TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2455940.2.2
(1190 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U20736.1|MSU20736 Medicago sativa S-adenosyl-L-methionin... 468 e-133
emb|AX259371.1| Sequence 1 from Patent WO0173090 468 e-133
emb|CQ760958.1| Sequence 3 from Patent WO2004002216 468 e-133
emb|CQ760964.1| Sequence 9 from Patent WO2004002216 468 e-133
gb|DQ122814.1| Medicago sativa clone C31 mRNA sequence 219 7e-058
>gb|U20736.1|MSU20736 Medicago sativa S-adenosyl-L-methionine:trans-caffeoyl-CoA
3-O-methyltransferase (CCOMT) mRNA, complete cds
Length = 966
Score = 468 bits (1016), Expect = e-133
Identities = 184/233 (78%), Positives = 212/233 (90%)
Frame = +3 / +3
Query: 186 RHSEVGHKSLLKSDDLYQYILDTSVYPREPESMKELREITAKHPWNLMTTSADEGQFLNM 365
RH EVGHKSLL+SD LYQYIL+TSV+PRE E+MKELRE+TAKHPWN+MTTSADEGQFL+M
Sbjct: 75 RHQEVGHKSLLQSDALYQYILETSVFPREHEAMKELREVTAKHPWNIMTTSADEGQFLSM 254
Query: 366 LIKLIGAKKTMEIGVYTGYSLLATALALPEDGTILAMDINRENYELGLPCINKAGVGHKI 545
L+KLI AK TMEIGVYTGYSLLATALA+PEDG ILAMDIN+ENYELGLP I KAGV HKI
Sbjct: 255 LLKLINAKNTMEIGVYTGYSLLATALAIPEDGKILAMDINKENYELGLPVIKKAGVDHKI 434
Query: 546 DFREGPALPVLDDLVADKEQHGSFDFAFVDADKDNYLSYHERLLKLVRPGGLIGYDNTLW 725
DFREGPALPVLD+++ D++ HGS+DF FVDADKDNYL+YH+RL+ LV+ GG+IGYDNTLW
Sbjct: 435 DFREGPALPVLDEMIKDEKNHGSYDFIFVDADKDNYLNYHKRLIDLVKVGGVIGYDNTLW 614
Query: 726 NGSVVLPDDAPMRKYIRFYRDFVLALNSALAADDRVEICQLPVGDGVTLCRRV 884
NGSVV P DAP+RKY+R+YRDFVL LN ALA D R+EIC LPVGDG+T+CRR+
Sbjct: 615 NGSVVAPPDAPLRKYVRYYRDFVLELNKALAVDPRIEICMLPVGDGITICRRI 773
Score = 183 bits (394), Expect(3) = 1e-056
Identities = 92/132 (69%), Positives = 100/132 (75%)
Frame = -2 / -3
Query: 631 TKAKSNDPCCSLSATRSSRTGSAGPSRKSILWPTPALLMQGRPSS*FSRLMSMAKIVPSS 452
TK KS P S SSRTG AGPS KSIL TPA L+ GRP+S*FS LMS+AKI PSS
Sbjct: 520 TKMKS*LP*FFSSLIISSRTGRAGPSLKSIL*STPAFLITGRPNS*FSLLMSIAKIFPSS 341
Query: 451 GSASAVARSE*PV*TPISMVFLAPMSLMSMLRNWPSSAEVVIRFHGCLAVISRSSFMLSG 272
G A AVAR E*PV*TPISMVFLA +SL SML+N PSSAEVV+ FHGC AV S +SFM S
Sbjct: 340 GIARAVARRE*PV*TPISMVFLALISLRSMLKNCPSSAEVVMMFHGCFAVTSLNSFMASC 161
Query: 271 SRGYTLVSRMYW 236
S G TLVSR+YW
Sbjct: 160 SLGKTLVSRIYW 125
Score = 45.5 bits (93), Expect(3) = 1e-056
Identities = 18/29 (62%), Positives = 21/29 (72%)
Frame = -2 / -3
Query: 769 YLRMGASSGSTTEPFHSVLS*PMRPPGLT 683
Y GAS G+TT+PFH VLS P+ PP LT
Sbjct: 658 YFLNGASGGATTDPFHKVLSYPITPPTLT 572
Score = 24.9 bits (48), Expect(3) = 1e-056
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = -2 / -3
Query: 889 HLTRRQSVTPSPTGSWQI 836
+L RQ V PSPTGS I
Sbjct: 778 YLILRQIVIPSPTGSIHI 725
>emb|AX259371.1| Sequence 1 from Patent WO0173090
Length = 744
Score = 468 bits (1016), Expect = e-133
Identities = 184/233 (78%), Positives = 212/233 (90%)
Frame = +3 / +1
Query: 186 RHSEVGHKSLLKSDDLYQYILDTSVYPREPESMKELREITAKHPWNLMTTSADEGQFLNM 365
RH EVGHKSLL+SD LYQYIL+TSV+PRE E+MKELRE+TAKHPWN+MTTSADEGQFL+M
Sbjct: 40 RHQEVGHKSLLQSDALYQYILETSVFPREHEAMKELREVTAKHPWNIMTTSADEGQFLSM 219
Query: 366 LIKLIGAKKTMEIGVYTGYSLLATALALPEDGTILAMDINRENYELGLPCINKAGVGHKI 545
L+KLI AK TMEIGVYTGYSLLATALA+PEDG ILAMDIN+ENYELGLP I KAGV HKI
Sbjct: 220 LLKLINAKNTMEIGVYTGYSLLATALAIPEDGKILAMDINKENYELGLPVIKKAGVDHKI 399
Query: 546 DFREGPALPVLDDLVADKEQHGSFDFAFVDADKDNYLSYHERLLKLVRPGGLIGYDNTLW 725
DFREGPALPVLD+++ D++ HGS+DF FVDADKDNYL+YH+RL+ LV+ GG+IGYDNTLW
Sbjct: 400 DFREGPALPVLDEMIKDEKNHGSYDFIFVDADKDNYLNYHKRLIDLVKVGGVIGYDNTLW 579
Query: 726 NGSVVLPDDAPMRKYIRFYRDFVLALNSALAADDRVEICQLPVGDGVTLCRRV 884
NGSVV P DAP+RKY+R+YRDFVL LN ALA D R+EIC LPVGDG+T+CRR+
Sbjct: 580 NGSVVAPPDAPLRKYVRYYRDFVLELNKALAVDPRIEICMLPVGDGITICRRI 738
Score = 183 bits (394), Expect(3) = 1e-056
Identities = 92/132 (69%), Positives = 100/132 (75%)
Frame = -2 / -2
Query: 631 TKAKSNDPCCSLSATRSSRTGSAGPSRKSILWPTPALLMQGRPSS*FSRLMSMAKIVPSS 452
TK KS P S SSRTG AGPS KSIL TPA L+ GRP+S*FS LMS+AKI PSS
Sbjct: 485 TKMKS*LP*FFSSLIISSRTGRAGPSLKSIL*STPAFLITGRPNS*FSLLMSIAKIFPSS 306
Query: 451 GSASAVARSE*PV*TPISMVFLAPMSLMSMLRNWPSSAEVVIRFHGCLAVISRSSFMLSG 272
G A AVAR E*PV*TPISMVFLA +SL SML+N PSSAEVV+ FHGC AV S +SFM S
Sbjct: 305 GIARAVARRE*PV*TPISMVFLALISLRSMLKNCPSSAEVVMMFHGCFAVTSLNSFMASC 126
Query: 271 SRGYTLVSRMYW 236
S G TLVSR+YW
Sbjct: 125 SLGKTLVSRIYW 90
Score = 45.5 bits (93), Expect(3) = 1e-056
Identities = 18/29 (62%), Positives = 21/29 (72%)
Frame = -2 / -2
Query: 769 YLRMGASSGSTTEPFHSVLS*PMRPPGLT 683
Y GAS G+TT+PFH VLS P+ PP LT
Sbjct: 623 YFLNGASGGATTDPFHKVLSYPITPPTLT 537
Score = 24.9 bits (48), Expect(3) = 1e-056
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = -2 / -2
Query: 889 HLTRRQSVTPSPTGSWQI 836
+L RQ V PSPTGS I
Sbjct: 743 YLILRQIVIPSPTGSIHI 690
>emb|CQ760958.1| Sequence 3 from Patent WO2004002216
Length = 744
Score = 468 bits (1016), Expect = e-133
Identities = 184/233 (78%), Positives = 212/233 (90%)
Frame = +3 / +1
Query: 186 RHSEVGHKSLLKSDDLYQYILDTSVYPREPESMKELREITAKHPWNLMTTSADEGQFLNM 365
RH EVGHKSLL+SD LYQYIL+TSV+PRE E+MKELRE+TAKHPWN+MTTSADEGQFL+M
Sbjct: 40 RHQEVGHKSLLQSDALYQYILETSVFPREHEAMKELREVTAKHPWNIMTTSADEGQFLSM 219
Query: 366 LIKLIGAKKTMEIGVYTGYSLLATALALPEDGTILAMDINRENYELGLPCINKAGVGHKI 545
L+KLI AK TMEIGVYTGYSLLATALA+PEDG ILAMDIN+ENYELGLP I KAGV HKI
Sbjct: 220 LLKLINAKNTMEIGVYTGYSLLATALAIPEDGKILAMDINKENYELGLPVIKKAGVDHKI 399
Query: 546 DFREGPALPVLDDLVADKEQHGSFDFAFVDADKDNYLSYHERLLKLVRPGGLIGYDNTLW 725
DFREGPALPVLD+++ D++ HGS+DF FVDADKDNYL+YH+RL+ LV+ GG+IGYDNTLW
Sbjct: 400 DFREGPALPVLDEMIKDEKNHGSYDFIFVDADKDNYLNYHKRLIDLVKVGGVIGYDNTLW 579
Query: 726 NGSVVLPDDAPMRKYIRFYRDFVLALNSALAADDRVEICQLPVGDGVTLCRRV 884
NGSVV P DAP+RKY+R+YRDFVL LN ALA D R+EIC LPVGDG+T+CRR+
Sbjct: 580 NGSVVAPPDAPLRKYVRYYRDFVLELNKALAVDPRIEICMLPVGDGITICRRI 738
Score = 183 bits (394), Expect(3) = 1e-056
Identities = 92/132 (69%), Positives = 100/132 (75%)
Frame = -2 / -2
Query: 631 TKAKSNDPCCSLSATRSSRTGSAGPSRKSILWPTPALLMQGRPSS*FSRLMSMAKIVPSS 452
TK KS P S SSRTG AGPS KSIL TPA L+ GRP+S*FS LMS+AKI PSS
Sbjct: 485 TKMKS*LP*FFSSLIISSRTGRAGPSLKSIL*STPAFLITGRPNS*FSLLMSIAKIFPSS 306
Query: 451 GSASAVARSE*PV*TPISMVFLAPMSLMSMLRNWPSSAEVVIRFHGCLAVISRSSFMLSG 272
G A AVAR E*PV*TPISMVFLA +SL SML+N PSSAEVV+ FHGC AV S +SFM S
Sbjct: 305 GIARAVARRE*PV*TPISMVFLALISLRSMLKNCPSSAEVVMMFHGCFAVTSLNSFMASC 126
Query: 271 SRGYTLVSRMYW 236
S G TLVSR+YW
Sbjct: 125 SLGKTLVSRIYW 90
Score = 45.5 bits (93), Expect(3) = 1e-056
Identities = 18/29 (62%), Positives = 21/29 (72%)
Frame = -2 / -2
Query: 769 YLRMGASSGSTTEPFHSVLS*PMRPPGLT 683
Y GAS G+TT+PFH VLS P+ PP LT
Sbjct: 623 YFLNGASGGATTDPFHKVLSYPITPPTLT 537
Score = 24.9 bits (48), Expect(3) = 1e-056
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = -2 / -2
Query: 889 HLTRRQSVTPSPTGSWQI 836
+L RQ V PSPTGS I
Sbjct: 743 YLILRQIVIPSPTGSIHI 690
>emb|CQ760964.1| Sequence 9 from Patent WO2004002216
Length = 1906
Score = 468 bits (1016), Expect = e-133
Identities = 184/233 (78%), Positives = 212/233 (90%)
Frame = +3 / +1
Query: 186 RHSEVGHKSLLKSDDLYQYILDTSVYPREPESMKELREITAKHPWNLMTTSADEGQFLNM 365
RH EVGHKSLL+SD LYQYIL+TSV+PRE E+MKELRE+TAKHPWN+MTTSADEGQFL+M
Sbjct: 1015 RHQEVGHKSLLQSDALYQYILETSVFPREHEAMKELREVTAKHPWNIMTTSADEGQFLSM 1194
Query: 366 LIKLIGAKKTMEIGVYTGYSLLATALALPEDGTILAMDINRENYELGLPCINKAGVGHKI 545
L+KLI AK TMEIGVYTGYSLLATALA+PEDG ILAMDIN+ENYELGLP I KAGV HKI
Sbjct: 1195 LLKLINAKNTMEIGVYTGYSLLATALAIPEDGKILAMDINKENYELGLPVIKKAGVDHKI 1374
Query: 546 DFREGPALPVLDDLVADKEQHGSFDFAFVDADKDNYLSYHERLLKLVRPGGLIGYDNTLW 725
DFREGPALPVLD+++ D++ HGS+DF FVDADKDNYL+YH+RL+ LV+ GG+IGYDNTLW
Sbjct: 1375 DFREGPALPVLDEMIKDEKNHGSYDFIFVDADKDNYLNYHKRLIDLVKVGGVIGYDNTLW 1554
Query: 726 NGSVVLPDDAPMRKYIRFYRDFVLALNSALAADDRVEICQLPVGDGVTLCRRV 884
NGSVV P DAP+RKY+R+YRDFVL LN ALA D R+EIC LPVGDG+T+CRR+
Sbjct: 1555 NGSVVAPPDAPLRKYVRYYRDFVLELNKALAVDPRIEICMLPVGDGITICRRI 1713
Score = 183 bits (394), Expect(3) = 1e-056
Identities = 92/132 (69%), Positives = 100/132 (75%)
Frame = -2 / -3
Query: 631 TKAKSNDPCCSLSATRSSRTGSAGPSRKSILWPTPALLMQGRPSS*FSRLMSMAKIVPSS 452
TK KS P S SSRTG AGPS KSIL TPA L+ GRP+S*FS LMS+AKI PSS
Sbjct: 1460 TKMKS*LP*FFSSLIISSRTGRAGPSLKSIL*STPAFLITGRPNS*FSLLMSIAKIFPSS 1281
Query: 451 GSASAVARSE*PV*TPISMVFLAPMSLMSMLRNWPSSAEVVIRFHGCLAVISRSSFMLSG 272
G A AVAR E*PV*TPISMVFLA +SL SML+N PSSAEVV+ FHGC AV S +SFM S
Sbjct: 1280 GIARAVARRE*PV*TPISMVFLALISLRSMLKNCPSSAEVVMMFHGCFAVTSLNSFMASC 1101
Query: 271 SRGYTLVSRMYW 236
S G TLVSR+YW
Sbjct: 1100 SLGKTLVSRIYW 1065
Score = 45.5 bits (93), Expect(3) = 1e-056
Identities = 18/29 (62%), Positives = 21/29 (72%)
Frame = -2 / -3
Query: 769 YLRMGASSGSTTEPFHSVLS*PMRPPGLT 683
Y GAS G+TT+PFH VLS P+ PP LT
Sbjct: 1598 YFLNGASGGATTDPFHKVLSYPITPPTLT 1512
Score = 24.9 bits (48), Expect(3) = 1e-056
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = -2 / -3
Query: 889 HLTRRQSVTPSPTGSWQI 836
+L RQ V PSPTGS I
Sbjct: 1718 YLILRQIVIPSPTGSIHI 1665
>gb|DQ122814.1| Medicago sativa clone C31 mRNA sequence
Length = 562
Score = 219 bits (472), Expect = 7e-058
Identities = 85/143 (59%), Positives = 111/143 (77%)
Frame = +3 / +2
Query: 213 LLKSDDLYQYILDTSVYPREPESMKELREITAKHPWNLMTTSADEGQFLNMLIKLIGAKK 392
+L+S+DL +YILDT+VYPREPE +KELR T HPW + T + GQ + +L+KL+ KK
Sbjct: 134 ILQSEDLTKYILDTAVYPREPEPLKELRNATENHPWGFIATLPEAGQLITLLLKLLNPKK 313
Query: 393 TMEIGVYTGYSLLATALALPEDGTILAMDINRENYELGLPCINKAGVGHKIDFREGPALP 572
T+E+GV+TGYSLL TAL +P+DG I A+DI+R+ YE+GLP I KAGV HKID+ E PALP
Sbjct: 314 TIEVGVFTGYSLLLTALNIPDDGKITAIDIDRKTYEIGLPVIRKAGVEHKIDYIESPALP 493
Query: 573 VLDDLVADKEQHGSFDFAFVDAD 641
+LD L+ D G+FDFAF+DAD
Sbjct: 494 ILDKLLEDPTNEGTFDFAFIDAD 562
Score = 103 bits (219), Expect = 5e-023
Identities = 62/131 (47%), Positives = 76/131 (58%)
Frame = -2 / -2
Query: 628 KAKSNDPCCSLSATRSSRTGSAGPSRKSILWPTPALLMQGRPSS*FSRLMSMAKIVPSSG 449
KAKSN P S++ S+ G AG S SIL TPA LM G P S* +SMA I PSSG
Sbjct: 549 KAKSNVPSFVGSSSSLSKIGRAGDSM*SILCSTPAFLMTGSPIS*VFLSISMAVIFPSSG 370
Query: 448 SASAVARSE*PV*TPISMVFLAPMSLMSMLRNWPSSAEVVIRFHGCLAVISRSSFMLSGS 269
SAV R E PV TP S+VFL S S + + P+S +V ++ G +V SS SGS
Sbjct: 369 MLSAVRRREYPVNTPTSIVFLGFNSFKSRVIS*PASGKVAMKPQG*FSVAFLSSLRGSGS 190
Query: 268 RGYTLVSRMYW 236
G T VS +Y+
Sbjct: 189 LG*TAVSNIYF 157
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,779,647
Number of Sequences: 7669
Number of extensions: 57937
Number of successful extensions: 1141
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 324336753
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)