TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419471.2.1
(556 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514605.1|CO514605 s13dSG42G0700056_327870 Glandular tr... 269 2e-073
gb|AY560003.1| Medicago sativa S-adenosylmethionine synthas... 264 5e-072
gb|CO516632.1|CO516632 s13dSG66E0200007_446592 Glandular tr... 229 2e-061
gb|CO514747.1|CO514747 s13dSG55F1100095_328154 Glandular tr... 228 5e-061
>gb|CO514605.1|CO514605 s13dSG42G0700056_327870 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 600
Score = 269 bits (582), Expect = 2e-073
Identities = 108/119 (90%), Positives = 115/119 (96%)
Frame = +1 / +2
Query: 199 DTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKA 378
+TFL+TSESVNEGHPDKLCDQ+SDAVLDACL +DPDSKVACETCTKTNMVMVFGEITTKA
Sbjct: 122 ETFLYTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKA 301
Query: 379 NVDYEKIVRETCRNIGFVSNDVGLDADHCKVLGNIEQQSPDIAQGVHGHFTKRPEEIGA 555
NVDYEKIVR TCRNIGFVS+DVGLDAD+CKVL NIEQQSPDIAQGVHGH T+RPEEIGA
Sbjct: 302 NVDYEKIVRNTCRNIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTERPEEIGA 478
Score = 199 bits (428), Expect = 3e-052
Identities = 94/127 (74%), Positives = 99/127 (77%)
Frame = -3 / -1
Query: 554 APISSGRLVKCPCTP*AISGDCCSMFPSTLQWSASSPTSFDTKPMLRHVSLTIFS*STLA 375
APISSG V+ P TP*AISG CCSM TLQ SAS PTS +TKPM HV TIFS*ST A
Sbjct: 477 APISSGLSVRWP*TP*AISGLCCSMLTRTLQLSASRPTSSETKPMFLHVLRTIFS*STFA 298
Query: 374 LVVISPKTMTMLVLVQVSQATLLSGSSARQASRTASETWSQSLSGCPSFTDSEVKRKVSS 195
VVISPKT+TMLV VQVS ATLLSGS +RQASRTASE WSQSLSGCPSFTDS+V RKVS
Sbjct: 297 FVVISPKTITMLVFVQVSHATLLSGSCSRQASRTASEIWSQSLSGCPSFTDSDVYRKVSV 118
Query: 194 PAIFSDL 174
DL
Sbjct: 117 AIFNQDL 97
Score = 120 bits (256), Expect = 2e-028
Identities = 55/118 (46%), Positives = 74/118 (62%)
Frame = +3 / +1
Query: 198 RHLPLHLGVRERGTP*QALRPGLRCCSGRLPC*GP*QQGCLRDLHQDQHGHGLW*DHHQG 377
R+ P+H+ + ERGTP QALRP L C S LP QQ C+ +LH+DQHG+GLW*DH++
Sbjct: 121 RNFPIHI*ICERGTPRQALRPDL*CRS*CLPRARSRQQSCM*NLHKDQHGYGLW*DHNKS 300
Query: 378 QCRLREDCQGDMPQHWFRVERCRA*R*PLQGAWEH*AAVP*YCSGCARALHQAPRGDW 551
+C L +DC M +H F RC +* * LQ +H* *YCS C+ ++ G+W
Sbjct: 301 KC*L*KDCP*HMQEHRFCFRRCWS*C*QLQSPCQH*TTES*YCSRCSWPSYRKT*GNW 474
Score = 113 bits (241), Expect = 2e-026
Identities = 57/113 (50%), Positives = 74/113 (65%)
Frame = -2 / -3
Query: 555 SSNLLGALGEVPVHTLSNIRGLLLNVPKHLAVVSVKPDIVRHETNVAACLPDNXXXXXXX 376
S+N L + ++ ++TLSNIR LL NV K AVVS+K +IV ++T V AC+ DN
Sbjct: 478 STNFLRSFCKMAMNTLSNIRTLLFNVDKDFAVVSIKTNIV*NKTYVPACVTDNLFIVNIC 299
Query: 375 XXXXLTKDHDHVGLGAGLASNLAVRVLSKASVQNSI*DLVAELVRVSLVHGLR 217
LTKDH+HVGL A N AV +L +AS++N I DLVA+LV VSLVH R
Sbjct: 298 FCCDLTKDHNHVGLCASFTCNFAVWILLEASIKNGIRDLVAKLVGVSLVHRFR 140
>gb|AY560003.1| Medicago sativa S-adenosylmethionine synthase mRNA, complete cds
Length = 1173
Score = 264 bits (572), Expect = 5e-072
Identities = 107/120 (89%), Positives = 114/120 (95%)
Frame = +1 / +1
Query: 196 LDTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTK 375
++TFLFTSESVNEGHPDKLCDQ+SDAVLDACL +D DSKVACETCTKTNMVMVFGEITTK
Sbjct: 1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDVDSKVACETCTKTNMVMVFGEITTK 180
Query: 376 ANVDYEKIVRETCRNIGFVSNDVGLDADHCKVLGNIEQQSPDIAQGVHGHFTKRPEEIGA 555
A VDYEKIVR+TCR IGFVS+DVGLDAD+CKVL NIEQQSPDIAQGVHGH TKRPEEIGA
Sbjct: 181 AKVDYEKIVRDTCRKIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIGA 360
Score = 187 bits (402), Expect = 1e-048
Identities = 91/119 (76%), Positives = 96/119 (80%)
Frame = -3 / -2
Query: 554 APISSGRLVKCPCTP*AISGDCCSMFPSTLQWSASSPTSFDTKPMLRHVSLTIFS*STLA 375
APISSG V+ PCTP*AISG CCSM TLQ SAS PTS +T P+ VS TIFS*STLA
Sbjct: 359 APISSGLFVR*PCTP*AISGLCCSMLTRTLQLSASRPTSSETNPIFLQVSRTIFS*STLA 180
Query: 374 LVVISPKTMTMLVLVQVSQATLLSGSSARQASRTASETWSQSLSGCPSFTDSEVKRKVS 198
LVVISPKT+TMLVLVQVS ATLLS S +RQAS TASE SQSLSG PSFTDSEV RKVS
Sbjct: 179 LVVISPKTITMLVLVQVSHATLLSTSCSRQASSTASEI*SQSLSG*PSFTDSEVNRKVS 3
Score = 125 bits (268), Expect = 4e-030
Identities = 60/119 (50%), Positives = 84/119 (70%)
Frame = -2 / -1
Query: 555 SSNLLGALGEVPVHTLSNIRGLLLNVPKHLAVVSVKPDIVRHETNVAACLPDNXXXXXXX 376
S+NLL + ++ ++TLSNIR LLLNV K+LAVVS+K I+R++ N++ + N
Sbjct: 360 STNLLRSFCKMTMYTLSNIRTLLLNVDKNLAVVSIKTYIIRNKPNLSTGITHNFLIVNLS 181
Query: 375 XXXXLTKDHDHVGLGAGLASNLAVRVLSKASVQNSI*DLVAELVRVSLVHGLRGEEEGV 199
LTKDH+HVGL A L NLAV VL KAS+ +SI DL+ +LV ++L+HGLRGE +G+
Sbjct: 180 LGCDLTKDHNHVGLSASLTCNLAVYVLFKASI*HSIRDLITKLVGMTLIHGLRGE*KGL 4
Score = 116 bits (249), Expect = 2e-027
Identities = 59/116 (50%), Positives = 74/116 (63%)
Frame = +3 / +3
Query: 204 LPLHLGVRERGTP*QALRPGLRCCSGRLPC*GP*QQGCLRDLHQDQHGHGLW*DHHQGQC 383
L +HL VRE G+ QAL L CC+ LP G QQGC+ DLH DQHG+GLW*DH+QG
Sbjct: 9 LSIHL*VRE*GSSRQAL*SNL*CCARCLP*TGRRQQGCM*DLH*DQHGYGLW*DHNQG*G 188
Query: 384 RLREDCQGDMPQHWFRVERCRA*R*PLQGAWEH*AAVP*YCSGCARALHQAPRGDW 551
L E+C + + W CR+* * LQG+ +H*AA *YCSGC + ++ GDW
Sbjct: 189 *L*ENCA*YL*KDWVCF**CRS*C*QLQGSCQH*AAES*YCSGCTWSSYKKT*GDW 356
>gb|CO516632.1|CO516632 s13dSG66E0200007_446592 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 425
Score = 229 bits (495), Expect = 2e-061
Identities = 92/101 (91%), Positives = 98/101 (97%)
Frame = +1 / +2
Query: 199 DTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKA 378
+TFL+TSESVNEGHPDKLCDQ+SDAVLDACL +DPDSKVACETCTKTNMVMVFGEITTKA
Sbjct: 122 ETFLYTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKA 301
Query: 379 NVDYEKIVRETCRNIGFVSNDVGLDADHCKVLGNIEQQSPD 501
NVDYEKIVR TCRNIGFVS+DVGLDAD+CKVL NIEQQSPD
Sbjct: 302 NVDYEKIVRNTCRNIGFVSDDVGLDADNCKVLVNIEQQSPD 424
Score = 174 bits (375), Expect = 7e-045
Identities = 81/109 (74%), Positives = 85/109 (77%)
Frame = -3 / -3
Query: 500 SGDCCSMFPSTLQWSASSPTSFDTKPMLRHVSLTIFS*STLALVVISPKTMTMLVLVQVS 321
SG CCSM TLQ SAS PTS +TKPM HV TIFS*ST A VVISPKT+TMLV VQVS
Sbjct: 423 SGLCCSMLTRTLQLSASRPTSSETKPMFLHVLRTIFS*STFAFVVISPKTITMLVFVQVS 244
Query: 320 QATLLSGSSARQASRTASETWSQSLSGCPSFTDSEVKRKVSSPAIFSDL 174
ATLLSGS +RQASRTASE WSQSLSGCPSFTDS+V RKVS DL
Sbjct: 243 HATLLSGSCSRQASRTASEIWSQSLSGCPSFTDSDVYRKVSVAIFNQDL 97
Score = 104 bits (221), Expect = 1e-023
Identities = 48/96 (50%), Positives = 63/96 (65%)
Frame = +3 / +1
Query: 198 RHLPLHLGVRERGTP*QALRPGLRCCSGRLPC*GP*QQGCLRDLHQDQHGHGLW*DHHQG 377
R+ P+H+ + ERGTP QALRP L C S LP QQ C+ +LH+DQHG+GLW*DH++
Sbjct: 121 RNFPIHI*ICERGTPRQALRPDL*CRS*CLPRARSRQQSCM*NLHKDQHGYGLW*DHNKS 300
Query: 378 QCRLREDCQGDMPQHWFRVERCRA*R*PLQGAWEH* 485
+C L +DC M +H F RC +* * LQ +H*
Sbjct: 301 KC*L*KDCP*HMQEHRFCFRRCWS*C*QLQSPCQH* 408
Score = 98.2 bits (208), Expect = 7e-022
Identities = 50/95 (52%), Positives = 61/95 (64%)
Frame = -2 / -2
Query: 501 IRGLLLNVPKHLAVVSVKPDIVRHETNVAACLPDNXXXXXXXXXXXLTKDHDHVGLGAGL 322
IR LL NV K AVVS+K +IV ++T V AC+ DN LTKDH+HVGL A
Sbjct: 424 IRTLLFNVDKDFAVVSIKTNIV*NKTYVPACVTDNLFIVNICFCCDLTKDHNHVGLCASF 245
Query: 321 ASNLAVRVLSKASVQNSI*DLVAELVRVSLVHGLR 217
N AV +L +AS++N I DLVA+LV VSLVH R
Sbjct: 244 TCNFAVWILLEASIKNGIRDLVAKLVGVSLVHRFR 140
>gb|CO514747.1|CO514747 s13dSG55F1100095_328154 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 435
Score = 228 bits (492), Expect = 5e-061
Identities = 92/103 (89%), Positives = 98/103 (95%)
Frame = +1 / +1
Query: 199 DTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKA 378
+TFLFTSESVNEGHPDKLCDQ+SDAVLDACL +DPDSK ACETCTKTNMVMVFGEITTKA
Sbjct: 127 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKGACETCTKTNMVMVFGEITTKA 306
Query: 379 NVDYEKIVRETCRNIGFVSNDVGLDADHCKVLGNIEQQSPDIA 507
NVDYEKIVR+TCR IGF+S+DVGLDAD CKVL NIEQQSPDIA
Sbjct: 307 NVDYEKIVRDTCRTIGFISDDVGLDADKCKVLVNIEQQSPDIA 435
Score = 171 bits (368), Expect = 6e-044
Identities = 80/104 (76%), Positives = 85/104 (81%)
Frame = -3 / -2
Query: 506 AISGDCCSMFPSTLQWSASSPTSFDTKPMLRHVSLTIFS*STLALVVISPKTMTMLVLVQ 327
AISG CCSM TL SAS PTS + PM+RHVS TIFS*STLALVVISPKTMTMLVLV
Sbjct: 434 AISGLCCSMLTKTLHLSASRPTSSEMNPMVRHVSRTIFS*STLALVVISPKTMTMLVLVH 255
Query: 326 VSQATLLSGSSARQASRTASETWSQSLSGCPSFTDSEVKRKVSS 195
VSQA LLSGS +RQAS TASE WS SLSG PSFTDS+V RKVS+
Sbjct: 254 VSQAPLLSGSCSRQASSTASEIWSHSLSG*PSFTDSDVNRKVSA 123
Score = 116 bits (248), Expect = 2e-027
Identities = 53/102 (51%), Positives = 69/102 (67%)
Frame = +3 / +3
Query: 201 HLPLHLGVRERGTP*QALRPGLRCCSGRLPC*GP*QQGCLRDLHQDQHGHGLW*DHHQGQ 380
+L +++ + ERG+P QA+RP L C + RL GP*QQGCL D+HQD HGHGLW +++QGQ
Sbjct: 129 NLSIYI*ISERGSPRQAVRPDL*CSARRLS*AGP*QQGCL*DMHQD*HGHGLWRNNNQGQ 308
Query: 381 CRLREDCQGDMPQHWFRVERCRA*R*PLQGAWEH*AAVP*YC 506
RL ED MP HW + C +* +QG +H*AA *YC
Sbjct: 309 RRL*EDRS*HMPHHWIHL**CWS*CRQMQGLGQH*AAES*YC 434
Score = 105 bits (223), Expect = 6e-024
Identities = 52/102 (50%), Positives = 67/102 (65%)
Frame = -2 / -1
Query: 507 SNIRGLLLNVPKHLAVVSVKPDIVRHETNVAACLPDNXXXXXXXXXXXLTKDHDHVGLGA 328
SNIR LLLNV + LA V +K +I+R E+N AAC+ ++ +KDHDHV LGA
Sbjct: 435 SNIRTLLLNVDQDLAFVGIKTNIIRDESNGAACVTNDLLIVYVGLGCYFSKDHDHVSLGA 256
Query: 327 GLASNLAVRVLSKASVQNSI*DLVAELVRVSLVHGLRGEEEG 202
L LAVRVL K SV++ I DLVA+LV V+LVH R + +G
Sbjct: 255 CLTGTLAVRVLLKTSVEHCIRDLVAQLVGVTLVH*FRCK*KG 130
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 3,936,037
Number of Sequences: 7669
Number of extensions: 43653
Number of successful extensions: 1044
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 41
effective length of database: 934,139
effective search space used: 133581877
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)