KAGV HKIDFR G ALPVLD+M+ +E N G +DF FVDADK N+LNYH+RL+
Sbjct: 352 ELGLPVIKKAGVDHKIDFREGPALPVLDEMIKDEKNHGSYDFIFVDADKDNYLNYHKRLI 531
Query: 385 QLLRVGGLIAYDNTLWGGSVAAPHDEPL 302
L++VGG+I YDNTLW GSV AP D PL
Sbjct: 532 DLVKVGGVIGYDNTLWNGSVVAPPDAPL 615
Score = 85.8 bits (181), Expect(2) = 9e-021
Identities = 45/78 (57%), Positives = 49/78 (62%)
Frame = +3 / -2
Query: 327 TDPPHSVLS*AMRPPTLXXXXXXXW*LRKFTLSASTNAKSNLPLFPSSETIWSSTGSASP 506
TDP H VLS + PPTL W*LR +LSAST KS LP F SS I S TG A P
Sbjct: 590 TDPFHKVLSYPITPPTLTRSINLLW*LR*LSLSASTKMKS*LP*FFSSLIISSRTGRAGP 411
Query: 507 TRKSILCATPAFSITGDP 560
+ KSIL +TPAF ITG P
Sbjct: 410 SLKSIL*STPAFLITGRP 357
Score = 40.0 bits (81), Expect(4) = 1e-055
Identities = 13/22 (59%), Positives = 21/22 (95%)
Frame = -3 / +1
Query: 631 TALALPDDGKIVAIDVTRESYD 566
TALA+P+DGKI+A+D+ +E+Y+
Sbjct: 289 TALAIPEDGKILAMDINKENYE 354
Score = 39.1 bits (79), Expect(4) = 1e-055
Identities = 18/47 (38%), Positives = 23/47 (48%)
Frame = -2 / +1
Query: 776 IRHEHRTHPMAGMAASPDXXXXXXXXXXXLGAKNAIEVGVFTGYSLL 636
+R HP M S D + AKN +E+GV+TGYSLL
Sbjct: 145 LREVTAKHPWNIMTTSADEGQFLSMLLKLINAKNTMEIGVYTGYSLL 285
Score = 36.8 bits (74), Expect(4) = 1e-055
Identities = 9/21 (42%), Positives = 16/21 (76%)
Frame = -3 / +1
Query: 241 DPRVHVCQVAIADGLXLCRRV 179
DPR+ +C + + DG+ +CRR+
Sbjct: 676 DPRIEICMLPVGDGITICRRI 738
Score = 28.6 bits (56), Expect(2) = 9e-021
Identities = 15/28 (53%), Positives = 18/28 (64%)
Frame = +3 / -2
Query: 567 S*LSRVTSMATILPSSGRARAVNEQRVP 650
S* S + S+A I PSSG ARAV + P
Sbjct: 353 S*FSLLMSIAKIFPSSGIARAVARRE*P 270
>emb|CQ760958.1| Sequence 3 from Patent WO2004002216
Length = 744
Score = 151 bits (325), Expect(4) = 1e-055
Identities = 59/88 (67%), Positives = 72/88 (81%)
Frame = -3 / +1
Query: 565 QIGSPVIEKAGVAHKIDFRVGLALPVLDQMVSEEGNKGKFDFAFVDADKVNFLNYHERLL 386
++G PVI+KAGV HKIDFR G ALPVLD+M+ +E N G +DF FVDADK N+LNYH+RL+
Sbjct: 352 ELGLPVIKKAGVDHKIDFREGPALPVLDEMIKDEKNHGSYDFIFVDADKDNYLNYHKRLI 531
Query: 385 QLLRVGGLIAYDNTLWGGSVAAPHDEPL 302
L++VGG+I YDNTLW GSV AP D PL
Sbjct: 532 DLVKVGGVIGYDNTLWNGSVVAPPDAPL 615
Score = 85.8 bits (181), Expect(2) = 9e-021
Identities = 45/78 (57%), Positives = 49/78 (62%)
Frame = +3 / -2
Query: 327 TDPPHSVLS*AMRPPTLXXXXXXXW*LRKFTLSASTNAKSNLPLFPSSETIWSSTGSASP 506
TDP H VLS + PPTL W*LR +LSAST KS LP F SS I S TG A P
Sbjct: 590 TDPFHKVLSYPITPPTLTRSINLLW*LR*LSLSASTKMKS*LP*FFSSLIISSRTGRAGP 411
Query: 507 TRKSILCATPAFSITGDP 560
+ KSIL +TPAF ITG P
Sbjct: 410 SLKSIL*STPAFLITGRP 357
Score = 40.0 bits (81), Expect(4) = 1e-055
Identities = 13/22 (59%), Positives = 21/22 (95%)
Frame = -3 / +1
Query: 631 TALALPDDGKIVAIDVTRESYD 566
TALA+P+DGKI+A+D+ +E+Y+
Sbjct: 289 TALAIPEDGKILAMDINKENYE 354
Score = 39.1 bits (79), Expect(4) = 1e-055
Identities = 18/47 (38%), Positives = 23/47 (48%)
Frame = -2 / +1
Query: 776 IRHEHRTHPMAGMAASPDXXXXXXXXXXXLGAKNAIEVGVFTGYSLL 636
+R HP M S D + AKN +E+GV+TGYSLL
Sbjct: 145 LREVTAKHPWNIMTTSADEGQFLSMLLKLINAKNTMEIGVYTGYSLL 285
Score = 36.8 bits (74), Expect(4) = 1e-055
Identities = 9/21 (42%), Positives = 16/21 (76%)
Frame = -3 / +1
Query: 241 DPRVHVCQVAIADGLXLCRRV 179
DPR+ +C + + DG+ +CRR+
Sbjct: 676 DPRIEICMLPVGDGITICRRI 738
Score = 28.6 bits (56), Expect(2) = 9e-021
Identities = 15/28 (53%), Positives = 18/28 (64%)
Frame = +3 / -2
Query: 567 S*LSRVTSMATILPSSGRARAVNEQRVP 650
S* S + S+A I PSSG ARAV + P
Sbjct: 353 S*FSLLMSIAKIFPSSGIARAVARRE*P 270
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,959,661
Number of Sequences: 7669
Number of extensions: 51004
Number of successful extensions: 749
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 213088100
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)