TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2521502.2.2
(1862 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO515609.1|CO515609 s13dSG47E0500039_418277 Glandular tr... 248 3e-091
gb|CO511782.1|CO511782 s13dSG02A0400033_103464 Glandular tr... 165 4e-082
gb|CO515300.1|CO515300 s13dSG48C1200098_417659 Glandular tr... 127 1e-075
gb|CO514893.1|CO514893 s13dSG56F0300031_328446 Glandular tr... 159 4e-063
gb|CO512362.1|CO512362 s13dSG80A0800053_114238 Glandular tr... 105 3e-037
emb|AJ245868.1|MSA245868 Medicago sativa mRNA for cysteine ... 64 4e-032
>gb|CO515609.1|CO515609 s13dSG47E0500039_418277 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 591
Score = 248 bits (536), Expect(3) = 3e-091
Identities = 93/125 (74%), Positives = 107/125 (85%)
Frame = -1 / +1
Query: 1133 NNGGIDTEKDYPYKGTDGRCDVNTKNAKVVTIDSYEDIPANDEKSLQKAVANQPVSVAIE 954
+NGGID+E+DYPYK DGRCD N KNAKVVTID YED+PA DE +LQKAVANQP++VA+E
Sbjct: 4 SNGGIDSEEDYPYKAIDGRCDQNRKNAKVVTIDDYEDVPAYDELALQKAVANQPIAVAVE 183
Query: 953 AAGTAFQLYSSGIFTGSCGTALDHGVTAVGYGTENGKDYWIVKNSWGSSWGESGYVRMER 774
G FQLY G+FTG CGTALDHGV AVGYGTENGKDYWIV+NSWG SWGE GY+R+ER
Sbjct: 184 GGGREFQLYEYGVFTGRCGTALDHGVAAVGYGTENGKDYWIVRNSWGGSWGEQGYIRLER 363
Query: 773 NIKAS 759
N+ +S
Sbjct: 364 NLASS 378
Score = 171 bits (367), Expect(3) = 2e-048
Identities = 81/121 (66%), Positives = 88/121 (72%)
Frame = +1 / -2
Query: 772 LRSILTYPDSPQLLPHEFFTIQ*SLPFSVP*PTAVTPWSSAVPQLPVKIPEL*S*NAVPA 951
LRS L P SPQL PHEF TIQ*S PFSVP PTA TPWSSAVP LPV P + N++P
Sbjct: 365 LRSSLM*PCSPQLPPHEFLTIQ*SFPFSVPYPTAATPWSSAVPHLPVNTPYSYNWNSLPP 186
Query: 952 ASIATLTGWFATAFCRLFSSFAGISS*LSIVTTFAFFVLTSHRPSVPL*G*SFSVSIPPL 1131
S AT GWFATAFC+ SS AG SS* SIVTT AFF+ SH PS+ L*G*S S S+PPL
Sbjct: 185 PSTATAIGWFATAFCKANSS*AGTSS*SSIVTTLAFFLFWSHLPSIAL*G*SSSESMPPL 6
Query: 1132 L 1134
L
Sbjct: 5 L 3
Score = 85.4 bits (180), Expect(3) = 3e-091
Identities = 26/36 (72%), Positives = 31/36 (86%)
Frame = -1 / +1
Query: 656 AVCDNYYSCPDSTTCCCIYEYGKYCFAWGCCPLEGA 549
+VCD+YYSC + +TCCCIYEYG+ CF WGCCPLE A
Sbjct: 484 SVCDSYYSCAEGSTCCCIYEYGRSCFEWGCCPLESA 591
Score = 37.3 bits (75), Expect(3) = 3e-091
Identities = 12/15 (80%), Positives = 15/15 (100%)
Frame = -1 / +1
Query: 758 SGKCGIAVEPSYPLK 714
+GKCGIA+EPSYP+K
Sbjct: 382 AGKCGIAIEPSYPIK 426
Score = 30.9 bits (61), Expect(3) = 2e-048
Identities = 17/25 (68%), Positives = 18/25 (72%)
Frame = +1 / -2
Query: 580 KQYLPYS*MQQQVVLSGHE**LSHT 654
KQ LPYS*+QQQV S **LS T
Sbjct: 560 KQDLPYS*IQQQVEPSAQL**LSQT 486
Score = 25.4 bits (49), Expect(3) = 2e-048
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = +1 / -2
Query: 709 PSFNGYDGSTAIPHLP 756
P G DGS AIPH P
Sbjct: 431 PFLIG*DGSIAIPHFP 384
>gb|CO511782.1|CO511782 s13dSG02A0400033_103464 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 536
Score = 165 bits (355), Expect(3) = 4e-082
Identities = 61/83 (73%), Positives = 71/83 (85%)
Frame = -1 / +2
Query: 1007 EKSLQKAVANQPVSVAIEAAGTAFQLYSSGIFTGSCGTALDHGVTAVGYGTENGKDYWIV 828
E +LQKAVANQP++VA+E G FQLY G+FTG CGTALDHGV AVGYGTENGKDYWIV
Sbjct: 5 ELALQKAVANQPIAVAVEGGGREFQLYEYGVFTGRCGTALDHGVAAVGYGTENGKDYWIV 184
Query: 827 KNSWGSSWGESGYVRMERNIKAS 759
+NSWG SWGE GY+R+ERN+ +S
Sbjct: 185 RNSWGGSWGEQGYIRLERNLASS 253
Score = 137 bits (294), Expect(3) = 4e-082
Identities = 42/58 (72%), Positives = 49/58 (84%)
Frame = -1 / +2
Query: 656 AVCDNYYSCPDSTTCCCIYEYGKYCFAWGCCPLEGATCCDDHYSCCPHDYPICNVRQG 483
+VCD+YYSC + +TCCCIYEYG CF WGCCPLE ATCCDD YSCCPH+YP+C+ R G
Sbjct: 359 SVCDSYYSCAEGSTCCCIYEYGGSCFEWGCCPLESATCCDDRYSCCPHEYPVCDTRAG 532
Score = 116 bits (248), Expect(3) = 2e-031
Identities = 51/76 (67%), Positives = 55/76 (72%)
Frame = +1 / -3
Query: 772 LRSILTYPDSPQLLPHEFFTIQ*SLPFSVP*PTAVTPWSSAVPQLPVKIPEL*S*NAVPA 951
LRS L P SPQL PHEF TIQ*S PFSVP PTA TPWSSAVP LPV P + N++P
Sbjct: 240 LRSSLM*PCSPQLPPHEFLTIQ*SFPFSVPYPTAATPWSSAVPHLPVNTPYSYNWNSLPP 61
Query: 952 ASIATLTGWFATAFCR 999
S AT GWFATAFC+
Sbjct: 60 PSTATAIGWFATAFCK 13
Score = 37.3 bits (75), Expect(3) = 4e-082
Identities = 12/15 (80%), Positives = 15/15 (100%)
Frame = -1 / +2
Query: 758 SGKCGIAVEPSYPLK 714
+GKCGIA+EPSYP+K
Sbjct: 257 AGKCGIAIEPSYPIK 301
Score = 27.6 bits (54), Expect(3) = 2e-031
Identities = 16/25 (64%), Positives = 17/25 (68%)
Frame = +1 / -3
Query: 580 KQYLPYS*MQQQVVLSGHE**LSHT 654
KQ PYS*+QQQV S **LS T
Sbjct: 435 KQDPPYS*IQQQVEPSAQL**LSQT 361
Score = 25.4 bits (49), Expect(3) = 2e-031
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = +1 / -3
Query: 709 PSFNGYDGSTAIPHLP 756
P G DGS AIPH P
Sbjct: 306 PFLIG*DGSIAIPHFP 259
>gb|CO515300.1|CO515300 s13dSG48C1200098_417659 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 588
Score = 127 bits (271), Expect(4) = 1e-075
Identities = 54/72 (75%), Positives = 57/72 (79%)
Frame = -3 / +2
Query: 1392 RYHAADNEDLPESVDWRAKGAVAEVKDQGSYGSCWAFSTIAAVEGINQIVTGDLISLSEQ 1213
RY + LPESVDWR +GAV VKDQ S GSCWAFS IAAVEGIN+IVTGDLISLSEQ
Sbjct: 269 RYAPRVGDKLPESVDWRKEGAVVGVKDQASCGSCWAFSAIAAVEGINKIVTGDLISLSEQ 448
Query: 1212 ELVDCDTSVQSG 1177
ELVDCDTS G
Sbjct: 449 ELVDCDTSYNEG 484
Score = 102 bits (217), Expect(4) = 3e-045
Identities = 46/65 (70%), Positives = 49/65 (75%)
Frame = +2 / -1
Query: 1178 PDCTEVSQSTSSCSDKEIKSPVTIWLMPSTAAIVEKAQQLP*LPWSLTSATAPLALQSTD 1357
P +VSQSTSSCSD EI+SPVTI +PSTAAI E AQQLP L WSLT TAP LQSTD
Sbjct: 483 PSLYDVSQSTSSCSDSEIRSPVTILFIPSTAAIAENAQQLPQLAWSLTPTTAPSFLQSTD 304
Query: 1358 SGRSS 1372
SG S
Sbjct: 303 SGNLS 289
Score = 90.9 bits (192), Expect(4) = 1e-075
Identities = 32/38 (84%), Positives = 36/38 (94%)
Frame = -1 / +2
Query: 1187 YNQGCNGGLMDYAFEFIINNGGIDTEKDYPYKGTDGRC 1074
YN+GCNGGLMDYAFEFII+NGGID+E+DYPYK DGRC
Sbjct: 473 YNEGCNGGLMDYAFEFIISNGGIDSEEDYPYKAIDGRC 586
Score = 66.6 bits (139), Expect(4) = 1e-075
Identities = 20/43 (46%), Positives = 34/43 (79%)
Frame = -3 / +2
Query: 1653 DEEARRMYAEWMAAHGRTYNAVGEEERRYQVFRDNLRYIDAHN 1525
+EE M EW+ HG++YN +GE+++R+++F+DNL++ID HN
Sbjct: 5 NEEVLTMCEEWLVKHGKSYNGLGEKDKRFEIFKDNLKFIDEHN 133
Score = 66.1 bits (138), Expect(4) = 3e-045
Identities = 30/39 (76%), Positives = 32/39 (82%)
Frame = +1 / -1
Query: 1072 SHRPSVPL*G*SFSVSIPPLLMMNSNA*SIRPPLHP*LY 1188
SH PS+ L*G*S S S+PPLLMMNS A*SI PPLHP LY
Sbjct: 588 SHLPSIAL*G*SSSESMPPLLMMNSKA*SISPPLHPSLY 472
Score = 52.4 bits (108), Expect(4) = 1e-075
Identities = 19/33 (57%), Positives = 25/33 (75%)
Frame = -3 / +2
Query: 1500 SFRLGLNRFADLTNDEYRATYLGARTRPQRERK 1402
++RLGL RFADLTN+EYR+ +LG + P R K
Sbjct: 146 TYRLGLTRFADLTNEEYRSKFLGTKINPNRRMK 244
Score = 33.6 bits (67), Expect(4) = 3e-045
Identities = 14/29 (48%), Positives = 19/29 (65%)
Frame = +2 / -1
Query: 1403 FLSLCGLVLAPR*VARYSSLVRSAKRLRP 1489
F+ GL+ P+ + YSSLVRSA R+ P
Sbjct: 243 FILRFGLIFVPKNLLLYSSLVRSANRVNP 157
Score = 31.8 bits (63), Expect(4) = 3e-045
Identities = 19/42 (45%), Positives = 22/42 (52%)
Frame = +2 / -1
Query: 1526 LCASM*RRLSRNTWYRRSSSPTAL*VRPCAAIHSAYIRRASS 1651
LC+SM RLS S SP L* PC HS++I SS
Sbjct: 132 LCSSMNLRLSLKISNLLSFSPKPL*DFPCFTNHSSHIVNTSS 7
>gb|CO514893.1|CO514893 s13dSG56F0300031_328446 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 620
Score = 159 bits (342), Expect(4) = 4e-063
Identities = 49/70 (70%), Positives = 60/70 (85%)
Frame = -1 / +1
Query: 656 AVCDNYYSCPDSTTCCCIYEYGKYCFAWGCCPLEGATCCDDHYSCCPHDYPICNVRQGTC 477
+VCD+YYSC + +TCCCIYEYG+ CF WGCCPLE ATCCDDHYSCCPH+YP+C+ R G C
Sbjct: 190 SVCDSYYSCAEGSTCCCIYEYGRSCFEWGCCPLESATCCDDHYSCCPHEYPVCDTRAGLC 369
Query: 476 LMGKDSPLSL 447
L GK++PL +
Sbjct: 370 LKGKNNPLGV 399
Score = 60.6 bits (126), Expect(4) = 4e-063
Identities = 19/27 (70%), Positives = 24/27 (88%)
Frame = -1 / +1
Query: 839 YWIVKNSWGSSWGESGYVRMERNIKAS 759
YWIV+NSWG SWGE GY+R+ERN+ +S
Sbjct: 4 YWIVRNSWGGSWGEQGYIRLERNLASS 84
Score = 37.3 bits (75), Expect(4) = 4e-063
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = -1 / +1
Query: 449 LSVKATKRTLAKPHWAFSG 393
L VK+ KRT AKPHWAF G
Sbjct: 391 LGVKSFKRTPAKPHWAFGG 447
Score = 37.3 bits (75), Expect(4) = 4e-063
Identities = 12/15 (80%), Positives = 15/15 (100%)
Frame = -1 / +1
Query: 758 SGKCGIAVEPSYPLK 714
+GKCGIA+EPSYP+K
Sbjct: 88 AGKCGIAIEPSYPIK 132
>gb|CO512362.1|CO512362 s13dSG80A0800053_114238 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 576
Score = 105 bits (223), Expect(3) = 3e-037
Identities = 41/58 (70%), Positives = 46/58 (79%)
Frame = -3 / +1
Query: 1368 DLPESVDWRAKGAVAEVKDQGSYGSCWAFSTIAAVEGINQIVTGDLISLSEQELVDCD 1195
+LPE DWR KGAV VKDQGS GSCWAFST A+EG N + TG L+SLSEQ+LVDCD
Sbjct: 274 NLPEDFDWREKGAVTPVKDQGSCGSCWAFSTTGALEGANYLATGKLVSLSEQQLVDCD 447
Score = 55.1 bits (114), Expect(3) = 3e-037
Identities = 19/32 (59%), Positives = 26/32 (81%)
Frame = -1 / +1
Query: 1184 NQGCNGGLMDYAFEFIINNGGIDTEKDYPYKG 1089
+ GCNGGLM+ AFE+I+ +GG+ +EKDY Y G
Sbjct: 481 DSGCNGGLMNNAFEYILQSGGVVSEKDYAYTG 576
Score = 29.0 bits (57), Expect(3) = 3e-037
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -3 / +1
Query: 1488 GLNRFADLTNDEYRATYLGARTR 1420
G+ +F+DLT E+R +LG R
Sbjct: 157 GITKFSDLTASEFRRQFLGLNKR 225
>emb|AJ245868.1|MSA245868 Medicago sativa mRNA for cysteine protease, partial (cyp15a gene)
Length = 890
Score = 63.8 bits (133), Expect(4) = 4e-032
Identities = 22/37 (59%), Positives = 29/37 (78%)
Frame = -1 / +1
Query: 1184 NQGCNGGLMDYAFEFIINNGGIDTEKDYPYKGTDGRC 1074
+ GCNGGLM+ AFE+I+ +GG+ +EKDY Y G DG C
Sbjct: 142 DSGCNGGLMNNAFEYILQSGGVVSEKDYAYTGRDGSC 252
Score = 61.6 bits (128), Expect(4) = 4e-032
Identities = 24/35 (68%), Positives = 28/35 (80%)
Frame = -3 / +1
Query: 1299 GSCWAFSTIAAVEGINQIVTGDLISLSEQELVDCD 1195
GS WAFST A+EG N + TG L+SLSEQ+LVDCD
Sbjct: 4 GSGWAFSTTGALEGANYLATGKLVSLSEQQLVDCD 108
Score = 48.7 bits (100), Expect(4) = 4e-032
Identities = 15/24 (62%), Positives = 19/24 (79%)
Frame = -1 / +1
Query: 845 KDYWIVKNSWGSSWGESGYVRMER 774
K YWI+KNSWG +WGE GY ++ R
Sbjct: 499 KPYWIIKNSWGQNWGEEGYYKICR 570
Score = 24.0 bits (46), Expect(4) = 4e-032
Identities = 8/12 (66%), Positives = 10/12 (83%)
Frame = -1 / +1
Query: 890 LDHGVTAVGYGT 855
LDHGV VG+G+
Sbjct: 433 LDHGVLLVGFGS 468
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,177,654
Number of Sequences: 7669
Number of extensions: 176329
Number of successful extensions: 1695
Number of sequences better than 1.0e-020: 7
length of database: 1,248,568
effective HSP length: 45
effective length of database: 903,463
effective search space used: 519491225
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)