TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2521589.2.1
(1689 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible c... 421 0.0
gb|CO515318.1|CO515318 s13dSG48E0800067_417695 Glandular tr... 363 e-101
gb|CO513037.1|CO513037 s13dSG09H0900076_122000 Glandular tr... 202 4e-077
gb|CO514181.1|CO514181 s13dSG70C1200086_157024 Glandular tr... 199 1e-051
gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 83 1e-048
gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 82 4e-048
gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase ... 79 1e-028
gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase ... 77 9e-028
gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular tr... 119 1e-027
gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase ... 79 2e-027
gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular tr... 54 1e-021
>gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible cinnamic acid 4-hydroxylase mRNA,
complete cds
Length = 1744
Score = 421 bits (914), Expect(2) = 0.0
Identities = 173/219 (78%), Positives = 194/219 (88%)
Frame = -3 / +3
Query: 1549 WLQVGDDLNHRNLFVMAKRFGDIFLLRMGVRNLVVVSTPELAKEVLHTQGVEFGSRTRNV 1370
WLQVGDDLNHRNL AKRFG+IFLLRMG RNLVVVS+PELAKEVLHTQ VEFGSRTRNV
Sbjct: 165 WLQVGDDLNHRNLTDYAKRFGEIFLLRMGQRNLVVVSSPELAKEVLHTQCVEFGSRTRNV 344
Query: 1369 VFDIFTGKGQDMVFTVYGDHWRKMRRIMTVPFFTNKVVAQNRGGWEEEARLVVEDVRKDP 1190
VFDIFTGKGQDMVFTVYG+HWRKMRRIMTVPFFTNKVV Q R GWE EA VV DV+ +
Sbjct: 345 VFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRYGWESEAESVVNDVKNNA 524
Query: 1189 EAAAGGVVLRRRLQLMMYNDMFRIMFDRRFDSEHDPLFNKLKALNAERSRLSQSFEYNYG 1010
EA+ GG+V+R+RLQLMMYN M+RIMFDRRF+SE DPLF KLKALN ERSRL+QSFEYNYG
Sbjct: 525 EASVGGIVIRKRLQLMMYNIMYRIMFDRRFESEEDPLFVKLKALNGERSRLAQSFEYNYG 704
Query: 1009 DFIPVLRPFLRGYLNRCHDLKTRRMKVFEDNFVQERKKV 893
DFIP+LRPFL+GYL C ++K RR+++F+D FV ERKK+
Sbjct: 705 DFIPILRPFLKGYLKVCKEVKDRRLQLFKDYFVDERKKL 821
Score = 403 bits (874), Expect(2) = 0.0
Identities = 168/239 (70%), Positives = 192/239 (80%)
Frame = -3 / +3
Query: 907 ERKKVMAQTGEIRCAMDHILEAERKGEINHDNVLYIVENINVAAIETTLWSIEWGIAELV 728
E K ++CA+DHIL+A++KGEIN DNVLYIVENINVAAIETTLWSIEWGIAELV
Sbjct: 822 ESTKSTTSNDGLKCAIDHILDAQKKGEINDDNVLYIVENINVAAIETTLWSIEWGIAELV 1001
Query: 727 NHPAIQHKLREELASVLGAGVPVTEPDLERLPYLQAIVKETLRLRMAIPLLVPHMNLNDG 548
NH IQ+K+REE+ VLG G VTEPDL +LPYLQA++KETLRLRMAIPLLVPHMNL+D
Sbjct: 1002 NHQDIQNKVREEMDRVLGPGHQVTEPDLHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDP 1181
Query: 547 KLAGYDIPAESKILVNAWFLANDPKRWVRPDEFRPERFLEEEKSVEAHGNDFRFVPFGVG 368
KL G+DIPAESKILVNAW+L N+P W +P+EFRPERFLEEE VEA+GNDFR++PFGVG
Sbjct: 1182 KLNGFDIPAESKILVNAWWLPNNPAHWKKPEEFRPERFLEEESHVEANGNDFRYLPFGVG 1361
Query: 367 RRSCPXXXXXXXXXXXXXGRLVQNFQLLPPPGLDKIDTTEKPGQFSNQIAKHATIVCKP 191
RRSCP GRLVQN +LLPPPG KIDT+EK GQFS I KH+TIV KP
Sbjct: 1362 RRSCPGIILALPILGITIGRLVQNVELLPPPGQSKIDTSEKGGQFSLHILKHSTIVAKP 1538
Score = 181 bits (390), Expect(4) = e-112
Identities = 79/106 (74%), Positives = 88/106 (83%)
Frame = +3 / -1
Query: 1236 HPPRFWATTLLVKKGTVMMRRILRQWSPYTVNTMSCPLPVKMSNTTLRVREPNSTPCVWS 1415
HP R+ TTL VKKGTVM+ RILRQ SP TVNTMSCPLPVK+SNTTLRVR+PNST CVW
Sbjct: 478 HP*RYC*TTLFVKKGTVMILRILRQCSP*TVNTMSCPLPVKISNTTLRVRDPNSTHCVWR 299
Query: 1416 TSLASSGVDTTTRLRTPMRSRKMSPNRFAMTNRLRWFRSSPTCSQF 1553
TSLASSG +TTTRLR P+R RK+SP R A + RLRW RSSPT SQ+
Sbjct: 298 TSLASSGEETTTRLRWPIRRRKISPKRLA*SVRLRWLRSSPT*SQY 161
Score = 164 bits (353), Expect(4) = e-112
Identities = 86/150 (57%), Positives = 99/150 (66%)
Frame = +3 / -1
Query: 477 GSLARNQALTRILDSAGMS*PASLPSLRFMWGTSSGMAMRRRSVSLTMAXXXXXXXXXXX 656
G + ALT IL SAG+S P S S RFM GT SG+A+RRRSVSL A
Sbjct: 1252 GLFGSHHALTNILLSAGISKPLSFGS*RFMCGTRSGIAIRRRSVSLITACR*GSLWRSGS 1073
Query: 657 VTGTPAPSTEASSSRSLCWMAGWFTSSAMPHSIDHSVVSIAATLMFSTM*RTLSWLISPF 836
VT P P T + SS +L WM+ WFTSSA+PHSIDHSVVSIAATLMFSTM*RTLS LISPF
Sbjct: 1072 VTWCPGPRTLSISSLTLFWMSWWFTSSAIPHSIDHSVVSIAATLMFSTM*RTLSSLISPF 893
Query: 837 LSASRM*SMAHRISPV*AITFLRSCTKLSS 926
AS+M*S+AH + + + S LSS
Sbjct: 892 F*ASKM*SIAHFSPSLLVVLLVLSSFFLSS 803
Score = 102 bits (217), Expect(4) = e-112
Identities = 48/75 (64%), Positives = 50/75 (66%)
Frame = +3 / -1
Query: 192 GLQTMVACLAIWLLNWPGFSVVSILSSPGGGSSWKFCTSRPRVMPMIGSARMIPGQLRRP 371
GL TMV CL+I LN P FS VSI PGGG S F T RP V+P IG ARMIPGQL P
Sbjct: 1537 GLATMVECLSICRLNCPPFSEVSIFDCPGGGKSSTF*TRRPIVIPRIGKARMIPGQLLLP 1358
Query: 372 TPKGTKRKSLPWAST 416
TPKG KS P AST
Sbjct: 1357 TPKGRYLKSFPLAST 1313
Score = 65.2 bits (136), Expect(3) = 1e-026
Identities = 36/100 (36%), Positives = 56/100 (56%)
Frame = +2 / -3
Query: 467 PPLGVVGEEPGIDEDLGLGGDVVAGELAVVEVHVGDQQRDGHAQAERLLDDGLKVGEALE 646
P VV + P ID+ L LG D+ EL +++VHV D++ D H+ + L D L V +E
Sbjct: 1262 PVSRVVWKPPCIDQYLALGWDIKTIELWIMKVHVWDEEWDRHS*TKCLFDHSL*VR*LVE 1083
Query: 647 VRLRHRHAGAQHRGELLPELVLDGRVVHQLGDAPLDRPQC 766
+ L + + +++ LP VLD VVH L ++P + P C
Sbjct: 1082 IWLSYLVSWSKNSVHFLPYFVLDVLVVH*LSNSPFN*P*C 963
Score = 52.4 bits (108), Expect(3) = 1e-026
Identities = 22/50 (44%), Positives = 31/50 (62%)
Frame = +2 / -3
Query: 1265 VGEEGDGHDAPHLAPVVAVHREHHVLPLAREDVEHHVAGAGAELHALRVE 1414
V EE +GHD+ H P+ AV EHHVL E++E++V +E + L VE
Sbjct: 449 VCEERNGHDSAHFTPMFAVDSEHHVLSFTCENIEYYVTCT*SEFNTLCVE 300
Score = 43.7 bits (89), Expect(3) = 1e-026
Identities = 23/45 (51%), Positives = 28/45 (62%)
Frame = +3 / -1
Query: 894 TFLRSCTKLSSKTFMRRVLRSWQRLR*PRRKGRSTGMKSP*LYSK 1028
+F S TK S K+ R L S Q +*P +KG G+KSP*LYSK
Sbjct: 820 SFFLSSTK*SLKSCNLRSLTSLQTFK*PFKKGLKIGIKSP*LYSK 686
Score = 22.6 bits (43), Expect(4) = e-112
Identities = 14/48 (29%), Positives = 20/48 (41%)
Frame = +2 / -3
Query: 1541 LQPVSHHGGARGPRWEAVPLAGQLGHGDGHHSRGEQAQQGLL*EGEVH 1684
L+PVS WE A + DG E+ +GL E ++H
Sbjct: 173 LKPVSEDRDWDWTWWEFEAFATEF*DCDGDCGGNEEG*KGLFKEEKIH 30
>gb|CO515318.1|CO515318 s13dSG48E0800067_417695 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 557
Score = 363 bits (787), Expect = e-101
Identities = 151/184 (82%), Positives = 164/184 (89%)
Frame = -3 / +1
Query: 1543 QVGDDLNHRNLFVMAKRFGDIFLLRMGVRNLVVVSTPELAKEVLHTQGVEFGSRTRNVVF 1364
QVGDDLNHRNL AKRFG+IFLLRMG RNLVVVS+PELAKEVLHTQGVEFGSRTRNVVF
Sbjct: 4 QVGDDLNHRNLTDYAKRFGEIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVF 183
Query: 1363 DIFTGKGQDMVFTVYGDHWRKMRRIMTVPFFTNKVVAQNRGGWEEEARLVVEDVRKDPEA 1184
DIFT KGQDMVFTVYG+HWRKMRRIMTVPFFTNKVV Q R GWE EA VV DV+ + EA
Sbjct: 184 DIFTSKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRYGWESEAESVVNDVKNNAEA 363
Query: 1183 AAGGVVLRRRLQLMMYNDMFRIMFDRRFDSEHDPLFNKLKALNAERSRLSQSFEYNYGDF 1004
+ GG+V+R+RLQLMMYN M+RIMFDRRF+SE DPLF KLKALN ERSRL+ SFEYNYGDF
Sbjct: 364 SVGGIVIRKRLQLMMYNIMYRIMFDRRFESEEDPLFVKLKALNGERSRLAXSFEYNYGDF 543
Query: 1003 IPVL 992
IP+L
Sbjct: 544 IPIL 555
Score = 177 bits (381), Expect = 4e-045
Identities = 77/103 (74%), Positives = 84/103 (81%)
Frame = +3 / -1
Query: 1236 HPPRFWATTLLVKKGTVMMRRILRQWSPYTVNTMSCPLPVKMSNTTLRVREPNSTPCVWS 1415
HP R+ TTL VK GTVM+ RILRQ SP TVNTMSCPL VKMS TTLRVR+PNSTPCVW
Sbjct: 311 HP*RYC*TTLFVKNGTVMILRILRQCSP*TVNTMSCPLLVKMSKTTLRVRDPNSTPCVWR 132
Query: 1416 TSLASSGVDTTTRLRTPMRSRKMSPNRFAMTNRLRWFRSSPTC 1544
TSLASSG +TTTRLR P+R RK+SP R A + RLRW RSSPTC
Sbjct: 131 TSLASSGEETTTRLR*PIRRRKISPKRLA*SVRLRWLRSSPTC 3
>gb|CO513037.1|CO513037 s13dSG09H0900076_122000 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 526
Score = 202 bits (435), Expect(2) = 4e-077
Identities = 80/116 (68%), Positives = 98/116 (84%)
Frame = -3 / +3
Query: 1240 GWEEEARLVVEDVRKDPEAAAGGVVLRRRLQLMMYNDMFRIMFDRRFDSEHDPLFNKLKA 1061
GWE EA VV DV+ + EA+ GG+V+ +RLQLMMYN M+RIMFDRRF+SE DPLF KLKA
Sbjct: 6 GWESEAESVVNDVKNNAEASVGGIVIWKRLQLMMYNIMYRIMFDRRFESEEDPLFVKLKA 185
Query: 1060 LNAERSRLSQSFEYNYGDFIPVLRPFLRGYLNRCHDLKTRRMKVFEDNFVQERKKV 893
LN ERSRL+QSFEYNYGDFIP+LRPFL+GYL C ++K RR+++F+D FV ERKK+
Sbjct: 186 LNGERSRLAQSFEYNYGDFIPILRPFLKGYLKVCKEVKDRRLQLFKDYFVDERKKL 353
Score = 102 bits (217), Expect(2) = 4e-077
Identities = 41/57 (71%), Positives = 47/57 (82%)
Frame = -3 / +3
Query: 907 ERKKVMAQTGEIRCAMDHILEAERKGEINHDNVLYIVENINVAAIETTLWSIEWGIA 737
E K ++CA+DHIL+A++KGEIN DNVLYIVENINVAAIETTLWSIEWGIA
Sbjct: 354 ESTKSTTSNDGLKCAIDHILDAQKKGEINDDNVLYIVENINVAAIETTLWSIEWGIA 524
>gb|CO514181.1|CO514181 s13dSG70C1200086_157024 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 358
Score = 199 bits (428), Expect = 1e-051
Identities = 83/111 (74%), Positives = 97/111 (87%)
Frame = -3 / +1
Query: 874 IRCAMDHILEAERKGEINHDNVLYIVENINVAAIETTLWSIEWGIAELVNHPAIQHKLRE 695
++CA+DHIL+A++KGEIN +VLYI ENIN AAIETTLW+IEWGIAELVNH IQ+K+RE
Sbjct: 25 LKCAIDHILDAQKKGEINGGDVLYIGENINGAAIETTLWAIEWGIAELVNHQDIQNKVRE 204
Query: 694 ELASVLGAGVPVTEPDLERLPYLQAIVKETLRLRMAIPLLVPHMNLNDGKL 542
E+ VLG G VTEPDL +LPYLQA +KETLRLRMAIPLLVPHMNL+D KL
Sbjct: 205 EMDRVLGPGHQVTEPDLHKLPYLQAGIKETLRLRMAIPLLVPHMNLHDAKL 357
Score = 130 bits (278), Expect = 6e-031
Identities = 66/109 (60%), Positives = 74/109 (67%)
Frame = +3 / -3
Query: 543 SLPSLRFMWGTSSGMAMRRRSVSLTMAXXXXXXXXXXXVTGTPAPSTEASSSRSLCWMAG 722
S S RFM GT SG+A+RRRSVSL A VT P P T + SS +L WM+
Sbjct: 356 SFAS*RFMCGTRSGIAIRRRSVSLIPACR*GSLWRSGSVTWCPGPRTLSISSLTLFWMSW 177
Query: 723 WFTSSAMPHSIDHSVVSIAATLMFSTM*RTLSWLISPFLSASRM*SMAH 869
WFTSSA+PHSI HSVVSIAA LMFS M*RT LISPF AS+M*S+AH
Sbjct: 176 WFTSSAIPHSIAHSVVSIAAPLMFSPM*RTSPPLISPFF*ASKM*SIAH 30
>gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-K10) mRNA, complete cds
Length = 1885
Score = 83.1 bits (175), Expect(4) = 1e-048
Identities = 36/86 (41%), Positives = 53/86 (61%)
Frame = -3 / +2
Query: 829 EINHDNVLYIVENINVAAIETTLWSIEWGIAELVNHPAIQHKLREELASVLGAGVPVTEP 650
++ DN+ I+ ++ ET +IEW +AEL+ P K+++ELA V+G V EP
Sbjct: 977 KLTKDNIKAIIMDVMFGGTETVASAIEWAMAELMKSPEDLKKVQQELAEVVGLSRQVEEP 1156
Query: 649 DLERLPYLQAIVKETLRLRMAIPLLV 572
D E+L YL+ +KETLRL IPLL+
Sbjct: 1157 DFEKLTYLKCALKETLRLHPPIPLLL 1234
Score = 81.3 bits (171), Expect(4) = 1e-048
Identities = 35/91 (38%), Positives = 50/91 (54%)
Frame = -3 / +2
Query: 1534 DDLNHRNLFVMAKRFGDIFLLRMGVRNLVVVSTPELAKEVLHTQGVEFGSRTRNVVFDIF 1355
D L HR L +AK++G IF LRMG ++V +S + A++VL Q F +R V
Sbjct: 257 DQLTHRGLANLAKKYGGIFHLRMGFLHMVAISDADAARQVLQVQDNIFSNRPATVAIKYL 436
Query: 1354 TGKGQDMVFTVYGDHWRKMRRIMTVPFFTNK 1262
T DM F YG WR+MR++ + F+ K
Sbjct: 437 TYDRADMAFAHYGPFWRQMRKLCVMKLFSRK 529
Score = 54.2 bits (112), Expect(4) = 1e-048
Identities = 17/50 (34%), Positives = 30/50 (60%)
Frame = -3 / +2
Query: 577 LVPHMNLNDGKLAGYDIPAESKILVNAWFLANDPKRWVRPDEFRPERFLE 428
L+ H + + GY IP ++++++NAW + D W P+ F+P RFL+
Sbjct: 1226 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWEEPESFKPSRFLK 1375
Score = 38.6 bits (78), Expect(4) = 1e-048
Identities = 16/45 (35%), Positives = 21/45 (46%)
Frame = -3 / +2
Query: 403 GNDFRFVPFGVGRRSCPXXXXXXXXXXXXXGRLVQNFQLLPPPGL 269
G++F F+PFG GRRSCP L+ F P G+
Sbjct: 1397 GSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGM 1531
>gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-K1) mRNA, complete cds
Length = 1904
Score = 81.7 bits (172), Expect(4) = 4e-048
Identities = 36/86 (41%), Positives = 53/86 (61%)
Frame = -3 / +1
Query: 829 EINHDNVLYIVENINVAAIETTLWSIEWGIAELVNHPAIQHKLREELASVLGAGVPVTEP 650
++ DN+ I+ ++ ET +IEW +AEL+ P K+++ELA V+G V EP
Sbjct: 991 KLTKDNIKAIIMDVMFGGTETVASAIEWVMAELMKSPEDLKKVQQELAEVVGLSRQVEEP 1170
Query: 649 DLERLPYLQAIVKETLRLRMAIPLLV 572
D E+L YL+ +KETLRL IPLL+
Sbjct: 1171 DFEKLTYLKCALKETLRLHPPIPLLL 1248
Score = 81.3 bits (171), Expect(4) = 4e-048
Identities = 35/91 (38%), Positives = 50/91 (54%)
Frame = -3 / +1
Query: 1534 DDLNHRNLFVMAKRFGDIFLLRMGVRNLVVVSTPELAKEVLHTQGVEFGSRTRNVVFDIF 1355
D L HR L +AK++G IF LRMG ++V +S + A++VL Q F +R V
Sbjct: 271 DQLTHRGLANLAKKYGGIFHLRMGFLHMVAISDADAARQVLQVQDNIFSNRPATVAIKYL 450
Query: 1354 TGKGQDMVFTVYGDHWRKMRRIMTVPFFTNK 1262
T DM F YG WR+MR++ + F+ K
Sbjct: 451 TYDRADMAFAHYGPFWRQMRKLCVMKLFSRK 543
Score = 53.8 bits (111), Expect(4) = 4e-048
Identities = 17/50 (34%), Positives = 30/50 (60%)
Frame = -3 / +1
Query: 577 LVPHMNLNDGKLAGYDIPAESKILVNAWFLANDPKRWVRPDEFRPERFLE 428
L+ H + + GY IP ++++++NAW + D W P+ F+P RFL+
Sbjct: 1240 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRFLK 1389
Score = 38.6 bits (78), Expect(4) = 4e-048
Identities = 16/45 (35%), Positives = 21/45 (46%)
Frame = -3 / +1
Query: 403 GNDFRFVPFGVGRRSCPXXXXXXXXXXXXXGRLVQNFQLLPPPGL 269
G++F F+PFG GRRSCP L+ F P G+
Sbjct: 1411 GSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGM 1545
>gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase 3 (ifs3) mRNA, partial cds
Length = 1501
Score = 78.5 bits (165), Expect(2) = 1e-028
Identities = 33/85 (38%), Positives = 54/85 (63%)
Frame = -3 / +3
Query: 829 EINHDNVLYIVENINVAAIETTLWSIEWGIAELVNHPAIQHKLREELASVLGAGVPVTEP 650
+I D++ +V + A ++T + EW +AEL+N+P + K REE+ SV+G V E
Sbjct: 822 KITKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEVYSVVGKDRLVDEV 1001
Query: 649 DLERLPYLQAIVKETLRLRMAIPLL 575
D + LPY++AIVKET R+ +P++
Sbjct: 1002 DTQNLPYIRAIVKETFRMHPPLPVV 1076
Score = 63.4 bits (132), Expect(2) = 1e-028
Identities = 27/53 (50%), Positives = 31/53 (58%)
Frame = -3 / +3
Query: 547 KLAGYDIPAESKILVNAWFLANDPKRWVRPDEFRPERFLEEEKSVEAHGNDFR 389
++ GY IP + IL N W + DPK W RP EFRPERFLE EA D R
Sbjct: 1101 EINGYVIPEGALILFNVWQVGRDPKYWDRPSEFRPERFLETGAEGEARPLDLR 1259
>gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase 1 (ifs1) mRNA, partial cds
Length = 1501
Score = 77.1 bits (162), Expect(2) = 9e-028
Identities = 32/85 (37%), Positives = 54/85 (63%)
Frame = -3 / +3
Query: 829 EINHDNVLYIVENINVAAIETTLWSIEWGIAELVNHPAIQHKLREELASVLGAGVPVTEP 650
+I + + +V ++ A ++T + EW +AEL+N+P + K REE+ SV+G V E
Sbjct: 822 KITKEQIKGLVVDLFSAGTDSTAVATEWALAELINNPRVLQKAREEVYSVVGKDRLVDEV 1001
Query: 649 DLERLPYLQAIVKETLRLRMAIPLL 575
D + LPY++AIVKET R+ +P++
Sbjct: 1002 DTQNLPYIRAIVKETFRMHPPLPVV 1076
Score = 62.0 bits (129), Expect(2) = 9e-028
Identities = 26/53 (49%), Positives = 31/53 (58%)
Frame = -3 / +3
Query: 547 KLAGYDIPAESKILVNAWFLANDPKRWVRPDEFRPERFLEEEKSVEAHGNDFR 389
++ GY IP + +L N W + DPK W RP EFRPERFLE EA D R
Sbjct: 1101 EINGYVIPEGALVLFNVWQVGRDPKYWDRPSEFRPERFLETGAEGEAGPLDLR 1259
>gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 506
Score = 119 bits (254), Expect = 1e-027
Identities = 44/123 (35%), Positives = 81/123 (65%)
Frame = -3 / +2
Query: 799 VENINVAAIETTLWSIEWGIAELVNHPAIQHKLREELASVLGAGVPVTEPDLERLPYLQA 620
++++ V E++ + W ++E+V +P + K + E+ V V E +L +L YL++
Sbjct: 83 LQDMFVGGTESSSEVVLWAMSEMVKNPMVMEKAQVEVRRVFDKKGYVDETELHQLIYLKS 262
Query: 619 IVKETLRLRMAIPLLVPHMNLNDGKLAGYDIPAESKILVNAWFLANDPKRWVRPDEFRPE 440
++KET+RL ++PLL+P ++ ++ GYDIPA+++++VNAW + DP+ WV + F+PE
Sbjct: 263 VIKETMRLHPSLPLLIPRESIKPCQINGYDIPAKTRVIVNAWAIGRDPRYWVDAESFKPE 442
Query: 439 RFL 431
RFL
Sbjct: 443 RFL 451
>gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase 2 (ifs2) mRNA, partial cds
Length = 1501
Score = 78.5 bits (165), Expect(2) = 2e-027
Identities = 33/85 (38%), Positives = 54/85 (63%)
Frame = -3 / +3
Query: 829 EINHDNVLYIVENINVAAIETTLWSIEWGIAELVNHPAIQHKLREELASVLGAGVPVTEP 650
+I D++ +V + A ++T + EW +AEL+N+P + K REE+ SV+G V E
Sbjct: 822 KITKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEVYSVVGKDRLVDEV 1001
Query: 649 DLERLPYLQAIVKETLRLRMAIPLL 575
D + LPY++AIVKET R+ +P++
Sbjct: 1002 DTQNLPYIRAIVKETFRMHPPLPVV 1076
Score = 59.7 bits (124), Expect(2) = 2e-027
Identities = 26/53 (49%), Positives = 30/53 (56%)
Frame = -3 / +3
Query: 547 KLAGYDIPAESKILVNAWFLANDPKRWVRPDEFRPERFLEEEKSVEAHGNDFR 389
++ GY IP + IL N W + D K W RP EFRPERFLE EA D R
Sbjct: 1101 EINGYVIPEGALILFNVWQVGRDSKYWDRPSEFRPERFLETGAEGEARPLDLR 1259
>gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 53.8 bits (111), Expect(3) = 1e-021
Identities = 17/50 (34%), Positives = 30/50 (60%)
Frame = -3 / +2
Query: 577 LVPHMNLNDGKLAGYDIPAESKILVNAWFLANDPKRWVRPDEFRPERFLE 428
L+ H + + GY IP ++++++NAW + D W P+ F+P RFL+
Sbjct: 86 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRFLK 235
Score = 43.2 bits (88), Expect(3) = 1e-021
Identities = 18/30 (60%), Positives = 22/30 (73%)
Frame = -3 / +2
Query: 661 VTEPDLERLPYLQAIVKETLRLRMAIPLLV 572
V EPD E+L YL+ +KETLRL IPLL+
Sbjct: 5 VEEPDFEKLTYLKCALKETLRLHPPIPLLL 94
Score = 38.6 bits (78), Expect(3) = 1e-021
Identities = 16/45 (35%), Positives = 21/45 (46%)
Frame = -3 / +2
Query: 403 GNDFRFVPFGVGRRSCPXXXXXXXXXXXXXGRLVQNFQLLPPPGL 269
G++F F+PFG GRRSCP L+ F P G+
Sbjct: 257 GSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGM 391
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,339,488
Number of Sequences: 7669
Number of extensions: 115109
Number of successful extensions: 2160
Number of sequences better than 1.0e-020: 12
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 471966376
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)