TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419536.2.1
(1366 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF000976.1|MSAF000976 Medicago sativa 7-O-methyltransfer... 196 7e-065
gb|U97125.1|MSU97125 Medicago sativa isoflavone-O-methytran... 196 2e-063
emb|AX050436.1| Sequence 1 from Patent WO0071736 196 2e-063
gb|AF000975.1|MSAF000975 Medicago sativa 7-O-methyltransfer... 196 2e-063
gb|AF023481.1|AF023481 Medicago sativa o-methytransferase (... 99 4e-056
gb|M63853.1|ALFSALMCAT Medicago sativa S-adenosyl-L-methion... 117 2e-027
emb|AX259373.1| Sequence 3 from Patent WO0173090 117 2e-027
gb|L10211.1|ALFIOM Medicago sativa isoliquiritigenin 2'-O-m... 106 7e-024
gb|CO512262.1|CO512262 s13dSG03H0600048_114038 Glandular tr... 105 1e-023
>gb|AF000976.1|MSAF000976 Medicago sativa 7-O-methyltransferase (7-IOMT(9)) mRNA, complete cds
Length = 1227
Score = 196 bits (423), Expect(4) = 7e-065
Identities = 79/180 (43%), Positives = 118/180 (65%)
Frame = +2 / +3
Query: 599 IVDVAIKQSAEVFQGISSLVDVGGGIGTAAQAISKAFPHVKCSVLDLAHVVAKAPTHTDV 778
++++A++ VF G+ S+VDVGGG GT A+ I + FP +KC V D VV ++
Sbjct: 543 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 722
Query: 779 QFIAGDMFESIPPADAVLLKSVLHDWDHDDCVKILKNCKKAIPPREAGGKVIIINMVVGA 958
++ GDMF SIP ADAVLLK +LH+W DC++ILK CK+A+ GKV II+MV+
Sbjct: 723 TYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVINE 902
Query: 959 GPSDMKHKEMQAIFDVYIMFINGMERDEQEWSKIFSEAGYSDYRIIPVLGVRSIIEVYP* 1138
+ + +++ + DV + +NG ER+E+EW K+F EAG+ Y+I P+ G S+IE+YP*
Sbjct: 903 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1082
Score = 76.2 bits (160), Expect(3) = 5e-025
Identities = 50/116 (43%), Positives = 59/116 (50%)
Frame = -2 / -3
Query: 1137 YG*TSIIDRTPKTGIIL*SLYPASEKILLHSCSSRSMPLMNMI*TSNMACISLCFMSDGP 958
YG* SI D+ P G IL* PAS K HS S S P + TS + I + S
Sbjct: 1081 YG*ISIRDKNPVKGDIL*CWKPASMKSFFHSSSFLSFPFRQAMFTSIRSLI*VT*FSSFF 902
Query: 957 APTTMFIIITFPPASLGGIAFLQFFSIFTQSSWSQSCKTDFNSTASAGGMLSNMSP 790
+ TM II+TFP A L FFSI QS Q C+ + TASA G+ NMSP
Sbjct: 901 SFITMSIIVTFPLFPSFVTASLHFFSILRQSLSVQLCRIYLSKTASALGIDVNMSP 734
Score = 46.4 bits (95), Expect(3) = 5e-025
Identities = 20/49 (40%), Positives = 28/49 (57%)
Frame = -2 / -3
Query: 756 FATTWARSSTLHLTCGNAFEMAWAAVPMPPPTSTSELIPWKTSALCLMA 610
F+TT S+T+H G ++ A VP+PPPTST + P T + L A
Sbjct: 700 FSTTCGLSNTIHFNLGKVSQIILAVVPVPPPTSTIDSNPSNTKSQSLNA 554
Score = 46.4 bits (95), Expect(4) = 7e-065
Identities = 23/62 (37%), Positives = 34/62 (54%)
Frame = +2 / +3
Query: 35 QRIMELSPNNSTDQSLLDAQLELWHTTFAFMKSMALKSAIHLRIADAIHLHGGAASLSQI 214
Q+ M S N + AQ L+ +AF+ SM+LK A+ + I + IH HG SLS +
Sbjct: 15 QKKMASSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVGMNIPNIIHNHGKPISLSNL 194
Query: 215 LS 220
+S
Sbjct: 195 VS 200
Score = 34.5 bits (69), Expect(4) = 7e-065
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2 / +3
Query: 488 DPCIFKHTHGRGIWELTKDDATFDALVNDGLASDSQLI 601
D +F T G G W+ + ++ ND +ASDS+LI
Sbjct: 435 DLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSKLI 548
Score = 31.3 bits (62), Expect(3) = 5e-025
Identities = 17/61 (27%), Positives = 28/61 (45%)
Frame = -2 / -3
Query: 270 RIRRRRLETLDGWRWTLLSIWDRLAAPPWRWMASAIRRCIADLSAMDFMNAKVVCQSSSC 91
R+RRR L D + + ++R PW W+ + A L M+ M A + + +C
Sbjct: 250 RMRRRTLPIFDDGT*RIETKFEREIGLPWLWIMFGMFIPTAHLRDMESMKAYMCLYNKAC 71
Query: 90 A 88
A
Sbjct: 70 A 68
Score = 31.3 bits (62), Expect(4) = 7e-065
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = +2 / +3
Query: 383 QSSQLAQTPLAAMVLDPTIVSPFSELGAWFQHE 481
+ S L P+ VLDPT+ + EL W E
Sbjct: 336 RGSDLCLAPMVECVLDPTLSGSYHELKKWIYEE 434
>gb|U97125.1|MSU97125 Medicago sativa isoflavone-O-methytransferase mRNA, complete cds
Length = 1231
Score = 196 bits (422), Expect(4) = 2e-063
Identities = 79/180 (43%), Positives = 118/180 (65%)
Frame = +2 / +1
Query: 599 IVDVAIKQSAEVFQGISSLVDVGGGIGTAAQAISKAFPHVKCSVLDLAHVVAKAPTHTDV 778
++++A++ VF G+ S+VDVGGG GT A+ I + FP +KC V D VV ++
Sbjct: 544 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 723
Query: 779 QFIAGDMFESIPPADAVLLKSVLHDWDHDDCVKILKNCKKAIPPREAGGKVIIINMVVGA 958
++ GDMF SIP ADAVLLK +LH+W DC++ILK CK+A+ GKV II+MV+
Sbjct: 724 TYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIDK 903
Query: 959 GPSDMKHKEMQAIFDVYIMFINGMERDEQEWSKIFSEAGYSDYRIIPVLGVRSIIEVYP* 1138
+ + +++ + DV + +NG ER+E+EW K+F EAG+ Y+I P+ G S+IE+YP*
Sbjct: 904 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1083
Score = 76.7 bits (161), Expect(3) = 2e-025
Identities = 50/116 (43%), Positives = 58/116 (50%)
Frame = -2 / -3
Query: 1137 YG*TSIIDRTPKTGIIL*SLYPASEKILLHSCSSRSMPLMNMI*TSNMACISLCFMSDGP 958
YG* SI D+ P G IL* PAS K HS S S P + TS + I + S
Sbjct: 1082 YG*ISIRDKNPVKGDIL*CWKPASMKSFFHSSSFLSFPFRQAMFTSIRSLI*VT*FSSFF 903
Query: 957 APTTMFIIITFPPASLGGIAFLQFFSIFTQSSWSQSCKTDFNSTASAGGMLSNMSP 790
TM II+TFP A L FFSI QS Q C+ + TASA G+ NMSP
Sbjct: 902 LSITMSIIVTFPLFPSFVTASLHFFSILRQSLSVQLCRIYLSKTASALGIDVNMSP 735
Score = 46.4 bits (95), Expect(3) = 2e-025
Identities = 20/49 (40%), Positives = 28/49 (57%)
Frame = -2 / -3
Query: 756 FATTWARSSTLHLTCGNAFEMAWAAVPMPPPTSTSELIPWKTSALCLMA 610
F+TT S+T+H G ++ A VP+PPPTST + P T + L A
Sbjct: 701 FSTTCGLSNTIHFNLGKVSQIILAVVPVPPPTSTIDSNPSNTKSQSLNA 555
Score = 41.8 bits (85), Expect(4) = 2e-063
Identities = 21/59 (35%), Positives = 31/59 (52%)
Frame = +2 / +1
Query: 44 MELSPNNSTDQSLLDAQLELWHTTFAFMKSMALKSAIHLRIADAIHLHGGAASLSQILS 220
M S N + AQ L+ +AF+ SM+LK A+ + I + I HG SLS ++S
Sbjct: 25 MASSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVS 201
Score = 34.5 bits (69), Expect(4) = 2e-063
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2 / +1
Query: 488 DPCIFKHTHGRGIWELTKDDATFDALVNDGLASDSQLI 601
D +F T G G W+ + ++ ND +ASDS+LI
Sbjct: 436 DLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSKLI 549
Score = 32.7 bits (65), Expect(3) = 2e-025
Identities = 17/61 (27%), Positives = 28/61 (45%)
Frame = -2 / -3
Query: 270 RIRRRRLETLDGWRWTLLSIWDRLAAPPWRWMASAIRRCIADLSAMDFMNAKVVCQSSSC 91
R+RRR L D + + ++R PW W+ + A L M+ M A + + +C
Sbjct: 251 RMRRRTLPIFDDGT*RIETKFEREIGLPWFWIMFGMFISTAHLRDMESMKAYICLYNKAC 72
Query: 90 A 88
A
Sbjct: 71 A 69
Score = 31.3 bits (62), Expect(4) = 2e-063
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = +2 / +1
Query: 383 QSSQLAQTPLAAMVLDPTIVSPFSELGAWFQHE 481
+ S L P+ VLDPT+ + EL W E
Sbjct: 337 RGSDLCLAPMVECVLDPTLSGSYHELKKWIYEE 435
>emb|AX050436.1| Sequence 1 from Patent WO0071736
Length = 1231
Score = 196 bits (422), Expect(4) = 2e-063
Identities = 79/180 (43%), Positives = 118/180 (65%)
Frame = +2 / +1
Query: 599 IVDVAIKQSAEVFQGISSLVDVGGGIGTAAQAISKAFPHVKCSVLDLAHVVAKAPTHTDV 778
++++A++ VF G+ S+VDVGGG GT A+ I + FP +KC V D VV ++
Sbjct: 544 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 723
Query: 779 QFIAGDMFESIPPADAVLLKSVLHDWDHDDCVKILKNCKKAIPPREAGGKVIIINMVVGA 958
++ GDMF SIP ADAVLLK +LH+W DC++ILK CK+A+ GKV II+MV+
Sbjct: 724 TYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIDK 903
Query: 959 GPSDMKHKEMQAIFDVYIMFINGMERDEQEWSKIFSEAGYSDYRIIPVLGVRSIIEVYP* 1138
+ + +++ + DV + +NG ER+E+EW K+F EAG+ Y+I P+ G S+IE+YP*
Sbjct: 904 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1083
Score = 76.7 bits (161), Expect(3) = 2e-025
Identities = 50/116 (43%), Positives = 58/116 (50%)
Frame = -2 / -3
Query: 1137 YG*TSIIDRTPKTGIIL*SLYPASEKILLHSCSSRSMPLMNMI*TSNMACISLCFMSDGP 958
YG* SI D+ P G IL* PAS K HS S S P + TS + I + S
Sbjct: 1082 YG*ISIRDKNPVKGDIL*CWKPASMKSFFHSSSFLSFPFRQAMFTSIRSLI*VT*FSSFF 903
Query: 957 APTTMFIIITFPPASLGGIAFLQFFSIFTQSSWSQSCKTDFNSTASAGGMLSNMSP 790
TM II+TFP A L FFSI QS Q C+ + TASA G+ NMSP
Sbjct: 902 LSITMSIIVTFPLFPSFVTASLHFFSILRQSLSVQLCRIYLSKTASALGIDVNMSP 735
Score = 46.4 bits (95), Expect(3) = 2e-025
Identities = 20/49 (40%), Positives = 28/49 (57%)
Frame = -2 / -3
Query: 756 FATTWARSSTLHLTCGNAFEMAWAAVPMPPPTSTSELIPWKTSALCLMA 610
F+TT S+T+H G ++ A VP+PPPTST + P T + L A
Sbjct: 701 FSTTCGLSNTIHFNLGKVSQIILAVVPVPPPTSTIDSNPSNTKSQSLNA 555
Score = 41.8 bits (85), Expect(4) = 2e-063
Identities = 21/59 (35%), Positives = 31/59 (52%)
Frame = +2 / +1
Query: 44 MELSPNNSTDQSLLDAQLELWHTTFAFMKSMALKSAIHLRIADAIHLHGGAASLSQILS 220
M S N + AQ L+ +AF+ SM+LK A+ + I + I HG SLS ++S
Sbjct: 25 MASSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVS 201
Score = 34.5 bits (69), Expect(4) = 2e-063
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2 / +1
Query: 488 DPCIFKHTHGRGIWELTKDDATFDALVNDGLASDSQLI 601
D +F T G G W+ + ++ ND +ASDS+LI
Sbjct: 436 DLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSKLI 549
Score = 32.7 bits (65), Expect(3) = 2e-025
Identities = 17/61 (27%), Positives = 28/61 (45%)
Frame = -2 / -3
Query: 270 RIRRRRLETLDGWRWTLLSIWDRLAAPPWRWMASAIRRCIADLSAMDFMNAKVVCQSSSC 91
R+RRR L D + + ++R PW W+ + A L M+ M A + + +C
Sbjct: 251 RMRRRTLPIFDDGT*RIETKFEREIGLPWFWIMFGMFISTAHLRDMESMKAYICLYNKAC 72
Query: 90 A 88
A
Sbjct: 71 A 69
Score = 31.3 bits (62), Expect(4) = 2e-063
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = +2 / +1
Query: 383 QSSQLAQTPLAAMVLDPTIVSPFSELGAWFQHE 481
+ S L P+ VLDPT+ + EL W E
Sbjct: 337 RGSDLCLAPMVECVLDPTLSGSYHELKKWIYEE 435
>gb|AF000975.1|MSAF000975 Medicago sativa 7-O-methyltransferase (7-IOMT(6)) mRNA, complete cds
Length = 1284
Score = 196 bits (422), Expect(4) = 2e-063
Identities = 79/180 (43%), Positives = 118/180 (65%)
Frame = +2 / +3
Query: 599 IVDVAIKQSAEVFQGISSLVDVGGGIGTAAQAISKAFPHVKCSVLDLAHVVAKAPTHTDV 778
++++A++ VF G+ S+VDVGGG GT A+ I + FP +KC V D VV ++
Sbjct: 549 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 728
Query: 779 QFIAGDMFESIPPADAVLLKSVLHDWDHDDCVKILKNCKKAIPPREAGGKVIIINMVVGA 958
++ GDMF SIP ADAVLLK +LH+W DC++ILK CK+A+ GKV II+MV+
Sbjct: 729 TYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTIIDMVIDE 908
Query: 959 GPSDMKHKEMQAIFDVYIMFINGMERDEQEWSKIFSEAGYSDYRIIPVLGVRSIIEVYP* 1138
+ + +++ + DV + +NG ER+E+EW K+F EAG+ Y+I P+ G S+IE+YP*
Sbjct: 909 KKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1088
Score = 77.6 bits (163), Expect(3) = 9e-026
Identities = 50/116 (43%), Positives = 59/116 (50%)
Frame = -2 / -3
Query: 1137 YG*TSIIDRTPKTGIIL*SLYPASEKILLHSCSSRSMPLMNMI*TSNMACISLCFMSDGP 958
YG* SI D+ P G IL* PAS K HS S S P + TS + I + S
Sbjct: 1087 YG*ISIRDKNPVKGDIL*CWKPASMKSFFHSSSFLSFPFRQAMFTSIRSLI*VT*FSSFF 908
Query: 957 APTTMFIIITFPPASLGGIAFLQFFSIFTQSSWSQSCKTDFNSTASAGGMLSNMSP 790
+ TM II+TFP A L FFSI QS Q C+ + TASA G+ NMSP
Sbjct: 907 SSITMSIIVTFPLFPSFVTASLHFFSILRQSLSVQLCRIYLSKTASALGIDVNMSP 740
Score = 46.4 bits (95), Expect(3) = 9e-026
Identities = 20/49 (40%), Positives = 28/49 (57%)
Frame = -2 / -3
Query: 756 FATTWARSSTLHLTCGNAFEMAWAAVPMPPPTSTSELIPWKTSALCLMA 610
F+TT S+T+H G ++ A VP+PPPTST + P T + L A
Sbjct: 706 FSTTCGLSNTIHFNLGKVSQIILAVVPVPPPTSTIDSNPSNTKSQSLNA 560
Score = 41.8 bits (85), Expect(4) = 2e-063
Identities = 21/59 (35%), Positives = 31/59 (52%)
Frame = +2 / +3
Query: 44 MELSPNNSTDQSLLDAQLELWHTTFAFMKSMALKSAIHLRIADAIHLHGGAASLSQILS 220
M S N + AQ L+ +AF+ SM+LK A+ + I + I HG SLS ++S
Sbjct: 30 MASSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVS 206
Score = 34.5 bits (69), Expect(4) = 2e-063
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2 / +3
Query: 488 DPCIFKHTHGRGIWELTKDDATFDALVNDGLASDSQLI 601
D +F T G G W+ + ++ ND +ASDS+LI
Sbjct: 441 DLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSKLI 554
Score = 32.7 bits (65), Expect(3) = 9e-026
Identities = 17/61 (27%), Positives = 28/61 (45%)
Frame = -2 / -3
Query: 270 RIRRRRLETLDGWRWTLLSIWDRLAAPPWRWMASAIRRCIADLSAMDFMNAKVVCQSSSC 91
R+RRR L D + + ++R PW W+ + A L M+ M A + + +C
Sbjct: 256 RMRRRTLPIFDDGT*RIETKFEREIGLPWFWIMFGMFISTAHLRDMESMKAYICLYNKAC 77
Query: 90 A 88
A
Sbjct: 76 A 74
Score = 31.3 bits (62), Expect(4) = 2e-063
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = +2 / +3
Query: 383 QSSQLAQTPLAAMVLDPTIVSPFSELGAWFQHE 481
+ S L P+ VLDPT+ + EL W E
Sbjct: 342 RGSDLCLAPMVECVLDPTLSGSYHELKKWIYEE 440
>gb|AF023481.1|AF023481 Medicago sativa o-methytransferase (iomt2003) mRNA, complete cds
Length = 1194
Score = 99.1 bits (210), Expect(5) = 4e-056
Identities = 39/91 (42%), Positives = 61/91 (67%)
Frame = +2 / +1
Query: 866 DCVKILKNCKKAIPPREAGGKVIIINMVVGAGPSDMKHKEMQAIFDVYIMFINGMERDEQ 1045
DC++ILK CK+A+ GKV II+MV+ + + +++ + DV + +NG ER+E+
Sbjct: 787 DCLRILKKCKEAVTNDGKRGKVTIIDMVIDEKKDENQVTQIKLLMDVNMACLNGKERNEE 966
Query: 1046 EWSKIFSEAGYSDYRIIPVLGVRSIIEVYP* 1138
EW K+F EAG+ Y+I P+ G S+IE+YP*
Sbjct: 967 EWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1059
Score = 91.3 bits (193), Expect(5) = 4e-056
Identities = 38/83 (45%), Positives = 53/83 (63%)
Frame = +2 / +1
Query: 599 IVDVAIKQSAEVFQGISSLVDVGGGIGTAAQAISKAFPHVKCSVLDLAHVVAKAPTHTDV 778
++++A++ VF G+ S+VDVGGG GT A+ I + FP +KC V D VV ++
Sbjct: 547 LINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNL 726
Query: 779 QFIAGDMFESIPPADAVLLKSVL 847
++ GDMF SIP ADAVLLK L
Sbjct: 727 TYVGGDMFTSIPNADAVLLKDCL 795
Score = 41.8 bits (85), Expect(5) = 4e-056
Identities = 21/59 (35%), Positives = 31/59 (52%)
Frame = +2 / +1
Query: 44 MELSPNNSTDQSLLDAQLELWHTTFAFMKSMALKSAIHLRIADAIHLHGGAASLSQILS 220
M S N + AQ L+ +AF+ SM+LK A+ + I + I HG SLS ++S
Sbjct: 28 MASSINGRKPSEIFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVS 204
Score = 34.5 bits (69), Expect(5) = 4e-056
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2 / +1
Query: 488 DPCIFKHTHGRGIWELTKDDATFDALVNDGLASDSQLI 601
D +F T G G W+ + ++ ND +ASDS+LI
Sbjct: 439 DLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSKLI 552
Score = 31.3 bits (62), Expect(5) = 4e-056
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = +2 / +1
Query: 383 QSSQLAQTPLAAMVLDPTIVSPFSELGAWFQHE 481
+ S L P+ VLDPT+ + EL W E
Sbjct: 340 RGSDLCLAPMVECVLDPTLSGSYHELKKWIYEE 438
>gb|M63853.1|ALFSALMCAT Medicago sativa S-adenosyl-L-methionine: caffeic acid
3-0-methyltransferase mRNA, complete cds
Length = 1317
Score = 117 bits (251), Expect = 2e-027
Identities = 48/108 (44%), Positives = 65/108 (60%)
Frame = +2 / +1
Query: 584 SDSQLIVDVAIKQSAEVFQGISSLVDVGGGIGTAAQAISKAFPHVKCSVLDLAHVVAKAP 763
SD I I ++ F+G+ SLVDVGGG G I +P +K DL HV+ AP
Sbjct: 571 SDHSTITMKKILETYTGFEGLKSLVDVGGGTGAVINTIVSKYPTIKGINFDLPHVIEDAP 750
Query: 764 THTDVQFIAGDMFESIPPADAVLLKSVLHDWDHDDCVKILKNCKKAIP 907
++ V+ + GDMF SIP ADAV +K + HDW + C+K LKNC +A+P
Sbjct: 751 SYPGVEHVGGDMFVSIPKADAVFMKWICHDWSDEHCLKFLKNCYEALP 894
>emb|AX259373.1| Sequence 3 from Patent WO0173090
Length = 1098
Score = 117 bits (251), Expect = 2e-027
Identities = 48/108 (44%), Positives = 65/108 (60%)
Frame = +2 / +1
Query: 584 SDSQLIVDVAIKQSAEVFQGISSLVDVGGGIGTAAQAISKAFPHVKCSVLDLAHVVAKAP 763
SD I I ++ F+G+ SLVDVGGG G I +P +K DL HV+ AP
Sbjct: 541 SDHSTITMKKILETYTGFEGLKSLVDVGGGTGAVINTIVSKYPTIKGINFDLPHVIEDAP 720
Query: 764 THTDVQFIAGDMFESIPPADAVLLKSVLHDWDHDDCVKILKNCKKAIP 907
++ V+ + GDMF SIP ADAV +K + HDW + C+K LKNC +A+P
Sbjct: 721 SYPGVEHVGGDMFVSIPKADAVFMKWICHDWSDEHCLKFLKNCYEALP 864
>gb|L10211.1|ALFIOM Medicago sativa isoliquiritigenin 2'-O-methyltransferase mRNA,
complete cds
Length = 1300
Score = 106 bits (226), Expect = 7e-024
Identities = 40/92 (43%), Positives = 59/92 (64%)
Frame = +2 / +3
Query: 635 FQGISSLVDVGGGIGTAAQAISKAFPHVKCSVLDLAHVVAKAPTHTDVQFIAGDMFESIP 814
F+GIS+LVDVGGG G + I +P +K DL V+ AP + ++ + GDMF S+P
Sbjct: 648 FEGISTLVDVGGGSGRNLELIISKYPLIKGINFDLPQVIENAPPLSGIEHVGGDMFASVP 827
Query: 815 PADAVLLKSVLHDWDHDDCVKILKNCKKAIPP 910
DA++LK+V H+W + C++ L NC KA+ P
Sbjct: 828 QGDAMILKAVCHNWSDEKCIEFLSNCHKALSP 923
>gb|CO512262.1|CO512262 s13dSG03H0600048_114038 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 588
Score = 105 bits (224), Expect = 1e-023
Identities = 40/86 (46%), Positives = 55/86 (63%)
Frame = +2 / +1
Query: 635 FQGISSLVDVGGGIGTAAQAISKAFPHVKCSVLDLAHVVAKAPTHTDVQFIAGDMFESIP 814
F I LVDVGGG G I+ +P+++ DL V+ APT+ V + GDMFE IP
Sbjct: 328 FDHIKKLVDVGGGNGINLNLITSKYPYIQGVNFDLPQVIEHAPTYPGVTHVGGDMFEGIP 507
Query: 815 PADAVLLKSVLHDWDHDDCVKILKNC 892
DA+ LK++LH+WD + C+K+LKNC
Sbjct: 508 KGDAIFLKTILHNWDDEHCLKLLKNC 585
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,168,927
Number of Sequences: 7669
Number of extensions: 184344
Number of successful extensions: 1282
Number of sequences better than 1.0e-020: 9
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 373564120
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)