TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 2542512.2.1
         (834 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|DQ222912.1|  Medicago sativa ferulate 5-hydroxylase (F5H-...    51   3e-021

>gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-K10) mRNA, complete cds
          Length = 1885

 Score = 50.6 bits (104), Expect(5) = 3e-021
 Identities = 19/38 (50%), Positives = 23/38 (60%)
 Frame = +2 / +2

                                                  
Query: 428  LPFGMGRRRCPGETLALRTAGLVLATLIQCFHWDRIDG 541
            +PFG GRR CPG  L L    L +A L+ CF W+  DG
Sbjct: 1415 IPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDG 1528




 Score = 40.0 bits (81), Expect(5) = 3e-021
 Identities = 17/33 (51%), Positives = 21/33 (63%)
 Frame = +1 / +2

                                             
Query: 160  SRLITMDDVPGLGYLQCVINETLRLYPVAPLLL 258
            SR +   D   L YL+C + ETLRL+P  PLLL
Sbjct: 1136 SRQVEEPDFEKLTYLKCALKETLRLHPPIPLLL 1234




 Score = 34.1 bits (68), Expect(5) = 3e-021
 Identities = 14/31 (45%), Positives = 19/31 (61%)
 Frame = +2 / +2

                                           
Query: 20   IMALCGXLFTAGTETTSSTIEWAMSLLLNHP 112
            I A+   +   GTET +S IEWAM+ L+  P
Sbjct: 995  IKAIIMDVMFGGTETVASAIEWAMAELMKSP 1087




 Score = 26.3 bits (51), Expect(5) = 3e-021
 Identities = 9/19 (47%), Positives = 13/19 (68%)
 Frame = +1 / +2

                               
Query: 253  LLPHESTADCTVGGYEVPR 309
            LL HE+  + TV GY +P+
Sbjct: 1226 LLLHETAEEATVNGYFIPK 1282




 Score = 25.4 bits (49), Expect(5) = 3e-021
 Identities = 12/26 (46%), Positives = 17/26 (65%)
 Frame = +2 / +2

                                      
Query: 527  DRIDGAEIDMTESGGLTMPRAVPLEA 604
            D +  +E+DM++  GLT PRA  L A
Sbjct: 1523 DGMKPSEMDMSDVFGLTAPRASRLIA 1600



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 5,529,615
Number of Sequences: 7669
Number of extensions: 55793
Number of successful extensions: 673
Number of sequences better than 1.0e-020: 1
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 217720450
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)