TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 2169937.2.1
         (891 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AY804253.1|  Medicago sativa acidic glucanase (Glu) mRNA,...   117   2e-036
gb|U27179.1|MSU27179  Medicago sativa acidic glucanase mRNA,...    59   3e-031

>gb|AY804253.1| Medicago sativa acidic glucanase (Glu) mRNA, complete cds
          Length = 1113

 Score =  117 bits (251), Expect(3) = 2e-036
 Identities = 50/81 (61%), Positives = 58/81 (71%)
 Frame = +2 / +1

                                                                       
Query: 383 YNNLFDAMVDAVVAALEKAGAPNVRVVVSEXGWPSAGGFGASVDNARKYNQGLIDHVGRG 562
           Y NLFDAM+D+V AAL+  G   V VVVSE GWPS GG   S DNAR Y   LI HVG+G
Sbjct: 730 YQNLFDAMLDSVHAALDNTGIGWVNVVVSESGWPSDGGAATSYDNARIYLDNLIRHVGKG 909

                                
Query: 563 TPKRTGPLETFVFAMFNENQK 625
           TP+R    ET++FAMF+ENQK
Sbjct: 910 TPRRPWATETYIFAMFDENQK 972




 Score = 34.1 bits (68), Expect(3) = 2e-036
 Identities = 12/16 (75%), Positives = 14/16 (87%)
 Frame = +1 / +1

                           
Query: 277 NVYPYFAYRDNPRDIS 324
           NVYPYF++  NPRDIS
Sbjct: 625 NVYPYFSHVGNPRDIS 672




 Score = 33.1 bits (66), Expect(3) = 2e-036
 Identities = 12/19 (63%), Positives = 14/19 (73%)
 Frame = +2 / +1

                               
Query: 641  EKNFGLFYGNKQPVYPIRF 697
            EK+FG+FY NKQ  YP  F
Sbjct: 985  EKHFGVFYPNKQKKYPFGF 1041



>gb|U27179.1|MSU27179 Medicago sativa acidic glucanase mRNA, complete cds
          Length = 1374

 Score = 59.3 bits (123), Expect(4) = 3e-031
 Identities = 25/38 (65%), Positives = 28/38 (73%)
 Frame = +2 / +1

                                                 
Query: 383 YNNLFDAMVDAVVAALEKAGAPNVRVVVSEXGWPSAGG 496
           Y NLFDAM+D+V AAL+  G   V VVVSE GWPS GG
Sbjct: 781 YQNLFDAMLDSVHAALDNTGIGWVNVVVSESGWPSDGG 894




 Score = 58.3 bits (121), Expect(4) = 3e-031
 Identities = 24/44 (54%), Positives = 30/44 (68%)
 Frame = +2 / +1

                                                        
Query: 494  GFGASVDNARKYNQGLIDHVGRGTPKRTGPLETFVFAMFNENQK 625
            G   S DNAR Y   LI + G+GTP+R    ET++FAMF+ENQK
Sbjct: 889  GGATSYDNARIYLDNLIRYEGKGTPRRPWATETYIFAMFDENQK 1020




 Score = 34.1 bits (68), Expect(4) = 3e-031
 Identities = 12/16 (75%), Positives = 14/16 (87%)
 Frame = +1 / +1

                           
Query: 277 NVYPYFAYRDNPRDIS 324
           NVYPYF++  NPRDIS
Sbjct: 676 NVYPYFSHVGNPRDIS 723




 Score = 33.1 bits (66), Expect(4) = 3e-031
 Identities = 12/19 (63%), Positives = 14/19 (73%)
 Frame = +2 / +1

                               
Query: 641  EKNFGLFYGNKQPVYPIRF 697
            EK+FG+FY NKQ  YP  F
Sbjct: 1033 EKHFGVFYPNKQKKYPFGF 1089



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 5,157,635
Number of Sequences: 7669
Number of extensions: 53315
Number of successful extensions: 596
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 232456653
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)