TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2404800.2.1
(1228 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF000976.1|MSAF000976 Medicago sativa 7-O-methyltransfer... 196 4e-066
gb|U97125.1|MSU97125 Medicago sativa isoflavone-O-methytran... 195 3e-065
emb|AX050436.1| Sequence 1 from Patent WO0071736 195 3e-065
gb|AF000975.1|MSAF000975 Medicago sativa 7-O-methyltransfer... 195 3e-065
gb|AF023481.1|AF023481 Medicago sativa o-methytransferase (... 104 6e-056
gb|M63853.1|ALFSALMCAT Medicago sativa S-adenosyl-L-methion... 114 2e-030
emb|AX259373.1| Sequence 3 from Patent WO0173090 114 2e-030
gb|L10211.1|ALFIOM Medicago sativa isoliquiritigenin 2'-O-m... 93 2e-028
gb|CO512262.1|CO512262 s13dSG03H0600048_114038 Glandular tr... 95 1e-021
>gb|AF000976.1|MSAF000976 Medicago sativa 7-O-methyltransferase (7-IOMT(9)) mRNA, complete cds
Length = 1227
Score = 196 bits (422), Expect(3) = 4e-066
Identities = 78/186 (41%), Positives = 116/186 (62%)
Frame = -1 / +3
Query: 733 VCDSSFVMDIVVKECGDVFRGLSSLVDVXXXXXXXXXAISTAFPHVQCSVLDLPHVAANA 554
+ S ++++ +++C VF GL S+VDV I FP ++C V D P V N
Sbjct: 525 MASDSKLINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENL 704
Query: 553 PANTSVKYIAGDMFESIPPADAVFLKWVLHDWGDADCIKILKNCKKAIPSRDAGGKVIIV 374
+ ++ Y+ GDMF SIP ADAV LK++LH+W D DC++ILK CK+A+ + GKV I+
Sbjct: 705 SGSNNLTYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTII 884
Query: 373 DMVVGGQSSNIKHKETQVLFDLFIMTINGAERDEHEWKKIISEAGFSDYKIIPVLGVRSI 194
DMV+ + + + ++L D+ + +NG ER+E EWKK+ EAGF YKI P+ G S+
Sbjct: 885 DMVINEKKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSL 1064
Query: 193 IEVYP* 176
IE+YP*
Sbjct: 1065 IEIYP* 1082
Score = 59.7 bits (124), Expect(3) = 4e-066
Identities = 31/72 (43%), Positives = 42/72 (58%)
Frame = -1 / +3
Query: 1222 LLDAQLELWHTSFAFIKSMALKSAVDLGIADAIHSHGGNATLPQIVSRAALHPSKIPCLR 1043
+ AQ L+ +AFI SM+LK AV + I + IH+HG +L +VS + SKI +R
Sbjct: 60 IFKAQALLYKHIYAFIDSMSLKWAVGMNIPNIIHNHGKPISLSNLVSILQVPSSKIGNVR 239
Query: 1042 RLMRVLTAAGIF 1007
RLMR L G F
Sbjct: 240 RLMRYLAHNGFF 275
Score = 36.3 bits (73), Expect(3) = 4e-066
Identities = 15/38 (39%), Positives = 18/38 (47%)
Frame = -1 / +3
Query: 826 DPTLFEMTHGLTAWDLNDHKPAFGELFNQGMVCDSSFV 713
D TLF +T G WD D P + FN M DS +
Sbjct: 435 DLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSKLI 548
>gb|U97125.1|MSU97125 Medicago sativa isoflavone-O-methytransferase mRNA, complete cds
Length = 1231
Score = 195 bits (421), Expect(3) = 3e-065
Identities = 78/186 (41%), Positives = 116/186 (62%)
Frame = -1 / +1
Query: 733 VCDSSFVMDIVVKECGDVFRGLSSLVDVXXXXXXXXXAISTAFPHVQCSVLDLPHVAANA 554
+ S ++++ +++C VF GL S+VDV I FP ++C V D P V N
Sbjct: 526 MASDSKLINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENL 705
Query: 553 PANTSVKYIAGDMFESIPPADAVFLKWVLHDWGDADCIKILKNCKKAIPSRDAGGKVIIV 374
+ ++ Y+ GDMF SIP ADAV LK++LH+W D DC++ILK CK+A+ + GKV I+
Sbjct: 706 SGSNNLTYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTII 885
Query: 373 DMVVGGQSSNIKHKETQVLFDLFIMTINGAERDEHEWKKIISEAGFSDYKIIPVLGVRSI 194
DMV+ + + + ++L D+ + +NG ER+E EWKK+ EAGF YKI P+ G S+
Sbjct: 886 DMVIDKKKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSL 1065
Query: 193 IEVYP* 176
IE+YP*
Sbjct: 1066 IEIYP* 1083
Score = 57.4 bits (119), Expect(3) = 3e-065
Identities = 30/72 (41%), Positives = 42/72 (58%)
Frame = -1 / +1
Query: 1222 LLDAQLELWHTSFAFIKSMALKSAVDLGIADAIHSHGGNATLPQIVSRAALHPSKIPCLR 1043
+ AQ L+ +AFI SM+LK AV++ I + I +HG +L +VS + SKI +R
Sbjct: 61 IFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVSILQVPSSKIGNVR 240
Query: 1042 RLMRVLTAAGIF 1007
RLMR L G F
Sbjct: 241 RLMRYLAHNGFF 276
Score = 36.3 bits (73), Expect(3) = 3e-065
Identities = 15/38 (39%), Positives = 18/38 (47%)
Frame = -1 / +1
Query: 826 DPTLFEMTHGLTAWDLNDHKPAFGELFNQGMVCDSSFV 713
D TLF +T G WD D P + FN M DS +
Sbjct: 436 DLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSKLI 549
>emb|AX050436.1| Sequence 1 from Patent WO0071736
Length = 1231
Score = 195 bits (421), Expect(3) = 3e-065
Identities = 78/186 (41%), Positives = 116/186 (62%)
Frame = -1 / +1
Query: 733 VCDSSFVMDIVVKECGDVFRGLSSLVDVXXXXXXXXXAISTAFPHVQCSVLDLPHVAANA 554
+ S ++++ +++C VF GL S+VDV I FP ++C V D P V N
Sbjct: 526 MASDSKLINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENL 705
Query: 553 PANTSVKYIAGDMFESIPPADAVFLKWVLHDWGDADCIKILKNCKKAIPSRDAGGKVIIV 374
+ ++ Y+ GDMF SIP ADAV LK++LH+W D DC++ILK CK+A+ + GKV I+
Sbjct: 706 SGSNNLTYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTII 885
Query: 373 DMVVGGQSSNIKHKETQVLFDLFIMTINGAERDEHEWKKIISEAGFSDYKIIPVLGVRSI 194
DMV+ + + + ++L D+ + +NG ER+E EWKK+ EAGF YKI P+ G S+
Sbjct: 886 DMVIDKKKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSL 1065
Query: 193 IEVYP* 176
IE+YP*
Sbjct: 1066 IEIYP* 1083
Score = 57.4 bits (119), Expect(3) = 3e-065
Identities = 30/72 (41%), Positives = 42/72 (58%)
Frame = -1 / +1
Query: 1222 LLDAQLELWHTSFAFIKSMALKSAVDLGIADAIHSHGGNATLPQIVSRAALHPSKIPCLR 1043
+ AQ L+ +AFI SM+LK AV++ I + I +HG +L +VS + SKI +R
Sbjct: 61 IFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVSILQVPSSKIGNVR 240
Query: 1042 RLMRVLTAAGIF 1007
RLMR L G F
Sbjct: 241 RLMRYLAHNGFF 276
Score = 36.3 bits (73), Expect(3) = 3e-065
Identities = 15/38 (39%), Positives = 18/38 (47%)
Frame = -1 / +1
Query: 826 DPTLFEMTHGLTAWDLNDHKPAFGELFNQGMVCDSSFV 713
D TLF +T G WD D P + FN M DS +
Sbjct: 436 DLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSKLI 549
>gb|AF000975.1|MSAF000975 Medicago sativa 7-O-methyltransferase (7-IOMT(6)) mRNA, complete cds
Length = 1284
Score = 195 bits (421), Expect(3) = 3e-065
Identities = 78/186 (41%), Positives = 116/186 (62%)
Frame = -1 / +3
Query: 733 VCDSSFVMDIVVKECGDVFRGLSSLVDVXXXXXXXXXAISTAFPHVQCSVLDLPHVAANA 554
+ S ++++ +++C VF GL S+VDV I FP ++C V D P V N
Sbjct: 531 MASDSKLINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENL 710
Query: 553 PANTSVKYIAGDMFESIPPADAVFLKWVLHDWGDADCIKILKNCKKAIPSRDAGGKVIIV 374
+ ++ Y+ GDMF SIP ADAV LK++LH+W D DC++ILK CK+A+ + GKV I+
Sbjct: 711 SGSNNLTYVGGDMFTSIPNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGKRGKVTII 890
Query: 373 DMVVGGQSSNIKHKETQVLFDLFIMTINGAERDEHEWKKIISEAGFSDYKIIPVLGVRSI 194
DMV+ + + + ++L D+ + +NG ER+E EWKK+ EAGF YKI P+ G S+
Sbjct: 891 DMVIDEKKDENQVTQIKLLMDVNMACLNGKERNEEEWKKLFIEAGFQHYKISPLTGFLSL 1070
Query: 193 IEVYP* 176
IE+YP*
Sbjct: 1071 IEIYP* 1088
Score = 57.4 bits (119), Expect(3) = 3e-065
Identities = 30/72 (41%), Positives = 42/72 (58%)
Frame = -1 / +3
Query: 1222 LLDAQLELWHTSFAFIKSMALKSAVDLGIADAIHSHGGNATLPQIVSRAALHPSKIPCLR 1043
+ AQ L+ +AFI SM+LK AV++ I + I +HG +L +VS + SKI +R
Sbjct: 66 IFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVSILQVPSSKIGNVR 245
Query: 1042 RLMRVLTAAGIF 1007
RLMR L G F
Sbjct: 246 RLMRYLAHNGFF 281
Score = 36.3 bits (73), Expect(3) = 3e-065
Identities = 15/38 (39%), Positives = 18/38 (47%)
Frame = -1 / +3
Query: 826 DPTLFEMTHGLTAWDLNDHKPAFGELFNQGMVCDSSFV 713
D TLF +T G WD D P + FN M DS +
Sbjct: 441 DLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSKLI 554
>gb|AF023481.1|AF023481 Medicago sativa o-methytransferase (iomt2003) mRNA, complete cds
Length = 1194
Score = 104 bits (221), Expect(4) = 6e-056
Identities = 41/91 (45%), Positives = 61/91 (67%)
Frame = -1 / +1
Query: 448 DCIKILKNCKKAIPSRDAGGKVIIVDMVVGGQSSNIKHKETQVLFDLFIMTINGAERDEH 269
DC++ILK CK+A+ + GKV I+DMV+ + + + ++L D+ + +NG ER+E
Sbjct: 787 DCLRILKKCKEAVTNDGKRGKVTIIDMVIDEKKDENQVTQIKLLMDVNMACLNGKERNEE 966
Query: 268 EWKKIISEAGFSDYKIIPVLGVRSIIEVYP* 176
EWKK+ EAGF YKI P+ G S+IE+YP*
Sbjct: 967 EWKKLFIEAGFQHYKISPLTGFLSLIEIYP* 1059
Score = 79.9 bits (168), Expect(4) = 6e-056
Identities = 33/86 (38%), Positives = 48/86 (55%)
Frame = -1 / +1
Query: 733 VCDSSFVMDIVVKECGDVFRGLSSLVDVXXXXXXXXXAISTAFPHVQCSVLDLPHVAANA 554
+ S ++++ +++C VF GL S+VDV I FP ++C V D P V N
Sbjct: 529 MASDSKLINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENL 708
Query: 553 PANTSVKYIAGDMFESIPPADAVFLK 476
+ ++ Y+ GDMF SIP ADAV LK
Sbjct: 709 SGSNNLTYVGGDMFTSIPNADAVLLK 786
Score = 57.4 bits (119), Expect(4) = 6e-056
Identities = 30/72 (41%), Positives = 42/72 (58%)
Frame = -1 / +1
Query: 1222 LLDAQLELWHTSFAFIKSMALKSAVDLGIADAIHSHGGNATLPQIVSRAALHPSKIPCLR 1043
+ AQ L+ +AFI SM+LK AV++ I + I +HG +L +VS + SKI +R
Sbjct: 64 IFKAQALLYKHIYAFIDSMSLKWAVEMNIPNIIQNHGKPISLSNLVSILQVPSSKIGNVR 243
Query: 1042 RLMRVLTAAGIF 1007
RLMR L G F
Sbjct: 244 RLMRYLAHNGFF 279
Score = 36.3 bits (73), Expect(4) = 6e-056
Identities = 15/38 (39%), Positives = 18/38 (47%)
Frame = -1 / +1
Query: 826 DPTLFEMTHGLTAWDLNDHKPAFGELFNQGMVCDSSFV 713
D TLF +T G WD D P + FN M DS +
Sbjct: 439 DLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSKLI 552
>gb|M63853.1|ALFSALMCAT Medicago sativa S-adenosyl-L-methionine: caffeic acid
3-0-methyltransferase mRNA, complete cds
Length = 1317
Score = 114 bits (244), Expect(2) = 2e-030
Identities = 44/91 (48%), Positives = 58/91 (63%)
Frame = -1 / +1
Query: 679 FRGLSSLVDVXXXXXXXXXAISTAFPHVQCSVLDLPHVAANAPANTSVKYIAGDMFESIP 500
F GL SLVDV I + +P ++ DLPHV +AP+ V+++ GDMF SIP
Sbjct: 622 FEGLKSLVDVGGGTGAVINTIVSKYPTIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSIP 801
Query: 499 PADAVFLKWVLHDWGDADCIKILKNCKKAIP 407
ADAVF+KW+ HDW D C+K LKNC +A+P
Sbjct: 802 KADAVFMKWICHDWSDEHCLKFLKNCYEALP 894
Score = 33.1 bits (66), Expect(2) = 2e-030
Identities = 12/40 (30%), Positives = 24/40 (60%)
Frame = -1 / +1
Query: 814 FEMTHGLTAWDLNDHKPAFGELFNQGMVCDSSFVMDIVVK 695
F +G+TA++ + P F ++FN+GM S+ M +++
Sbjct: 490 FNKAYGMTAFEYHGTDPRFNKVFNKGMSDHSTITMKKILE 609
>emb|AX259373.1| Sequence 3 from Patent WO0173090
Length = 1098
Score = 114 bits (244), Expect(2) = 2e-030
Identities = 44/91 (48%), Positives = 58/91 (63%)
Frame = -1 / +1
Query: 679 FRGLSSLVDVXXXXXXXXXAISTAFPHVQCSVLDLPHVAANAPANTSVKYIAGDMFESIP 500
F GL SLVDV I + +P ++ DLPHV +AP+ V+++ GDMF SIP
Sbjct: 592 FEGLKSLVDVGGGTGAVINTIVSKYPTIKGINFDLPHVIEDAPSYPGVEHVGGDMFVSIP 771
Query: 499 PADAVFLKWVLHDWGDADCIKILKNCKKAIP 407
ADAVF+KW+ HDW D C+K LKNC +A+P
Sbjct: 772 KADAVFMKWICHDWSDEHCLKFLKNCYEALP 864
Score = 33.1 bits (66), Expect(2) = 2e-030
Identities = 12/40 (30%), Positives = 24/40 (60%)
Frame = -1 / +1
Query: 814 FEMTHGLTAWDLNDHKPAFGELFNQGMVCDSSFVMDIVVK 695
F +G+TA++ + P F ++FN+GM S+ M +++
Sbjct: 460 FNKAYGMTAFEYHGTDPRFNKVFNKGMSDHSTITMKKILE 579
>gb|L10211.1|ALFIOM Medicago sativa isoliquiritigenin 2'-O-methyltransferase mRNA,
complete cds
Length = 1300
Score = 93.2 bits (197), Expect(3) = 2e-028
Identities = 37/90 (41%), Positives = 53/90 (58%)
Frame = -1 / +3
Query: 679 FRGLSSLVDVXXXXXXXXXAISTAFPHVQCSVLDLPHVAANAPANTSVKYIAGDMFESIP 500
F G+S+LVDV I + +P ++ DLP V NAP + ++++ GDMF S+P
Sbjct: 648 FEGISTLVDVGGGSGRNLELIISKYPLIKGINFDLPQVIENAPPLSGIEHVGGDMFASVP 827
Query: 499 PADAVFLKWVLHDWGDADCIKILKNCKKAI 410
DA+ LK V H+W D CI+ L NC KA+
Sbjct: 828 QGDAMILKAVCHNWSDEKCIEFLSNCHKAL 917
Score = 38.6 bits (78), Expect(3) = 2e-028
Identities = 16/44 (36%), Positives = 27/44 (61%)
Frame = -1 / +3
Query: 1048 LRRLMRVLTAAGIFSAAHRSPDDGGGELVYGLTPASQLLVGGSS 917
L R++R+L + + ++ R+ +DGG E VYGL+ + LV S
Sbjct: 282 LDRMLRLLASYSVLTSTTRTIEDGGAERVYGLSMVGKYLVPDES 413
Score = 27.2 bits (53), Expect(3) = 2e-028
Identities = 10/32 (31%), Positives = 17/32 (53%)
Frame = -1 / +3
Query: 826 DPTLFEMTHGLTAWDLNDHKPAFGELFNQGMV 731
D LF+ HG+T ++ ++FN+ MV
Sbjct: 504 DIDLFKNVHGVTKYEFMGKDKKMNQIFNKSMV 599
>gb|CO512262.1|CO512262 s13dSG03H0600048_114038 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 588
Score = 95.0 bits (201), Expect(2) = 1e-021
Identities = 37/86 (43%), Positives = 51/86 (59%)
Frame = -1 / +1
Query: 679 FRGLSSLVDVXXXXXXXXXAISTAFPHVQCSVLDLPHVAANAPANTSVKYIAGDMFESIP 500
F + LVDV I++ +P++Q DLP V +AP V ++ GDMFE IP
Sbjct: 328 FDHIKKLVDVGGGNGINLNLITSKYPYIQGVNFDLPQVIEHAPTYPGVTHVGGDMFEGIP 507
Query: 499 PADAVFLKWVLHDWGDADCIKILKNC 422
DA+FLK +LH+W D C+K+LKNC
Sbjct: 508 KGDAIFLKTILHNWDDEHCLKLLKNC 585
Score = 23.1 bits (44), Expect(2) = 1e-021
Identities = 8/27 (29%), Positives = 13/27 (48%)
Frame = -1 / +1
Query: 814 FEMTHGLTAWDLNDHKPAFGELFNQGM 734
F HG+ ++ P F ++FN M
Sbjct: 196 FNRLHGMHMFEYCRANPKFNDMFNTAM 276
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,167,073
Number of Sequences: 7669
Number of extensions: 112692
Number of successful extensions: 946
Number of sequences better than 1.0e-020: 9
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 335362365
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)