TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2405087.2.1
(737 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF322116.2|AF322116 Medicago sativa sucrose-phosphate sy... 150 3e-075
gb|AY468362.2| Medicago sativa sucrose-phosphate synthase g... 111 3e-069
>gb|AF322116.2|AF322116 Medicago sativa sucrose-phosphate synthase mRNA, complete cds
Length = 3579
Score = 150 bits (323), Expect(3) = 3e-075
Identities = 63/113 (55%), Positives = 79/113 (69%)
Frame = +2 / +2
Query: 398 NNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQIIFEDPEHSSAYC 577
N+ F D SHIEYRWGGEGLRK LV+WA S ++KG + QI+ + S+ YC
Sbjct: 2702 NSEDRLFVGDLYFHSHIEYRWGGEGLRKTLVRWAASTTDKKGESNEQIVSPVEQLSTDYC 2881
Query: 578 LAFRVVNPNHLPPLKELRKLMRIQSLRCNALYNHSATRLSVVPIHASRSQALR 736
AF+V P PPLKELRKLMRIQ+LRC+ +Y + TRL+V+P+ ASRSQALR
Sbjct: 2882 YAFKVRKPGMAPPLKELRKLMRIQALRCHPIYCQNGTRLNVIPVLASRSQALR 3040
Score = 111 bits (237), Expect(3) = 3e-075
Identities = 45/87 (51%), Positives = 60/87 (68%)
Frame = +2 / +2
Query: 131 PISGRKNIIVISVDSVNKEGLVRIIRNAIEVIHKENMSGSTGFVLSTSLTISEIHSLLLS 310
P+ R + VI+VD GL+ +I+ E +E GS GF+LSTS+TISEI S L+S
Sbjct: 2450 PLRSRNRLFVIAVDCDTTSGLLEMIKVIFEAAGEERADGSVGFILSTSMTISEIQSFLIS 2629
Query: 311 GGMLPTDFDAFICNSGSNIYYPSHSRE 391
GG+ P DFDA+ICNSGS++YYPS + E
Sbjct: 2630 GGLSPNDFDAYICNSGSDLYYPSLNSE 2710
Score = 102 bits (218), Expect(3) = 3e-049
Identities = 52/107 (48%), Positives = 62/107 (57%)
Frame = -3 / -1
Query: 735 LRA*ERDAWIGTTDNLVALWLYNALQRRDWILINFLNSFKGGR*LGLTTLNARQYAEECS 556
LRA ERDA G T +LV Y QRR WILIN +SF+GG G TL A Q + E
Sbjct: 3039 LRAWERDARTGITFSLVPF*QYIGWQRRAWILINLRSSFRGGAIPGFRTLKA*Q*SVESC 2860
Query: 555 GSSKIICLSVLPFLSTTEVAHFTRYFLRPSPPQRYSICDW*F*SNAN 415
+ IC + PFLS E A T+ FL PSPPQRYS+ +W + S N
Sbjct: 2859 STGLTICSLLSPFLSVVEAAQRTKVFLNPSPPQRYSM*EWKYKSPTN 2719
Score = 81.3 bits (171), Expect(3) = 3e-049
Identities = 41/79 (51%), Positives = 51/79 (64%)
Frame = -3 / -1
Query: 378 EG**MLLPLLQMKASKSVGSMPPDSSSECISDIVKEVDSTKPVEPDMFSLCMTSIAFLII 199
EG** LPLLQ+ ASKS+G PP+ ++CISDIV EVDS P +P S S LII
Sbjct: 2697 EG**RSLPLLQI*ASKSLGLKPPEIRNDCISDIVIEVDSMNPTDPSALSSPAASNITLII 2518
Query: 198 LTRPSLLTESTEITMIFFL 142
RP ++++ST IT FL
Sbjct: 2517 SRRPEVVSQSTAITNSRFL 2461
Score = 61.6 bits (128), Expect(3) = 3e-075
Identities = 23/32 (71%), Positives = 24/32 (75%)
Frame = +2 / +2
Query: 2 RENGLTNIHQFSWPEHCKNYLSRILTLGPRSP 97
R NGL NIH FSWPEHCK YLS+I T PR P
Sbjct: 2063 RLNGLKNIHLFSWPEHCKTYLSKIATCKPRHP 2158
Score = 52.4 bits (108), Expect(3) = 3e-049
Identities = 23/32 (71%), Positives = 27/32 (84%)
Frame = -3 / -1
Query: 96 GDLGPRVSILDR*FLQCSGHENW*IFVSPFSL 1
G LG +V+ILDR* LQCSGHEN *+F +PFSL
Sbjct: 2157 GCLGLQVAILDR*VLQCSGHENK*MFFNPFSL 2062
>gb|AY468362.2| Medicago sativa sucrose-phosphate synthase gene, promoter region and
complete cds
Length = 8933
Score = 111 bits (237), Expect(4) = 3e-069
Identities = 45/87 (51%), Positives = 60/87 (68%)
Frame = +2 / +3
Query: 131 PISGRKNIIVISVDSVNKEGLVRIIRNAIEVIHKENMSGSTGFVLSTSLTISEIHSLLLS 310
P+ R + VI+VD GL+ +I+ E +E GS GF+LSTS+TISEI S L+S
Sbjct: 7413 PLRSRNRLFVIAVDCDTTSGLLEMIKVIFEAAGEERADGSVGFILSTSMTISEIQSFLIS 7592
Query: 311 GGMLPTDFDAFICNSGSNIYYPSHSRE 391
GG+ P DFDA+ICNSGS++YYPS + E
Sbjct: 7593 GGLSPNDFDAYICNSGSDLYYPSLNSE 7673
Score = 81.3 bits (171), Expect(4) = 3e-069
Identities = 33/68 (48%), Positives = 42/68 (61%)
Frame = +2 / +3
Query: 398 NNSKITFALDQNHQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQIIFEDPEHSSAYC 577
N+ F D SHIEYRWGGEGLRK LV+WA S ++KG + QI+ + S+ YC
Sbjct: 7665 NSEDRLFVGDLYFHSHIEYRWGGEGLRKTLVRWAASTTDKKGESNEQIVSPVEQLSTDYC 7844
Query: 578 LAFRVVNP 601
AF+V P
Sbjct: 7845 YAFKVRKP 7868
Score = 81.3 bits (171), Expect(4) = 4e-044
Identities = 41/79 (51%), Positives = 51/79 (64%)
Frame = -3 / -2
Query: 378 EG**MLLPLLQMKASKSVGSMPPDSSSECISDIVKEVDSTKPVEPDMFSLCMTSIAFLII 199
EG** LPLLQ+ ASKS+G PP+ ++CISDIV EVDS P +P S S LII
Sbjct: 7660 EG**RSLPLLQI*ASKSLGLKPPEIRNDCISDIVIEVDSMNPTDPSALSSPAASNITLII 7481
Query: 198 LTRPSLLTESTEITMIFFL 142
RP ++++ST IT FL
Sbjct: 7480 SRRPEVVSQSTAITNSRFL 7424
Score = 73.9 bits (155), Expect(4) = 3e-069
Identities = 30/42 (71%), Positives = 37/42 (88%)
Frame = +2 / +1
Query: 611 PPLKELRKLMRIQSLRCNALYNHSATRLSVVPIHASRSQALR 736
PPLKELRKLMRIQ+LRC+ +Y + TRL+V+P+ ASRSQALR
Sbjct: 8026 PPLKELRKLMRIQALRCHPIYCQNGTRLNVIPVLASRSQALR 8151
Score = 61.6 bits (128), Expect(4) = 3e-069
Identities = 23/32 (71%), Positives = 24/32 (75%)
Frame = +2 / +3
Query: 2 RENGLTNIHQFSWPEHCKNYLSRILTLGPRSP 97
R NGL NIH FSWPEHCK YLS+I T PR P
Sbjct: 7026 RLNGLKNIHLFSWPEHCKTYLSKIATCKPRHP 7121
Score = 60.2 bits (125), Expect(4) = 4e-044
Identities = 28/62 (45%), Positives = 35/62 (56%)
Frame = -3 / -2
Query: 600 GLTTLNARQYAEECSGSSKIICLSVLPFLSTTEVAHFTRYFLRPSPPQRYSICDW*F*SN 421
G TL A Q + E + IC + PFLS E A T+ FL PSPPQRYS+ +W + S
Sbjct: 7867 GFRTLKA*Q*SVESCSTGLTICSLLSPFLSVVEAAQRTKVFLNPSPPQRYSM*EWKYKSP 7688
Query: 420 AN 415
N
Sbjct: 7687 TN 7682
Score = 52.4 bits (108), Expect(4) = 4e-044
Identities = 23/32 (71%), Positives = 27/32 (84%)
Frame = -3 / -2
Query: 96 GDLGPRVSILDR*FLQCSGHENW*IFVSPFSL 1
G LG +V+ILDR* LQCSGHEN *+F +PFSL
Sbjct: 7120 GCLGLQVAILDR*VLQCSGHENK*MFFNPFSL 7025
Score = 49.2 bits (101), Expect(4) = 4e-044
Identities = 24/42 (57%), Positives = 27/42 (64%)
Frame = -3 / -1
Query: 735 LRA*ERDAWIGTTDNLVALWLYNALQRRDWILINFLNSFKGG 610
LRA ERDA G T +LV Y QRR WILIN +SF+GG
Sbjct: 8150 LRAWERDARTGITFSLVPF*QYIGWQRRAWILINLRSSFRGG 8025
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,688,796
Number of Sequences: 7669
Number of extensions: 94742
Number of successful extensions: 963
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 188073410
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)