TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2418879.2.3
(1694 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY560003.1| Medicago sativa S-adenosylmethionine synthas... 774 0.0
gb|CO514605.1|CO514605 s13dSG42G0700056_327870 Glandular tr... 366 e-102
gb|CO514483.1|CO514483 s13dSG43C0600040_327626 Glandular tr... 188 3e-065
gb|CO514747.1|CO514747 s13dSG55F1100095_328154 Glandular tr... 234 2e-062
gb|CO516632.1|CO516632 s13dSG66E0200007_446592 Glandular tr... 232 7e-062
gb|AW698879.1|AW698879 r109 non-glandular-haired subtracted... 111 3e-025
>gb|AY560003.1| Medicago sativa S-adenosylmethionine synthase mRNA, complete cds
Length = 1173
Score = 774 bits (1684), Expect(2) = 0.0
Identities = 322/358 (89%), Positives = 332/358 (92%)
Frame = -2 / +1
Query: 1606 ESFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKA 1427
E+FLFTSESVNEGHPDKLCDQ+SDAVLDACL QD DSKVACETCTKTNMVMVFGEITTKA
Sbjct: 4 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDVDSKVACETCTKTNMVMVFGEITTKA 183
Query: 1426 TVDYEKIVRDTCREIGFTSDDVGLDADRCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAG 1247
VDYEKIVRDTCR+IGF SDDVGLDAD CKVLVNIEQQSPDIAQGVHGH TKRPEEIGAG
Sbjct: 184 KVDYEKIVRDTCRKIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIGAG 363
Query: 1246 DQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYVNEGG 1067
DQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTC WLRPDGKTQVTVEY N+ G
Sbjct: 364 DQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCPWLRPDGKTQVTVEYYNDNG 543
Query: 1066 AMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLDEKTIFHLNPSGRFVIG 887
AMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLD KTIFHLNPSGRFVIG
Sbjct: 544 AMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLDSKTIFHLNPSGRFVIG 723
Query: 886 GPHGDAGLTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYVARQAAKSIVASGLARR 707
GPHGDAGLTGRKIIIDTY GKDPTKVDRSGAY+ RQAAKSIVASGLARR
Sbjct: 724 GPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARR 903
Query: 706 CLVQVSYAIGVPEPLSVFVNSYGTGTIPDKEILKIVKENFDFRPGMISINLDLKKGGN 533
C+VQVSYAIGVPEPLSVFV++YGTG IPDKEIL IVK+NFDFRPGMISINLDL +GGN
Sbjct: 904 CIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILNIVKQNFDFRPGMISINLDLLRGGN 1077
Score = 449 bits (974), Expect(3) = e-158
Identities = 207/257 (80%), Positives = 218/257 (84%)
Frame = +3 / -2
Query: 837 VSMMILRPVRPASPWGPPMTKRPDGLRWKMVFSSRYFSGITGLMTCSLRSAAISSLVTVS 1016
VSMMI PVRPASP GPPMTKRP+G + KMV SRYFSGITG +TCSLRSAAISSLVTVS
Sbjct: 773 VSMMIFLPVRPASP*GPPMTKRPEGFK*KMVLESRYFSGITGFITCSLRSAAISSLVTVS 594
Query: 1017 SCWVEMSTVCTRTGTMAPPSFTYSTVTWVLPSGLSQAQVPFLRTSVRRAPSLVASTWLSG 1196
SC VEMSTV TRTGTMAP S YSTVT VLPSGL+Q VPFLRTSVRRAPSLVA TWL G
Sbjct: 593 SC*VEMSTVWTRTGTMAPLSL*YSTVT*VLPSGLNQGHVPFLRTSVRRAPSLVARTWLKG 414
Query: 1197 ISSGVSSVAYPNMWPWSPAPISSGRFVKCPCTPCAMSGDCCSMFTSTLQRSASRPTSSEV 1376
I+SGVSSVA PNM PWSPAPISSG FV+ PCTP A+SG CCSM T TLQ SASRPTSSE
Sbjct: 413 INSGVSSVA*PNM*PWSPAPISSGLFVR*PCTP*AISGLCCSMLTRTLQLSASRPTSSET 234
Query: 1377 NPISRQVSRTIFS*STVALVVISPNTITMLVLVQVSQATLLSGSCARHASSTASDTWSHS 1556
NPI QVSRTIFS*ST+ALVVISP TITMLVLVQVS ATLLS SC+R ASSTAS+ S S
Sbjct: 233 NPIFLQVSRTIFS*STLALVVISPKTITMLVLVQVSHATLLSTSCSRQASSTASEI*SQS 54
Query: 1557 LSGCPSFTDSEVKRKLS 1607
LSG PSFTDSEV RK+S
Sbjct: 53 LSG*PSFTDSEVNRKVS 3
Score = 101 bits (216), Expect(3) = e-158
Identities = 43/56 (76%), Positives = 48/56 (85%)
Frame = +3 / -2
Query: 534 LPPFFRSRLMLIIPGLKSKFSFTILRISLSGIVPVPYELTNTDRGSGTPMAYDTCT 701
LPP RSRL+ I+PGLKSKF FTILRISLSGI PVPY TNTD+GSGTP+A +TCT
Sbjct: 1076 LPPLKRSRLIEIMPGLKSKFCFTILRISLSGIFPVPYVSTNTDKGSGTPIA*ETCT 909
Score = 85.8 bits (181), Expect(2) = 6e-024
Identities = 40/119 (33%), Positives = 66/119 (55%)
Frame = +2 / -1
Query: 1085 LHRHLGLAVGPQPGAGAVLAHLGEARAQLGGQHVAQRHQLGGLVGGVPEHVALVARADLL 1264
L+ L L + QP A +LA+L E +LG +++AQRHQ L+ + +HV LV +LL
Sbjct: 525 LNSDLSLTIRSQPRACTILANLSETSTKLGCKNMAQRHQFRSLISSIAKHVTLVTSTNLL 346
Query: 1265 GPLREVPVHALRDVGGLLLDVHQHLXXXXXXXXXXXXXPDLAAGIAHDLLVVHGRLGRD 1441
++ ++ L ++ LLL+V ++L P+L+ GI H+ L+V+ LG D
Sbjct: 345 RSFCKMTMYTLSNIRTLLLNVDKNLAVVSIKTYIIRNKPNLSTGITHNFLIVNLSLGCD 169
Score = 78.5 bits (165), Expect(2) = 0.0
Identities = 29/37 (78%), Positives = 34/37 (91%)
Frame = -2 / +1
Query: 550 LKKGGNRFIKTAAYGHFGRDDADFTWEVVKPLKFDKA 440
L+ G RF+KTAAYGHFGR+DADFTWEVVKPLK++KA
Sbjct: 1063 LRGGNGRFLKTAAYGHFGREDADFTWEVVKPLKWEKA 1173
Score = 51.9 bits (107), Expect(3) = e-158
Identities = 25/34 (73%), Positives = 26/34 (76%)
Frame = +3 / -2
Query: 441 ALSNLRGFTTSQVKSASSRPKWP*AAVLMNLLPP 542
A S+LRGFTTS VKSASS PK P*AAV NL P
Sbjct: 1172 AFSHLRGFTTSHVKSASSLPKCP*AAVFKNLPLP 1071
Score = 40.5 bits (82), Expect(2) = 6e-024
Identities = 15/31 (48%), Positives = 23/31 (74%)
Frame = +2 / -1
Query: 1514 EACVKHGVRHLVAQLVGVPLVHGLRGEKEAL 1606
+A + H +R L+ +LVG+ L+HGLRGE + L
Sbjct: 96 KASI*HSIRDLITKLVGMTLIHGLRGE*KGL 4
>gb|CO514605.1|CO514605 s13dSG42G0700056_327870 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 600
Score = 366 bits (793), Expect = e-102
Identities = 148/162 (91%), Positives = 155/162 (95%)
Frame = -2 / +2
Query: 1615 MAAESFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEIT 1436
MA E+FL+TSESVNEGHPDKLCDQ+SDAVLDACL QDPDSKVACETCTKTNMVMVFGEIT
Sbjct: 113 MATETFLYTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEIT 292
Query: 1435 TKATVDYEKIVRDTCREIGFTSDDVGLDADRCKVLVNIEQQSPDIAQGVHGHFTKRPEEI 1256
TKA VDYEKIVR+TCR IGF SDDVGLDAD CKVLVNIEQQSPDIAQGVHGH T+RPEEI
Sbjct: 293 TKANVDYEKIVRNTCRNIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTERPEEI 472
Query: 1255 GAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGT 1130
GAGDQGHMFGYATDETPELMPL+HVLATKLGA+LTEVRKNGT
Sbjct: 473 GAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGT 598
Score = 263 bits (569), Expect = 4e-071
Identities = 121/162 (74%), Positives = 130/162 (80%)
Frame = +3 / -1
Query: 1131 VPFLRTSVRRAPSLVASTWLSGISSGVSSVAYPNMWPWSPAPISSGRFVKCPCTPCAMSG 1310
VPFLRTSV AP+LVA TW+ GISSGVSSVAYPNM P SPAPISSG V+ P TP A+SG
Sbjct: 597 VPFLRTSVSLAPNLVARTWVKGISSGVSSVAYPNM*P*SPAPISSGLSVRWP*TP*AISG 418
Query: 1311 DCCSMFTSTLQRSASRPTSSEVNPISRQVSRTIFS*STVALVVISPNTITMLVLVQVSQA 1490
CCSM T TLQ SASRPTSSE P+ V RTIFS*ST A VVISP TITMLV VQVS A
Sbjct: 417 LCCSMLTRTLQLSASRPTSSETKPMFLHVLRTIFS*STFAFVVISPKTITMLVFVQVSHA 238
Query: 1491 TLLSGSCARHASSTASDTWSHSLSGCPSFTDSEVKRKLSAAI 1616
TLLSGSC+R AS TAS+ WS SLSGCPSFTDS+V RK+S AI
Sbjct: 237 TLLSGSCSRQASRTASEIWSQSLSGCPSFTDSDVYRKVSVAI 112
>gb|CO514483.1|CO514483 s13dSG43C0600040_327626 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 505
Score = 188 bits (405), Expect(2) = 3e-065
Identities = 78/87 (89%), Positives = 84/87 (96%)
Frame = -2 / +3
Query: 793 KDPTKVDRSGAYVARQAAKSIVASGLARRCLVQVSYAIGVPEPLSVFVNSYGTGTIPDKE 614
KDPTKVDRSGAY+ RQAAKSIVA+GLARRC+VQVSYAIGVPEPLSVFV+SYGTG IPDKE
Sbjct: 24 KDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKE 203
Query: 613 ILKIVKENFDFRPGMISINLDLKKGGN 533
IL IVKE+FDFRPGMISINLDLK+GGN
Sbjct: 204 ILNIVKESFDFRPGMISINLDLKRGGN 284
Score = 92.7 bits (196), Expect(2) = 7e-028
Identities = 42/57 (73%), Positives = 46/57 (80%)
Frame = +3 / -1
Query: 531 LLPPFFRSRLMLIIPGLKSKFSFTILRISLSGIVPVPYELTNTDRGSGTPMAYDTCT 701
L PP RSRL+ IIPGLKSK SFT+LRISLSGI PVP E TNTD GSGTP+A +T T
Sbjct: 286 LFPPLLRSRLIEIIPGLKSKDSFTMLRISLSGIFPVP*ESTNTDNGSGTPIA*ET*T 116
Score = 76.2 bits (160), Expect(2) = 3e-065
Identities = 29/33 (87%), Positives = 30/33 (90%)
Frame = -2 / +3
Query: 541 GGNRFIKTAAYGHFGRDDADFTWEVVKPLKFDK 443
G NRF+KTAAYGHFGRDDADFTWEVVKPLK K
Sbjct: 279 GNNRFLKTAAYGHFGRDDADFTWEVVKPLKGGK 377
Score = 46.9 bits (96), Expect(2) = 7e-028
Identities = 23/30 (76%), Positives = 23/30 (76%)
Frame = +3 / -1
Query: 453 LRGFTTSQVKSASSRPKWP*AAVLMNLLPP 542
L GFTTS VKSASS PK P*AAV NLL P
Sbjct: 367 LSGFTTSHVKSASSLPK*P*AAVFKNLLFP 278
>gb|CO514747.1|CO514747 s13dSG55F1100095_328154 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 435
Score = 234 bits (506), Expect = 2e-062
Identities = 96/104 (92%), Positives = 99/104 (95%)
Frame = -2 / +1
Query: 1609 AESFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTK 1430
AE+FLFTSESVNEGHPDKLCDQ+SDAVLDACL QDPDSK ACETCTKTNMVMVFGEITTK
Sbjct: 124 AETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKGACETCTKTNMVMVFGEITTK 303
Query: 1429 ATVDYEKIVRDTCREIGFTSDDVGLDADRCKVLVNIEQQSPDIA 1298
A VDYEKIVRDTCR IGF SDDVGLDAD+CKVLVNIEQQSPDIA
Sbjct: 304 ANVDYEKIVRDTCRTIGFISDDVGLDADKCKVLVNIEQQSPDIA 435
Score = 182 bits (391), Expect = 1e-046
Identities = 81/106 (76%), Positives = 91/106 (85%)
Frame = +3 / -2
Query: 1299 AMSGDCCSMFTSTLQRSASRPTSSEVNPISRQVSRTIFS*STVALVVISPNTITMLVLVQ 1478
A+SG CCSM T TL SASRPTSSE+NP+ R VSRTIFS*ST+ALVVISP T+TMLVLV
Sbjct: 434 AISGLCCSMLTKTLHLSASRPTSSEMNPMVRHVSRTIFS*STLALVVISPKTMTMLVLVH 255
Query: 1479 VSQATLLSGSCARHASSTASDTWSHSLSGCPSFTDSEVKRKLSAAI 1616
VSQA LLSGSC+R ASSTAS+ WSHSLSG PSFTDS+V RK+SA +
Sbjct: 254 VSQAPLLSGSCSRQASSTASEIWSHSLSG*PSFTDSDVNRKVSAIV 117
>gb|CO516632.1|CO516632 s13dSG66E0200007_446592 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 425
Score = 232 bits (502), Expect = 7e-062
Identities = 94/104 (90%), Positives = 98/104 (94%)
Frame = -2 / +2
Query: 1615 MAAESFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEIT 1436
MA E+FL+TSESVNEGHPDKLCDQ+SDAVLDACL QDPDSKVACETCTKTNMVMVFGEIT
Sbjct: 113 MATETFLYTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEIT 292
Query: 1435 TKATVDYEKIVRDTCREIGFTSDDVGLDADRCKVLVNIEQQSPD 1304
TKA VDYEKIVR+TCR IGF SDDVGLDAD CKVLVNIEQQSPD
Sbjct: 293 TKANVDYEKIVRNTCRNIGFVSDDVGLDADNCKVLVNIEQQSPD 424
Score = 171 bits (369), Expect = 2e-043
Identities = 78/104 (75%), Positives = 83/104 (79%)
Frame = +3 / -3
Query: 1305 SGDCCSMFTSTLQRSASRPTSSEVNPISRQVSRTIFS*STVALVVISPNTITMLVLVQVS 1484
SG CCSM T TLQ SASRPTSSE P+ V RTIFS*ST A VVISP TITMLV VQVS
Sbjct: 423 SGLCCSMLTRTLQLSASRPTSSETKPMFLHVLRTIFS*STFAFVVISPKTITMLVFVQVS 244
Query: 1485 QATLLSGSCARHASSTASDTWSHSLSGCPSFTDSEVKRKLSAAI 1616
ATLLSGSC+R AS TAS+ WS SLSGCPSFTDS+V RK+S AI
Sbjct: 243 HATLLSGSCSRQASRTASEIWSQSLSGCPSFTDSDVYRKVSVAI 112
>gb|AW698879.1|AW698879 r109 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 205
Score = 111 bits (237), Expect = 3e-025
Identities = 49/66 (74%), Positives = 52/66 (78%)
Frame = -2 / +3
Query: 862 TGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYVARQAAKSIVASGLARRCLVQVSYA 683
TGRKIIIDTY GKDPTKVDRSGAY+ RQAAKSIVA+GLARRC+VQVSYA
Sbjct: 3 TGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYA 182
Query: 682 IGVPEP 665
IGVP P
Sbjct: 183 IGVPXP 200
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 10,121,513
Number of Sequences: 7669
Number of extensions: 129844
Number of successful extensions: 1672
Number of sequences better than 1.0e-020: 7
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 473788640
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)