TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2542159.2.1
(1231 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AJ888506.1|AJ888506 AJ888506 Medicago sativa subsp. x va... 190 1e-061
gb|CO511712.1|CO511712 s13dSG01C0100006_103324 Glandular tr... 182 2e-059
gb|CO514165.1|CO514165 s13dSG70A1000069_156992 Glandular tr... 154 2e-038
>gb|AJ888506.1|AJ888506 AJ888506 Medicago sativa subsp. x varia 4-day-old Medicago sativa
subsp. x varia cDNA, mRNA sequence
Length = 742
Score = 190 bits (409), Expect(2) = 1e-061
Identities = 77/81 (95%), Positives = 78/81 (96%)
Frame = +2 / +3
Query: 149 MFLVDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQYPTSEELS 328
MFLVDWFYGVL SLGLWQKEAKILFLGLDNAGKTTLLHMLKD RLVQHQPTQYPTSEELS
Sbjct: 108 MFLVDWFYGVLXSLGLWQKEAKILFLGLDNAGKTTLLHMLKDXRLVQHQPTQYPTSEELS 287
Query: 329 IGRIKFKAFDLGGHQIARRVW 391
IG+IKFKAFDLGGHQIAR VW
Sbjct: 288 IGKIKFKAFDLGGHQIARXVW 350
Score = 130 bits (278), Expect(3) = 2e-039
Identities = 57/82 (69%), Positives = 66/82 (80%)
Frame = -2 / -1
Query: 393 FQTRRAIWWPPRSNALNLILPMLNSSDVGYCVGWCCTSRSSFSMWRRVVLPALSRPRKRI 214
FQT RAI PP+S ALNLI P+LNSSDVGY VG C T+ S S+ +VVLPALSRP+ +I
Sbjct: 352 FQTXRAI**PPKSKALNLIFPILNSSDVGY*VG*CWTNLXSLSI*SKVVLPALSRPKNKI 173
Query: 213 LASFCHSPSDASTP*NQSTRNI 148
LASFCH+PSD TP*NQST+NI
Sbjct: 172 LASFCHNPSDXRTP*NQSTKNI 107
Score = 62.0 bits (129), Expect(2) = 1e-061
Identities = 26/42 (61%), Positives = 28/42 (66%)
Frame = +2 / +1
Query: 467 KKEXXXXXXXXXXXNVPFLILGNKIDIPYAASEEELRYYLGL 592
KKE NVPFL+LGNKIDIPYAAS LRY+LGL
Sbjct: 427 KKELDALLSDESLANVPFLVLGNKIDIPYAASXNXLRYHLGL 552
Score = 60.6 bits (126), Expect(3) = 3e-025
Identities = 24/43 (55%), Positives = 33/43 (76%)
Frame = -1 / -3
Query: 277 LVLQHVEEGGLAGVVEAKEEDLSLLLPQPKRCQHPIEPVHQEH 149
LV +H+++GG AGVVE E DLSL LP KR +P++P+HQ+H
Sbjct: 236 LVFEHMKQGGFAGVVET*E*DLSLFLP*SKRX*NPVKPIHQKH 108
Score = 54.7 bits (113), Expect(3) = 3e-025
Identities = 22/49 (44%), Positives = 34/49 (69%)
Frame = -1 / -3
Query: 463 LGKTFLVNSIHQVHNSINLGVVVLPDAAGDLVAPKVERLELDPADAQLF 317
L K+FLV SI+QV + I LG+V+ PD +L+ P+++ +LD + QLF
Sbjct: 422 LSKSFLVISINQVDHCIYLGIVIFPDXTSNLMTPQIKSFKLDLPNTQLF 276
Score = 42.8 bits (87), Expect(3) = 2e-039
Identities = 17/48 (35%), Positives = 32/48 (66%)
Frame = -1 / -2
Query: 592 QAEVVPQLLL*SRVWDVNLVAQYEERNVCKGVICKKRIELLLRLGKTF 449
QA++V Q + * +W++N V++ +ER++C+ I K+ I+LL + F
Sbjct: 552 QAQMVTQXVX*GSIWNINFVSKDKERDICQ*FIRKESIQLLFLTQQIF 409
Score = 32.7 bits (65), Expect(3) = 3e-025
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = -2 / -3
Query: 561 EAAYGMSILLPSMRKGT 511
EAAYG+SIL P RKGT
Sbjct: 521 EAAYGISILFPRTRKGT 471
Score = 22.6 bits (43), Expect(3) = 2e-039
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = -2 / -1
Query: 627 KFTLPFPVVKLLRP 586
KFTLP PV K P
Sbjct: 586 KFTLPLPVGKXSSP 545
>gb|CO511712.1|CO511712 s13dSG01C0100006_103324 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 532
Score = 182 bits (393), Expect(2) = 2e-059
Identities = 73/98 (74%), Positives = 84/98 (85%)
Frame = +2 / +2
Query: 449 ERFAESKKEXXXXXXXXXXXNVPFLILGNKIDIPYAASEEELRYYLGLSNFTTGKGNVNL 628
ERFAESKKE NVPFL+LGNKIDIPYAASE+ELRY+LGLSNFTTGKG VNL
Sbjct: 137 ERFAESKKELDALLSDESLANVPFLVLGNKIDIPYAASEDELRYHLGLSNFTTGKGKVNL 316
Query: 629 ADSNVRPLEIFMCSVVRKMGYGEGFKWMSQYIK*VAAV 742
+DSNVRP+E+FMCS+V+KMGYG+GFKW+SQYIK* + V
Sbjct: 317 SDSNVRPMEVFMCSIVKKMGYGDGFKWVSQYIK*SSLV 430
Score = 146 bits (314), Expect = 4e-036
Identities = 62/136 (45%), Positives = 94/136 (69%)
Frame = -1 / -1
Query: 724 DVLRHPFEAFAIAHLAHHTAHEDLQGPDIGVGQVHVALPGCEVAQAEVVPQLLL*SRVWD 545
DVLR+PFE I H H+TAHE L G DI V Q+++ L EVAQA++V Q + * +W+
Sbjct: 412 DVLRNPFETITITHFLHNTAHEYLHGSDI*V*QIYLTLASGEVAQAQMVTQFVF*GSIWN 233
Query: 544 VNLVAQYEERNVCKGVICKKRIELLLRLGKTFLVNSIHQVHNSINLGVVVLPDAAGDLVA 365
+N V++ +ER++C+ I K+ I+LL L K+FLV SI+QV + I LG+V+ PD+ +L+
Sbjct: 232 INFVSKDKERDICQ*FIRKESIQLLF*LSKSFLVISINQVDHCIYLGIVIFPDSTSNLMT 53
Query: 364 PKVERLELDPADAQLF 317
P+++ +LD + QLF
Sbjct: 52 PQIKSFKLDLPNTQLF 5
Score = 109 bits (232), Expect(2) = 1e-031
Identities = 50/77 (64%), Positives = 55/77 (71%)
Frame = -2 / -2
Query: 741 TAATHLMY*DIHLKPSP*PILRTTLHMKISRGRTLESAKFTLPFPVVKLLRPR*YLXXXS 562
T +LMY*+ HLKPSP*PI T LHM S GRT ES KFTLP PVVKLL+P+ S
Sbjct: 429 TKELYLMY*ETHLKPSP*PIFFTILHMNTSMGRTFESDKFTLPLPVVKLLKPKW*RNSSS 250
Query: 561 EAAYGMSILLPSMRKGT 511
EAAYG+SIL P RKGT
Sbjct: 249 EAAYGISILFPRTRKGT 199
Score = 61.6 bits (128), Expect(2) = 2e-059
Identities = 24/25 (96%), Positives = 25/25 (100%)
Frame = +2 / +2
Query: 317 EELSIGRIKFKAFDLGGHQIARRVW 391
EELSIG+IKFKAFDLGGHQIARRVW
Sbjct: 5 EELSIGKIKFKAFDLGGHQIARRVW 79
Score = 42.8 bits (87), Expect(2) = 1e-031
Identities = 19/26 (73%), Positives = 21/26 (80%)
Frame = -2 / -2
Query: 393 FQTRRAIWWPPRSNALNLILPMLNSS 316
FQT RAI PP+S ALNLI P+LNSS
Sbjct: 81 FQTLRAI**PPKSKALNLIFPILNSS 4
>gb|CO514165.1|CO514165 s13dSG70A1000069_156992 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 359
Score = 154 bits (331), Expect = 2e-038
Identities = 63/69 (91%), Positives = 67/69 (97%)
Frame = +2 / +3
Query: 149 MFLVDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQYPTSEELS 328
MF+ D FYG+LASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQ+PTSEELS
Sbjct: 153 MFIFDGFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELS 332
Query: 329 IGRIKFKAF 355
IG+IKFKAF
Sbjct: 333 IGKIKFKAF 359
Score = 99.1 bits (210), Expect = 1e-021
Identities = 46/68 (67%), Positives = 51/68 (75%)
Frame = -2 / -2
Query: 351 ALNLILPMLNSSDVGYCVGWCCTSRSSFSMWRRVVLPALSRPRKRILASFCHSPSDASTP 172
ALNLI P+LNSS+VG VG C T+ SS S+ RRVV PALSRPRKRILASFC P DA P
Sbjct: 355 ALNLIFPILNSSEVGCWVG*C*TNLSSLSI*RRVVFPALSRPRKRILASFCQRPRDARMP 176
Query: 171 *NQSTRNI 148
*N S N+
Sbjct: 175 *NPSKMNM 152
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,581,499
Number of Sequences: 7669
Number of extensions: 128862
Number of successful extensions: 943
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 336281166
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)