TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 2521865.2.1
         (638 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AY639647.1|  Medicago sativa GMPase (GMP) mRNA, complete cds   262   4e-071

>gb|AY639647.1| Medicago sativa GMPase (GMP) mRNA, complete cds
          Length = 1181

 Score =  262 bits (566), Expect = 4e-071
 Identities = 104/118 (88%), Positives = 115/118 (97%)
 Frame = +2 / +3

                                                                        
Query: 2    LATGAHVVGNVLVHESAKIGEGCLIGPDVAIGPGCVVEDGVRLSRCTVMRGVRIKKHACI 181
            LA G+++VGNV+V E+AKIGEGCLIGPDVAIGPGC+VE GVRLSRCTVMRGVRIKKHACI
Sbjct: 828  LAGGSNIVGNVIVDETAKIGEGCLIGPDVAIGPGCIVESGVRLSRCTVMRGVRIKKHACI 1007

                                                                      
Query: 182  SNSIIGWHSTVGQWARIENMTILGEDVHVCDEVYSNGGVVLPHKEIKSSILKPEIVM* 355
            S+SIIGWHSTVGQWAR+ENMTILGEDVHVCDE+YSNGGVVLPHKEIK++ILKPEIVM*
Sbjct: 1008 SSSIIGWHSTVGQWARVENMTILGEDVHVCDEIYSNGGVVLPHKEIKTNILKPEIVM* 1181




 Score =  145 bits (312), Expect(2) = 1e-039
 Identities = 61/83 (73%), Positives = 67/83 (80%)
 Frame = -3 / -2

                                                                        
Query: 249  RIVIFSIRAH*PTVECQPIMLFEMQACFLIRTPRMTVQRESLTPSSTTHPGPMATSGPIR 70
            RIVIFS RAH PTVEC PI+L EMQACFLIRTPR+TVQRESLTP ST  PGP+ATSGP+R
Sbjct: 1075 RIVIFSTRAHCPTVECHPIILLEMQACFLIRTPRITVQRESLTPDSTMQPGPIATSGPMR 896

                                   
Query: 69   QPSPILALSCTSTLPTTCAPVAS 1
             PSPI A+S T T PT   P A+
Sbjct: 895  HPSPIFAVSSTITFPTMLEPPAN 827




 Score =  121 bits (258), Expect = 1e-028
 Identities = 52/113 (46%), Positives = 79/113 (69%)
 Frame = -2 / -1

                                                                        
Query: 355  SHDDLRLQNA*LDLFMWENNTAIAVHLITHMNILPQDSHILYPCPLTNS*VPADNAV*DA 176
            SHD+L LQN  L+L + +N+T IAV+LIT+MNI  +DSHIL+  PL +S +P +N   +A
Sbjct: 1181 SHDNLWLQNICLNLLVGQNHTTIAVNLITNMNIFSKDSHILHSSPLPDSGMPPNNTTGNA 1002

                                                                 
Query: 175  SMLLDTHAAHDSAAGKPHAVLHNTPRSNGDIRTNQTTFSNLGALMHQHIANNM 17
            SM LD  ++H  AA KP+  L+N  R++ DIR+N+T+ +N    +H +I +N+
Sbjct: 1001 SMFLDPDSSHYCAARKPNTRLYNAARTDSDIRSNETSLANFCCFIHNNIPHNV 843




 Score = 31.8 bits (63), Expect(2) = 1e-039
 Identities = 13/18 (72%), Positives = 15/18 (83%)
 Frame = -3 / -2

                              
Query: 354  HMTISGFRMLDLISLCGR 301
            HMTISGFR+  LISL G+
Sbjct: 1180 HMTISGFRIFVLISLWGK 1127



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 5,727,911
Number of Sequences: 7669
Number of extensions: 75965
Number of successful extensions: 1368
Number of sequences better than 1.0e-020: 1
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 157499900
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)