TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2521686.2.1
(2104 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 81 2e-042
gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 81 7e-042
gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible c... 83 7e-038
gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase ... 84 3e-035
gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase ... 84 1e-034
gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase ... 84 2e-034
gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular tr... 66 2e-027
gb|CO513716.1|CO513716 s13dSG15C0700050_140214 Glandular tr... 112 2e-025
>gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-K10) mRNA, complete cds
Length = 1885
Score = 81.3 bits (171), Expect(6) = 2e-042
Identities = 36/90 (40%), Positives = 53/90 (58%)
Frame = -2 / +2
Query: 1083 LEGDEKLGDDDMVAILWEMVFRGTDTTALLTEWCMAELVRHPAVQARVRAEVDAAVGAGG 904
L KL D++ AI+ +++F GT+T A EW MAEL++ P +V+ E+ VG
Sbjct: 962 LHNSIKLTKDNIKAIIMDVMFGGTETVASAIEWAMAELMKSPEDLKKVQQELAEVVGLSR 1141
Query: 903 CPTDADVARMPYLQAVVKETLRAHPPGPLL 814
+ D ++ YL+ +KETLR HPP PLL
Sbjct: 1142 QVEEPDFEKLTYLKCALKETLRLHPPIPLL 1231
Score = 66.1 bits (138), Expect(6) = 2e-042
Identities = 25/47 (53%), Positives = 30/47 (63%)
Frame = -2 / +2
Query: 639 DVRGVDLRLAPFGAGRRVCPGKNLGLTTVGLWVARLVHAFQWALPDG 499
D +G + PFG+GRR CPG LGL + L VA L+H F W LPDG
Sbjct: 1388 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDG 1528
Score = 52.4 bits (108), Expect(6) = 2e-042
Identities = 19/44 (43%), Positives = 25/44 (56%)
Frame = -2 / +2
Query: 795 TADVPLCNGMVVPAGTTAMVNMWAITHDAAVWADPDAFAPERFL 664
TA+ NG +P M+N WAI DA W +P++F P RFL
Sbjct: 1241 TAEEATVNGYFIPKQARVMINAWAIGRDANCWEEPESFKPSRFL 1372
Score = 28.6 bits (56), Expect(6) = 2e-042
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -2 / +2
Query: 1587 IGFAPNGEYWRRLRRVASTHLFSPRRVASHEPGR 1486
+ FA G +WR++R++ LFS + S + R
Sbjct: 455 MAFAHYGPFWRQMRKLCVMKLFSRKHAESWQSVR 556
Score = 24.0 bits (46), Expect(6) = 2e-042
Identities = 8/33 (24%), Positives = 18/33 (54%)
Frame = -2 / +2
Query: 1230 DPSNVTRRCAALVPRVQTFVRGVIDEHRRRRQN 1132
DP + R + +F+ +IDEH +++++
Sbjct: 779 DPQGLNARLVKARKDLDSFIDKIIDEHVQKKKS 877
Score = 24.0 bits (46), Expect(6) = 2e-042
Identities = 7/16 (43%), Positives = 12/16 (75%)
Frame = -1 / +2
Query: 1831 GPRGLPVFGSIFALSR 1784
GP+GLPV G++ + +
Sbjct: 215 GPKGLPVIGNMLMMDQ 262
>gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-K1) mRNA, complete cds
Length = 1904
Score = 80.8 bits (170), Expect(6) = 7e-042
Identities = 36/90 (40%), Positives = 53/90 (58%)
Frame = -2 / +1
Query: 1083 LEGDEKLGDDDMVAILWEMVFRGTDTTALLTEWCMAELVRHPAVQARVRAEVDAAVGAGG 904
L KL D++ AI+ +++F GT+T A EW MAEL++ P +V+ E+ VG
Sbjct: 976 LHNSIKLTKDNIKAIIMDVMFGGTETVASAIEWVMAELMKSPEDLKKVQQELAEVVGLSR 1155
Query: 903 CPTDADVARMPYLQAVVKETLRAHPPGPLL 814
+ D ++ YL+ +KETLR HPP PLL
Sbjct: 1156 QVEEPDFEKLTYLKCALKETLRLHPPIPLL 1245
Score = 66.1 bits (138), Expect(6) = 7e-042
Identities = 25/47 (53%), Positives = 30/47 (63%)
Frame = -2 / +1
Query: 639 DVRGVDLRLAPFGAGRRVCPGKNLGLTTVGLWVARLVHAFQWALPDG 499
D +G + PFG+GRR CPG LGL + L VA L+H F W LPDG
Sbjct: 1402 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDG 1542
Score = 51.9 bits (107), Expect(6) = 7e-042
Identities = 19/44 (43%), Positives = 25/44 (56%)
Frame = -2 / +1
Query: 795 TADVPLCNGMVVPAGTTAMVNMWAITHDAAVWADPDAFAPERFL 664
TA+ NG +P M+N WAI DA W +P++F P RFL
Sbjct: 1255 TAEEATVNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRFL 1386
Score = 28.6 bits (56), Expect(6) = 7e-042
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -2 / +1
Query: 1587 IGFAPNGEYWRRLRRVASTHLFSPRRVASHEPGR 1486
+ FA G +WR++R++ LFS + S + R
Sbjct: 469 MAFAHYGPFWRQMRKLCVMKLFSRKHAESWQSVR 570
Score = 23.5 bits (45), Expect(6) = 7e-042
Identities = 8/33 (24%), Positives = 17/33 (51%)
Frame = -2 / +1
Query: 1230 DPSNVTRRCAALVPRVQTFVRGVIDEHRRRRQN 1132
DP + R + +F+ +IDEH ++++
Sbjct: 793 DPQGLNARLVKARKDLDSFIDKIIDEHVEKKKS 891
Score = 23.5 bits (45), Expect(6) = 7e-042
Identities = 6/16 (37%), Positives = 12/16 (75%)
Frame = -1 / +1
Query: 1831 GPRGLPVFGSIFALSR 1784
GP+GLP+ G++ + +
Sbjct: 229 GPKGLPIIGNMLMMDQ 276
>gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible cinnamic acid 4-hydroxylase mRNA,
complete cds
Length = 1744
Score = 83.1 bits (175), Expect(4) = 7e-038
Identities = 37/96 (38%), Positives = 58/96 (60%)
Frame = -2 / +3
Query: 1101 VDVLLSLEGDEKLGDDDMVAILWEMVFRGTDTTALLTEWCMAELVRHPAVQARVRAEVDA 922
+D +L + ++ DD+++ I+ + +TT EW +AELV H +Q +VR E+D
Sbjct: 867 IDHILDAQKKGEINDDNVLYIVENINVAAIETTLWSIEWGIAELVNHQDIQNKVREEMDR 1046
Query: 921 AVGAGGCPTDADVARMPYLQAVVKETLRAHPPGPLL 814
+G G T+ D+ ++PYLQAV+KETLR PLL
Sbjct: 1047 VLGPGHQVTEPDLHKLPYLQAVIKETLRLRMAIPLL 1154
Score = 51.0 bits (105), Expect(4) = 7e-038
Identities = 23/61 (37%), Positives = 31/61 (50%)
Frame = -2 / +3
Query: 657 EGGADVDVRGVDLRLAPFGAGRRVCPGKNLGLTTVGLWVARLVHAFQWALPDGAAAVCLD 478
E + V+ G D R PFG GRR CPG L L +G+ + RLV + P G + +
Sbjct: 1299 EEESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNVELLPPPGQSKIDTS 1478
Query: 477 E 475
E
Sbjct: 1479 E 1481
Score = 48.7 bits (100), Expect(4) = 7e-038
Identities = 18/49 (36%), Positives = 27/49 (55%)
Frame = -2 / +3
Query: 783 PLCNGMVVPAGTTAMVNMWAITHDAAVWADPDAFAPERFLPSEGGADVD 637
P NG +PA + +VN W + ++ A W P+ F PERFL E + +
Sbjct: 1179 PKLNGFDIPAESKILVNAWWLPNNPAHWKKPEEFRPERFLEEESHVEAN 1325
Score = 38.6 bits (78), Expect(4) = 7e-038
Identities = 14/50 (28%), Positives = 28/50 (56%)
Frame = -2 / +3
Query: 1581 FAPNGEYWRRLRRVASTHLFSPRRVASHEPGRQGDAEAMLRSIAAEQSAS 1432
F GE+WR++RR+ + F+ + V + G + +AE+++ + AS
Sbjct: 384 FTVYGEHWRKMRRIMTVPFFTNKVVQQYRYGWESEAESVVNDVKNNAEAS 533
>gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase 1 (ifs1) mRNA, partial cds
Length = 1501
Score = 84.0 bits (177), Expect(3) = 3e-035
Identities = 32/85 (37%), Positives = 53/85 (62%)
Frame = -2 / +3
Query: 1068 KLGDDDMVAILWEMVFRGTDTTALLTEWCMAELVRHPAVQARVRAEVDAAVGAGGCPTDA 889
K+ + + ++ ++ GTD+TA+ TEW +AEL+ +P V + R EV + VG +
Sbjct: 822 KITKEQIKGLVVDLFSAGTDSTAVATEWALAELINNPRVLQKAREEVYSVVGKDRLVDEV 1001
Query: 888 DVARMPYLQAVVKETLRAHPPGPLL 814
D +PY++A+VKET R HPP P++
Sbjct: 1002 DTQNLPYIRAIVKETFRMHPPLPVV 1076
Score = 51.5 bits (106), Expect(3) = 3e-035
Identities = 22/68 (32%), Positives = 37/68 (54%)
Frame = -2 / +3
Query: 642 VDVRGVDLRLAPFGAGRRVCPGKNLGLTTVGLWVARLVHAFQWALPDGAAAVCLDEVLKL 463
+D+RG +L PFG+GRR+CPG NL + + +A L+ F + + + K+
Sbjct: 1248 LDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQVLGPQGQILKGDDAKV 1427
Query: 462 SLEMKTPL 439
S+E + L
Sbjct: 1428 SMEERAGL 1451
Score = 46.9 bits (96), Expect(3) = 3e-035
Identities = 16/37 (43%), Positives = 20/37 (54%)
Frame = -2 / +3
Query: 774 NGMVVPAGTTAMVNMWAITHDAAVWADPDAFAPERFL 664
NG V+P G + N+W + D W P F PERFL
Sbjct: 1107 NGYVIPEGALVLFNVWQVGRDPKYWDRPSEFRPERFL 1217
>gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase 2 (ifs2) mRNA, partial cds
Length = 1501
Score = 84.5 bits (178), Expect(3) = 1e-034
Identities = 33/85 (38%), Positives = 52/85 (61%)
Frame = -2 / +3
Query: 1068 KLGDDDMVAILWEMVFRGTDTTALLTEWCMAELVRHPAVQARVRAEVDAAVGAGGCPTDA 889
K+ D + ++ + GTD+TA+ TEW +AEL+ +P V + R EV + VG +
Sbjct: 822 KITKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEVYSVVGKDRLVDEV 1001
Query: 888 DVARMPYLQAVVKETLRAHPPGPLL 814
D +PY++A+VKET R HPP P++
Sbjct: 1002 DTQNLPYIRAIVKETFRMHPPLPVV 1076
Score = 48.7 bits (100), Expect(3) = 1e-034
Identities = 18/41 (43%), Positives = 27/41 (65%)
Frame = -2 / +3
Query: 642 VDVRGVDLRLAPFGAGRRVCPGKNLGLTTVGLWVARLVHAF 520
+D+RG +L PFG+GRR+CPG NL + + +A L+ F
Sbjct: 1248 LDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCF 1370
Score = 47.3 bits (97), Expect(3) = 1e-034
Identities = 16/37 (43%), Positives = 21/37 (56%)
Frame = -2 / +3
Query: 774 NGMVVPAGTTAMVNMWAITHDAAVWADPDAFAPERFL 664
NG V+P G + N+W + D+ W P F PERFL
Sbjct: 1107 NGYVIPEGALILFNVWQVGRDSKYWDRPSEFRPERFL 1217
>gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase 3 (ifs3) mRNA, partial cds
Length = 1501
Score = 84.5 bits (178), Expect(3) = 2e-034
Identities = 33/85 (38%), Positives = 52/85 (61%)
Frame = -2 / +3
Query: 1068 KLGDDDMVAILWEMVFRGTDTTALLTEWCMAELVRHPAVQARVRAEVDAAVGAGGCPTDA 889
K+ D + ++ + GTD+TA+ TEW +AEL+ +P V + R EV + VG +
Sbjct: 822 KITKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEVYSVVGKDRLVDEV 1001
Query: 888 DVARMPYLQAVVKETLRAHPPGPLL 814
D +PY++A+VKET R HPP P++
Sbjct: 1002 DTQNLPYIRAIVKETFRMHPPLPVV 1076
Score = 48.7 bits (100), Expect(3) = 2e-034
Identities = 18/41 (43%), Positives = 27/41 (65%)
Frame = -2 / +3
Query: 642 VDVRGVDLRLAPFGAGRRVCPGKNLGLTTVGLWVARLVHAF 520
+D+RG +L PFG+GRR+CPG NL + + +A L+ F
Sbjct: 1248 LDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCF 1370
Score = 46.4 bits (95), Expect(3) = 2e-034
Identities = 16/37 (43%), Positives = 20/37 (54%)
Frame = -2 / +3
Query: 774 NGMVVPAGTTAMVNMWAITHDAAVWADPDAFAPERFL 664
NG V+P G + N+W + D W P F PERFL
Sbjct: 1107 NGYVIPEGALILFNVWQVGRDPKYWDRPSEFRPERFL 1217
>gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 66.1 bits (138), Expect(3) = 2e-027
Identities = 25/47 (53%), Positives = 30/47 (63%)
Frame = -2 / +2
Query: 639 DVRGVDLRLAPFGAGRRVCPGKNLGLTTVGLWVARLVHAFQWALPDG 499
D +G + PFG+GRR CPG LGL + L VA L+H F W LPDG
Sbjct: 248 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDG 388
Score = 51.9 bits (107), Expect(3) = 2e-027
Identities = 19/44 (43%), Positives = 25/44 (56%)
Frame = -2 / +2
Query: 795 TADVPLCNGMVVPAGTTAMVNMWAITHDAAVWADPDAFAPERFL 664
TA+ NG +P M+N WAI DA W +P++F P RFL
Sbjct: 101 TAEEATVNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRFL 232
Score = 38.2 bits (77), Expect(3) = 2e-027
Identities = 14/27 (51%), Positives = 19/27 (70%)
Frame = -2 / +2
Query: 894 DADVARMPYLQAVVKETLRAHPPGPLL 814
+ D ++ YL+ +KETLR HPP PLL
Sbjct: 11 EPDFEKLTYLKCALKETLRLHPPIPLL 91
>gb|CO513716.1|CO513716 s13dSG15C0700050_140214 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 300
Score = 112 bits (239), Expect = 2e-025
Identities = 46/91 (50%), Positives = 57/91 (62%)
Frame = -2 / +2
Query: 762 VPAGTTAMVNMWAITHDAAVWADPDAFAPERFLPSEGGADVDVRGVDLRLAPFGAGRRVC 583
+P G+T +VN+WAI D WADP F PERFLP DVDV+G D + PFGAGRR+C
Sbjct: 5 IPKGSTLLVNVWAIARDPGKWADPLMFNPERFLPGGENCDVDVKGNDFEVIPFGAGRRIC 184
Query: 582 PGKNLGLTTVGLWVARLVHAFQWALPDGAAA 490
G NL + V L +A LVH+ L +G A
Sbjct: 185 AGLNLRIRMVQLQIATLVHSLNRELENGLNA 277
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 14,023,993
Number of Sequences: 7669
Number of extensions: 179607
Number of successful extensions: 1818
Number of sequences better than 1.0e-020: 8
length of database: 1,248,568
effective HSP length: 45
effective length of database: 903,463
effective search space used: 591768265
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)