TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2276392.2.1
(644 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514508.1|CO514508 s13dSG43E0900069_327676 Glandular tr... 208 7e-055
gb|AW698456.1|AW698456 g409 glandular-haired subtracted cDN... 133 2e-032
>gb|CO514508.1|CO514508 s13dSG43E0900069_327676 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 532
Score = 208 bits (448), Expect = 7e-055
Identities = 87/96 (90%), Positives = 90/96 (93%)
Frame = +2 / +2
Query: 59 KMVQRLTYRKRHSYATKSNQTRVVKTPGGKLVYQYTKKRASGPKCPVTGKKIQGIPHLRP 238
KMVQRLTYR+RHSYATKSNQ RVVKTPGGKLVYQ TKKRASGPKCPVTGK+IQGIPHLRP
Sbjct: 65 KMVQRLTYRRRHSYATKSNQHRVVKTPGGKLVYQTTKKRASGPKCPVTGKRIQGIPHLRP 244
Query: 239 AEYKRSRLARNQRTVNRPYGGVLSGTAVRERIIRAF 346
EYKRSRL RN+RTVNR YGGVLSG AVRERIIRAF
Sbjct: 245 TEYKRSRLPRNRRTVNRAYGGVLSGGAVRERIIRAF 352
Score = 184 bits (397), Expect = 7e-048
Identities = 88/131 (67%), Positives = 92/131 (70%)
Frame = -3 / -2
Query: 453 RMDSNIVAYIV*LLEALSLVFWIFNTFFTIFCSSTKKARMILSLTAVPDRTPPYGRFTVL 274
R+ S+ V L S F I TFFTIFCSSTKKA MILS TA PD+TPP RFTVL
Sbjct: 459 RLLSHFVVQNYALEATFSFAF*ILRTFFTIFCSSTKKALMILSRTAPPDKTPP*ARFTVL 280
Query: 273 WFLANLDLLYSAGLRCGIPWIFFPVTGHFGPLARFLVYWYTSFPPGVLTTRVWFDLVA*L 94
FL NLDLLYS GL+CGIP I PVTGH GPLA FLV TS PPGVLTT W DLVA*L
Sbjct: 279 RFLGNLDLLYSVGLKCGIP*ILLPVTGHLGPLALFLVV**TSLPPGVLTTLCWLDLVA*L 100
Query: 93 CRFR*VSRCTI 61
R R*V RCTI
Sbjct: 99 *RRR*VRRCTI 67
Score = 116 bits (249), Expect = 2e-027
Identities = 53/97 (54%), Positives = 66/97 (68%)
Frame = -2 / -1
Query: 352 NQKGADDPLPNCSTRQNTAIWTIHSPLVPCQPGPLVLSRSQVWNSLDLLPGDWALRSARA 173
NQ+ +DP PN ++RQN+AI T+HSP V QP LVL R QVWNSL+ L + ALRSA +
Sbjct: 358 NQESPNDPFPNSTSRQNSAISTVHSPTVSRQP*SLVLCRPQVWNSLNSLASNRALRSASS 179
Query: 172 LLGVLVHKFPTRCLDDAGLVRFGGVAVPLPVGEPLHH 62
LL L++K TR D+ LV FG VAV +GE LHH
Sbjct: 178 LLSSLINKLTTRGFDNPVLVGFGCVAVTTAIGETLHH 68
Score = 109 bits (233), Expect = 3e-025
Identities = 48/116 (41%), Positives = 71/116 (61%)
Frame = +1 / +1
Query: 61 DGAAAHLPEAAQLRHQIEPDPRRQDTWWETCVPVHQEARERTEVPSHREEDPRNSTPETC 240
+GA +HL ++QLR+QI+P Q+ WW C+ + E RT VP + +E+ RNST E
Sbjct: 67 NGATSHLSPSSQLRNQIQPTQGCQNPWW*ACLSDY*EES*RT*VPCYWQENSRNSTLEAY 246
Query: 241 *VQEVQVGKEPKDCESSIWRCSVWYCS*GEDHPRLFG*GTEDSEEGVEDPKDQG*G 408
VQE++V +P DCE +WR VW C G+DH G* +D E+G ++ + +G G
Sbjct: 247 RVQEIKVA*KP*DCEPCLWRSFVWRCCSGKDH*GFLG*RAKDCEKGPQNSEGKGKG 414
>gb|AW698456.1|AW698456 g409 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 220
Score = 133 bits (286), Expect = 2e-032
Identities = 55/62 (88%), Positives = 57/62 (91%)
Frame = +2 / -1
Query: 59 KMVQRLTYRKRHSYATKSNQTRVVKTPGGKLVYQYTKKRASGPKCPVTGKKIQGIPHLRP 238
KMVQRLTYR+R YATKSNQ RVVKTPGGKLVYQ TKKRASGPKCPVTGK+IQGIPHLRP
Sbjct: 187 KMVQRLTYRRRLRYATKSNQHRVVKTPGGKLVYQTTKKRASGPKCPVTGKRIQGIPHLRP 8
Query: 239 AE 244
E
Sbjct: 7 TE 2
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,480,944
Number of Sequences: 7669
Number of extensions: 71221
Number of successful extensions: 1043
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 159352840
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)