TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 2192909.2.3
         (674 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|M63853.1|ALFSALMCAT  Medicago sativa S-adenosyl-L-methion...   174   9e-045
emb|AX259373.1|  Sequence 3 from Patent WO0173090                 174   9e-045
gb|AJ888485.1|AJ888485  AJ888485 Medicago sativa subsp. x va...   169   4e-043

>gb|M63853.1|ALFSALMCAT Medicago sativa S-adenosyl-L-methionine: caffeic acid
            3-0-methyltransferase mRNA, complete cds
          Length = 1317

 Score =  174 bits (375), Expect = 9e-045
 Identities = 65/107 (60%), Positives = 81/107 (75%)
 Frame = -1 / +1

                                                                        
Query: 674  PAGDAILMKWILHDWSDAHCATLLKNCYDALPENGKVIVVECVLPVNTEATPKAQGVFHV 495
            P  DA+ MKWI HDWSD HC   LKNCY+ALP+NGKVIV EC+LPV  +++   +GV H+
Sbjct: 799  PKADAVFMKWICHDWSDEHCLKFLKNCYEALPDNGKVIVAECILPVAPDSSLATKGVVHI 978

                                                           
Query: 494  DMIMLAHNPGGKERYEREFRELAKGAGFSGFKATYIYANAWAIEFIK 354
            D+IMLAHNPGGKER ++EF +LAKGAGF GFK      N + +EF+K
Sbjct: 979  DVIMLAHNPGGKERTQKEFEDLAKGAGFQGFKVHCNAFNTYIMEFLK 1119




 Score =  100 bits (214), Expect = 1e-022
 Identities = 51/104 (49%), Positives = 59/104 (56%)
 Frame = +1 / -2

                                                                        
Query: 355  LMNSXXXXXXXXXXXXNPEKPAPLASSRNSRSYRSLPPGLCASMIMSTWNTPCALGVASV 534
            L NS             P  PAPLA S NS    S PPGL A+MI S   TP    + S 
Sbjct: 1118 LRNSMMYVLKALQWTLKPWNPAPLARSSNSFCVLSFPPGL*ANMITSM*TTPFVARLESG 939

                                                        
Query: 535  LTGSTHSTTMTLPFSGSAS*QFLSSVAQCASLQSWRIHFMRMAS 666
             TGS HS T+T P SGSAS*QF  +  QC+SLQS +IHF++ AS
Sbjct: 938  ATGSMHSATITFPLSGSAS*QFFKNFKQCSSLQS*QIHFIKTAS 807



>emb|AX259373.1| Sequence 3 from Patent WO0173090
          Length = 1098

 Score =  174 bits (375), Expect = 9e-045
 Identities = 65/107 (60%), Positives = 81/107 (75%)
 Frame = -1 / +1

                                                                        
Query: 674  PAGDAILMKWILHDWSDAHCATLLKNCYDALPENGKVIVVECVLPVNTEATPKAQGVFHV 495
            P  DA+ MKWI HDWSD HC   LKNCY+ALP+NGKVIV EC+LPV  +++   +GV H+
Sbjct: 769  PKADAVFMKWICHDWSDEHCLKFLKNCYEALPDNGKVIVAECILPVAPDSSLATKGVVHI 948

                                                           
Query: 494  DMIMLAHNPGGKERYEREFRELAKGAGFSGFKATYIYANAWAIEFIK 354
            D+IMLAHNPGGKER ++EF +LAKGAGF GFK      N + +EF+K
Sbjct: 949  DVIMLAHNPGGKERTQKEFEDLAKGAGFQGFKVHCNAFNTYIMEFLK 1089




 Score =  100 bits (214), Expect = 1e-022
 Identities = 51/104 (49%), Positives = 59/104 (56%)
 Frame = +1 / -2

                                                                        
Query: 355  LMNSXXXXXXXXXXXXNPEKPAPLASSRNSRSYRSLPPGLCASMIMSTWNTPCALGVASV 534
            L NS             P  PAPLA S NS    S PPGL A+MI S   TP    + S 
Sbjct: 1088 LRNSMMYVLKALQWTLKPWNPAPLARSSNSFCVLSFPPGL*ANMITSM*TTPFVARLESG 909

                                                        
Query: 535  LTGSTHSTTMTLPFSGSAS*QFLSSVAQCASLQSWRIHFMRMAS 666
             TGS HS T+T P SGSAS*QF  +  QC+SLQS +IHF++ AS
Sbjct: 908  ATGSMHSATITFPLSGSAS*QFFKNFKQCSSLQS*QIHFIKTAS 777



>gb|AJ888485.1|AJ888485 AJ888485 Medicago sativa subsp. x varia 4-day-old Medicago sativa
           subsp. x varia cDNA, mRNA sequence
          Length = 546

 Score =  169 bits (363), Expect = 4e-043
 Identities = 66/109 (60%), Positives = 80/109 (73%)
 Frame = -1 / +2

                                                                       
Query: 674 PAGDAILMKWILHDWSDAHCATLLKNCYDALPENGKVIVVECVLPVNTEATPKAQGVFHV 495
           PAGDAI MKWILHDWSD HC  LLKNCY A+PE+GKVIVV+ +LPV  E T  A+     
Sbjct: 65  PAGDAIFMKWILHDWSDEHCLKLLKNCYKAIPEDGKVIVVDTILPVMPETTANAKSACMS 244

                                                            
Query: 494 DMIMLAHNPGGKERYEREFRELAKGAGFSGFKATYIYANAWAIEFIK*I 348
           D++M+  NPGGKER E+EF+ELAKG+GFSG K     +  W +EF K*+
Sbjct: 245 DVLMMTQNPGGKERTEQEFKELAKGSGFSGIKPICCVSGLWVMEFFK*L 391




 Score = 95.0 bits (201), Expect = 9e-021
 Identities = 50/107 (46%), Positives = 59/107 (55%)
 Frame = +1 / -1

                                                                       
Query: 352 HLMNSXXXXXXXXXXXXNPEKPAPLASSRNSRSYRSLPPGLCASMIMSTWNTPCALGVAS 531
           +L NS             P  P PLA+S NS S RS PPG    +  S      AL V S
Sbjct: 387 YLKNSITHNPDTQHIGLMPLNPDPLANSLNSCSVRSFPPGF*VIIKTSDMQADFALAVVS 208

                                                          
Query: 532 VLTGSTHSTTMTLPFSGSAS*QFLSSVAQCASLQSWRIHFMRMASPA 672
            +TG   STT+T P SG A *QF +S  QC+SLQS  IHF++MASPA
Sbjct: 207 GMTGRIVSTTITFPSSGIAL*QFFNSFKQCSSLQS*SIHFIKMASPA 67



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 4,504,376
Number of Sequences: 7669
Number of extensions: 56727
Number of successful extensions: 900
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 168617540
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)