TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2590996.2.1
(1086 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO513961.1|CO513961 s13dSG71F0900075_156584 Glandular tr... 106 1e-056
gb|CO516956.1|CO516956 s13dSG81A0400021_468458 Glandular tr... 80 7e-024
gb|CO512186.1|CO512186 s13dSG03A0800053_113886 Glandular tr... 84 9e-023
>gb|CO513961.1|CO513961 s13dSG71F0900075_156584 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 574
Score = 106 bits (227), Expect(3) = 1e-056
Identities = 41/67 (61%), Positives = 50/67 (74%)
Frame = -2 / +3
Query: 875 AIGSNLWKEQEAPLRWLNGRAPRSVVYVNFGSITVMSNEQLVEFAWGLANTGYYFLWNVR 696
++GSN+WKE L WL + P SVVYVNFGS+TVM+ E+L+EFAWGLAN FLW +R
Sbjct: 183 SLGSNIWKEDTECLEWLGSKEPGSVVYVNFGSLTVMTEEKLLEFAWGLANCNKPFLWIIR 362
Query: 695 PDLVKSG 675
PDLV G
Sbjct: 363 PDLVTGG 383
Score = 101 bits (215), Expect(3) = 1e-056
Identities = 38/66 (57%), Positives = 44/66 (66%)
Frame = -2 / +3
Query: 680 SGDSAGLPPEFSAATEGRSMLSTWCPQAAVLEHDAVGVFLTHSGWNSTLESICGGVPMLC 501
+G S L EF R ++ +WCPQ VL H +VG FLTH GWNST ESIC GVPMLC
Sbjct: 375 TGGSKVLSSEFVNEISDRGLIVSWCPQEQVLNHPSVGGFLTHCGWNSTTESICAGVPMLC 554
Query: 500 WPFFAE 483
WPFF +
Sbjct: 555 WPFFTD 572
Score = 65.7 bits (137), Expect(2) = 1e-024
Identities = 27/66 (40%), Positives = 37/66 (56%)
Frame = +1 / -1
Query: 484 SAKKGQHSMGTPPQILSSVEFHPECVRNTPTASCSSTAACGHHVDSMLRPSVAAENSGGS 663
S K GQH++GTP +LS VEFHP+ V+N PT T +CGH + ++ NS
Sbjct: 571 SVKNGQHNIGTPAHMLSVVEFHPQ*VKNPPTEG*FKTCSCGHQLTIRPLSEISLTNSDDK 392
Query: 664 PAESPL 681
E P+
Sbjct: 391 TFEPPV 374
Score = 62.5 bits (130), Expect(2) = 1e-024
Identities = 32/65 (49%), Positives = 38/65 (58%)
Frame = +1 / -1
Query: 676 PLLTRSGRTFHRK**PVLASPHANSTSCSLDITVMLPKLTYTTERGARPLSHRSGASCSF 855
P +TRSG H+K LA P ANS S S ITV LPK T TT+ G+ SH + SF
Sbjct: 382 PPVTRSGLMIHKKGLLQLAKPQANSNSFSSVITVRLPKFT*TTDPGSLDPSHSRHSVSSF 203
Query: 856 HRLDP 870
H L+P
Sbjct: 202 HMLEP 188
Score = 45.5 bits (93), Expect(3) = 1e-056
Identities = 15/41 (36%), Positives = 28/41 (68%)
Frame = -2 / +3
Query: 1016 SAVVINTFDELDATLLAAMAKLLPPIYTVGPLQLTVRNNVQ 894
S +++NTF+EL++ ++ A++ + P +YT GPL L + Q
Sbjct: 45 STIILNTFNELESDVINALSSMFPSLYTFGPLPLLLNQTPQ 167
>gb|CO516956.1|CO516956 s13dSG81A0400021_468458 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 368
Score = 79.9 bits (168), Expect(2) = 7e-024
Identities = 25/45 (55%), Positives = 35/45 (77%)
Frame = -2 / +1
Query: 623 MLSTWCPQAAVLEHDAVGVFLTHSGWNSTLESICGGVPMLCWPFF 489
++ W PQ AVL+HD++G F+TH GWNS LE++C GVPM+ WP +
Sbjct: 232 VVRNWAPQGAVLKHDSIGGFVTHCGWNSVLEAVCEGVPMVAWPLY 366
Score = 45.5 bits (93), Expect(2) = 7e-024
Identities = 21/50 (42%), Positives = 29/50 (58%)
Frame = -2 / +1
Query: 836 LRWLNGRAPRSVVYVNFGSITVMSNEQLVEFAWGLANTGYYFLWNVRPDL 687
L WL + +SVV ++FGS+ S Q+ E A GL + FLW VR +L
Sbjct: 10 LSWLESQPSQSVVLLSFGSMGRFSKAQINEIARGLEKSEQRFLWVVRSEL 159
>gb|CO512186.1|CO512186 s13dSG03A0800053_113886 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 602
Score = 83.5 bits (176), Expect(2) = 9e-023
Identities = 33/80 (41%), Positives = 49/80 (61%)
Frame = -2 / +1
Query: 665 GLPPEFSAATEGRSMLSTWCPQAAVLEHDAVGVFLTHSGWNSTLESICGGVPMLCWPFFA 486
GLP F T R + WC Q VL H +V F+TH GWNS++E++ GVP++ +P A
Sbjct: 61 GLPDGFLVETIERGKVVKWCLQVEVLAHPSVACFMTHCGWNSSMEAVSLGVPVVTFPSRA 240
Query: 485 EQQTNCRYKCTEWGIGKEIG 426
+Q TN ++ +GIG ++G
Sbjct: 241 DQLTNAKFLADVYGIGIKMG 300
Score = 38.2 bits (77), Expect(2) = 9e-023
Identities = 18/54 (33%), Positives = 29/54 (53%)
Frame = -2 / +1
Query: 419 VQRGEVESLIREAMEGEKGQEMLRRVTELRDSAVAAAGPDGRSMRNVDRLIEEV 258
V R V+S I EA GEK +++ + + + A A G S RN+D +E++
Sbjct: 328 VTRDVVKSCILEATIGEKAEKLKQNAIKWKKKAEEAVAVGGSSHRNLDEFMEDI 489
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,512,802
Number of Sequences: 7669
Number of extensions: 110595
Number of successful extensions: 833
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 292178718
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)