TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 2441599.2.2
         (767 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AJ888496.1|AJ888496  AJ888496 Medicago sativa subsp. x va...   178   7e-049

>gb|AJ888496.1|AJ888496 AJ888496 Medicago sativa subsp. x varia 4-day-old Medicago sativa
           subsp. x varia cDNA, mRNA sequence
          Length = 470

 Score =  178 bits (383), Expect(2) = 7e-049
 Identities = 72/110 (65%), Positives = 83/110 (75%)
 Frame = +1 / +1

                                                                       
Query: 109 GEVMAMDPDAVAKAFVEHYYRTFDTNRAALVGLYQETSMLTFEGQKFQGPSAIAGKLGSL 288
           G V AMDP+A++KAFVEHYY TFDTNR  L  LYQ+ SMLTFEGQ+  G   I  KL SL
Sbjct: 31  GGVRAMDPNALSKAFVEHYYTTFDTNRPNLATLYQDGSMLTFEGQQIMGSQNIVAKLTSL 210

                                                             
Query: 289 PFQACEHQIVTVDCQPSGPQGGMLVFVSGSIRTGPEEHPIKFSQAFHLLP 438
           PFQ C H I TVDCQPSG  GGMLVFVSG+++   E+H +KFSQ FHL+P
Sbjct: 211 PFQQCHHSITTVDCQPSGANGGMLVFVSGNLQLAGEQHALKFSQMFHLIP 360




 Score = 98.2 bits (208), Expect(2) = 2e-023
 Identities = 55/110 (50%), Positives = 61/110 (55%)
 Frame = -1 / -1

                                                                       
Query: 437 GSKWNAWENLMGCSSGPVRMEPETKTSMPPWGPDGWQSTVTIWCSQAWKGRDPSLPAMAD 258
           G KWN   NL  C S      P TKTSMPP  P+G QSTV +     WKGR+ SL  +  
Sbjct: 359 GIKWNI*LNLRACCSPASWRLPLTKTSMPPLAPEG*QSTVVME*WHCWKGREVSLATIF* 180

                                                             
Query: 257 GPWNFWPSKVSMEVS*YSPTSAARLVSNVR**CSTKAFATASGSIAITSP 108
            P    PSKVS+E S        RLVS V **CST AF  A GSIA+T P
Sbjct: 179 DPIIC*PSKVSIEPSW*RVARLGRLVSKVV**CSTNAFDNAFGSIALTPP 30




 Score = 30.4 bits (60), Expect(2) = 7e-049
 Identities = 11/16 (68%), Positives = 14/16 (87%)
 Frame = +1 / +1

                           
Query: 445 GSFFVQNDMFRLNYG* 492
           GS++V ND+FRLNY *
Sbjct: 370 GSYYVLNDIFRLNYA* 417




 Score = 25.4 bits (49), Expect(2) = 2e-023
 Identities = 8/11 (72%), Positives = 9/11 (81%)
 Frame = -2 / -2

                      
Query: 493 FSHSSGGTCHS 461
           F+HSS G CHS
Sbjct: 418 FTHSSNGICHS 386



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 5,905,891
Number of Sequences: 7669
Number of extensions: 75169
Number of successful extensions: 856
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 197338110
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)