TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2493944.2.1
(2055 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF537205.1| Medicago sativa apoplastic invertase-like mR... 136 2e-062
>gb|AF537205.1| Medicago sativa apoplastic invertase-like mRNA, partial sequence
Length = 714
Score = 136 bits (291), Expect(5) = 2e-062
Identities = 51/86 (59%), Positives = 64/86 (74%)
Frame = -1 / +1
Query: 1065 DEHRLRYDYGNFYASKTFYDPAKRRRILWGWANESDTAADDVAKGWAGIQAIPRTVWLDP 886
+++ LRYDYG +YASKTF+D K RRIL GWANES + DDV KGW+GI IPR +WL
Sbjct: 82 NQNVLRYDYGKYYASKTFFDDGKNRRILLGWANESSSVVDDVKKGWSGIHTIPRAIWLHK 261
Query: 885 SGKQLLQWPIEEVEALRAKSVTLRNR 808
SGKQL+QWP++E+E LR V N+
Sbjct: 262 SGKQLVQWPVKELENLRMNPVNWPNQ 339
Score = 77.1 bits (162), Expect(3) = 5e-028
Identities = 36/76 (47%), Positives = 45/76 (59%)
Frame = +2 / -2
Query: 812 FLRVTDFALSASTSSIGHCSSCFPLGSSHTVLGIAWIPAQPLATSSAAVSDSLAQPHRMR 991
F ++T F S+S GHC++CFP S LGI WIP P TSS + DSLAQP+++
Sbjct: 335 FGQLTGFIRRFSSSFTGHCTNCFPDL*SQIALGIVWIPDHPFLTSSTTLEDSLAQPNKIL 156
Query: 992 RRLAGS*NVLDA*KLP 1039
S NVLDA* P
Sbjct: 155 LFFPSSKNVLDA*YFP 108
Score = 62.0 bits (129), Expect(5) = 2e-062
Identities = 26/42 (61%), Positives = 31/42 (73%)
Frame = -1 / +2
Query: 678 DAERLCGVKRADVRGGVGPFGLWVLASANRKERTAVFFRVFK 553
D + LC K A V+GGVGPFGL VLAS +E TAVFFR+F+
Sbjct: 458 DPQILCSQKGASVKGGVGPFGLHVLASKGLQEYTAVFFRIFR 583
Score = 53.3 bits (110), Expect(3) = 5e-028
Identities = 24/37 (64%), Positives = 25/37 (67%)
Frame = +2 / -1
Query: 554 LNTLKNTAVLSLRLAEASTHSPNGPTPPLTSARFTPH 664
LN LKNTAV S + EAST PNGPTPP T A F H
Sbjct: 582 LNILKNTAVYSCKPLEASTCRPNGPTPPFTDAPF*LH 472
Score = 42.3 bits (86), Expect(5) = 2e-062
Identities = 19/40 (47%), Positives = 26/40 (65%)
Frame = -1 / +2
Query: 537 KPVVLMCTDPTKSSLNPNLYRPTFAGFVDTDISNGKISLR 418
K +VLMC+D +K+SLN T+ FVD D + K+SLR
Sbjct: 593 KNLVLMCSDQSKTSLNKENDMTTYGTFVDVDPLHEKLSLR 712
Score = 41.8 bits (85), Expect(5) = 2e-062
Identities = 15/29 (51%), Positives = 22/29 (75%)
Frame = -1 / +2
Query: 816 RNRVIKAGHHVEVTGIQTAQADVEVSFEV 730
+ +VIK G + +TG+ + QADVE+SFEV
Sbjct: 332 QTKVIKGGELIPITGVNSVQADVEISFEV 418
Score = 27.6 bits (54), Expect(3) = 5e-028
Identities = 14/27 (51%), Positives = 14/27 (51%)
Frame = +2 / -1
Query: 731 TSKLTSTSACAVCIPVTST*CPALITL 811
TS STSAC PV P LITL
Sbjct: 417 TSNEISTSACTELTPVIGINSPPLITL 337
Score = 27.6 bits (54), Expect(5) = 2e-062
Identities = 9/29 (31%), Positives = 17/29 (58%)
Frame = -1 / +1
Query: 1137 RYDYYTVGTYHPRAERYVPDDPAGDEHRL 1051
++D+Y +GTY + +VPD+ D +
Sbjct: 7 KHDHYLIGTYDTVKDVFVPDNGFEDNQNV 93
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 13,241,038
Number of Sequences: 7669
Number of extensions: 164603
Number of successful extensions: 1375
Number of sequences better than 1.0e-020: 1
length of database: 1,248,568
effective HSP length: 45
effective length of database: 903,463
effective search space used: 577312857
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)