TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440568.2.1
(1135 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|AX776636.1| Sequence 5 from Patent WO02102841 170 3e-059
emb|AJ418368.1|MSA418368 Medicago sativa mRNA for nodulatio... 170 3e-059
emb|AX776635.1| Sequence 4 from Patent WO02102841 166 8e-053
emb|AJ418377.1|MSA418377 Medicago sativa mRNA for nodulatio... 166 8e-053
gb|CO511940.1|CO511940 s13dSG05G1200100_103780 Glandular tr... 105 2e-046
gb|CO512543.1|CO512543 s13dSG08D0400030_121012 Glandular tr... 138 4e-044
gb|AY563141.1| Medicago sativa calcium/calmodulin-regulated... 128 3e-035
gb|CO512977.1|CO512977 s13dSG09B0200013_121880 Glandular tr... 64 5e-028
gb|CO511959.1|CO511959 s13dSG07B0300029_103818 Glandular tr... 58 4e-021
>emb|AX776636.1| Sequence 5 from Patent WO02102841
Length = 3339
Score = 170 bits (366), Expect(3) = 3e-059
Identities = 80/183 (43%), Positives = 106/183 (57%)
Frame = -1 / +3
Query: 814 VLSWSQRVKIAVGAAKGLEYLHEKAQPHIIHRDIKSSNVLLFDDDVAKIADFDLSNQAPD 635
+L W R+ IA+GAA+GL YLH +IHRD+KSSN+LL AK+ADF S AP
Sbjct: 2313 ILDWPTRLSIALGAARGLAYLHTFPGRSVIHRDVKSSNILLDQSMCAKVADFGFSKYAPQ 2492
Query: 634 MAARLHSTRVLGTFGYHAPEYAMTGQLSSKSDVYSFGVVLLELLTGRKPVDHTLPRGQQS 455
S V GT GY PEY T QLS KSDV+SFGVVLLE+++GR+P++ PR + S
Sbjct: 2493 EGDSYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEWS 2672
Query: 454 LVTWATPRLSEDKVRQCVDSRLGGDYPPXXXXXXXXXXALCVQYEADFRPNMSIVVKALQ 275
LV WA P + KV + VD + G Y C++ + +RP M +V+ L+
Sbjct: 2673 LVEWAKPYIRASKVDEIVDPGIKGGYHAEALWRVVEVALQCLEPYSTYRPCMVDIVRELE 2852
Query: 274 PLL 266
L
Sbjct: 2853 DAL 2861
Score = 54.7 bits (113), Expect(3) = 3e-059
Identities = 22/55 (40%), Positives = 37/55 (67%)
Frame = -1 / +3
Query: 1012 SNKQPDQEFLAQVSMVSRLKHENVVELLGYCADGTLRVLAYEFATMGSLHDMLHG 848
++ Q EF +++++S ++HEN+V LLGYC + ++L Y F + GSL D L+G
Sbjct: 2130 TSTQGTXEFDNELNLLSAIQHENLVPLLGYCNEYDQQILVYPFMSNGSLLDRLYG 2294
Score = 37.3 bits (75), Expect(3) = 3e-059
Identities = 15/26 (57%), Positives = 18/26 (69%)
Frame = -1 / +3
Query: 1099 EALIGEGSFGRVYLGVLRNGRSAAVK 1022
+ LIGEG FG VY G L +G+ AVK
Sbjct: 2040 KTLIGEGGFGSVYRGTLDDGQEVAVK 2117
>emb|AJ418368.1|MSA418368 Medicago sativa mRNA for nodulation receptor kinase (nork gene), wild
type allele DMI2
Length = 3339
Score = 170 bits (366), Expect(3) = 3e-059
Identities = 80/183 (43%), Positives = 106/183 (57%)
Frame = -1 / +3
Query: 814 VLSWSQRVKIAVGAAKGLEYLHEKAQPHIIHRDIKSSNVLLFDDDVAKIADFDLSNQAPD 635
+L W R+ IA+GAA+GL YLH +IHRD+KSSN+LL AK+ADF S AP
Sbjct: 2313 ILDWPTRLSIALGAARGLAYLHTFPGRSVIHRDVKSSNILLDQSMCAKVADFGFSKYAPQ 2492
Query: 634 MAARLHSTRVLGTFGYHAPEYAMTGQLSSKSDVYSFGVVLLELLTGRKPVDHTLPRGQQS 455
S V GT GY PEY T QLS KSDV+SFGVVLLE+++GR+P++ PR + S
Sbjct: 2493 EGDSYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEWS 2672
Query: 454 LVTWATPRLSEDKVRQCVDSRLGGDYPPXXXXXXXXXXALCVQYEADFRPNMSIVVKALQ 275
LV WA P + KV + VD + G Y C++ + +RP M +V+ L+
Sbjct: 2673 LVEWAKPYIRASKVDEIVDPGIKGGYHAEALWRVVEVALQCLEPYSTYRPCMVDIVRELE 2852
Query: 274 PLL 266
L
Sbjct: 2853 DAL 2861
Score = 54.7 bits (113), Expect(3) = 3e-059
Identities = 22/55 (40%), Positives = 37/55 (67%)
Frame = -1 / +3
Query: 1012 SNKQPDQEFLAQVSMVSRLKHENVVELLGYCADGTLRVLAYEFATMGSLHDMLHG 848
++ Q EF +++++S ++HEN+V LLGYC + ++L Y F + GSL D L+G
Sbjct: 2130 TSTQGTXEFDNELNLLSAIQHENLVPLLGYCNEYDQQILVYPFMSNGSLLDRLYG 2294
Score = 37.3 bits (75), Expect(3) = 3e-059
Identities = 15/26 (57%), Positives = 18/26 (69%)
Frame = -1 / +3
Query: 1099 EALIGEGSFGRVYLGVLRNGRSAAVK 1022
+ LIGEG FG VY G L +G+ AVK
Sbjct: 2040 KTLIGEGGFGSVYRGTLDDGQEVAVK 2117
>emb|AX776635.1| Sequence 4 from Patent WO02102841
Length = 3378
Score = 166 bits (358), Expect(2) = 8e-053
Identities = 79/183 (43%), Positives = 105/183 (57%)
Frame = -1 / +1
Query: 814 VLSWSQRVKIAVGAAKGLEYLHEKAQPHIIHRDIKSSNVLLFDDDVAKIADFDLSNQAPD 635
+L W R+ IA+GA +GL LH P +IHRD+KSSN+LL AK+ADF S AP
Sbjct: 2332 ILDWPTRLSIALGAPRGLA*LHTFPGPSVIHRDLKSSNILLDQSMCAKVADFGFSKYAPQ 2511
Query: 634 MAARLHSTRVLGTFGYHAPEYAMTGQLSSKSDVYSFGVVLLELLTGRKPVDHTLPRGQQS 455
S V GT GY PEY T QLS KSDV+SFGVVLLE+++GR+P++ PR + S
Sbjct: 2512 EGDSYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEWS 2691
Query: 454 LVTWATPRLSEDKVRQCVDSRLGGDYPPXXXXXXXXXXALCVQYEADFRPNMSIVVKALQ 275
LV WA P + KV + VD + G Y C++ + +RP M +V+ L+
Sbjct: 2692 LVEWAKPYIRASKVDEIVDPGIKGGYHAEALWRVVEVALQCLEPYSTYRPCMVDIVRELE 2871
Query: 274 PLL 266
L
Sbjct: 2872 DAL 2880
Score = 55.6 bits (115), Expect(2) = 8e-053
Identities = 22/55 (40%), Positives = 38/55 (69%)
Frame = -1 / +1
Query: 1012 SNKQPDQEFLAQVSMVSRLKHENVVELLGYCADGTLRVLAYEFATMGSLHDMLHG 848
++ Q +EF +++++S ++HEN+V LLGYC + ++L Y F + GSL D L+G
Sbjct: 2149 TSTQGTKEFDNELNLLSAIQHENLVPLLGYCNEYDQQILVYPFMSNGSLLDRLYG 2313
>emb|AJ418377.1|MSA418377 Medicago sativa mRNA for nodulation receptor kinase (nork gene),
mutant allele nn1
Length = 3337
Score = 166 bits (358), Expect(2) = 8e-053
Identities = 79/183 (43%), Positives = 105/183 (57%)
Frame = -1 / +1
Query: 814 VLSWSQRVKIAVGAAKGLEYLHEKAQPHIIHRDIKSSNVLLFDDDVAKIADFDLSNQAPD 635
+L W R+ IA+GA +GL LH P +IHRD+KSSN+LL AK+ADF S AP
Sbjct: 2311 ILDWPTRLSIALGAPRGLA*LHTFPGPSVIHRDLKSSNILLDQSMCAKVADFGFSKYAPQ 2490
Query: 634 MAARLHSTRVLGTFGYHAPEYAMTGQLSSKSDVYSFGVVLLELLTGRKPVDHTLPRGQQS 455
S V GT GY PEY T QLS KSDV+SFGVVLLE+++GR+P++ PR + S
Sbjct: 2491 EGDSYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEWS 2670
Query: 454 LVTWATPRLSEDKVRQCVDSRLGGDYPPXXXXXXXXXXALCVQYEADFRPNMSIVVKALQ 275
LV WA P + KV + VD + G Y C++ + +RP M +V+ L+
Sbjct: 2671 LVEWAKPYIRASKVDEIVDPGIKGGYHAEALWRVVEVALQCLEPYSTYRPCMVDIVRELE 2850
Query: 274 PLL 266
L
Sbjct: 2851 DAL 2859
Score = 55.6 bits (115), Expect(2) = 8e-053
Identities = 22/55 (40%), Positives = 38/55 (69%)
Frame = -1 / +1
Query: 1012 SNKQPDQEFLAQVSMVSRLKHENVVELLGYCADGTLRVLAYEFATMGSLHDMLHG 848
++ Q +EF +++++S ++HEN+V LLGYC + ++L Y F + GSL D L+G
Sbjct: 2128 TSTQGTKEFDNELNLLSAIQHENLVPLLGYCNEYDQQILVYPFMSNGSLLDRLYG 2292
>gb|CO511940.1|CO511940 s13dSG05G1200100_103780 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 595
Score = 105 bits (225), Expect(3) = 2e-046
Identities = 41/61 (67%), Positives = 50/61 (81%)
Frame = -1 / +1
Query: 616 STRVLGTFGYHAPEYAMTGQLSSKSDVYSFGVVLLELLTGRKPVDHTLPRGQQSLVTWAT 437
STRV+GT+GY AP+YAMTGQL+ KSD+YS GV LLEL+TGRK +DH P +Q+LV WA
Sbjct: 247 STRVMGTYGYCAPDYAMTGQLTFKSDIYSIGVALLELITGRKAIDHRRPAKEQNLVAWAR 426
Query: 436 P 434
P
Sbjct: 427 P 429
Score = 81.7 bits (172), Expect(3) = 2e-046
Identities = 31/58 (53%), Positives = 45/58 (77%)
Frame = -1 / +1
Query: 811 LSWSQRVKIAVGAAKGLEYLHEKAQPHIIHRDIKSSNVLLFDDDVAKIADFDLSNQAP 638
L W+ R+KIA GAA+GLEYLH+K +P +I+RD+K SN+LL +D +K++DF L+ P
Sbjct: 52 LDWNTRMKIAAGAARGLEYLHDKMKPPVIYRDLKCSNILLGEDYHSKLSDFGLAKVGP 225
Score = 31.8 bits (63), Expect(3) = 2e-046
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = -1 / +1
Query: 409 QCVDSRLGGDYPPXXXXXXXXXXALCVQYEADFRPNMSIVVKAL 278
+ +D L G YP A+CVQ + + RP ++ VV AL
Sbjct: 457 EMIDPLLEGQYPVRGLYQALAIAAMCVQEQPNMRPVIADVVTAL 588
>gb|CO512543.1|CO512543 s13dSG08D0400030_121012 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 611
Score = 138 bits (295), Expect(2) = 4e-044
Identities = 61/114 (53%), Positives = 78/114 (68%)
Frame = -1 / +1
Query: 826 QPGPVLSWSQRVKIAVGAAKGLEYLHEKAQPHIIHRDIKSSNVLLFDDDVAKIADFDLSN 647
Q ++SW+ R+K+A+ AA+G+EYLH+ AQP IIHRDIK+SN+LL VAK++DF LS
Sbjct: 268 QTSTIMSWNGRIKVALDAARGIEYLHKYAQPPIIHRDIKTSNILLDSKWVAKVSDFGLSL 447
Query: 646 QAPDMAARLHSTRVLGTFGYHAPEYAMTGQLSSKSDVYSFGVVLLELLTGRKPV 485
P+ S GT GY PEY L+SKSDVYSFGV LLELL+G K +
Sbjct: 448 MGPEDEESHLSLLAAGTVGYMDPEYYRLQYLTSKSDVYSFGVXLLELLSGYKAI 609
Score = 55.6 bits (115), Expect(2) = 4e-044
Identities = 22/49 (44%), Positives = 33/49 (67%)
Frame = -1 / +1
Query: 997 DQEFLAQVSMVSRLKHENVVELLGYCADGTLRVLAYEFATMGSLHDMLH 851
D F+ ++ +SRL H+N+V+LLG+ D R+L YE+ GSL+D LH
Sbjct: 115 DSAFVNELESLSRLHHKNLVKLLGFYEDKNGRILVYEYMNNGSLNDHLH 261
>gb|AY563141.1| Medicago sativa calcium/calmodulin-regulated receptor-like kinase
mRNA, complete cds
Length = 1798
Score = 128 bits (274), Expect(2) = 3e-035
Identities = 61/144 (42%), Positives = 84/144 (58%)
Frame = -1 / +1
Query: 820 GPVLSWSQRVKIAVGAAKGLEYLHEKAQPHIIHRDIKSSNVLLFDDDVAKIADFDLSNQA 641
G L +R+ IA+ A + YLH IIHRDIK+SN+L+ ++ AK+ADF + +
Sbjct: 835 GDGLEIGERLDIAIDIAHAITYLHMYTDNPIIHRDIKASNILISENLRAKVADFGFARLS 1014
Query: 640 PDMAARLHSTRVLGTFGYHAPEYAMTGQLSSKSDVYSFGVVLLELLTGRKPVDHTLPRGQ 461
D A ST+V GT GY PEY T QL+ KSDVYSFGV+L+E++TGR PV+ +
Sbjct: 1015 EDPGATHISTQVKGTAGYMDPEYLRTYQLTEKSDVYSFGVLLVEMMTGRHPVEPKKKIDE 1194
Query: 460 QSLVTWATPRLSEDKVRQCVDSRL 389
+ + WA L +D RL
Sbjct: 1195 RVTIRWAMKMLKNGDAVFAMDPRL 1266
Score = 39.6 bits (80), Expect(2) = 3e-035
Identities = 18/38 (47%), Positives = 22/38 (57%)
Frame = -1 / +1
Query: 1132 EIREVTKGFGDEALIGEGSFGRVYLGVLRNGRSAAVKK 1019
EI + T F + IGEG FG VY G L +G AVK+
Sbjct: 538 EIYKSTAKFSSDNQIGEGGFGTVYRGKLNDGTIVAVKR 651
>gb|CO512977.1|CO512977 s13dSG09B0200013_121880 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 582
Score = 63.8 bits (133), Expect(3) = 5e-028
Identities = 28/57 (49%), Positives = 35/57 (61%)
Frame = -1 / +1
Query: 811 LSWSQRVKIAVGAAKGLEYLHEKAQPHIIHRDIKSSNVLLFDDDVAKIADFDLSNQA 641
L+WS R+KI G AKGL YLHE + +H D+K SN+LL D I+DF L A
Sbjct: 175 LTWSDRLKIMKGTAKGLVYLHEFSPKKYVHGDLKPSNILLGHDMTPYISDFGLGRLA 345
Score = 50.6 bits (104), Expect(3) = 5e-028
Identities = 20/36 (55%), Positives = 26/36 (72%)
Frame = -1 / +1
Query: 592 GYHAPEYAMTGQLSSKSDVYSFGVVLLELLTGRKPV 485
GY APE + S K DVYS+GV+LLE++TGR P+
Sbjct: 457 GYQAPEALKVVKPSQKWDVYSYGVILLEMITGRLPI 564
Score = 42.8 bits (87), Expect(3) = 5e-028
Identities = 16/53 (30%), Positives = 29/53 (54%)
Frame = -1 / +1
Query: 994 QEFLAQVSMVSRLKHENVVELLGYCADGTLRVLAYEFATMGSLHDMLHGRKGV 836
+EF +V + +L+H N+ L Y ++L Y++ GSL +HG+ G+
Sbjct: 1 REFQTEVEAIGKLRHPNIATLRAYYWSVDEKLLIYDYIPNGSLASAIHGKAGL 159
>gb|CO511959.1|CO511959 s13dSG07B0300029_103818 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 596
Score = 57.9 bits (120), Expect(4) = 4e-021
Identities = 28/55 (50%), Positives = 34/55 (61%)
Frame = -1 / +1
Query: 616 STRVLGTFGYHAPEYAMTGQLSSKSDVYSFGVVLLELLTGRKPVDHTLPRGQQSL 452
ST GT GY PEY QL+ KSD YSFGVVL+EL++ + VD T R +L
Sbjct: 421 STAPQGTPGYVDPEYYQCYQLTDKSDAYSFGVVLVELISSLRAVDITRHRNDVNL 585
Score = 39.6 bits (80), Expect(4) = 4e-021
Identities = 15/32 (46%), Positives = 23/32 (71%)
Frame = -1 / +1
Query: 730 IIHRDIKSSNVLLFDDDVAKIADFDLSNQAPD 635
++HRD+KS+N+LL + K+ADF LS P+
Sbjct: 310 VMHRDVKSNNILLDEKFHVKVADFGLSRLFPN 405
Score = 29.0 bits (57), Expect(4) = 4e-021
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = -1 / +1
Query: 814 VLSWSQRVKIAVGAAKGLEYLHEKAQPH 731
+L WS R+ IA+ A+ L YLH H
Sbjct: 235 LLPWSVRLDIALETAEALAYLHASDVMH 318
Score = 24.0 bits (46), Expect(4) = 4e-021
Identities = 8/18 (44%), Positives = 13/18 (72%)
Frame = -1 / +1
Query: 901 VLAYEFATMGSLHDMLHG 848
+L YE+ + G++ D LHG
Sbjct: 163 LLVYEYISNGTVADHLHG 216
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,761,992
Number of Sequences: 7669
Number of extensions: 141059
Number of successful extensions: 1294
Number of sequences better than 1.0e-020: 12
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 306879534
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)