TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2455828.2.1
(1565 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|AJ000995.1|MSDNAJ Medicago sativa mRNA for DnaJ-like pr... 67 1e-026
gb|AF069507.1|AF069507 Medicago sativa DnaJ-like protein Ms... 53 5e-022
>emb|AJ000995.1|MSDNAJ Medicago sativa mRNA for DnaJ-like protein
Length = 1545
Score = 66.6 bits (139), Expect(3) = 1e-026
Identities = 30/97 (30%), Positives = 49/97 (50%)
Frame = -2 / +1
Query: 676 KEFCKACRGNKVVPGRKSVRLDILPGSDNEDTIKVVRSGGEDPDGRPGDLYVTLEVREDP 497
++ C C+GNK+ +K + + + G I E PD GD+ L+V+ P
Sbjct: 673 RDRCPQCKGNKITQEKKVLEVHVEKGMQQGHKIVFEGQADELPDTITGDIVFVLQVKGHP 852
Query: 496 VFRREKGDIHVDAVLNVTQAILGGTVQVPTLSGDVVL 386
FRRE+ D+H++ L++T A+ G V L G +L
Sbjct: 853 KFRRERDDLHIEHNLSLTDALCGFQFNVTHLDGRQLL 963
Score = 53.3 bits (110), Expect(3) = 1e-026
Identities = 24/38 (63%), Positives = 26/38 (68%)
Frame = -2 / +1
Query: 1288 TRPVAARDYYDVLGVSKSASQAEIKKAYYGLAKKLHPD 1175
TR YYD+LGVSKSAS+ EIKKAY A K HPD
Sbjct: 76 TRKSDNTKYYDILGVSKSASEDEIKKAYRKAAMKNHPD 189
Score = 40.9 bits (83), Expect(3) = 1e-026
Identities = 15/27 (55%), Positives = 20/27 (74%)
Frame = -2 / +1
Query: 1144 KFQEVQRAYETLKDEQKRSLYDQVGPD 1064
KF+E+ +AYE L D +K+ LYDQ G D
Sbjct: 208 KFKELGQAYEVLSDPEKKELYDQYGED 288
>gb|AF069507.1|AF069507 Medicago sativa DnaJ-like protein MsJ1 gene, complete cds
Length = 4449
Score = 53.3 bits (110), Expect(4) = 5e-022
Identities = 24/38 (63%), Positives = 26/38 (68%)
Frame = -2 / +1
Query: 1288 TRPVAARDYYDVLGVSKSASQAEIKKAYYGLAKKLHPD 1175
TR YYD+LGVSKSAS+ EIKKAY A K HPD
Sbjct: 76 TRKSDNTKYYDILGVSKSASEDEIKKAYRKAAMKNHPD 189
Score = 48.7 bits (100), Expect(4) = 5e-022
Identities = 21/55 (38%), Positives = 32/55 (58%)
Frame = -2 / +3
Query: 550 PDGRPGDLYVTLEVREDPVFRREKGDIHVDAVLNVTQAILGGTVQVPTLSGDVVL 386
PD GD+ L+V+ P FRRE+ D+H++ L++T A+ G V L G +L
Sbjct: 2913 PDTITGDIVFVLQVKGHPKFRRERDDLHIEHNLSLTDALCGFQFNVTHLDGRQLL 3077
Score = 39.1 bits (79), Expect(4) = 5e-022
Identities = 14/27 (51%), Positives = 20/27 (74%)
Frame = -2 / +1
Query: 1144 KFQEVQRAYETLKDEQKRSLYDQVGPD 1064
+F+E+ +AYE L D +K+ LYDQ G D
Sbjct: 979 QFKELGQAYEVLSDPEKKELYDQYGED 1059
Score = 28.1 bits (55), Expect(4) = 5e-022
Identities = 10/39 (25%), Positives = 20/39 (51%)
Frame = -2 / +2
Query: 715 STCTKCGGSGKTVKEFCKACRGNKVVPGRKSVRLDILPG 599
S+C G + ++ C C+GNK+ +K + + + G
Sbjct: 2591 SSCIIVAGEVISERDRCPQCKGNKITQEKKVLEVHVEKG 2707
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,852,361
Number of Sequences: 7669
Number of extensions: 189099
Number of successful extensions: 1241
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 434609964
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)